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Protein Page:
ARRB1 (human)

Overview
ARRB1 regulates G-protein coupled receptors (GPCR) signaling by mediating both receptor desensitization and resensitization processes. Binds to GRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; the binding appears to require receptor determinants exposed only in the active receptor conformation. Targets many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins). Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins. Two different modes of arrestin-mediated internalization occur. Beta-arrestins function as multivalent adapter proteins that can switch the GPCR from a G-protein signaling mode that transmits short-lived signals from the plasma membrane via small molecule second messengers and ion channels to a beta-arrestin signaling mode that transmits a distinct set of signals that are initiated as the receptor internalizes and transits the intracellular compartment. Also involved in regulation of receptors other than GPCRs. Involved in Toll-like receptor and IL-1 receptor signaling through the interaction with TRAF6 which prevents TRAF6 autoubiquitination and oligomerization required for activation of NF-kappa-B and JUN. Binds phosphoinositides. Binds inositolhexakisphosphate (InsP6). Involved in IL8-mediated granule release in neutrophils. Interacts with phosphorylated ADRB2 and CHRM2. Interacts with SRC (via the SH3 domain and the protein kinase domain); the interaction is independent of the phosphorylation state of SRC C-terminus. Interacts with RAF1, CHUK, IKBKB and Nik. Interacts with DVL1 and DVL2; the interaction is enhanced by DVL phosphorylation. Interacts with IGF1R. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB..
Protein type: Adaptor/scaffold
Cellular Component: Golgi membrane; cytoplasmic vesicle membrane; lysosomal membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; coated pit; cytoplasmic vesicle; pseudopodium; cytosol; nucleus; chromatin
Molecular Function: angiotensin receptor binding; enzyme inhibitor activity; insulin-like growth factor receptor binding; protein binding; histone acetyltransferase activity; mitogen-activated protein kinase kinase binding; ubiquitin protein ligase binding; caspase inhibitor activity; transcription factor binding; GTPase activator activity
Biological Process: transcription from RNA polymerase II promoter; platelet activation; proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; positive regulation of protein binding; positive regulation of receptor internalization; positive regulation of Rho protein signal transduction; protein ubiquitination; positive regulation of peptidyl-serine phosphorylation; protein transport; G-protein coupled receptor internalization; inhibition of NF-kappaB transcription factor; negative regulation of interleukin-6 production; stress fiber formation; phototransduction; negative regulation of interleukin-8 production; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; positive regulation of protein amino acid phosphorylation; post-Golgi vesicle-mediated transport; blood coagulation; positive regulation of GTPase activity
Reference #:  P49407 (UniProtKB)
Alt. Names/Synonyms: ARB1; ARR1; ARRB1; arrestin 2; Arrestin beta-1; arrestin, beta 1; beta-arrestin; Beta-arrestin-1
Gene Symbols: ARRB1
Molecular weight: 47,066 Da
Basal Isoelectric point: 5.84  Predict pI for various phosphorylation states
CST Pathways:  GPCR Signaling to MAPKs  |  Hedgehog Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ARRB1

Protein Structure Not Found.


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Sites Implicated In
activity, induced: S412‑p
activity, inhibited: S412‑p
molecular association, regulation: S412‑p

Modification Sites and Domains  

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 73 Y47-p VVLVDPEyLKERRVy
1 2 Y54-p yLKERRVyVTLTCAF
0 1 S86-p LFVANVQsFPPAPED
1 0 S163-p EKIHKRNsVRLVIRK
0 18 Y173-p LVIRKVQyAPERPGP
0 1 T410-p KEEEEDGtGsPQLNN
7 15 S412-p EEEDGtGsPQLNNR_
2416 : Phospho-beta-Arrestin 1 (Ser412) (6-24) Mouse mAb
  ARRB1 iso2  
Y47 VVLVDPEYLKERRVY
Y54 YLKERRVYVTLTCAF
S86 LFVANVQSFPPAPED
S163 EKIHKRNSVRLVIRK
Y173 LVIRKVQYAPERPGP
T402 KEEEEDGTGSPQLNN
S404 EEEDGTGSPQLNNR_
  mouse

 
Y47-p VVLVDPEyLKERRVY
Y54 yLKERRVYVTLTCAF
S86 LFVANVQSFPPAPED
S163 EKIHKRNSVRLVIRK
Y173 LVIRKVQYAPERPGP
T410-p KDEEDDGtGsPHLNN
S412-p EEDDGtGsPHLNNR_
  rat

 
Y47-p VVLVDPEyLKERRVy
Y54-p yLKERRVyVTLTCAF
S86 LFVANVQSFPPAPED
S163 EKIHKRNSVRLVIRK
Y173-p LVIRKVQyAPERPGP
T410-p KDEEDDGtGsPHLNN
S412-p EEDDGtGsPHLNNR_
2416 : Phospho-beta-Arrestin 1 (Ser412) (6-24) Mouse mAb
  cow

 
Y47 VVLVDPEYLKERRVY
Y54 YLKERRVYVTLTCAF
S86 LFVANVQSFPPAPED
S163 EKIHKRNSVRLVIRK
Y173 LVIRKVQYAPERPGP
T410 KEEEEDGTGSPRLND
S412 EEEDGTGSPRLNDR_
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