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Protein Page:
ATR (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ATR a ser/thr kinase of the PIKK family most closely related to ATM. Activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]Q. Phosphorylates BRCA1, Chk1, MCM2, RAD17, RPA2, SMC1 and p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Kinase, lipid; Protein kinase, atypical; Membrane protein, integral; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; ATYPICAL group; PIKK family; ATR subfamily
Cellular Component: nucleoplasm; XY body; PML body; chromosome
Molecular Function: protein serine/threonine kinase activity; protein binding; DNA binding; MutLalpha complex binding; MutSalpha complex binding; ATP binding; protein kinase activity
Biological Process: response to drug; peptidyl-serine phosphorylation; negative regulation of DNA replication; regulation of protein binding; protein amino acid autophosphorylation; multicellular organismal development; DNA damage checkpoint; positive regulation of DNA damage response, signal transduction by p53 class mediator; cell cycle; DNA repair; DNA replication; response to DNA damage stimulus
Reference #:  Q13535 (UniProtKB)
Alt. Names/Synonyms: ataxia telangiectasia and Rad3 related; Ataxia telangiectasia and Rad3-related protein; ATR; FRAP-related protein 1; FRAP-related protein-1; FRP1; MEC1; MEC1, mitosis entry checkpoint 1, homolog; protein kinase ATR; Rad3 related protein; SCKL; SCKL1; Serine/threonine-protein kinase ATR
Gene Symbols: ATR
Molecular weight: 301,367 Da
Basal Isoelectric point: 7.17  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  G1/S Checkpoint  |  G2/M DNA Damage Checkpoint  |  Mitochondrial Control of Apoptosis
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ATR

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cell growth, altered: S428‑p
enzymatic activity, induced: S428‑p, T1989‑p
molecular association, regulation: T1989‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 Y26-p GSATPEEyNTVVQkP
0 1 K32-ub EyNTVVQkPRQILCQ
0 1 S61-p ELVKKTDsQPTSVML
1 0 Y291-p DTDQLKLyEEPLSKL
1 0 Y310-p FPFEAEAyRNIEPVy
0 1 Y317-p yRNIEPVyLNMLLEK
9 6 S428-p SSNSDGIsPKRRRLs
1 82 S435-p sPKRRRLsssLNPsk
0 47 S436-p PKRRRLsssLNPskR
0 5 S437-p KRRRLsssLNPskRA
0 2 S441-p LsssLNPskRAPKQT
0 1 K442-ub sssLNPskRAPKQTE
0 1 T533-p KKPSVVItWMSLDFY
0 1 S580 LIYMQVNSSFEDHIL
0 1 S581 IYMQVNSSFEDHILE
0 1 K866-ub ISRNNELkDTLILTT
0 2 K1005-ub LLPDLAAkAsPAASA
0 1 S1007-p PDLAAkAsPAASALI
0 1 N1024 LGKQLNVNRREILIN
0 1 Y1122-p SPELMADyLQPKLLG
0 1 S1140-p FFNMQLLsSSVGIED
0 1 K1173-ac HVSSVRVkMMTTLRT
0 1 T1275-p LQEYRKEtSESTDLQ
0 1 Y1312 TSLKETLYKNQEKLI
1 0 S1333-p ETVEPIIsQLVTVLL
1 0 T1566-p DDQHTINtQDIASDL
1 0 T1578-p SDLCQLStQTVFSML
1 0 T1589-p FSMLDHLtQWARHKF
0 1 K1613-ub HSKSNRNkVDSMVST
0 4 S1645-p QDTLAVAsFRSKAYT
0 1 K1703-ub RKAEPSLkEQILEHE
0 2 K1824-ub TAFYDSLkLVRAEQI
0 1 S1871-p IKPLFQHsPGDSsQE
0 2 S1876-p QHsPGDSsQEDSLNW
6 29 T1989-p LCFPENEtPPEGKNM
0 1 S2186-p MTAVSKSsyPMRVNR
0 1 Y2187-p TAVSKSsyPMRVNRC
0 1 T2219 VGDATRLTDKLLELC
0 1 S2313-p LQKPKKIsLKGSDGK
0 1 K2587-ub TGEVVNEkAKTHVLD
0 1 K2604-ub QRLQGVIkTRNRVTG
2853 : Phospho-ATR (Ser428) Antibody
  mouse

