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Protein Page:
HS1 (mouse)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
HS1 Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3. Interacts with FES/FPS. Interacts (via SH2 domain) with FGR. Forms a multiprotein complex with LYN and ANKRD54. Expressed only in tissues and cells of hematopoietic origin. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Cellular Component: transcription factor complex; mitochondrion; cytoplasm; nucleus
Molecular Function: protein binding; protein complex binding; actin binding; SH3 domain binding; protein kinase binding
Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; regulation of actin filament polymerization; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of STAT protein; actin filament polymerization; positive regulation of cell proliferation; positive regulation of granulocyte differentiation; response to hormone stimulus; positive regulation of transcription factor import into nucleus; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of macrophage differentiation; positive regulation of peptidyl-serine phosphorylation
Reference #:  P49710 (UniProtKB)
Alt. Names/Synonyms: AW213261; Hcls1; hematopoietic cell specific Lyn substrate 1; Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; Hs1; LckBP1
Gene Symbols: Hcls1
Molecular weight: 54,240 Da
Basal Isoelectric point: 4.75  Predict pI for various phosphorylation states
CST Pathways:  B Cell Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

HS1

Protein Structure Not Found.


STRING  |  BioGPS  |  Scansite  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
intracellular localization: Y388‑p, Y405‑p
molecular association, regulation: Y388‑p, Y405‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
0 1 S13 VGHDVSVSVETQGDD
1 4 T16 DVSVSVETQGDDWDT
1 13 T23 TQGDDWDTDPDFVND
0 2 S32 PDFVNDISEKEQRWG
0 1 K34 FVNDISEKEQRWGAK
0 2 K41 KEQRWGAKTIEGSGR
0 11 T42 EQRWGAKTIEGSGRT
0 1 K60 NIHQLRNKVSEEHDI
0 1 S62 HQLRNKVSEEHDILK
0 6 Y83 GPKASHGYGGRFGVE
0 1 S97 ERDRMDKSAVGHEyV
0 134 Y103-p KSAVGHEyVADVEKH
0 1 K109 EyVADVEKHSSQTDA
0 1 R118 SSQTDAARGFGGKYG
0 2 K123 AARGFGGKYGVERDR
0 30 Y124 ARGFGGKYGVERDRA
0 3 S134 ERDRADKSAVGFDyK
0 97 Y140-p KSAVGFDyKGEVEKH
0 1 K141 SAVGFDyKGEVEKHA
0 1 K151 VEKHASQKDySHGFG
0 12 Y153-p KHASQKDySHGFGGR
0 8 S154 HASQKDySHGFGGRY
0 4 Y161 SHGFGGRYGVEKDKR
0 1 K165 GGRYGVEKDKRDKAA
0 150 Y175-p RDKAALGyDyKGETE
0 112 Y177-p KAALGyDyKGETEKH
0 1 K178 AALGyDyKGETEKHE
0 1 T181 GyDyKGETEKHESQR
0 1 K183 DyKGETEKHESQRdY
0 1 S186 GETEKHESQRdYAKG
0 1 D189-ca EKHESQRdYAKGFGG
0 47 Y190 KHESQRdYAKGFGGQ
0 2 K192 ESQRdYAKGFGGQyG
0 265 Y198-p AKGFGGQyGIQKDRV
0 1 K202 GGQyGIQKDRVDKSA
0 2 S208 QKDRVDKSAVGFNEM
0 1 T219 FNEMEAPTTAyKKTT
0 1 T220 NEMEAPTTAyKKTTP
4 144 Y222-p MEAPTTAyKKTTPIE
0 2 K223 EAPTTAyKKTTPIEA
0 1 K224 APTTAyKKTTPIEAA
0 1 K239 SSGARGLKAKFESLA
0 2 K241 GARGLKAKFESLAEE
0 1 K257 RKREEEEKAQQMARQ
0 1 V272 QQERKAVVKMSREVQ
0 1 K273 QERKAVVKMSREVQQ
0 35 S275 RKAVVKMSREVQQPS
0 7 S308 EVWPPAESHLPPESQ
0 5 Y323-p PVRSRREyPVPsLPt
0 1 S327-p RREyPVPsLPtRQsP
0 1 T330-p yPVPsLPtRQsPLQN
0 6 S333-p PsLPtRQsPLQNHLE
0 18 Y364-p QVVEEPVyEAAPELE
0 1 T384-p DYEPEPEtEPDyEDV
8 39 Y388-p EPEtEPDyEDVGELD
9 8 Y405-p DEDAEGDyEDVLEPE
0 4 Y482 FGLFPANYVKLL___
8714 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb
11880 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb (PE Conjugate)
13708 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
  human

