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Protein Page:
4E-BP1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
4E-BP1 binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway. Note: This description may include information from UniProtKB.
Protein type: Translation initiation; Translation
Cellular Component: protein complex; cytoplasm; nucleolus; nucleus; cytosol
Molecular Function: protein binding; translation repressor activity; eukaryotic initiation factor 4E binding
Biological Process: negative regulation of translational initiation; TOR signaling pathway; response to ethanol; cellular protein metabolic process; translation; insulin receptor signaling pathway; translational initiation; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; lung development; G1/S transition of mitotic cell cycle
Reference #:  Q13541 (UniProtKB)
Alt. Names/Synonyms: 4E-BP1; 4EBP1; BP-1; eIF4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; MGC4316; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1
Gene Symbols: EIF4EBP1
Molecular weight: 12,580 Da
Basal Isoelectric point: 5.32  Predict pI for various phosphorylation states
CST Pathways:  AMPK Signaling  |  Angiogenesis  |  Insulin Receptor Signaling  |  mTOR Signaling  |  PI3K/Akt Signaling  |  Translation: eIF4E and p70S6K
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

4E-BP1

Protein Structure Not Found.


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Sites Implicated In
cell growth, induced: T37‑p, T41‑p, T46‑p, T50‑p
translation, altered: T37‑p, T46‑p, S65‑p, T70‑p
translation, induced: T41‑p, T50‑p
activity, inhibited: T37‑p, T46‑p, S65‑p, T70‑p
molecular association, regulation: T37‑p, T46‑p, S65‑p, T70‑p, S112‑p
phosphorylation: T37‑p, T41‑p, T46‑p, T50‑p, S65‑p, T70‑p, S112‑p
protein conformation: S65‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 S2-p ______MsGGsSCsQ
0 2 S5-p ___MsGGsSCsQtPs
0 1 S8-p MsGGsSCsQtPsRAI
0 2 T10-p GGsSCsQtPsRAIPA
0 1 S12-p sSCsQtPsRAIPATR
0 1 D25-ca TRRVVLGdGVQLPPG
0 46 Y34-p VQLPPGDysttPGGt
0 35 S35-p QLPPGDysttPGGtL
1 31 T36-p LPPGDysttPGGtLF
90 115 T37-p PPGDysttPGGtLFs
2 71 T41-p ysttPGGtLFsttPG
0 56 S44-p tPGGtLFsttPGGtR
2 67 T45-p PGGtLFsttPGGtRI
91 113 T46-p GGtLFsttPGGtRII
2 53 T50-p FsttPGGtRIIyDRK
0 28 Y54-p PGGtRIIyDRKFLME
0 1 K57 tRIIyDRKFLMECRN
64 33 S65-p FLMECRNsPVtktPP
0 4 T68-p ECRNsPVtktPPRDL
0 1 K69-ub CRNsPVtktPPRDLP
38 35 T70-p RNsPVtktPPRDLPt
0 4 T77-p tPPRDLPtIPGVtsP
0 3 T82-p LPtIPGVtsPssDEP
4 7 S83-p PtIPGVtsPssDEPP
0 2 S85-p IPGVtsPssDEPPME
0 3 S86-p PGVtsPssDEPPMEA
1 8 S94-p DEPPMEAsQsHLRNs
0 2 S96-p PPMEAsQsHLRNsPE
1 3 N100 AsQsHLRNsPEDKRA
2 11 S101-p sQsHLRNsPEDKRAG
5 23 S112-p KRAGGEEsQFEMDI_
2846 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
2855 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
3929 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated)
5123 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
7547 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (PE Conjugate)
9459 : Phospho-4E-BP1 (Thr37/46) Antibody
2846 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
2855 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
3929 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated)
4923 : Nonphospho-4E-BP1 (Thr46) (87D12) Rabbit mAb
5123 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
7547 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (PE Conjugate)
9459 : Phospho-4E-BP1 (Thr37/46) Antibody
9451 : Phospho-4E-BP1 (Ser65) Antibody
9456 : Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb
13443 : Phospho-4E-BP1 (Ser65) (D9G1Q) Rabbit mAb
9455 : Phospho-4E-BP1 (Thr70) Antibody
13396 : Phospho-4E-BP1 (Thr70) (D7F6I) Rabbit mAb
  mouse

