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Protein Page:
ARHGEF7 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
ARHGEF7 a RAC1 guanine nucleotide exchange factor (GEF). Can induce membrane ruffling. Interacts with PAK kinases through its SH3 domain. Acts both as an activator and a downstream target for Cdc42. Its EGF-dependent phosphorylation activates its nucleotide exchange factor for Cdc42 and regulates its interaction with the E3 ligase Cbl, thus modulating Cbl-catalysed EGFR degradation. Its phosphorylation status is necessary to maintain the balance between normal signaling by EGFR and Src versus aberrant growth and transformation. Four alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: GEFs; GEFs, Rac/Rho; Motility/polarity/chemotaxis
Cellular Component: ruffle; neuron projection; protein complex; focal adhesion; cell soma; lamellipodium; cell cortex; cytosol
Molecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; guanyl-nucleotide exchange factor activity; protein kinase binding
Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; lamellipodium biogenesis; epidermal growth factor receptor signaling pathway; nervous system development; regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; signal transduction; positive regulation of GTPase activity
Reference #:  Q14155 (UniProtKB)
Alt. Names/Synonyms: ARHG7; ARHGEF7; Beta-Pix; COOL-1; COOL1; DKFZp686C12170; DKFZp761K1021; guanine nucleotide exchange factor 7; KIAA0142; KIAA0412; Nbla10314; P50; P50BP; p85; P85COOL1; P85SPR; PAK-interacting exchange factor beta; PAK3; PAK3BP; PIXB; Rho guanine nucleotide exchange factor (GEF) 7; Rho guanine nucleotide exchange factor 7; SH3 domain-containing proline-rich protein
Gene Symbols: ARHGEF7
Molecular weight: 90,012 Da
Basal Isoelectric point: 6.66  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  Microtubule Dynamics
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ARHGEF7

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
cell growth, altered: Y620‑p
cytoskeletal reorganization: S703‑p, T704‑p
activity, induced: Y620‑p, S703‑p, T704‑p
molecular association, regulation: Y620‑p, S703‑p, T704‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 K55 LLPGTIEKVYPEPRS
0 1 S123 QNFNKVLSSLVTLNK
0 15 S153-p PSSHRIKsFDSLGSQ
0 3 S156 HRIKsFDSLGSQSLH
0 9 S176-p LFQGQYRsLDMTDNS
0 3 N185 DMTDNSNNQLVVRAK
0 1 K242-u SNYVREVkAsEKPVs
0 3 S244-p YVREVkAsEKPVsPk
0 10 S249-p kAsEKPVsPksGTLK
0 1 K251-u sEKPVsPksGTLKsP
0 1 S252-p EKPVsPksGTLKsPP
0 31 S257-p PksGTLKsPPKGFDt
0 2 T264-p sPPKGFDtTAINKSY
0 1 K405 VLTTGLSKPFMRLDK
0 56 S518-p LSASPRMsGFIYQGK
0 4 T617-p DSKPAPLtPAyHTLP
2 3 Y620-p PAPLtPAyHTLPHPS
0 1 S674 LCYKEDLSKsPKTMK
0 4 S676-p YKEDLSKsPKTMKKL
3 47 S694-p RKPERKPsDEEFAsR
0 4 S700-p PsDEEFAsRKstAAL
3 13 S703-p EEFAsRKstAALEED
6 4 T704-p EFAsRKstAALEEDA
0 66 Y720-p ILKVIEAyCTSAKTR
0 3 T729 TSAKTRQTLNSTWQG
0 4 S771 RLEPSDLSEDSDYDS
0 24 - gap
0 1 - gap
0 2 - gap
0 114 - gap
0 31 - gap
  ARHGEF7 iso1  
- gap
- gap
- gap
- gap
- gap
N7 _MTDNSNNQLVVRAK
K64 SNYVREVKASEKPVS
S66 YVREVKASEKPVSPK
S71 KASEKPVSPKSGTLK
K73 SEKPVSPKSGTLKSP
S74 EKPVSPKSGTLKSPP
S79 PKSGTLKSPPKGFDT
T86 SPPKGFDTTAINKSY
K227 VLTTGLSKPFMRLDK
S340 LSASPRMSGFIYQGK
T439 DSKPAPLTPAyHTLP
Y442-p PAPLTPAyHTLPHPS
S496 LCYKEDLSKSPKTMK
S498 YKEDLSKSPKTMKKL
S516 RKPERKPSDEEFASR
S522 PSDEEFASRKstAAL
S525-p EEFASRKstAALEED
T526-p EFASRKstAALEEDA
Y542 ILKVIEAYCTSAKTR
T551-p TSAKTRQtLNSSSRK
- gap
S560-p NSSSRKEsAPQVLLP
K578 KIIVEETKsNGQTVI
S579-p IIVEETKsNGQTVIE
Y595-p KSLVDTVyALkDEVQ
K598-u VDTVyALkDEVQELR
  ARHGEF7 iso7  
K54 LLPGTIEKVYPEPRS
S101 QNFNKVLSSLVTLNK
S131 PSSHRIKSFDSLGSQ
S134 HRIKSFDSLGSQSLH
S154 LFQGQYRSLDMTDNS
N163 DMTDNSNNQLVVRAK
K220 SNYVREVKASEKPVS
S222 YVREVKASEKPVSPK
S227 KASEKPVSPKSGTLK
K229 SEKPVSPKSGTLKSP
S230 EKPVSPKSGTLKSPP
S235 PKSGTLKSPPKGFDT
T242 SPPKGFDTTAINKSY
K383 VLTTGLSKPFMRLDK
S496 LSASPRMSGFIYQGK
T595 DSKPAPLTPAYHTLP
Y598 PAPLTPAYHTLPHPS
S652 LCYKEDLSKSPKTMK
S654 YKEDLSKSPKTMKKL
S672-p RKPERKPsMRSSRPG
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  mouse

