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mTOR (mouse)

Overview mTOR an atypical kinase belonging to the PIKK family of kinases. Is the catalytic subunit of two protein complexes, mTORC1 and mTORC2. mTORC1 activates S6K and inactivates 4E-BP1, up-regulating protein synthesis. mTORC1 contains Raptor, a positive regulatory subunit and scaffold for recruiting substrates, two negative regulators, PRAS40 and DEPTOR, and mLST8; it is a target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. mTORC2, a downstream effector of PI3K, is insensitive to rapamycin and activates Akt by phosphorylating a key activation site. mTORC2 contains regulatory subunits Rictor and mSIN1, PROTOR, mLST8, and the negative regulator DEPTOR. mTORC1 suppresses PI3K activity via a strong negative feedback loop that involves S6K1. Inhibiting mTORC1 ablates this negative feedback loop and potentiates PI3K signaling. Known inhibitors of mTOR include rapamycin, temsirolimus (CCI-779). Note: This description may include information from UniProtKB. Protein type: EC 2.7.11.1; Autophagy; Protein kinase, Ser/Thr (non-receptor); Protein kinase, ATYPICAL; Motility/polarity/chemotaxis; Kinase, protein; ATYPICAL group; PIKK family; FRAP subfamily Cellular Component: Golgi apparatus; PML body; mitochondrion; lysosome; endoplasmic reticulum; dendrite; endomembrane system; cytosol; mitochondrial outer membrane; membrane; cell soma; cytoplasm; TORC2 complex; nucleus Molecular Function: transferase activity; protein domain specific binding; protein serine/threonine kinase activity; protein binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; phosphoprotein binding; ribosome binding; kinase activity; drug binding; phosphotransferase activity, alcohol group as acceptor; ATP binding Biological Process: negative regulation of autophagy; TOR signaling pathway; positive regulation of translation; protein amino acid autophosphorylation; regulation of glycogen biosynthetic process; negative regulation of cell size; protein amino acid phosphorylation; germ cell development; response to insulin stimulus; negative regulation of macroautophagy; cellular response to nutrient levels; cell projection organization and biogenesis; positive regulation of stress fiber formation; regulation of carbohydrate utilization; cell growth; regulation of Rac GTPase activity; regulation of response to food; peptidyl-threonine phosphorylation; regulation of carbohydrate metabolic process; positive regulation of lipid biosynthetic process; response to amino acid stimulus; negative regulation of NFAT protein import into nucleus; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; peptidyl-serine phosphorylation; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; ruffle organization and biogenesis; positive regulation of transcription from RNA polymerase III promoter; regulation of fatty acid beta-oxidation; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; regulation of protein kinase activity; phosphorylation Reference #:  Q9JLN9 (UniProtKB) Alt. Names/Synonyms: 2610315D21Rik; AI327068; angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein (FRAP); FKBP-rapamycin-associated protein FRAP; FKBP12-rapamycin complex-associated protein; flat; Frap; Frap1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); MGC118056; mTOR; OTTMUSP00000011776; RAFT1; Rapamycin target protein 1; RAPT1; Serine/threonine-protein kinase mTOR Gene Symbols: Mtor Molecular weight: 288,789 Da Basal Isoelectric point: 6.73  Predict pI for various phosphorylation states CST Pathways:  AMPK Signaling  |  Angiogenesis  |  Autophagy Signaling  |  B Cell Receptor Signaling  |  ErbB/HER Signaling  |  Insulin Receptor Signaling  |  Jak/Stat/IL-6 Signaling  |  mTOR Signaling  |  PI3K/Akt Signaling  |  T Cell Receptor Signaling  |  TGF-ß Signaling  |  Translation: eIF4E and p70S6K  |  Warburg Effect Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below mTOR 1AUE - A/B=2015-2114 (human) 1FAP - B=2018-2112 (human) 1NSG - B=2019-2112 (human) 2FAP - B=2019-2112 (human) 2GAQ - A=2015-2114 (human) 2NPU - A=2015-2114 (human) 2RSE - B=2019-2112 (human) 3FAP - B=2019-2112 (human) 4DRH - A/D=1-140 (human) 4DRI - A=1-140 (human) 4DRJ - A=1-140 (human) 4FAP - B=2019-2112 (human) 4JSN - A/B=1376-2549 (human) 4JSP - A/B=1376-2549 (human) 4JSV - A/B=1376-2549 (human) 4JSX - A/B=1376-2549 (human) 4JT5 - A/B=1376-2549 (human) 4JT6 - A/B=1376-2549 (human) Substrate Sequence Logo

