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Protein Page:
ASK1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ASK1 a protein kinase of the STE11 family. Phosphorylates and activates two different subgroups of MAP kinase kinases, MKK4/SEK1 and MKK3/MKK6, which in turn activate stress-activated protein kinase (JNK) and p38 subgroups of MAP kinases, respectively. Overexpression induces apoptotic cell death. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE11 family
Cellular Component: endoplasmic reticulum; cytosol
Molecular Function: protein binding; protein homodimerization activity; MAP kinase kinase kinase activity; magnesium ion binding; caspase activator activity; protein kinase binding; protein phosphatase binding; ATP binding; protein kinase activity
Biological Process: caspase activation; viral reproduction; activation of MAPKK activity; positive regulation of apoptosis; MAPKKK cascade; positive regulation of caspase activity; innate immune response; JNK cascade; induction of apoptosis by oxidative stress; protein amino acid phosphorylation; activation of JNK activity
Reference #:  Q99683 (UniProtKB)
Alt. Names/Synonyms: apoptosis signal regulating kinase 1; apoptosis signal-regulating kinase 1; ASK-1; ASK1; MAP/ERK kinase kinase 5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; MEKK5; mitogen-activated protein kinase kinase kinase 5; Mitogen-activated protein kinase kinase kinase 5 variant
Gene Symbols: MAP3K5
Molecular weight: 154,537 Da
Basal Isoelectric point: 5.52  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  Apoptosis Regulation  |  B Cell Receptor Signaling  |  Death Receptor Signaling  |  Regulation of P38 MAPKs  |  SAPK/JNK Signaling Cascades
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ASK1

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
apoptosis, altered: S83‑p, T838‑p, S966‑p, S1033‑p
apoptosis, induced: S966‑p
apoptosis, inhibited: S83‑p, S966‑p
transcription, altered: S83‑p, T813‑p, T838‑p, T842‑p, S966‑p
enzymatic activity, induced: T838‑p
enzymatic activity, inhibited: S83‑p, S966‑p, S1033‑p
molecular association, regulation: Y718‑p, T838‑p, S966‑p
phosphorylation: S83‑p
protein degradation: Y718‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 2 - gap
0 2 - gap
14 0 S83-p ATRGRGSsVGGGSRR
0 1 S425 WFKKAFESEPTLQSG
0 1 S431 ESEPTLQSGINYAVL
0 1 S461-p KVGVKLSsLLGKKGN
0 1 T528-p YKHFVKLtTEQPVAk
1 0 K535-sm tTEQPVAkQELVDFW
0 1 Y570-p ILEPTKIyQPSyLSI
0 1 Y574-p TKIyQPSyLSINNEV
0 2 Y691 VVLGKGTYGIVYAGR
1 0 Y718-p IPERDSRySQPLHEE
1 0 T813-p GDNVLINtySGVLKI
0 2 Y814-p DNVLINtySGVLKIs
0 1 S821-p ySGVLKIsDFGtSKR
0 1 T825-p LKIsDFGtSKRLAGI
0 1 T836 LAGINPCTEtFTGtL
13 2 T838-p GINPCTEtFTGtLQY
2 0 T842-p CTEtFTGtLQYMAPE
0 1 K946-ac KVSSKKKkTQPKLSA
0 1 A956 PKLSALSAGsNEYLR
0 8 S958-p LSALSAGsNEYLRsI
0 1 S964-p GsNEYLRsIsLPVPV
12 2 S966-p NEYLRsIsLPVPVLV
0 1 Y982-p DTSSSSEyGSVsPDT
0 2 S986-p SSEyGSVsPDTELKV
0 10 S1029-p DENFEDHsAPPsPEE
1 14 S1033-p EDHsAPPsPEEKDSG
0 2 T1059 ATLHRILTEDQDKIV
0 1 S1072 IVRNLMESLAQGAEE
1 0 K1083-sm GAEEPKLkWEHITTL
1 0 T1109-p DRKIIATtLSKLkLE
1 0 K1114-sm ATtLSKLkLELDFDS
0 1 S1240-p DSQSAHRsLNVQLGR
0 2 S1303 LPVFHLNSSGTNTED
1 0 T1326-p VNGADEDtISRFLAE
3761 : Phospho-ASK1 (Ser83) Antibody
3765 : Phospho-ASK1 (Thr845) Antibody
3764 : Phospho-ASK1 (Ser967) Antibody
  ASK1 iso5  
S55-p CLARRARssPELWLA
S56-p LARRARssPELWLAR
S163 ATRGRGSSVGGGSRR
S505 WFKKAFESEPTLQSG
S511 ESEPTLQSGINYAVL
S541 KVGVKLSSLLGKKGN
T608 YKHFVKLTTEQPVAK
K615 TTEQPVAKQELVDFW
Y650 ILEPTKIYQPSYLSI
Y654 TKIYQPSYLSINNEV
Y771 VVLGKGTYGIVYAGR
Y798 IPERDSRYSQPLHEE
T893 GDNVLINTYSGVLKI
Y894 DNVLINTYSGVLKIS
S901 YSGVLKISDFGTSKR
T905 LKISDFGTSKRLAGI
T916 LAGINPCTETFTGTL
T918 GINPCTETFTGTLQY
T922 CTETFTGTLQYMAPE
K1026 KVSSKKKKTQPKLSA
A1036 PKLSALSAGSNEYLR
S1038 LSALSAGSNEYLRSI
S1044 GSNEYLRSISLPVPV
S1046 NEYLRSISLPVPVLV
Y1062 DTSSSSEYGSVSPDD
S1066 SSEYGSVSPDDTELK
S1110 DENFEDHSAPPSPEE
S1114 EDHSAPPSPEEKDSG
T1140 ATLHRILTEDQDKIV
S1153 IVRNLMESLAQGAEE
K1164 GAEEPKLKWEHITTL
T1190 DRKIIATTLSKLKLE
K1195 ATTLSKLKLELDFDS
S1321 DSQSAHRSLNVQLGR
S1384 LPVFHLNSSGTNTED
T1407 VNGADEDTISRFLAE
  mouse

