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Protein Page:
ARHGEF7 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ARHGEF7 a RAC1 guanine nucleotide exchange factor (GEF). Can induce membrane ruffling. Interacts with PAK kinases through its SH3 domain. Acts both as an activator and a downstream target for Cdc42. Its EGF-dependent phosphorylation activates its nucleotide exchange factor for Cdc42 and regulates its interaction with the E3 ligase Cbl, thus modulating Cbl-catalysed EGFR degradation. Its phosphorylation status is necessary to maintain the balance between normal signaling by EGFR and Src versus aberrant growth and transformation. Four alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: GEFs, Rac/Rho; GEFs; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 13q34
Cellular Component: ruffle; neuron projection; protein complex; focal adhesion; cell soma; lamellipodium; cell cortex; cytosol
Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; protein kinase binding
Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; lamellipodium biogenesis; epidermal growth factor receptor signaling pathway; nervous system development; regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; signal transduction; positive regulation of Rho GTPase activity; positive regulation of GTPase activity
Reference #:  Q14155 (UniProtKB)
Alt. Names/Synonyms: ARHG7; ARHGEF7; Beta-Pix; COOL-1; COOL1; DKFZp686C12170; DKFZp761K1021; guanine nucleotide exchange factor 7; KIAA0142; KIAA0412; Nbla10314; P50; P50BP; p85; P85COOL1; P85SPR; PAK-interacting exchange factor beta; PAK3; PAK3BP; PIXB; Rho guanine nucleotide exchange factor (GEF) 7; Rho guanine nucleotide exchange factor 7; SH3 domain-containing proline-rich protein
Gene Symbols: ARHGEF7
Molecular weight: 90,012 Da
Basal Isoelectric point: 6.66  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  Microtubule Dynamics
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ARHGEF7

Protein Structure Not Found.


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Sites Implicated In
cell growth, altered: Y620‑p
cytoskeletal reorganization: S703‑p, T704‑p
exocytosis, induced: S518‑p, S761‑p
activity, induced: Y620‑p, S703‑p, T704‑p
molecular association, regulation: Y620‑p, S703‑p, T704‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 K55 LLPGTIEKVYPEPRS
0 1 Y113 TFDANDLYQGQNFNK
0 1 S123 QNFNKVLSSLVTLNK
0 15 S153-p PSSHRIKsFDSLGSQ
0 3 S156 HRIKsFDSLGSQSLH
0 10 S176-p LFQGQYRsLDMTDNS
0 1 S183 sLDMTDNSNNQLVVR
0 4 N185 DMTDNSNNQLVVRAK
0 1 K242-ub SNYVREVkAsEKPVs
0 4 S244-p YVREVkAsEKPVsPk