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	Sites Implicated In
enzymatic activity, induced: S2481‑p intracellular localization: S2448‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			mouse
0	1		S92	GGILAIASLIGVEGG
0	1		T102	GVEGGNSTRIGRFAN
0	2		Y110	RIGRFANYLRNLLPS
0	1		K230-u	REPKEMQkPQWYRHT
0	2		K243-u	HTFEEAEkGFDETLA
0	1		K251	GFDETLAKEKGMNRD
0	1		K298-u	QQQLVHDkYCKDLMG
0	1		T308	KDLMGFGTKPRHITP
0	1		T314	GTKPRHITPFTSFQA
0	3		S567	GLAHQLASPGLTTLP
0	1		S719	ICTVGRLSSMNPAFV
0	2		K898-u	LGALDPYkHkVNIGM
0	2		K900-u	ALDPYkHkVNIGMID
0	1		S1131	APEVPLPSRKAALET
0	4		T1162-p	IIHPIVRtLDQSPEL
0	2		S1166	IVRtLDQSPELRSTA
0	2		S1171	DQSPELRSTAMDTLS
0	2		T1172	QSPELRSTAMDTLSS
0	3		K1218	VLICRIVKGYTLADE
0	2		K1218-u	VLICRIVkGYTLADE
0	15		Y1232	EEEDPLIYQHRMLRS
0	1		K1256	PVETGPMKkLHVsTI
0	2		K1256-u	PVETGPMkkLHVsTI
0	1		K1257-u	VETGPMkkLHVsTIN
1	11		S1261-p	PMkkLHVsTINLQKA
0	1		S1288	LEWLRRLSLELLkDS
0	3		K1293-u	RLSLELLkDSSSPSL
0	1		S1297	ELLkDSSSPSLRSCW
0	1		S1299	LkDSSSPSLRSCWAL
0	1		K1400-u	YAKALHYkELEFQkG
0	2		K1406-u	YkELEFQkGPTPAIL
0	1		S1415-p	PTPAILEsLIsINNK
0	2		S1418-p	AILEsLIsINNKLQQ
0	1		S1430-p	LQQPEAAsGVLEYAM
0	1		K1465-u	DALVAYDkKMDTNkE
0	1		K1471-u	DkKMDTNkEDPELML
0	1		T1502	QQCCEKWTLVNDETQ
0	1		K1511-u	VNDETQAkMARMAAA
0	2		K1655-u	EDMRTWLkYASLCGK
0	12		Y1656	DMRTWLkYASLCGKS
0	2		S1821	KKKLRHASGANITNA
0	2		T1870	SPLQKKVTEDLSKTL
0	1		S1874	KKVTEDLSKTLLLYT
0	1		T1876	VTEDLSKTLLLYTVP
0	1		Y1880	LSKTLLLYTVPAVQG
0	1		S1893	QGFFRSISLSRGNNL
0	2		K2066-u	ERGPQTLkETSFNQA
1	0		S2159	RIQSIAPSLQVITSK
1	0		T2164	APSLQVITSKQRPRK
0	1		Y2275	MLRMAPDYDHLTLMQ
0	1		K2370-u	EVAMTREkFPEKIPF
0	3		S2442-p	NTKGNKRsRtRtDsy
0	10		T2444-p	KGNKRsRtRtDsysA
4	13		T2446-p	NKRsRtRtDsysAGQ
91	89		S2448-p	RsRtRtDsysAGQsV
0	38		Y2449-p	sRtRtDsysAGQsVE
0	26		S2450-p	RtRtDsysAGQsVEI
1	36		S2454-p	DsysAGQsVEILDGV
0	1		A2471	GEPAHKKAGTTVPEs
1	3		T2473	PAHKKAGTTVPEsIH
1	2		T2474	AHKKAGTTVPEsIHs
1	10		S2478-p	AGTTVPEsIHsFIGD
35	11		S2481-p	TVPEsIHsFIGDGLV
0	1		K2496-u	KPEALNKkAIQIINR

