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Protein Page:
HS1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
HS1 Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3. Interacts with FES/FPS. Interacts (via SH2 domain) with FGR. Forms a multiprotein complex with LYN and ANKRD54. Expressed only in tissues and cells of hematopoietic origin. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold
Cellular Component: transcription factor complex; mitochondrion; membrane; cytoplasm; nucleus
Molecular Function: protein binding; protein complex binding; actin binding; protein kinase binding; SH3 domain binding
Biological Process: positive regulation of granulocyte differentiation; response to hormone stimulus; negative regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; regulation of actin filament polymerization; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of STAT protein; regulation of transcription, DNA-dependent; actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of macrophage differentiation
Reference #:  P14317 (UniProtKB)
Alt. Names/Synonyms: cortactin-like; CTTNL; HCLS1; Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; HS1; LckBP1; p75
Gene Symbols: HCLS1
Molecular weight: 54,014 Da
Basal Isoelectric point: 4.74  Predict pI for various phosphorylation states
CST Pathways:  B Cell Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

HS1

Protein Structure Not Found.


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Sites Implicated In
apoptosis, altered: Y378‑p, Y397‑p
cell adhesion, altered: Y397‑p
cell motility, altered: Y378‑p
cytoskeletal reorganization: Y378‑p, Y397‑p
transcription, altered: Y378‑p, Y397‑p
intracellular localization: Y378‑p, Y397‑p
molecular association, regulation: Y378‑p, Y397‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S13-p VGHDVSVsVEtQGDD
1 4 T16-p DVSVsVEtQGDDWDt
1 13 T23-p tQGDDWDtDPDFVND
0 2 S32-p PDFVNDIsEkEQRWG
0 1 K34-ub FVNDIsEkEQRWGAk
0 1 K41-ac kEQRWGAktIEGSGR
0 11 T42-p EQRWGAktIEGSGRT
0 1 K60-ub NIHQLRNkVSEEHDV
0 6 Y83-p GPKASHGyGGRFGVE
0 1 S97-p ERDRMDKsAVGHEyV
0 134 Y103-p KsAVGHEyVAEVEKH
0 1 K123-ac AAKGFGGkyGVERDR
0 30 Y124-p AKGFGGkyGVERDRA
0 3 S134-p ERDRADKsAVGFDyk
0 97 Y140-p KsAVGFDykGEVEKH
0 1 K141-ac sAVGFDykGEVEKHT
0 12 Y153-p KHTSQKDysRGFGGR
0 8 S154-p HTSQKDysRGFGGRy
0 4 Y161-p sRGFGGRyGVEKDKW
0 150 Y175-p WDKAALGyDyKGEtE
0 112 Y177-p KAALGyDyKGEtEKH
0 1 T181-p GyDyKGEtEKHEsQR
0 1 S186-p GEtEKHEsQRDyAkG
0 1 D189 EKHEsQRDyAkGFGG
0 47 Y190-p KHEsQRDyAkGFGGQ
0 1 K192-ac EsQRDyAkGFGGQyG
0 265 Y198-p AkGFGGQyGIQKDRV
0 2 S208-p QKDRVDKsAVGFNEM
0 1 T219-p FNEMEAPttAykkTT
0 1 T220-p NEMEAPttAykkTTP
3 144 Y222-p MEAPttAykkTTPIE
0 2 K223-ac EAPttAykkTTPIEA
0 1 K224-ac APttAykkTTPIEAA
0 1 K239-ub SSGTRGLkAkFESMA
0 1 K241-ac GTRGLkAkFESMAEE
0 33 S275-p RKAVTKRsPEAPQPV
0 5 T308-p EAWPPVGtPPSSESE
0 5 H323 PVRTSREHPVPLLPI
0 1 L327 SREHPVPLLPIRQtL
0 1 I330 HPVPLLPIRQtLPED
0 6 T333-p PLLPIRQtLPEDNEE
0 18 Y360-p QVEEEPVyEAEPEPE
0 1 P374 EPEPEPEPENDyEDV
7 38 Y378-p EPEPENDyEDVEEMD
8 8 Y397-p EDEPEGDyEEVLEPE
0 4 Y481-p FGLFPANyVKLLE__
4507 : Phospho-HS1 (Tyr397) Antibody
8714 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb
11880 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb (PE Conjugate)
13090 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
13708 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
13742 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb (Pacific Blue(TM) Conjugate)
  mouse

 
S13 VGHDVSVSVETQGDD
T16 DVSVSVETQGDDWDT
T23 TQGDDWDTDPDFVND
S32 PDFVNDISEKEQRWG
K34 FVNDISEKEQRWGAK
K41 KEQRWGAKTIEGSGR
T42 EQRWGAKTIEGSGRT
K60 NIHQLRNKVSEEHDI
Y83 GPKASHGYGGRFGVE
S97 ERDRMDKSAVGHEyV
Y103-p KSAVGHEyVADVEKH
K123 AARGFGGKYGVERDR
Y124 ARGFGGKYGVERDRA
S134 ERDRADKSAVGFDyK
Y140-p KSAVGFDyKGEVEKH
K141 SAVGFDyKGEVEKHA
Y153-p KHASQKDySHGFGGR
S154 HASQKDySHGFGGRY
Y161 SHGFGGRYGVEKDKR
Y175-p RDKAALGyDyKGETE
Y177-p KAALGyDyKGETEKH
T181 GyDyKGETEKHESQR
S186 GETEKHESQRdYAKG
D189-ca EKHESQRdYAKGFGG
Y190 KHESQRdYAKGFGGQ
K192 ESQRdYAKGFGGQyG
Y198-p AKGFGGQyGIQKDRV
S208 QKDRVDKSAVGFNEM
T219 FNEMEAPTTAyKKTT
T220 NEMEAPTTAyKKTTP
Y222-p MEAPTTAyKKTTPIE
K223 EAPTTAyKKTTPIEA
K224 APTTAyKKTTPIEAA
K239 SSGARGLKAKFESLA
K241 GARGLKAKFESLAEE
S275 RKAVVKMSREVQQPS
S308 EVWPPAESHLPPESQ
Y323-p PVRSRREyPVPsLPt
S327-p RREyPVPsLPtRQsP
T330-p yPVPsLPtRQsPLQN
S333-p PsLPtRQsPLQNHLE
Y364-p QVVEEPVyEAAPELE
T384-p DYEPEPEtEPDyEDV
Y388-p EPEtEPDyEDVGELD
Y405-p DEDAEGDyEDVLEPE
Y482 FGLFPANYVKLL___
8714 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb
11880 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb (PE Conjugate)
13708 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
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