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Protein Page:
mTOR (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
mTOR an atypical kinase belonging to the PIKK family of kinases. Is the catalytic subunit of two protein complexes, mTORC1 and mTORC2. mTORC1 activates S6K and inactivates 4E-BP1, up-regulating protein synthesis. mTORC1 contains Raptor, a positive regulatory subunit and scaffold for recruiting substrates, two negative regulators, PRAS40 and DEPTOR, and mLST8; it is a target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. mTORC2, a downstream effector of PI3K, is insensitive to rapamycin and activates Akt by phosphorylating a key activation site. mTORC2 contains regulatory subunits Rictor and mSIN1, PROTOR, mLST8, and the negative regulator DEPTOR. mTORC1 suppresses PI3K activity via a strong negative feedback loop that involves S6K1. Inhibiting mTORC1 ablates this negative feedback loop and potentiates PI3K signaling. Known inhibitors of mTOR include rapamycin, temsirolimus (CCI-779). Note: This description may include information from UniProtKB.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Autophagy; Protein kinase, atypical; EC 2.7.11.1; Motility/polarity/chemotaxis; ATYPICAL group; PIKK family; FRAP subfamily
Cellular Component: Golgi membrane; mitochondrial outer membrane; PML body; endoplasmic reticulum membrane; membrane; lysosome; lysosomal membrane; cytoplasm; TORC2 complex; endomembrane system; cytosol; phosphoinositide 3-kinase complex
Molecular Function: protein domain specific binding; protein serine/threonine kinase activity; protein binding; phosphoprotein binding; ribosome binding; drug binding; kinase activity; ATP binding
Biological Process: negative regulation of autophagy; TOR signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of translation; protein amino acid autophosphorylation; regulation of glycogen biosynthetic process; negative regulation of cell size; signal transduction; protein amino acid phosphorylation; germ cell development; negative regulation of macroautophagy; cellular response to nutrient levels; positive regulation of stress fiber formation; regulation of carbohydrate utilization; response to stress; protein catabolic process; cell growth; regulation of Rac GTPase activity; response to nutrient; regulation of response to food; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; peptidyl-threonine phosphorylation; positive regulation of lipid biosynthetic process; negative regulation of NFAT protein import into nucleus; response to amino acid stimulus; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; peptidyl-serine phosphorylation; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; insulin receptor signaling pathway; ruffle organization and biogenesis; regulation of fatty acid beta-oxidation; positive regulation of transcription from RNA polymerase III promoter; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; regulation of protein kinase activity; phosphorylation; growth
Reference #:  P42345 (UniProtKB)
Alt. Names/Synonyms: FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; FLJ44809; FRAP; FRAP1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1; Serine/threonine-protein kinase mTOR
