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Protein Page:
Mena (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
Mena Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes. Homotetramer. Interacts with APBB1IP, APBB1, PFN1 and ROBO4. Isoforms, containing the polyproline-rich regions with PPLP motifs, bind the WW domain of APBB1IP. Isoforms, containing the PPSY motif, bind, in vitro, to the WW2 and WW3 domains of NEDD4 and to the WW1 domain of YAP1. Binds the SH3 domain of BAIAP2-alpha but only after the autoinhibitory region of BAIAP2-alpha has been blocked by interaction with CDC42. Interacts, via the EVH1/WH1 domain, with the Pro-rich domains from VCL, ZYX and Listeria monocytogenes actA and with TES (via LIM domains). The TES LIM domain and the Pro-rich domains from VCL or ZYX compete for the same binding site. Interaction with ZYX is important for targeting ENAH to focal adhesions and enhances production of actin-rich structures at the apical surface of cells. Interacts, through the Pro-rich region, with the C-terminal SH3 domain of DNMPB. Binds GPHN. Interacts with FAT1 (via EVH1 domains). Heterotrimer with TES and ACTL7A. Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions. Belongs to the Ena/VASP family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold
Cellular Component: focal adhesion; lamellipodium; cytoplasm; plasma membrane; stress fiber; synapse; cytosol; cell junction; filopodium
Molecular Function: WW domain binding; actin binding; SH3 domain binding
Biological Process: axon guidance; intracellular transport; actin polymerization and/or depolymerization; neural tube closure; T cell receptor signaling pathway
Reference #:  Q8N8S7 (UniProtKB)
Alt. Names/Synonyms: ENA; enabled homolog (Drosophila); ENAH; MENA; NDPP1; Protein enabled homolog
Gene Symbols: ENAH
Molecular weight: 66,510 Da
Basal Isoelectric point: 6.51  Predict pI for various phosphorylation states
CST Pathways:  Regulation of Actin Dynamics
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Mena
Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

► Hide Isoforms 		 
0 1 Y16-p ARAAVMVyDDANKKW
0 2 K69-u CAIPKGLkYNQATQT
0 1 T116-p EVLNSQEtGPtLPRQ
0 1 T119-p NSQEtGPtLPRQNsQ
0 72 S125-p PtLPRQNsQLPAQVQ
3 18 S265-p WERERRIssAAAPAS
0 1 - gap
0 1 - gap
0 1 - gap
0 5 - gap
0 1 - gap
0 1 - gap
0 3 - gap
0 1 - gap