0 14 S249-p kAsEKPVsPksGtLK
0 1 K251-ub sEKPVsPksGtLKsP
0 1 S252-p EKPVsPksGtLKsPP
0 3 T254-p PVsPksGtLKsPPKG
0 37 S257-p PksGtLKsPPKGFDt
0 3 T264-p sPPKGFDtTAINKSY
0 1 T265 PPKGFDtTAINKSYY
0 1 S295 KELQTVLSTYLRPLQ
0 1 K405 VLTTGLSKPFMRLDK
0 1 Y427-p LERHMEDyHTDRQDI
0 1 K455 CQEVRKRKELELQIL
2 68 S518-p LSASPRMsGFIYQGK
0 1 Y522 PRMsGFIYQGKLPtT
0 1 T528-p IYQGKLPtTGMTITK
0 1 S539-p TITKLEDsENHRNAF
0 1 T580-p LQKQTKVtsVGNPTI
0 1 S581-p QKQTKVtsVGNPTIK
0 1 S599-p VPSHTLPsHPVtPSS
0 1 T603-p TLPsHPVtPSSKHAD
0 5 T617-p DSKPAPLtPAyHTLP
2 4 Y620-p PAPLtPAyHTLPHPS
0 1 S674 LCYKEDLSksPkTMk
0 1 K675-ac CYKEDLSksPkTMkk
0 6 S676-p YKEDLSksPkTMkkL
0 1 K678-ac EDLSksPkTMkkLLP
0 1 K681-ac SksPkTMkkLLPKRK
0 1 K682-ac ksPkTMkkLLPKRKP
3 61 S694-p RKPERKPsDEEFAsR
0 5 S700-p PsDEEFAsRKstAAL
3 14 S703-p EEFAsRKstAALEED
7 6 T704-p EFAsRKstAALEEDA
0 1 K715-ub EEDAQILkVIEAyCt
0 70 Y720-p ILkVIEAyCtsAKTR
0 1 T722-p kVIEAyCtsAKTRQT
0 1 S723-p VIEAyCtsAKTRQTL
0 3 T729 tsAKTRQTLNSTWQG
1 1 S761-p DSLGRRSsLSRLEPS
0 4 S771 RLEPSDLSEDSDYDS
0 1 S784-p DSIWTAHsyRMGSTS
0 1 Y785-p SIWTAHsyRMGSTSR
0 38 - gap
0 1 - gap
0 2 - gap
0 1 - gap
0 113 - gap
0 31 - gap
0 1 S800-p KSCCSYIsHQN____
  ARHGEF7 iso1  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
N7 _MTDNSNNQLVVRAK
K64 SNYVREVKASEKPVS
S66 YVREVKASEKPVSPK
S71 KASEKPVSPKSGTLK
K73 SEKPVSPKSGTLKSP
S74 EKPVSPKSGTLKSPP
T76 PVSPKSGTLKSPPKG
S79 PKSGTLKSPPKGFDT
T86 SPPKGFDTTAINKSY
T87 PPKGFDTTAINKSYY
S117 KELQTVLSTYLRPLQ
K227 VLTTGLSKPFMRLDK
Y249 LERHMEDYHTDRQDI
K277 CQEVRKRKELELQIL
S340-p LSASPRMsGFIYQGK
Y344 PRMsGFIYQGKLPTT
T350 IYQGKLPTTGMTITK
S361 TITKLEDSENHRNAF
T402 LQKQTKVTSVGNPTI
S403 QKQTKVTSVGNPTIK
S421 VPSHTLPSHPVTPSS
T425 TLPSHPVTPSSKHAD
T439 DSKPAPLTPAyHTLP
Y442-p PAPLTPAyHTLPHPS
S496 LCYKEDLSKSPKTMK
K497 CYKEDLSKSPKTMKK
S498 YKEDLSKSPKTMKKL
K500 EDLSKSPKTMKKLLP
K503 SKSPKTMKKLLPKRK
K504 KSPKTMKKLLPKRKP
S516 RKPERKPSDEEFASR
S522 PSDEEFASRKstAAL
S525-p EEFASRKstAALEED
T526-p EFASRKstAALEEDA
K537 EEDAQILKVIEAYCT
Y542 ILKVIEAYCTSAKTR
T544 KVIEAYCTSAKTRQt
S545 VIEAYCTSAKTRQtL
T551-p TSAKTRQtLNSSSRK
- gap
- gap
- gap
- gap
S560-p NSSSRKEsAPQVLLP
K578 KIIVEETKsNGQTVI
S579-p IIVEETKsNGQTVIE
K588-ub GQTVIEEkSLVDTVy
Y595-p kSLVDTVyALkDEVQ
K598-ub VDTVyALkDEVQELR
- gap
  ARHGEF7 iso7  
K54 LLPGTIEKVYPEPRS
Y91 TFDANDLYQGQNFNK
S101 QNFNKVLSSLVTLNK
S131 PSSHRIKSFDSLGSQ
S134 HRIKSFDSLGSQSLH
S154 LFQGQYRSLDMTDNS
S161 SLDMTDNSNNQLVVR
N163 DMTDNSNNQLVVRAK
K220 SNYVREVKASEKPVS
S222 YVREVKASEKPVSPK
S227 KASEKPVSPKSGTLK
K229 SEKPVSPKSGTLKSP
S230 EKPVSPKSGTLKSPP
T232 PVSPKSGTLKSPPKG
S235 PKSGTLKSPPKGFDT
T242 SPPKGFDTTAINKSY