► Hide Isoforms
 
K55-u LLPGTIEkVYPEPRN
S102-p QNFNKVLsSLVTLNK
S132-p PSSHRIKsFDsLGSQ
S135-p HRIKsFDsLGSQSSH
S155-p LLQSQYRsLDMTDNT
S164-p DMTDNTNsQLVVRAK
K221 SNYVREIKPsEKPVs
S223-p YVREIKPsEKPVsPK
S228-p KPsEKPVsPKSGTLK
K230 sEKPVsPKSGTLKsP
S231 EKPVsPKSGTLKsPP
S236-p PKSGTLKsPPKGFDt
T243-p sPPKGFDtTAINKSY
K384-u VLTTGLSkPFMRLDK
S497-p LSASPRMsGFIYQGK
T596 DSKPVALTPAYHTLP
Y599 PVALTPAYHTLPHPS
S653-p LCYKEDLsKsPKTMK
S655-p YKEDLsKsPKTMKKL
S673-p RKPERKPsDEEFAVR
V679 PsDEEFAVRKstAAL
S682-p EEFAVRKstAALEED
T683-p EFAVRKstAALEEDA
Y699 ILKVIEAYCTSAKTR
T708 TSAKTRQTLNSTWQG
S750-p RLEPSDLsEDSEYDS
S776-p ASRSRKEsAPQVLLP
K794-u KIIVEETksNGQTVI
S795-p IIVEETksNGQTVIE
Y811-p KSLVDTVyALkDEVQ
K814-u VDTVyALkDEVQELR
  ARHGEF7 iso2  
K55 LLPGTIEKVYPEPRN
S102 QNFNKVLSSLVTLNK
S132 PSSHRIKSFDSLGSQ
S135 HRIKSFDSLGSQSSH
S155 LLQSQYRSLDMTDNT
S164 DMTDNTNSQLVVRAK
K221 SNYVREIKPSEKPVS
S223 YVREIKPSEKPVSPK
S228 KPSEKPVSPKSGTLK
K230 SEKPVSPKSGTLKSP
S231 EKPVSPKSGTLKSPP
S236 PKSGTLKSPPKGFDT
T243 SPPKGFDTTAINKSY
K384 VLTTGLSKPFMRLDK
S497 LSASPRMSGFIYQGK
T596 DSKPVALTPAYHTLP
Y599 PVALTPAYHTLPHPS
S653 LCYKEDLSKSPKTMK
S655 YKEDLSKSPKTMKKL
S673 RKPERKPSDEEFAVR
V679 PSDEEFAVRKSTAAL
S682 EEFAVRKSTAALEED
T683 EFAVRKSTAALEEDA
Y699 ILKVIEAYCTSAKTR
T708 TSAKTRQTLNSSSRK
- gap
S717 NSSSRKESAPQVLLP
K735 KIIVEETKSNGQTVI
S736 IIVEETKSNGQTVIE
Y752 KSLVDTVYALKDEVQ
K755 VDTVYALKDEVQELR
  ARHGEF7 iso3  
K55 LLPGTIEKVYPEPRN
S102 QNFNKVLSSLVTLNK
S132 PSSHRIKSFDSLGSQ
S135 HRIKSFDSLGSQSSH
S155 LLQSQYRSLDMTDNT
S164 DMTDNTNSQLVVRAK
K221 SNYVREIKPSEKPVS
S223 YVREIKPSEKPVSPK
S228 KPSEKPVSPKSGTLK
K230 SEKPVSPKSGTLKSP
S231 EKPVSPKSGTLKSPP
S236 PKSGTLKSPPKGFDT
T243 SPPKGFDTTAINKSY
K384 VLTTGLSKPFMRLDK
S497 LSASPRMSGFIYQGK
- gap
- gap
S578 VPSHTDLSKsPKTMK
S580-p SHTDLSKsPKTMKKL
S598 RKPERKPSDEEFAVR
V604 PSDEEFAVRKSTAAL
S607 EEFAVRKSTAALEED
T608 EFAVRKSTAALEEDA
Y624 ILKVIEAYCTSAKTR
T633 TSAKTRQTLNSTWQG
S675 RLEPSDLSEDSEYDS
S701 ASRSRKESAPQVLLP
K719 KIIVEETKSNGQTVI
S720 IIVEETKSNGQTVIE
Y736-p KSLVDTVyALKDEVQ
K739 VDTVyALKDEVQELR
  rat

 
- gap
- gap
- gap
- gap
- gap
S7 _MTDNANSQLVVRAK
K64 SNYVREIKPSEKPVS
S66 YVREIKPSEKPVSPK
S71 KPSEKPVSPKSGTLK
K73 SEKPVSPKSGTLKSP
S74 EKPVSPKSGTLKSPP
S79 PKSGTLKSPPKGFDT
T86 SPPKGFDTTAINKSY
K227 VLTTGLSKPFMRLDK
S340-p LSASPRMsGFIYQGK
T439 DSKPVALTPAYHTLP
Y442 PVALTPAYHTLPHPS
S496 LCYKEDLSRSPKTMK
S498 YKEDLSRSPKTMKKL
S516-p RKPERKPsDEEFAVR
V522 PsDEEFAVRKStAAL
S525 EEFAVRKStAALEED
T526-p EFAVRKStAALEEDA
Y542 ILKVIEAYCTSAKTR
T551 TSAKTRQTLNSSSRK
- gap
S560-p NSSSRKEsAPQVLLP
K578 KIIVEETKSNGQTVI
S579 IIVEETKSNGQTVIE
Y595 KSLVDTVYALKDEVQ
K598 VDTVYALKDEVQELR
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