 
Y26 GSATPEEYNTVVQKP
K32 EYNTVVQKPRQILCQ
A61 ELVKKTDAQPTSVML
Y294 DADQLKLYEEPLSKL
Y313 FPFEAEAYRNIEPVY
Y320 YRNIEPVYLNVLLEK
S431-p SGNNDEVsPKRRKLs
S438-p sPKRRKLsSSLSSYK
S439 PKRRKLsSSLSSYKK
S440 KRRKLsSSLSSYKKP
Y444 LsSSLSSYKKPSRQP
K445 sSSLSSYKKPSRQPE
T536 KKPPVVVTWMSLDFY
S583-p LMYMQVKssFKDHVL
S584-p MYMQVKssFKDHVLE
K866 IARNNELKDTLILTT
K1005 LLPDLAAKASPAASA
S1007 PDLAAKASPAASALI
S1024-p LGKQLNVsRREILIN
Y1122 SPELMADYLQPKLLG
S1140 FFNMQLLSSSVGIED
K1173 HVSSVRVKMMTTLRT
T1275 LQEYRKETSETTDLQ
Y1312-p TSLKETLyKNQEKLI
S1333 ETVEPVISQLVTVIL
T1566 DEQHAISTQDSASDL
T1578 SDLCQLSTQTVFSVL
T1589 FSVLDHLTQWARHKF
- gap
S1639 QDTLAVASFRSKAYT
K1697 RKAEPSLKEQILEHE
K1818 TTFYDTLKLVRAEQI
S1865 LKPLFRKSPGDSCNE
C1870 RKSPGDSCNEDSLNW
S1983 LCFPENKSPSESKHM
S2180 MTAVSKSSYPMRVNR
Y2181 TAVSKSSYPMRVNRC
T2213-p VGDATRLtDKLLELC
S2304 LQKPKKISLKGSDGK
K2578 TGEVVNEKAKTHVLD
K2595 QRLQGVIKTRNRVTG
2853 : Phospho-ATR (Ser428) Antibody
  rat

 
Y26 GSATPEEYNTVVQKP
K32 EYNTVVQKPRQILCQ
T61 ELVKKTDTQPTSVML
Y294 DADQLKLYEEPLSKL
Y313 FPFEAEAYRNIEPVY
Y320 YRNIEPVYLNVLLEK
S431 RGSNDGVSPKRRRLS
S438 SPKRRRLSSSLSSYK
S439 PKRRRLSSSLSSYKR
S440 KRRRLSSSLSSYKRP
Y444 LSSSLSSYKRPSRQS
K445 SSSLSSYKRPSRQSE
T536 KKPPVVITWMSLDFY
S583 LMYIQVKSSFQDHVL
S584 MYIQVKSSFQDHVLE
K867 ISRSNELKDTLILTT
K1006 LLPDLAAKASPAASA
S1008 PDLAAKASPAASALI
N1025 LGKQLNVNRREILIN
Y1123 SPELMADYLQPKLLG
S1141 FFNMQLLSSSVGIED
K1174 HVSSVRVKMMTTLRT
T1276 LQEYRKETSESTDLQ
Y1313 TSLKETLYKNQEKLI
S1334 ETVEPVISQLVTVIL
T1567 DDQHAISTQDSASDL
T1579 SDLCQLSTQTVFSML
T1590 FSMLDHLTQWARHKF
K1614 QNKPKGGKVNYPVSS
S1646 QDTLAVASFRSKAYT
K1704 RKAEPSLKEQILEHE
K1825 TAFYDTLKLVRAEQI
F1872 LKPLFHKFPGDSCNE
C1877 HKFPGDSCNEDSLNW
S1990 LCFPENKSPTESKHM
S2187 MTAVSKSSYPMRVNR
Y2188 TAVSKSSYPMRVNRC
T2220 VGDATRLTDKLLELC
S2311 LQKPKKISLKGSDGK
K2585 TGEVVNEKAKTHVLD
K2602 QRLQGVIKTRNRVTG
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