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Protein Page:
Smad2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Smad2 ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated. Two alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor
Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; nucleus; cytosol
Molecular Function: transcription activator binding; metal ion binding; transcription factor binding; phosphatase binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; chromatin binding; SMAD binding; transcription factor activity; transforming growth factor beta receptor binding
Biological Process: developmental growth; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; gastrulation; primary microRNA processing; palate development; negative regulation of transcription from RNA polymerase II promoter; post-embryonic development; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; ureteric bud development; transforming growth factor beta receptor signaling pathway; pancreas development; embryonic foregut morphogenesis; response to glucose stimulus; positive regulation of BMP signaling pathway; pericardium development; transcription initiation from RNA polymerase II promoter; cell fate commitment; regulation of binding; transcription, DNA-dependent; in utero embryonic development; common-partner SMAD protein phosphorylation; embryonic cranial skeleton morphogenesis; SMAD protein complex assembly; organ growth; zygotic determination of dorsal/ventral axis; mesoderm formation; endoderm formation; insulin secretion; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; lung development
Reference #:  Q15796 (UniProtKB)
Alt. Names/Synonyms: hMAD-2; hSMAD2; JV18; JV18-1; MAD homolog 2; Mad protein homolog; MAD, mothers against decapentaplegic homolog 2; Mad-related protein 2; MADH2; MADR2; MGC22139; MGC34440; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; SMAD2
Gene Symbols: SMAD2
Molecular weight: 52,306 Da
Basal Isoelectric point: 6.13  Predict pI for various phosphorylation states
CST Pathways:  Angiogenesis  |  ESC Pluripotency and Differentiation  |  TGF-├č Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Smad2

Protein Structure Not Found.


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Sites Implicated In
cell motility, altered: S245‑p, S250‑p, S255‑p
transcription, altered: S464‑p, S465‑p, S467‑p
transcription, induced: T8‑p, T220‑p, S245‑p, S250‑p, S255‑p, S464‑p, S465‑p, S467‑p
activity, induced: T8‑p, T220‑p, S245‑p, S250‑p, S255‑p, S464‑p, S465‑p, S467‑p
intracellular localization: S110‑p, T220‑p, S240‑p, S245‑p, S250‑p, S255‑p, S260‑p, S464‑p, S465‑p, S467‑p
molecular association, regulation: T8‑p, S110‑p, T220‑p, S240‑p, S245‑p, S250‑p, S255‑p, S260‑p, S465‑p, S467‑p
protein conformation: S245‑p, S250‑p, S255‑p
protein stabilization: T8‑p, T220‑p, S245‑p, S250‑p, S255‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S2-p ______MsSILPFtP
3 11 T8-p MsSILPFtPPVVKRL
2 4 K19-ac VKRLLGWkkSAGGSG
1 0 K20-ac KRLLGWkkSAGGSGG
1 0 K39-ac EQNGQEEkWCEKAVK
0 12 Y102-p QWDTTGLySFSEQTR
1 0 S110-p SFSEQTRsLDGRLQV
0 1 K157-ub EYAFNLKkDEVCVNP
0 3 T172-p YHYQRVEtPVLPPVL
1 0 T197-p LPPLDDYtHSIPENT
6 0 T220-p QSNYIPEtPPPGYIS
2 0 S240-p SDQQLNQsMDTGsPA
5 0 S245-p NQsMDTGsPAELsPT
3 0 S250-p TGsPAELsPTTLsPV
4 0 S255-p ELsPTTLsPVNHsLD
1 0 S260-p TLsPVNHsLDLQPVT
0 1 T324-p SNVNRNAtVEMTRRH
1 0 S417-p RMCTIRMsFVKGWGA
1 0 S433 YRRQTVTSTPCWIEL
0 6 S458-p KVLTQMGsPsVRCss
0 2 S460-p LTQMGsPsVRCssMs
5 1 S464-p GsPsVRCssMs____
31 5 S465-p sPsVRCssMs_____
31 5 S467-p sVRCssMs_______
3104 : Phospho-Smad2 (Ser245/250/255) Antibody
3104 : Phospho-Smad2 (Ser245/250/255) Antibody
3104 : Phospho-Smad2 (Ser245/250/255) Antibody
3101 : Phospho-Smad2 (Ser465/467) Antibody
3108 : Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
11979 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (PE Conjugate)
3101 : Phospho-Smad2 (Ser465/467) Antibody
3108 : Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
11979 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (PE Conjugate)
  mouse

 
S2 ______MSSILPFtP
T8-p MSSILPFtPPVVKRL
K19 VKRLLGWKKSAGGSG
K20 KRLLGWKKSAGGSGG
K39 EQNGQEEKWCEKAVK
Y102-p QWDTTGLySFSEQTR
S110 SFSEQTRSLDGRLQV
K157 EYAFNLKKDEVCVNP
T172-p YHYQRVEtPVLPPVL
T197 LPPLDDYTHSIPENT
T220 QSNYIPETPPPGYIS
S240 SDQQLNQSMDTGSPA
S245 NQSMDTGSPAELSPT
S250 TGSPAELSPTTLSPV
S255 ELSPTTLSPVNHSLD
S260 TLSPVNHSLDLQPVT
T324 SNVNRNATVEMTRRH
S417 RMCTIRMSFVKGWGA
S433-p YRRQTVTsTPCWIEL
S458 KVLTQMGSPSVRCSs
S460 LTQMGSPSVRCSsMs
S464 GSPSVRCSsMs____
S465-p SPSVRCSsMs_____
S467-p SVRCSsMs_______
3104 : Phospho-Smad2 (Ser245/250/255) Antibody
3104 : Phospho-Smad2 (Ser245/250/255) Antibody
3104 : Phospho-Smad2 (Ser245/250/255) Antibody
3101 : Phospho-Smad2 (Ser465/467) Antibody
3108 : Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
11979 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (PE Conjugate)
3101 : Phospho-Smad2 (Ser465/467) Antibody
3108 : Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
11979 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (PE Conjugate)
  rat

 
S2 ______MSSILPFTP
T8 MSSILPFTPPVVKRL
K19 VKRLLGWKKSAGGSG
K20 KRLLGWKKSAGGSGG
K39 EQNGQEEKWCEKAVK
Y102 QWDTTGLYSFSEQTR
S110 SFSEQTRSLDGRLQV
K157 EYAFSLKKDEVCVNP
T172 YHYQRVETPVLPPVL
T197 LPPLDDYTHSIPENT
T220 QSNYIPETPPPGYIS
S240 SDQQLNQSMDTGSPA
S245 NQSMDTGSPAELSPT
S250 TGSPAELSPTTLSPV
S255 ELSPTTLSPVNHSLD
S260 TLSPVNHSLDLQPVT
T324 SNVNRNATVEMTRRH
S417 RMCTIRMSFVKGWGA
S433 YRRQTVTSTPCWIEL
S458 KVLTQMGSPSVRCSs
S460 LTQMGSPSVRCSsMs
S464 GSPSVRCSsMs____
S465-p SPSVRCSsMs_____
S467-p SVRCSsMs_______
3104 : Phospho-Smad2 (Ser245/250/255) Antibody
3104 : Phospho-Smad2 (Ser245/250/255) Antibody
3104 : Phospho-Smad2 (Ser245/250/255) Antibody
3101 : Phospho-Smad2 (Ser465/467) Antibody
3108 : Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
11979 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (PE Conjugate)
3101 : Phospho-Smad2 (Ser465/467) Antibody
3108 : Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
11979 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (PE Conjugate)
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