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Protein Page:
BUB3 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
BUB3 Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Belongs to the WD repeat BUB3 family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Cell cycle regulation
Cellular Component: kinetochore; nucleolus; nucleus; cytosol
Molecular Function: protein binding
Biological Process: meiosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic sister chromatid segregation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; regulation of chromosome segregation; mitotic cell cycle; attachment of spindle microtubules to kinetochore
Reference #:  O43684 (UniProtKB)
Alt. Names/Synonyms: BUB3; BUB3 budding uninhibited by benzimidazoles 3 homolog; BUB3L; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog (yeast); hBUB3; mitotic checkpoint component; Mitotic checkpoint protein BUB3
Gene Symbols: BUB3
Molecular weight: 37,155 Da
Basal Isoelectric point: 6.36  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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BUB3

Protein Structure Not Found.


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Sites Implicated In
apoptosis, induced: S19‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
1 0 S19-p PPEDGISsVkFSPNT
0 1 K21-ub EDGISsVkFSPNTSQ
0 1 S33-p TSQFLLVsSWDTSVR
0 6 T127-p VKLWDPRtPCNAGTF
0 7 K139-ub GTFSQPEkVyTLsVS
1 4 Y141-p FSQPEkVyTLsVSGD
0 1 S144-p PEkVyTLsVSGDRLI
0 1 T154-p GDRLIVGtAGRRVLV
0 4 K179-ac QRRESSLkYQTRCIR
0 7 K191-ub CIRAFPNkQGyVLSS
1 1 Y194-p AFPNkQGyVLSSIEG
1 3 Y207-p EGRVAVEyLDPsPEV
0 7 S211-p AVEyLDPsPEVQkkK
0 1 K216-ac DPsPEVQkkKYAFKC
0 32 K216-ub DPsPEVQkkKYAFKC
0 13 K217-ub PsPEVQkkKYAFKCH
0 9 K322-ub QVTDAETkPksPCt_
0 2 K324-ub TDAETkPksPCt___
0 2 S325-p DAETkPksPCt____
0 1 T328-p TkPksPCt_______
  BUB3 iso2  
S19 PPEDGISSVKFSPNT
K21 EDGISSVKFSPNTSQ
S33 TSQFLLVSSWDTSVR
T127 VKLWDPRTPCNAGTF
K139 GTFSQPEKVYTLSVS
Y141 FSQPEKVYTLSVSGD
S144 PEKVYTLSVSGDRLI
T154 GDRLIVGTAGRRVLV
K179 QRRESSLKYQTRCIR
K191 CIRAFPNKQGYVLSS
Y194 AFPNKQGYVLSSIEG
Y207 EGRVAVEYLDPSPEV
S211 AVEYLDPSPEVQKKK
K216 DPSPEVQKKKYAFKC
K216 DPSPEVQKKKYAFKC
K217 PSPEVQKKKYAFKCH
K322 QVTDAETKPKST___
K324 TDAETKPKST_____
S325 DAETKPKST______
T326 AETKPKST_______
  mouse

 
S19 PPEDGISSVKFSPNT
K21 EDGISSVKFSPNTSQ
S33 TSQFLLVSSWDTSVR
T127 VKLWDPRTPCNAGTF
K139 GTFSQPEKVYTLSVS
Y141 FSQPEKVYTLSVSGD
S144 PEKVYTLSVSGDRLI
T154 GDRLIVGTAGRRVLV
K179-ac QRRESSLkYQTRCIR
K191 CIRAFPNKQGYVLSS
Y194 AFPNKQGYVLSSIEG
Y207 EGRVAVEYLDPsPEV
S211-p AVEYLDPsPEVQkkK
K216 DPsPEVQKkKYAFKC
K216-ub DPsPEVQkkKYAFKC
K217-ub PsPEVQkkKYAFKCH
K322 QVTDAETKPKST___
K324 TDAETKPKST_____
S325 DAETKPKST______
T326 AETKPKST_______
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