0 8 S266-p ERERRIssAAAPASV
1 0 Q325 PLPPGPAQASVALPP
0 2 S405-p GAKLRKVsRMEDtSF
0 1 T410-p KVsRMEDtSFPSGGN
0 6 R432 SSKTDTGRGNGPLPL
0 4 T464-p RIAEKGStIETEQKE
0 2 T481-p GEDSEPVtSKAsstS
0 1 S482 EDSEPVtSKAsstSt
0 1 S485-p EPVtSKAsstStPEP
0 1 S486-p PVtSKAsstStPEPT
0 2 T487-p VtSKAsstStPEPTR
0 1 S488 tSKAsstStPEPTRK
0 29 T489-p SKAsstStPEPTRKP
0 8 T500-p TRKPWERtNtMNGsK
0 42 T502-p KPWERtNtMNGsKsP
0 17 S506-p RtNtMNGsKsPVIsR
0 58 S508-p NtMNGsKsPVIsRRD
0 7 S512-p GsKsPVIsRRDsPRK
0 2 S516-p PVIsRRDsPRKNQIV
0 1 S528-p QIVFDNRsyDSLHRP
0 1 Y529-p IVFDNRsyDSLHRPK
0 1 K574-u RKELTKLkEELIDAI
  Mena iso2  
Y16 ARAAVMVYDDANKKW
K69 CAIPKGLKYNQATQT
T116 EVLNSQETGPTLPRQ
T119 NSQETGPTLPRQNSQ
S125 PTLPRQNSQLPAQVQ
S265-p WERERRIssAAAPAS
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S266-p ERERRIssAAAPASV
Q325 PLPPGPAQASVALPP
S405 GAKLRKVSRMEDTSF
T410 KVSRMEDTSFPSGGN
R432 SSKTDTGRGNGPLPL
T464 RIAEKGSTIETEQKE
T481 GEDSEPVTSKASSTS
S482 EDSEPVTSKASSTSt
S485 EPVTSKASSTStPEP
S486 PVTSKASSTStPEPT
T487 VTSKASSTStPEPTR
S488 TSKASSTStPEPTRK
T489-p SKASSTStPEPTRKP
T500-p TRKPWERtNtMNGsK
T502-p KPWERtNtMNGsKsP
S506-p RtNtMNGsKsPVIsR
S508-p NtMNGsKsPVIsRPK
S512-p GsKsPVIsRPKSTPL
- gap
- gap
- gap
K553 RKELTKLKEELIDAI
  Mena iso5  
Y16 ARAAVMVYDDANKKW
K69 CAIPKGLKYNQATQT
T116 EVLNSQETAQSKVTA
T138 RCIFCGPTLPRQNsQ
S144-p PTLPRQNsQLPAQVQ
S284-p WERERRIsSAAPSSD
T356 HSATRFATSLGSAFH
S357 SATRFATSLGSAFHP
S380-p PRPLNKNsRPSsPVN
S384-p NKNsRPSsPVNtPSS
T388-p RPSsPVNtPSSQPPA
T396-p PSSQPPAtKPCAWST
S412 NFSPLPPSPPIMISS
S419 SPPIMISSPPGKATG
S513 PSVLPSPSAAAPASV
Q572 PLPPGPAQASVALPP
S652 GAKLRKVSRMEDTSF
T657 KVSRMEDTSFPSGGN
R679 SSKTDTGRGNGPLPL
T711 RIAEKGSTIETEQKE
T728 GEDSEPVTSKASSTS
S729 EDSEPVTSKASSTSt
S732 EPVTSKASSTStPEP
S733 PVTSKASSTStPEPT
T734 VTSKASSTStPEPTR
S735 TSKASSTStPEPTRK
T736-p SKASSTStPEPTRKP
T747-p TRKPWERtNtMNGsK
T749-p KPWERtNtMNGsKsP
S753-p RtNtMNGsKsPVIsR
S755-p NtMNGsKsPVIsRPK
S759-p GsKsPVIsRPKSTPL
- gap
- gap
- gap
K800 RKELTKLKEELIDAI
  mouse

► Hide Isoforms 		 
Y16 ARAAVMVYDDANKKW
K69-u CAIPKGLkYNQATQT
A116 EVLNSQEAAQSKVTA
T138 RCIFCGPTLPRQNsQ
S144-p PTLPRQNsQLPAQVQ
S255-p WERERRMsNAAPSSD
T327-p HAATRFAtsLGSAFH
S328-p AATRFAtsLGSAFHP
S351 PRPLNKNSRPSsPVN
S355-p NKNSRPSsPVNTPSS
T359 RPSsPVNTPSSQPPA
A367 PSSQPPAAKSCAWPT
S383-p NFSPLPPsPPIMISs
S390-p sPPIMISsPPGKATG
S498 PSVLPSPSAGAPASA
Y557 PLPSGPAYASALPPP
S637-p GAKLRKVsRVEDGSF