T243 PPKGFDTTAINKSYY
S273 KELQTVLSTYLRPLQ
K383 VLTTGLSKPFMRLDK
Y405 LERHMEDYHTDRQDI
K433 CQEVRKRKELELQIL
S496 LSASPRMSGFIYQGK
Y500 PRMSGFIYQGKLPTT
T506 IYQGKLPTTGMTITK
S517 TITKLEDSENHRNAF
T558 LQKQTKVTSVGNPTI
S559 QKQTKVTSVGNPTIK
S577 VPSHTLPSHPVTPSS
T581 TLPSHPVTPSSKHAD
T595 DSKPAPLTPAYHTLP
Y598 PAPLTPAYHTLPHPS
S652 LCYKEDLSKSPKTMK
K653 CYKEDLSKSPKTMKK
S654 YKEDLSKSPKTMKKL
K656 EDLSKSPKTMKKLLP
K659 SKSPKTMKKLLPKRK
K660 KSPKTMKKLLPKRKP
S672-p RKPERKPsMRSSRPG
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  mouse

► Hide Isoforms
 
K55-ub LLPGTIEkVYPEPRN
Y92-p TFDANDLyQGQNFNK
S102-p QNFNKVLsSLVTLNK
S132-p PSSHRIKsFDsLGSQ
S135-p HRIKsFDsLGSQSSH
S155-p LLQSQYRsLDMTDNt
T162-p sLDMTDNtNsQLVVR
S164-p DMTDNtNsQLVVRAK
K221 SNYVREIKPsEKPVs
S223-p YVREIKPsEKPVsPK
S228-p KPsEKPVsPKSGtLK
K230 sEKPVsPKSGtLKsP
S231 EKPVsPKSGtLKsPP
T233-p PVsPKSGtLKsPPKG
S236-p PKSGtLKsPPKGFDt
T243-p sPPKGFDttAINKSY
T244-p PPKGFDttAINKSYY
S274-p KELQSVLsTYLRPLQ
K384-ub VLTTGLSkPFMRLDK
Y406 LERHMEDYHPDRQDI
K434-ac CQEVRKRkELELQIL
S497-p LSASPRMsGFIyQGK
Y501-p PRMsGFIyQGKLPTT
T507 IyQGKLPTTGMTITK
S518 TITKLEDSENHRNAF
T559 LQKQTKVTSVSNPTI
S560 QKQTKVTSVSNPTIK
S578 VPSHTLPSHPLTPSS
T582 TLPSHPLTPSSKHAD
T596 DSKPVALTPAYHTLP
Y599 PVALTPAYHTLPHPS
S653-p LCYKEDLsKsPKTMK
K654 CYKEDLsKsPKTMKK
S655-p YKEDLsKsPKTMKKL
K657 EDLsKsPKTMKKLLP
K660 sKsPKTMKKLLPKRK
K661 KsPKTMKKLLPKRKP
S673-p RKPERKPsDEEFAVR
V679 PsDEEFAVRKstAAL
S682-p EEFAVRKstAALEED
T683-p EFAVRKstAALEEDA
K694 EEDAQILKVIEAYCT
Y699 ILKVIEAYCTSAKTR
T701 KVIEAYCTSAKTRQT
S702 VIEAYCTSAKTRQTL
T708 TSAKTRQTLNSTWQG
S740 DSLGRRSSLSRLEPS
S750-p RLEPSDLsEDSEYDS
S763 DSIWTAHSYRMGSAS
Y764 SIWTAHSYRMGSASR
S776-p ASRSRKEsAPQVLLP
K794-ub KIIVEETksNGQTVI
S795-p IIVEETksNGQTVIE
K804 GQTVIEEKSLVDTVy
Y811-p KSLVDTVyALkDEVQ
K814-ub VDTVyALkDEVQELR
- gap
  ARHGEF7 iso2  
K55 LLPGTIEKVYPEPRN
Y92 TFDANDLYQGQNFNK
S102 QNFNKVLSSLVTLNK
S132 PSSHRIKSFDSLGSQ
S135 HRIKSFDSLGSQSSH
S155 LLQSQYRSLDMTDNT
T162 SLDMTDNTNSQLVVR
S164 DMTDNTNSQLVVRAK
K221 SNYVREIKPSEKPVS
S223 YVREIKPSEKPVSPK
S228 KPSEKPVSPKSGTLK
K230 SEKPVSPKSGTLKSP
S231 EKPVSPKSGTLKSPP
T233 PVSPKSGTLKSPPKG
S236 PKSGTLKSPPKGFDT
T243 SPPKGFDTTAINKSY
T244 PPKGFDTTAINKSYY
S274 KELQSVLSTYLRPLQ
K384 VLTTGLSKPFMRLDK
Y406 LERHMEDYHPDRQDI
K434 CQEVRKRKELELQIL
S497 LSASPRMSGFIYQGK
Y501 PRMSGFIYQGKLPTT
T507 IYQGKLPTTGMTITK
S518 TITKLEDSENHRNAF
T559 LQKQTKVTSVSNPTI
S560 QKQTKVTSVSNPTIK
S578 VPSHTLPSHPLTPSS
T582 TLPSHPLTPSSKHAD
T596 DSKPVALTPAYHTLP
Y599 PVALTPAYHTLPHPS
S653 LCYKEDLSKSPKTMK
K654 CYKEDLSKSPKTMKK
S655 YKEDLSKSPKTMKKL
K657 EDLSKSPKTMKKLLP
K660 SKSPKTMKKLLPKRK
K661 KSPKTMKKLLPKRKP