Gene Symbols: MTOR
Molecular weight: 288,892 Da
Basal Isoelectric point: 6.73  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

mTOR

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
enzymatic activity, induced: S2448‑p, S2481‑p
intracellular localization: S2448‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S92-p GGILAIAsLIGVEGG
0 1 T102-p GVEGGNAtRIGRFAN
0 3 Y110-p RIGRFANyLRNLLPS
0 1 K230 REPKEMQKPQWYRHT
0 2 K243-ub HTFEEAEkGFDETLA
0 1 K251-ub GFDETLAkEKGMNRD
0 1 K298 QQQLVHDKYCKDLMG
0 1 T308-p KDLMGFGtKPRHItP
0 1 T314-p GtKPRHItPFTSFQA
0 5 S567-p GLAHQLAsPGLTTLP
0 1 T665-p KLLVVGItDPDPDIR
0 1 S719-p ICTVGRLsSMNPAFV
0 2 K898-ub LGALDPYkHkVNIGM
0 2 K900-ub ALDPYkHkVNIGMID
0 1 S909-p NIGMIDQsRDASAVS
0 1 S918-p DASAVSLsESKSSQD
0 1 S1131-p APEAPLPsRKAALET
0 5 T1162-p IIHPIVRtLDQsPEL
0 2 S1166-p IVRtLDQsPELRstA
0 2 S1171-p DQsPELRstAMDTLS
0 2 T1172-p QsPELRstAMDTLSS
0 1 Y1188-p VFQLGKKyQIFIPMV
0 4 K1218-ac VLICRIVkGYTLADE
0 2 K1218-ub VLICRIVkGYTLADE
0 16 Y1232-p EEEDPLIyQHRMLRS
0 1 K1256-ac PVETGPMkKLHVstI
0 2 K1256-ub PVETGPMkKLHVstI
0 1 K1257 VETGPMkKLHVstIN
1 17 S1261-p PMkKLHVstINLQKA
0 1 T1262-p MkKLHVstINLQKAW
0 1 S1288-p LEWLRRLsLELLkDS
0 3 K1293-ub RLsLELLkDSSsPsL
0 1 S1297-p ELLkDSSsPsLRSCW
0 1 S1299-p LkDSSsPsLRSCWAL
0 1 K1400 YAKALHYKELEFQkG
0 2 K1406-ub YKELEFQkGPTPAIL
1 1 S1415-p PTPAILEsLIsINNK
1 4 S1418-p AILEsLIsINNKLQQ
0 1 A1430 LQQPEAAAGVLEYAM
0 1 K1465 DALVAYDKKMDTNKD
0 1 K1471 DKKMDTNKDDPELML
0 1 T1502-p QQCCEKWtLVNDETQ
0 1 K1511 VNDETQAKMARMAAA
0 2 K1655-ub EDMRTWLkYASLCGK
0 12 Y1656 DMRTWLkYASLCGKS
0 2 S1821-p KKKLRHAsGANITNA
0 1 S1859-p EAESTENsPTPSPLQ
0 2 T1870-p SPLQKKVtEDLsKtL
0 1 S1874-p KKVtEDLsKtLLMyT
0 1 T1876-p VtEDLsKtLLMyTVP
0 1 Y1880-p LsKtLLMyTVPAVQG
0 1 S1893-p QGFFRSIsLSRGNNL
0 2 K2066-ub ERGPQTLkETSFNQA
0 1 S2155-p QPIIRIQsIAPsLQV
1 1 S2159-p RIQsIAPsLQVItSK
1 0 T2164-p APsLQVItSKQRPRK
0 1 K2370 EVAMTREKFPEKIPF
0 1 T2380-p EKIPFRLtRMLTNAM
0 1 T2434-p LNWRLMDtNtKGNKR
0 1 T2436-p WRLMDtNtKGNKRsR
0 3 S2442-p NtKGNKRsRtRtDsy
0 12 T2444-p KGNKRsRtRtDsysA
4 16 T2446-p NKRsRtRtDsysAGQ
100 107 S2448-p RsRtRtDsysAGQsV
0 43 Y2449-p sRtRtDsysAGQsVE
0 30 S2450-p RtRtDsysAGQsVEI
1 40 S2454-p DsysAGQsVEILDGV
0 1 T2471-p GEPAHKKtGttVPEs
1 3 T2473-p PAHKKtGttVPEsIH
1 3 T2474-p AHKKtGttVPEsIHs
1 14 S2478-p tGttVPEsIHsFIGD
38 15 S2481-p tVPEsIHsFIGDGLV
0 1 K2496 KPEALNKKAIQIINR
2971 : Phospho-mTOR (Ser2448) Antibody
2976 : Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific)
5536 : Phospho-mTOR (Ser2448) (D9C2) XP(R) Rabbit mAb
2974 : Phospho-mTOR (Ser2481) Antibody
  mouse

 
S92 GGILAIASLIGVEGG
T102 GVEGGNSTRIGRFAN
Y110 RIGRFANYLRNLLPS
K230-ub REPKEMQkPQWYRHT
K243-ub HTFEEAEkGFDETLA
K251 GFDETLAKEKGMNRD
K298-ub QQQLVHDkYCKDLMG
T308 KDLMGFGTKPRHITP
T314 GTKPRHITPFTSFQA
S567 GLAHQLASPGLTTLP
T665 KLLVVGITDPDPDIR
S719 ICTVGRLSSMNPAFV
K898-ub LGALDPYkHkVNIGM
K900-ub ALDPYkHkVNIGMID
S909 NIGMIDQSRDASAVS
S918 DASAVSLSESKSSQD
S1131 APEVPLPSRKAALET
T1162-p IIHPIVRtLDQSPEL
S1166 IVRtLDQSPELRSTA
S1171 DQSPELRSTAMDTLS
T1172 QSPELRSTAMDTLSS
Y1188 VFQLGKKYQIFIPMV
K1218 VLICRIVKGYTLADE
K1218-ub VLICRIVkGYTLADE
Y1232 EEEDPLIYQHRMLRS