 
S13-p VGHDVSVsVEtQGDD
T16-p DVSVsVEtQGDDWDt
T23-p tQGDDWDtDPDFVND
S32-p PDFVNDIsEkEQRWG
K34-ub FVNDIsEkEQRWGAk
K41-ac kEQRWGAktIEGSGR
T42-p EQRWGAktIEGSGRT
K60-ub NIHQLRNkVsEEHDV
S62-p HQLRNkVsEEHDVLR
Y83-p GPKASHGyGGRFGVE
S97-p ERDRMDKsAVGHEyV
Y103-p KsAVGHEyVAEVEKH
K109 EyVAEVEKHSSQTDA
K118 SSQTDAAKGFGGkyG
K123-ac AAKGFGGkyGVERDR
Y124-p AKGFGGkyGVERDRA
S134-p ERDRADKsAVGFDyk
Y140-p KsAVGFDykGEVEKH
K141-ac sAVGFDykGEVEKHT
K151 VEKHTSQKDysRGFG
Y153-p KHTSQKDysRGFGGR
S154-p HTSQKDysRGFGGRy
Y161-p sRGFGGRyGVEKDKW
K165 GGRyGVEKDKWDKAA
Y175-p WDKAALGyDyKGEtE
Y177-p KAALGyDyKGEtEKH
K178 AALGyDyKGEtEKHE
T181-p GyDyKGEtEKHEsQR
K183 DyKGEtEKHEsQRDy
S186-p GEtEKHEsQRDyAkG
D189 EKHEsQRDyAkGFGG
Y190-p KHEsQRDyAkGFGGQ
K192-ac EsQRDyAkGFGGQyG
Y198-p AkGFGGQyGIQKDRV
K202 GGQyGIQKDRVDKsA
S208-p QKDRVDKsAVGFNEM
T219-p FNEMEAPttAykkTT
T220-p NEMEAPttAykkTTP
Y222-p MEAPttAykkTTPIE
K223-ac EAPttAykkTTPIEA
K224-ac APttAykkTTPIEAA
K239-ub SSGTRGLkAkFESMA
K241-ac GTRGLkAkFESMAEE
K257 RKREEEEKAQQVARR
T272-p QQERKAVtKRsPEAP
K273 QERKAVtKRsPEAPQ
S275-p RKAVtKRsPEAPQPV
T308-p EAWPPVGtPPSSESE
H323 PVRTSREHPVPLLPI
L327 SREHPVPLLPIRQtL
I330 HPVPLLPIRQtLPED
T333-p PLLPIRQtLPEDNEE
Y360-p QVEEEPVyEAEPEPE
P374 EPEPEPEPENDyEDV
Y378-p EPEPENDyEDVEEMD
Y397-p EDEPEGDyEEVLEPE
Y481-p FGLFPANyVKLLE__
4507 : Phospho-HS1 (Tyr397) Antibody
8714 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb
11880 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb (PE Conjugate)
13090 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
13708 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
13742 : Phospho-HS1 (Tyr397) (D12C1) XP(R) Rabbit mAb (Pacific Blue(TM) Conjugate)
  rat

 
S13 VGHNVSVSVETQGDD
T16 NVSVSVETQGDDWDT
T23 TQGDDWDTDPDFVND
S32 PDFVNDISEKEQRWG
K34 FVNDISEKEQRWGAk
K41-ac KEQRWGAkTIEGSGR
T42 EQRWGAkTIEGSGRT
K60 NIHQLRNKVsEEHDI
S62-p HQLRNKVsEEHDILK
Y83 GPKASHGYGGRFGVE
S97 ERDRMDKSAVGHEYV
Y103 KSAVGHEYVAEVEkH
K109-ac EYVAEVEkHSSQTDA
K118-ac SSQTDAAkGFGGkYG
K123-ac AAkGFGGkYGVERDR
Y124 AkGFGGkYGVERDRA
S134 ERDRADKSAVGFDYK
Y140 KSAVGFDYKGEVEKH
K141 SAVGFDYKGEVEKHA
K151-ac VEKHASQkDYSHGFG
Y153 KHASQkDYSHGFGGR
S154 HASQkDYSHGFGGRY
Y161 SHGFGGRYGVEkDKR
K165-ac GGRYGVEkDKRDKAA
Y175 RDKAALGYDYkGETE
Y177 KAALGYDYkGETEkH
K178-ac AALGYDYkGETEkHE
T181 GYDYkGETEkHESQR
K183-ac DYkGETEkHESQRDY
S186 GETEkHESQRDYAkG
D189 EkHESQRDYAkGFGG
Y190 kHESQRDYAkGFGGQ
K192-ac ESQRDYAkGFGGQYG
Y198 AkGFGGQYGIQkDRV
K202-ac GGQYGIQkDRVDKSA
S208 QkDRVDKSAVGFNEM
T219 FNEMEAPTTAYKKTT
T220 NEMEAPTTAYKKTTP
Y222 MEAPTTAYKKTTPIE
K223 EAPTTAYKKTTPIEA
K224 APTTAYKKTTPIEAA
K239 SSGARGLKAkFESMA
K241-ac GARGLKAkFESMAEE
K257-ac RKREEEEkAQQMARQ
V272 QQERKAMVkMSREAQ
K273-ac QERKAMVkMSREAQQ
S275 RKAMVkMSREAQQPS
S308 EVWPPAESHLPPQSE
Y323 PVGGRREYPVPSLPT
S327 RREYPVPSLPTRQPP
T330 YPVPSLPTRQPPPQN
P333 PSLPTRQPPPQNPLE
Y364 QVVEEPVYEAAPEPE
- gap
Y378 EPEPEPDYEDVGELD
Y395 DEEAEGDYEDVLEPE
Y472 FGLFPANYVKLL___
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