G642 KVsRVEDGSFPGGGN
R664-m1 SSKADAGrGNGPLPL
T696-p RIAEKGStIETEQKE
T713 NEDAEPITAKAPSts
A714 EDAEPITAKAPStst
P717 EPITAKAPStstPEP
S718 PITAKAPStstPEPT
T719-p ITAKAPStstPEPTR
S720-p TAKAPStstPEPTRK
T721-p AKAPStstPEPTRKP
T732 TRKPWERTNtMNGsK
T734-p KPWERTNtMNGsKsP
S738-p RTNtMNGsKsPVIsR
S740-p NtMNGsKsPVIsRPK
S744-p GsKsPVIsRPKSTPS
- gap
- gap
- gap
K785 RKELAKLKEELIDAI
  Mena iso3  
Y16 ARAAVMVYDDANKKW
K69 CAIPKGLKYNQATQT
A116 EVLNSQEAGPTLPRQ
T119 NSQEAGPTLPRQNsQ
S125-p PTLPRQNsQLPAQVQ
S236 WERERRMSNAAAPAS
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
Y296-p PLPSGPAyASALPPP
S376 GAKLRKVSRVEDGSF
G381 KVSRVEDGSFPGGGN
R403 SSKADAGRGNGPLPL
T435 RIAEKGSTIETEQKE
T452 NEDAEPITAKAPSTS
A453 EDAEPITAKAPSTSt
P456 EPITAKAPSTStPEP
S457 PITAKAPSTStPEPT
T458 ITAKAPSTStPEPTR
S459 TAKAPSTStPEPTRK
T460-p AKAPSTStPEPTRKP
T471 TRKPWERTNtMNGsK
T473-p KPWERTNtMNGsKsP
S477-p RTNtMNGsKsPVISR
S479-p NtMNGsKsPVISRPK
S483 GsKsPVISRPKSTPS
- gap
- gap
- gap
K524 RKELAKLKEELIDAI
  Mena iso4  
Y16 ARAAVMVYDDANKKW
K69 CAIPKGLKYNQATQT
A116 EVLNSQEAGPTLPRQ
T119 NSQEAGPTLPRQNsQ
S125-p PTLPRQNsQLPAQVQ
S236-p WERERRMsNAAPSSD
T308 HAATRFATSLGSAFH
S309 AATRFATSLGSAFHP
S332 PRPLNKNSRPSSPVN
S336 NKNSRPSSPVNTPSS
T340 RPSSPVNTPSSQPPA
A348 PSSQPPAAKSCAWPT
S364-p NFSPLPPsPPIMISs
S371-p sPPIMISsPPGKATG
S479 PSVLPSPSAGAPASA
Y538 PLPSGPAYASALPPP
S618 GAKLRKVSRVEDGSF
G623 KVSRVEDGSFPGGGN
R645 SSKADAGRGNGPLPL
T677 RIAEKGSTIETEQKE
T694 NEDAEPITAKAPSTS
A695 EDAEPITAKAPSTSt
P698 EPITAKAPSTStPEP
S699 PITAKAPSTStPEPT
T700 ITAKAPSTStPEPTR
S701 TAKAPSTStPEPTRK
T702-p AKAPSTStPEPTRKP
T713 TRKPWERTNtMNGsK
T715-p KPWERTNtMNGsKsP
S719-p RTNtMNGsKsPVISR
S721-p NtMNGsKsPVISRPK
S725 GsKsPVISRPKSTPS
- gap
- gap
- gap
K766 RKELAKLKEELIDAI
  rat

 
Y36 ARAAVMVYDDANKKW
K89 CAIPKGLKYNQATQT
A136 EVLNSQEAGPTLPRQ
T139 NSQEAGPTLPRQNSQ
S145 PTLPRQNSQLPAQVQ
S258 WERERRMSNAGHVLG
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
Y281 PLPSGPAYASALPPP
S361 GAKLRKVSRVEDGSF
G366 KVSRVEDGSFPSGGN
R388 SSKTDSGRGNGPLPL
T420 RIAEKGSTIETEQKE
T437-p SEDAEPVtsKAASTS
S438-p EDAEPVtsKAASTST
A441 EPVtsKAASTSTPEP
S442 PVtsKAASTSTPEPT
T443 VtsKAASTSTPEPTR
S444 tsKAASTSTPEPTRK
T445 sKAASTSTPEPTRKP
T456 TRKPWERTNTMNGSK
T458 KPWERTNTMNGSKSP
S462 RTNTMNGSKSPVISR
S464 NTMNGSKSPVISRPK
S468 GSKSPVISRPKSTPS
- gap
- gap
- gap
K509 RKELAKLKEELIDAI
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