K1256 PVETGPMKkLHVstI
K1256-ub PVETGPMkkLHVstI
K1257-ub VETGPMkkLHVstIN
S1261-p PMkkLHVstINLQKA
T1262-p MkkLHVstINLQKAW
S1288 LEWLRRLSLELLkDS
K1293-ub RLSLELLkDSSSPSL
S1297 ELLkDSSSPSLRSCW
S1299 LkDSSSPSLRSCWAL
K1400-ub YAKALHYkELEFQkG
K1406-ub YkELEFQkGPTPAIL
S1415-p PTPAILEsLIsINNK
S1418-p AILEsLIsINNKLQQ
S1430-p LQQPEAAsGVLEYAM
K1465-ub DALVAYDkKMDTNkE
K1471-ub DkKMDTNkEDPELML
T1502 QQCCEKWTLVNDETQ
K1511-ub VNDETQAkMARMAAA
K1655-ub EDMRTWLkYASLCGK
Y1656 DMRTWLkYASLCGKS
S1821 KKKLRHASGANITNA
S1859 EAESNENSPTPSPLQ
T1870 SPLQKKVTEDLSKTL
S1874 KKVTEDLSKTLLLYT
T1876 VTEDLSKTLLLYTVP
Y1880 LSKTLLLYTVPAVQG
S1893 QGFFRSISLSRGNNL
K2066-ub ERGPQTLkETSFNQA
S2155 QPIIRIQSIAPSLQV
S2159 RIQSIAPSLQVITSK
T2164 APSLQVITSKQRPRK
K2370-ub EVAMTREkFPEKIPF
T2380 EKIPFRLTRMLTNAM
T2434 LNWRLMDTNTKGNKR
T2436 WRLMDTNTKGNKRsR
S2442-p NTKGNKRsRtRtDsy
T2444-p KGNKRsRtRtDsysA
T2446-p NKRsRtRtDsysAGQ
S2448-p RsRtRtDsysAGQsV
Y2449-p sRtRtDsysAGQsVE
S2450-p RtRtDsysAGQsVEI
S2454-p DsysAGQsVEILDGV
A2471 GEPAHKKAGTTVPEs
T2473 PAHKKAGTTVPEsIH
T2474 AHKKAGTTVPEsIHs
S2478-p AGTTVPEsIHsFIGD
S2481-p TVPEsIHsFIGDGLV
K2496-ub KPEALNKkAIQIINR
2971 : Phospho-mTOR (Ser2448) Antibody
2976 : Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific)
5536 : Phospho-mTOR (Ser2448) (D9C2) XP(R) Rabbit mAb
2974 : Phospho-mTOR (Ser2481) Antibody
  rat

 
S92 GGILAIASLIGVEGG
T102 GVEGGNSTRIGRFAN
Y110 RIGRFANYLRNLLPS
K230 REPKEMQKPQWYRHT
K243 HTFEEAEKGFDETLA
K251 GFDETLAKEKGMNRD
K298 QQQLVHDKYCKDLMG
T308 KDLMGFGTKPRHITP
T314 GTKPRHITPFTSFQA
S567 GLAHQLASPGLTTLP
T665 KLLVVGITDPDPDIR
S719 ICTVGRLSSMNPAFV
K898 LGALDPYKHKVNIGM
K900 ALDPYKHKVNIGMID
S909 NIGMIDQSRDASAVS
S918 DASAVSLSESKSSQD
S1131 APEVPLPSRKAALET
T1162 IIHPIVRTLDQSPEL
S1166 IVRTLDQSPELRSTA
S1171 DQSPELRSTAMDTLS
T1172 QSPELRSTAMDTLSS
Y1188 VFQLGKKYQIFIPMV
K1218-ac VLICRIVkGYTLADE
K1218 VLICRIVKGYTLADE
Y1232 EEEDPLIYQHRMLRS
K1256 PVETGPMKKLHVsTI
K1256 PVETGPMKKLHVsTI
K1257 VETGPMKKLHVsTIN
S1261-p PMKKLHVsTINLQKA
T1262 MKKLHVsTINLQKAW
S1288 LEWLRRLSLELLKDS
K1293 RLSLELLKDSSSPSL
S1297 ELLKDSSSPSLRSCW
S1299 LKDSSSPSLRSCWAL
K1400 YAKALHYKELEFQKG
K1406 YKELEFQKGPTPAIL
S1415 PTPAILESLISINNK
S1418 AILESLISINNKLQQ
S1430 LQQPEAASGVLEYAM
K1465 DALVAYDKKMDTNKD
K1471 DKKMDTNKDDPELML
T1502 QQCCEKWTLVNDETQ
K1511 VNDETQAKMARMAAA
K1655 EDMRTWLKyASLCGK
Y1656-p DMRTWLKyASLCGKS
S1821 KKKLRHASGANITNA
S1859 EAESNESSPTPSPLQ
T1870 SPLQKKVTEDLSKTL
S1874 KKVTEDLSKTLLLYT
T1876 VTEDLSKTLLLYTVP
Y1880 LSKTLLLYTVPAVQG
S1893 QGFFRSISLSRGNNL
K2066 ERGPQTLKETSFNQA
S2155 QPIIRIQSIAPSLQV
S2159 RIQSIAPSLQVITSK
T2164 APSLQVITSKQRPRK
K2370 EVAMTREKFPEKIPF
T2380 EKIPFRLTRMLTNAM
T2434 LNWRLMDTNAKGNKR
A2436 WRLMDTNAKGNKRSR
S2442 NAKGNKRSRTRTDsY
T2444 KGNKRSRTRTDsYSA
T2446 NKRSRTRTDsYSAGQ
S2448-p RSRTRTDsYSAGQSV
Y2449 SRTRTDsYSAGQSVE
S2450 RTRTDsYSAGQSVEI
S2454 DsYSAGQSVEILDGV
T2471 GEPAHKKTGTTVPEs
T2473 PAHKKTGTTVPEsIH
T2474 AHKKTGTTVPEsIHs
S2478-p TGTTVPEsIHsFIGD
S2481-p TVPEsIHsFIGDGLV
K2496 KPEALNKKAIQIINR
2971 : Phospho-mTOR (Ser2448) Antibody
2976 : Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific)
5536 : Phospho-mTOR (Ser2448) (D9C2) XP(R) Rabbit mAb
2974 : Phospho-mTOR (Ser2481) Antibody
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