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Protein Page:
ARRB1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ARRB1 regulates G-protein coupled receptors (GPCR) signaling by mediating both receptor desensitization and resensitization processes. Binds to GRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; the binding appears to require receptor determinants exposed only in the active receptor conformation. Targets many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins). Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins. Two different modes of arrestin-mediated internalization occur. Beta-arrestins function as multivalent adapter proteins that can switch the GPCR from a G-protein signaling mode that transmits short-lived signals from the plasma membrane via small molecule second messengers and ion channels to a beta-arrestin signaling mode that transmits a distinct set of signals that are initiated as the receptor internalizes and transits the intracellular compartment. Also involved in regulation of receptors other than GPCRs. Involved in Toll-like receptor and IL-1 receptor signaling through the interaction with TRAF6 which prevents TRAF6 autoubiquitination and oligomerization required for activation of NF-kappa-B and JUN. Binds phosphoinositides. Binds inositolhexakisphosphate (InsP6). Involved in IL8-mediated granule release in neutrophils. Interacts with phosphorylated ADRB2 and CHRM2. Interacts with SRC (via the SH3 domain and the protein kinase domain); the interaction is independent of the phosphorylation state of SRC C-terminus. Interacts with RAF1, CHUK, IKBKB and Nik. Interacts with DVL1 and DVL2; the interaction is enhanced by DVL phosphorylation. Interacts with IGF1R. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB..
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: Golgi membrane; nucleoplasm; cytoplasmic vesicle membrane; lysosomal membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; coated pit; pseudopodium; cytoplasmic vesicle; nucleus; chromatin; cytosol
Molecular Function: angiotensin receptor binding; enzyme inhibitor activity; insulin-like growth factor receptor binding; protein binding; histone acetyltransferase activity; mitogen-activated protein kinase kinase binding; ubiquitin protein ligase binding; caspase inhibitor activity; transcription factor binding; GTPase activator activity
Biological Process: transcription from RNA polymerase II promoter; platelet activation; proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; positive regulation of protein binding; positive regulation of receptor internalization; positive regulation of Rho protein signal transduction; protein ubiquitination; negative regulation of caspase activity; positive regulation of peptidyl-serine phosphorylation; protein transport; G-protein coupled receptor internalization; inhibition of NF-kappaB transcription factor; stress fiber formation; negative regulation of interleukin-8 production; negative regulation of interleukin-6 production; phototransduction; positive regulation of histone acetylation; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; post-Golgi vesicle-mediated transport; blood coagulation; positive regulation of GTPase activity
Reference #:  P49407 (UniProtKB)
Alt. Names/Synonyms: ARB1; ARR1; ARRB1; arrestin 2; Arrestin beta-1; arrestin, beta 1; beta-arrestin; Beta-arrestin-1
Gene Symbols: ARRB1
Molecular weight: 47,066 Da
Basal Isoelectric point: 5.84  Predict pI for various phosphorylation states
CST Pathways:  GPCR Signaling to MAPKs  |  Hedgehog Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ARRB1

Protein Structure Not Found.


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Sites Implicated In
activity, induced: S412‑p
activity, inhibited: S412‑p
molecular association, regulation: S412‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 K17 KKASPNGKLTVYLGK
0 73 Y47-p VVLVDPEyLKERRVy
1 2 Y54-p yLKERRVyVTLTCAF
0 1 S86-p LFVANVQsFPPAPED
0 1 Y144-p GKACGVDyEVKAFCA
1 0 S163-p EKIHKRNsVRLVIRK
0 18 Y173-p LVIRKVQyAPERPGP
0 1 T350 VAVELPFTLMHPKPK
0 3 T410-p KEEEEDGtGsPQLNN
7 24 S412-p EEEDGtGsPQLNNR_
2416 : Phospho-beta-Arrestin 1 (Ser412) (6-24) Mouse mAb
  ARRB1 iso2  
K17 KKASPNGKLTVYLGK
Y47 VVLVDPEYLKERRVY
Y54 YLKERRVYVTLTCAF
S86 LFVANVQSFPPAPED
Y144 GKACGVDYEVKAFCA
S163 EKIHKRNSVRLVIRK
Y173 LVIRKVQYAPERPGP
T342-p VAVELPFtLMHPKPK
T402 KEEEEDGTGSPQLNN
S404 EEEDGTGSPQLNNR_
  mouse

 
K17 KKASPNGKLTVYLGK
Y47-p VVLVDPEyLKERRVY
Y54 yLKERRVYVTLTCAF
S86 LFVANVQSFPPAPED
Y144 GKACGVDYEVKAFCA
S163 EKIHKRNSVRLVIRK
Y173 LVIRKVQYAPERPGP
T350 VAVELPFTLMHPKPK
T410-p KDEEDDGtGsPHLNN
S412-p EEDDGtGsPHLNNR_
  rat

 
K17-ac KKASPNGkLTVYLGK
Y47-p VVLVDPEyLKERRVy
Y54-p yLKERRVyVTLTCAF
S86 LFVANVQSFPPAPED
Y144 GKACGVDYEVKAFCA
S163 EKIHKRNSVRLVIRK
Y173-p LVIRKVQyAPERPGP
T350 VAVELPFTLMHPKPK
T410-p KDEEDDGtGsPHLNN
S412-p EEDDGtGsPHLNNR_
2416 : Phospho-beta-Arrestin 1 (Ser412) (6-24) Mouse mAb
  cow

 
K17 KKASPNGKLTVYLGK
Y47 VVLVDPEYLKERRVY
Y54 YLKERRVYVTLTCAF
S86 LFVANVQSFPPAPED
Y144 GKACGVDYEVKAFCA
S163 EKIHKRNSVRLVIRK
Y173 LVIRKVQYAPERPGP
T350 VAVELPFTLMHPKPK
T410 KEEEEDGTGSPRLND
S412 EEEDGTGSPRLNDR_
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