 
- gap
- gap
S90-p ATRGRGNsGSGGGRR
S432-p WFKKAFEsEPTLQsG
S438-p EsEPTLQsGINYAVL
S468 KVGVKLSSLLGKKGN
T535 YKHFVKLTTEQPSAK
K542 TTEQPSAKQELVDFW
Y577 ILEPTKIYQPSYLSI
Y581 TKIYQPSYLSINNEV
Y698 VVLGKGTYGIVYAGR
Y725 IPERDSRYSQPLHEE
T820 GDNVLINTYSGVLKI
Y821 DNVLINTYSGVLKIS
S828 YSGVLKISDFGTSKR
T832 LKISDFGTSKRLAGI
T843-p LAGINPCtEtFTGtL
T845-p GINPCtEtFTGtLQY
T849-p CtEtFTGtLQYMAPE
K953 KVSSKKKKTQPKLSA
T963-p PKLSALStGsNEYLR
S965-p LSALStGsNEYLRSI
S971 GsNEYLRSIsLPVPV
S973-p NEYLRSIsLPVPVLV
Y989 DTSSSSEYGSVSPDT
S993 SSEYGSVSPDTELKA
S1036-p DENFEDHsAPPsPEE
S1040-p EDHsAPPsPEEKDSG
T1066-p ATLHRILtEDQDKVV
S1079-p VVRNLMEsLAQGAEE
K1090 GAEEPKLKWEHITTL
T1116 DRKIIATTLSKLKLE
K1121 ATTLSKLKLELDFDS
S1247 DSQNAHRSLNVQLGR
S1310-p FPVCHLNsPGTTTED
T1333 ENGADEDTISRFLAE
3765 : Phospho-ASK1 (Thr845) Antibody
3764 : Phospho-ASK1 (Ser967) Antibody
  rat

 
- gap
- gap
S54 ATRGRGNSGSGGGRR
S396 WFKKAFESEPTLQSG
S402 ESEPTLQSGINYAVL
S432 KVGVKLSSLLGKKGN
T499 YKHFVKLTTEQPTAK
K506 TTEQPTAKQELVDFW
Y541 ILEPTKIYQPSYLSI
Y545 TKIYQPSYLSINNEV
Y662-p VVLGKGTyGIVYAGR
Y689 IPERDSRYSQPLHEE
T784 GDNVLINTYSGVLKI
Y785 DNVLINTYSGVLKIS
S792 YSGVLKISDFGTSKR
T796 LKISDFGTSKRLAGI
T807 LAGINPCTETFTGTL
T809 GINPCTETFTGTLQY
T813 CTETFTGTLQYMAPE
K917 KVSSKKKKTQPKLSE
- gap
- gap
S928 KLSEYLRSISLPVPV
S930 SEYLRSISLPVPVLV
Y946 DTSSSSEYGSVsPDT
S950-p SSEYGSVsPDTELKA
S993 DENFEDHSAPPSPEE
S997 EDHSAPPSPEEKDSG
T1023 ATLHRILTEDQDKVV
S1036 VVRNLMESLAQGPEE
K1047 GPEEPKLKWEHITTL
T1073 DRKIIATTLSRLKLE
K1078 ATTLSRLKLELDFDS
S1204 DSQNAHRSLNVQLGR
A1267 FPVCHLNAPGTAIED
T1290 ENGADEDTISRFLAE
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