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Bub3 (human)

Overview Bub3 Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Belongs to the WD repeat BUB3 family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB. Protein type: Cell cycle regulation Cellular Component: kinetochore; nucleolus; nucleus; cytosol Molecular Function: protein binding Biological Process: meiosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic sister chromatid segregation; regulation of chromosome segregation; attachment of spindle microtubules to kinetochore; M phase of mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; cell cycle checkpoint; mitotic prometaphase Reference #:  O43684 (UniProtKB) Alt. Names/Synonyms: BUB3; BUB3 budding uninhibited by benzimidazoles 3 homolog; BUB3L; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog (yeast); hBUB3; mitotic checkpoint component; Mitotic checkpoint protein BUB3 Gene Symbols: BUB3 Molecular weight: 37,155 Da Basal Isoelectric point: 6.36  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below Bub3

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	Sites Implicated In
apoptosis, induced: S19‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
1	0		S19-p	PPEDGISsVkFSPNT
0	1		K21-u	EDGISsVkFSPNTSQ
0	1		S33-p	TSQFLLVsSWDTSVR
0	5		T127-p	VKLWDPRtPCNAGTF
0	7		K139-u	GTFSQPEkVyTLSVS
0	4		Y141-p	FSQPEkVyTLSVSGD
0	1		T154-p	GDRLIVGtAGRRVLV
0	3		K179-a	QRRESSLkYQTRCIR
0	7		K191-u	CIRAFPNkQGyVLSS
0	1		Y194-p	AFPNkQGyVLSSIEG
0	2		Y207-p	EGRVAVEyLDPsPEV
0	5		S211-p	AVEyLDPsPEVQkkK
0	32		K216-u	DPsPEVQkkKYAFKC
0	13		K217-u	PsPEVQkkKYAFKCH
0	9		K322-u	QVTDAETkPksPCt_
0	2		K324-u	TDAETkPksPCt___
0	1		S325-p	DAETkPksPCt____
0	1		T328-p	TkPksPCt_______

	mouse
S19	PPEDGISSVKFSPNT
K21	EDGISSVKFSPNTSQ
S33	TSQFLLVSSWDTSVR
T127	VKLWDPRTPCNAGTF
K139	GTFSQPEKVYTLSVS
Y141	FSQPEKVYTLSVSGD
T154	GDRLIVGTAGRRVLV
K179	QRRESSLKYQTRCIR
K191	CIRAFPNKQGYVLSS
Y194	AFPNKQGYVLSSIEG
Y207	EGRVAVEYLDPsPEV
S211-p	AVEYLDPsPEVQkkK
K216-u	DPsPEVQkkKYAFKC
K217-u	PsPEVQkkKYAFKCH
K322	QVTDAETKPKST___
K324	TDAETKPKST_____
S325	DAETKPKST______
T326	AETKPKST_______

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