 
S2 ______MSAGSSCSQ
S5 ___MSAGSSCSQTPS
S8 MSAGSSCSQTPSRAI
T10 AGSSCSQTPSRAIPT
S12 SSCSQTPSRAIPTRR
D24 TRRVALGDGVQLPPG
Y33-p VQLPPGDysttPGGt
S34-p QLPPGDysttPGGtL
T35-p LPPGDysttPGGtLF
T36-p PPGDysttPGGtLFs
T40-p ysttPGGtLFsttPG
S43-p tPGGtLFsttPGGtR
T44-p PGGtLFsttPGGtRI
T45-p GGtLFsttPGGtRII
T49-p FsttPGGtRIIyDRk
Y53-p PGGtRIIyDRkFLME
K56-ub tRIIyDRkFLMECRN
S64-p FLMECRNsPVAKtPP
A67 ECRNsPVAKtPPKDL
K68 CRNsPVAKtPPKDLP
T69-p RNsPVAKtPPKDLPA
A76 tPPKDLPAIPGVtsP
T81-p LPAIPGVtsPtsDEP
S82-p PAIPGVtsPtsDEPP
T84-p IPGVtsPtsDEPPMQ
S85-p PGVtsPtsDEPPMQA
S93-p DEPPMQAsQsQLPss
S95-p PPMQAsQsQLPssPE
S99-p AsQsQLPssPEDKRA
S100-p sQsQLPssPEDKRAG
S111-p KRAGGEEsQFEMDI_
2846 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
2855 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
3929 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated)
4923 : Nonphospho-4E-BP1 (Thr46) (87D12) Rabbit mAb
5123 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
7547 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (PE Conjugate)
9459 : Phospho-4E-BP1 (Thr37/46) Antibody
2846 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
2855 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
3929 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated)
4923 : Nonphospho-4E-BP1 (Thr46) (87D12) Rabbit mAb
5123 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
7547 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (PE Conjugate)
9459 : Phospho-4E-BP1 (Thr37/46) Antibody
9451 : Phospho-4E-BP1 (Ser65) Antibody
9456 : Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb
9455 : Phospho-4E-BP1 (Thr70) Antibody
  rat

 
S2 ______MSAGSSCSQ
S5 ___MSAGSSCSQTPS
S8 MSAGSSCSQTPSRAI
T10 AGSSCSQTPSRAIPT
S12 SSCSQTPSRAIPTRR
D24 TRRVALGDGVQLPPG
Y33 VQLPPGDYSttPGGT
S34 QLPPGDYSttPGGTL
T35-p LPPGDYSttPGGTLF
T36-p PPGDYSttPGGTLFS
T40 YSttPGGTLFSttPG
S43 tPGGTLFSttPGGTR
T44-p PGGTLFSttPGGTRI
T45-p GGTLFSttPGGTRII
T49 FSttPGGTRIIYDRK
Y53 PGGTRIIYDRKFLME
K56 TRIIYDRKFLMECRN
S64-p FLMECRNsPVAKtPP
A67 ECRNsPVAKtPPKDL
K68 CRNsPVAKtPPKDLP
T69-p RNsPVAKtPPKDLPT
T76 tPPKDLPTIPGVTsP
T81 LPTIPGVTsPTSDEP
S82-p PTIPGVTsPTSDEPP
T84 IPGVTsPTSDEPPMQ
S85 PGVTsPTSDEPPMQA
S93 DEPPMQASQSHLHsS
S95 PPMQASQSHLHsSPE
S99-p ASQSHLHsSPEDKRA
S100 SQSHLHsSPEDKRAG
S111-p KRAGGEEsQFEMDI_
2846 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
2855 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
3929 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated)
5123 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
7547 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (PE Conjugate)
9459 : Phospho-4E-BP1 (Thr37/46) Antibody
2846 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
2855 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
3929 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated)
5123 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
7547 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (PE Conjugate)
9459 : Phospho-4E-BP1 (Thr37/46) Antibody
9451 : Phospho-4E-BP1 (Ser65) Antibody
9456 : Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb
9455 : Phospho-4E-BP1 (Thr70) Antibody
  fruit fly

 
S2 ______MSASPTARQ
P5 ___MSASPTARQAIT
R8 MSASPTARQAITQAL
- under review  
T12 PTARQAITQALPMIT
D26 TRKVVISDPIQMPEV
Y34 PIQMPEVYSSTPGGT
S35 IQMPEVYSSTPGGTL
S36 QMPEVYSSTPGGTLY
T37 MPEVYSSTPGGTLYS
T41 YSSTPGGTLYSTTPG
S44 TPGGTLYSTTPGGTK
T45 PGGTLYSTTPGGTKL
T46 GGTLYSTTPGGTKLI
T50 YSTTPGGTKLIYERA
Y54 PGGTKLIYERAFMKN
A57 TKLIYERAFMKNLRG
S65 FMKNLRGSPLSQTPP
S68 NLRGSPLSQTPPSNV
Q69 LRGSPLSQTPPSNVP
T70 RGSPLSQTPPSNVPS
S77 TPPSNVPSCLLRGTP
G82 VPSCLLRGTPRTPFR
T83 PSCLLRGTPRTPFRK
R85 CLLRGTPRTPFRKCV
T86 LLRGTPRTPFRKCVP
- gap
- gap
- gap
- gap
- gap
2855 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
3929 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated)
2855 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
3929 : Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated)
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