	human
S92-p	GGILAIAsLIGVEGG
T102-p	GVEGGNAtRIGRFAN
Y110-p	RIGRFANyLRNLLPS
K230	REPKEMQKPQWYRHT
K243-u	HTFEEAEkGFDETLA
K251-u	GFDETLAkEKGMNRD
K298	QQQLVHDKYCKDLMG
T308-p	KDLMGFGtKPRHItP
T314-p	GtKPRHItPFTSFQA
S567-p	GLAHQLAsPGLTTLP
S719-p	ICTVGRLsSMNPAFV
K898-u	LGALDPYkHkVNIGM
K900-u	ALDPYkHkVNIGMID
S1131-p	APEAPLPsRKAALET
T1162-p	IIHPIVRtLDQsPEL
S1166-p	IVRtLDQsPELRstA
S1171-p	DQsPELRstAMDTLS
T1172-p	QsPELRstAMDTLSS
K1218-a	VLICRIVkGYTLADE
K1218-u	VLICRIVkGYTLADE
Y1232-p	EEEDPLIyQHRMLRS
K1256-a	PVETGPMkKLHVsTI
K1256-u	PVETGPMkKLHVsTI
K1257	VETGPMkKLHVsTIN
S1261-p	PMkKLHVsTINLQKA
S1288-p	LEWLRRLsLELLkDS
K1293-u	RLsLELLkDSSsPsL
S1297-p	ELLkDSSsPsLRSCW
S1299-p	LkDSSsPsLRSCWAL
K1400	YAKALHYKELEFQkG
K1406-u	YKELEFQkGPTPAIL
S1415	PTPAILESLISINNK
S1418	AILESLISINNKLQQ
A1430	LQQPEAAAGVLEYAM
K1465	DALVAYDKKMDTNKD
K1471	DKKMDTNKDDPELML
T1502-p	QQCCEKWtLVNDETQ
K1511	VNDETQAKMARMAAA
K1655-u	EDMRTWLkYASLCGK
Y1656	DMRTWLkYASLCGKS
S1821-p	KKKLRHAsGANITNA
T1870-p	SPLQKKVtEDLsKtL
S1874-p	KKVtEDLsKtLLMyT
T1876-p	VtEDLsKtLLMyTVP
Y1880-p	LsKtLLMyTVPAVQG
S1893-p	QGFFRSIsLSRGNNL
K2066-u	ERGPQTLkETSFNQA
S2159-p	RIQSIAPsLQVItSK
T2164-p	APsLQVItSKQRPRK
Y2275	MLRMAPDYDHLTLMQ
K2370	EVAMTREKFPEKIPF
S2442-p	NTKGNKRsRtRtDsy
T2444-p	KGNKRsRtRtDsysA
T2446-p	NKRsRtRtDsysAGQ
S2448-p	RsRtRtDsysAGQsV
Y2449-p	sRtRtDsysAGQsVE
S2450-p	RtRtDsysAGQsVEI
S2454-p	DsysAGQsVEILDGV
T2471-p	GEPAHKKtGttVPEs
T2473-p	PAHKKtGttVPEsIH
T2474-p	AHKKtGttVPEsIHs
S2478-p	tGttVPEsIHsFIGD
S2481-p	tVPEsIHsFIGDGLV
K2496	KPEALNKKAIQIINR

	rat
S92	GGILAIASLIGVEGG
T102	GVEGGNSTRIGRFAN
Y110	RIGRFANYLRNLLPS
K230	REPKEMQKPQWYRHT
K243	HTFEEAEKGFDETLA
K251	GFDETLAKEKGMNRD
K298	QQQLVHDKYCKDLMG
T308	KDLMGFGTKPRHITP
T314	GTKPRHITPFTSFQA
S567	GLAHQLASPGLTTLP
S719	ICTVGRLSSMNPAFV
K898	LGALDPYKHKVNIGM
K900	ALDPYKHKVNIGMID
S1131	APEVPLPSRKAALET
T1162	IIHPIVRTLDQSPEL
S1166	IVRTLDQSPELRSTA
S1171	DQSPELRSTAMDTLS
T1172	QSPELRSTAMDTLSS
K1218	VLICRIVKGYTLADE
K1218	VLICRIVKGYTLADE
Y1232	EEEDPLIYQHRMLRS
K1256	PVETGPMKKLHVsTI
K1256	PVETGPMKKLHVsTI
K1257	VETGPMKKLHVsTIN
S1261-p	PMKKLHVsTINLQKA
S1288	LEWLRRLSLELLKDS
K1293	RLSLELLKDSSSPSL
S1297	ELLKDSSSPSLRSCW
S1299	LKDSSSPSLRSCWAL
K1400	YAKALHYKELEFQKG
K1406	YKELEFQKGPTPAIL
S1415	PTPAILESLISINNK
S1418	AILESLISINNKLQQ
S1430	LQQPEAASGVLEYAM
K1465	DALVAYDKKMDTNKD
K1471	DKKMDTNKDDPELML
T1502	QQCCEKWTLVNDETQ
K1511	VNDETQAKMARMAAA
K1655	EDMRTWLKyASLCGK
Y1656-p	DMRTWLKyASLCGKS
S1821	KKKLRHASGANITNA
T1870	SPLQKKVTEDLSKTL
S1874	KKVTEDLSKTLLLYT
T1876	VTEDLSKTLLLYTVP
Y1880	LSKTLLLYTVPAVQG
S1893	QGFFRSISLSRGNNL
K2066	ERGPQTLKETSFNQA
S2159	RIQSIAPSLQVITSK
T2164	APSLQVITSKQRPRK
Y2275-p	MLRMAPDyDHLTLMQ
K2370	EVAMTREKFPEKIPF
S2442	NAKGNKRSRTRTDsY
T2444	KGNKRSRTRTDsYSA
T2446	NKRSRTRTDsYSAGQ
S2448-p	RSRTRTDsYSAGQSV
Y2449	SRTRTDsYSAGQSVE
S2450	RTRTDsYSAGQSVEI
S2454	DsYSAGQSVEILDGV
T2471	GEPAHKKTGTTVPES
T2473	PAHKKTGTTVPESIH
T2474	AHKKTGTTVPESIHs
S2478	TGTTVPESIHsFIGD
S2481-p	TVPESIHsFIGDGLV
K2496	KPEALNKKAIQIINR

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