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Protein Page:
Ube2k (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Ube2k Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein- dependent degradation of RB1. Interacts with RNF138/NARF. Interacts with BRCA1. By aggregated low-density lipoprotein. Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ligase
Chromosomal Location of Human Ortholog: 4p14
Cellular Component: cytoplasm
Molecular Function: protein binding; acid-amino acid ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding
Biological Process: ubiquitin-dependent protein catabolic process; regulation of proteasomal ubiquitin-dependent protein catabolic process
Reference #:  P61086 (UniProtKB)
Alt. Names/Synonyms: DKFZp564C1216; DKFZp686J24237; E2(25K); E2-25K; HIP-2; HIP2; huntingtin interacting protein 2; Huntingtin-interacting protein 2; HYPG; LIG; UBE2K; Ubiquitin carrier protein; Ubiquitin-conjugating enzyme E2 K; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-conjugating enzyme E2-25K; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); Ubiquitin-protein ligase
Gene Symbols: UBE2K
Molecular weight: 22,407 Da
Basal Isoelectric point: 5.33  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Ube2k

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 K14-ac QRIKREFkEVLkSEE
1 0 K14-sm QRIKREFkEVLkSEE
0 1 K14-sc QRIKREFkEVLkSEE
0 1 K18 REFkEVLKSEETSkN
0 1 K18-ub REFkEVLkSEETSkN
0 3 K24-ub LkSEETSkNQIKVDL
0 2 K61-ub GRYQLEIkIPETYPF
0 6 K72-ub TYPFNPPkVRFITKI
0 8 K97-ub AICLDILkDQWAAAM
0 2 K142-ub KQNPEMFkQTARLWA
0 1 S159-p YAGAPVSsPEYTkkI
0 24 K164-ub VSsPEYTkkIENLCA
0 59 K165-ub SsPEYTkkIENLCAM
0 1 S185-p AVIVALSsKSWDVET
  mouse

 
K14 QRIKREFKEVLkSEE
K14 QRIKREFKEVLkSEE
K14 QRIKREFKEVLkSEE
K18-ac REFKEVLkSEETSkN
K18 REFKEVLKSEETSkN
K24-ub LkSEETSkNQIKVDL
K61-ub GRYQLEIkIPETYPF
K72-ub TYPFNPPkVRFITKI
K97-ub AICLDILkDQWAAAM
K142 KQNPEMFKQTARLWA
S159 YAGAPVSSPEYTkkI
K164-ub VSSPEYTkkIENLCA
K165-ub SSPEYTkkIENLCAM
S185 AVIVALSSKSWDVET
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