S673 RKPERKPSDEEFAVR
V679 PSDEEFAVRKSTAAL
S682 EEFAVRKSTAALEED
T683 EFAVRKSTAALEEDA
K694 EEDAQILKVIEAYCT
Y699 ILKVIEAYCTSAKTR
T701 KVIEAYCTSAKTRQT
S702 VIEAYCTSAKTRQTL
T708 TSAKTRQTLNSSSRK
- gap
- gap
- gap
- gap
S717 NSSSRKESAPQVLLP
K735 KIIVEETKSNGQTVI
S736 IIVEETKSNGQTVIE
K745 GQTVIEEKSLVDTVY
Y752 KSLVDTVYALKDEVQ
K755 VDTVYALKDEVQELR
- gap
  ARHGEF7 iso3  
K55 LLPGTIEKVYPEPRN
Y92 TFDANDLYQGQNFNK
S102 QNFNKVLSSLVTLNK
S132 PSSHRIKSFDSLGSQ
S135 HRIKSFDSLGSQSSH
S155 LLQSQYRSLDMTDNT
T162 SLDMTDNTNSQLVVR
S164 DMTDNTNSQLVVRAK
K221 SNYVREIKPSEKPVS
S223 YVREIKPSEKPVSPK
S228 KPSEKPVSPKSGTLK
K230 SEKPVSPKSGTLKSP
S231 EKPVSPKSGTLKSPP
T233 PVSPKSGTLKSPPKG
S236 PKSGTLKSPPKGFDT
T243 SPPKGFDTTAINKSY
T244 PPKGFDTTAINKSYY
S274 KELQSVLSTYLRPLQ
K384 VLTTGLSKPFMRLDK
Y406 LERHMEDYHPDRQDI
K434 CQEVRKRKELELQIL
S497 LSASPRMSGFIYQGK
Y501 PRMSGFIYQGKLPTT
T507 IYQGKLPTTGMTITK
S518 TITKLEDSENHRNAF
T559 LQKQTKVTSVSNPTI
S560 QKQTKVTSVSNPTIK
- gap
- gap
- gap
- gap
S578 VPSHTDLSKsPKTMK
K579 PSHTDLSKsPKTMKK
S580-p SHTDLSKsPKTMKKL
K582 TDLSKsPKTMKKLLP
K585 SKsPKTMKKLLPKRK
K586 KsPKTMKKLLPKRKP
S598 RKPERKPSDEEFAVR
V604 PSDEEFAVRKSTAAL
S607 EEFAVRKSTAALEED
T608 EFAVRKSTAALEEDA
K619 EEDAQILKVIEAYCT
Y624 ILKVIEAYCTSAKTR
T626 KVIEAYCTSAKTRQT
S627 VIEAYCTSAKTRQTL
T633 TSAKTRQTLNSTWQG
S665 DSLGRRSSLSRLEPS
S675 RLEPSDLSEDSEYDS
S688 DSIWTAHSYRMGSAS
Y689 SIWTAHSYRMGSASR
S701 ASRSRKESAPQVLLP
K719 KIIVEETKSNGQTVI
S720 IIVEETKSNGQTVIE
K729 GQTVIEEKSLVDTVY
Y736 KSLVDTVYALKDEVQ
K739 VDTVYALKDEVQELR
- gap
  rat

 
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S7-p _MTDNANsQLVVRAK
K64 SNYVREIKPSEKPVs
S66 YVREIKPSEKPVsPK
S71-p KPSEKPVsPKSGTLK
K73 SEKPVsPKSGTLKsP
S74 EKPVsPKSGTLKsPP
T76 PVsPKSGTLKsPPKG
S79-p PKSGTLKsPPKGFDT
T86 sPPKGFDTTAINKSY
T87 PPKGFDTTAINKSYY
S117 KELQSVLSTYLWPLQ
K227 VLTTGLSKPFMRLDK
Y249 LERHMEDYHPDRQDI
K277 CQEVRKRKELELQIL
S340-p LSASPRMsGFIYQGK
Y344 PRMsGFIYQGKLPTT
T350 IYQGKLPTTGMTITK
S361 TITKLEDSENHRNAF
T402 LQRQTKVTSVSNPTI
S403 QRQTKVTSVSNPTIK
S421 VPSHTLPSHPLTPSS
T425 TLPSHPLTPSSKHAD
T439 DSKPVALTPAYHTLP
Y442 PVALTPAYHTLPHPS
S496 LCYKEDLSRsPKTMK
R497 CYKEDLSRsPKTMKK
S498-p YKEDLSRsPKTMKKL
K500 EDLSRsPKTMKKLLP
K503 SRsPKTMKKLLPKRK
K504 RsPKTMKKLLPKRKP
S516-p RKPERKPsDEEFAVR
V522 PsDEEFAVRKStAAL
S525 EEFAVRKStAALEED
T526-p EFAVRKStAALEEDA
K537 EEDAQILKVIEAYCT
Y542 ILKVIEAYCTSAKTR
T544 KVIEAYCTSAKTRQT
S545 VIEAYCTSAKTRQTL
T551 TSAKTRQTLNSSSRK
- gap
- gap
- gap
- gap
S560-p NSSSRKEsAPQVLLP
K578 KIIVEETKSNGQTVI
S579 IIVEETKSNGQTVIE
K588 GQTVIEEKSLVDTVY
Y595 KSLVDTVYALKDEVQ
K598 VDTVYALKDEVQELR
- gap
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