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Protein Page:
CAD (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
CAD a multifunctional enzyme that catalyzes four enzymatic activities of the pyrimidine pathway, including the rate-limiting first step. The four enzymatic activities involved in de novo pyrimidine synthesis are glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase activities. Provides essential precursors for DNA synthesis. Required for the de novo synthesis of pyrimidine nucleotides and cell growth. Phosphorylation by p70S6K promotes CAD oligomerization, stimulating de novo synthesis of pyrimidines and progression through S phase in mammalian cells. Utilizes glutamine, bicarbonate, and aspartic acid to form a pyrimidine ring that is subsequently attached to ribose to generate a pyrimidine. Note: This description may include information from UniProtKB.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; Ligase; EC 6.3.5.5; EC 2.1.3.2; EC 3.5.2.3; Nucleotide Metabolism - pyrimidine; Hydrolase
Chromosomal Location of Human Ortholog: 2p22-p21
Cellular Component: cell projection; protein complex; nuclear matrix; membrane; cell soma; terminal button; nucleus; cytosol
Molecular Function: identical protein binding; enzyme binding; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; zinc ion binding; aspartate carbamoyltransferase activity; ATP binding; dihydroorotase activity; protein kinase activity
Biological Process: lactation; organ regeneration; nucleobase, nucleoside and nucleotide metabolic process; protein amino acid autophosphorylation; UTP biosynthetic process; peptidyl-threonine phosphorylation; glutamine metabolic process; response to testosterone stimulus; female pregnancy; response to caffeine; 'de novo' pyrimidine base biosynthetic process; embryonic development; response to cortisol stimulus; pyrimidine base metabolic process; glutamine catabolic process; pyrimidine nucleoside biosynthetic process; drug metabolic process; response to amine stimulus
Reference #:  P27708 (UniProtKB)
Alt. Names/Synonyms: Aspartate carbamoyltransferase; CAD; CAD protein; CAD trifunctional protein; carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase; Dihydroorotase; Glutamine-dependent carbamoyl-phosphate synthase; multifunctional protein CAD; PYR1
Gene Symbols: CAD
Molecular weight: 242,984 Da
Basal Isoelectric point: 6.02  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

CAD

Protein Structure Not Found.


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Sites Implicated In
enzymatic activity, induced: S1859‑p
intracellular localization: T456‑p
phosphorylation: S1406‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S10-p ALVLEDGsVLRGQPF
0 1 K137-ub EQGSLLGkLVQNGTE
0 1 K165-ub LVPEVSIktPRVFNt
0 2 T166-p VPEVSIktPRVFNtG
0 1 T172-p ktPRVFNtGGAPRIL
0 2 K186-ac LALDCGLkYNQIRCL
0 1 K186-ub LALDCGLkYNQIRCL
0 1 Y267-p LAIGAKTyKMRYGNR
0 1 K424-ub SQAIKALkEENIQTL
6 0 T456-p KVYFLPItPHYVTQV
0 1 T475-p RPDGVLLtFGGQTAL
0 1 T504-p YGVRVLGtPVETIEL
0 1 T585-p VAPAFAHtsQVLVDk
0 1 S586-p APAFAHtsQVLVDkS
0 1 K592-ub tsQVLVDkSLKGWKE
0 3 K696-ub RSSALASkATGYPLA
0 6 Y735-p AFEPSVDyCVVkIPR
0 5 K739-ub SVDyCVVkIPRWDLS
0 1 K747-ac IPRWDLSkFLRVSTk
0 5 K747-ub IPRWDLSkFLRVSTk
0 2 K754-ub kFLRVSTkIGSCMKs
0 1 K760 TkIGSCMKsVGEVMG
0 1 S761-p kIGSCMKsVGEVMGI
0 4 K778-ub SFEEAFQkALRMVDE
0 1 K867 PDLLQQAKCLGFSDK
0 2 T883-p IALAVLStELAVRKL
0 1 T978 MVNYNPETVSTDYDM
0 1 S980 NYNPETVSTDYDMCD
0 1 T981 YNPETVSTDYDMCDR
0 1 Y983 PETVSTDYDMCDRLY
1 0 T1037 QQCRVLGTsPEAIDs
0 6 S1038-p QCRVLGTsPEAIDsA
0 1 S1044-p TsPEAIDsAENRFKF
0 1 S1061-p LLDTIGIsQPQWREL
0 4 K1119-ub SSAAAVSkEHPVVIS
0 2 K1127-ub EHPVVISkFIQEAKE
0 2 K1211-ub IAKDDQLkVIECNVR
0 1 S1220-p IECNVRVsRsFPFVS
0 1 S1222-p CNVRVsRsFPFVSkT
0 6 K1228-ub RsFPFVSkTLGVDLV
0 1 T1239 VDLVALATRVIMGEE
0 2 K1262-ub GSGVVGVkVPQFsFS
0 2 S1267-p GVkVPQFsFSRLAGA
0 5 K1309-ub AMLSTGFkIPKkNIL
0 3 K1313-ub TGFkIPKkNILLTIG
0 4 K1323-ub LLTIGSYkNkSELLP
0 11 K1325-ub TIGSYkNkSELLPTV
0 2 K1387-ub ILEQLAEkNFELVIN
3 20 S1406-p GAGGRRLssFVTkGY
0 4 S1407-p AGGRRLssFVTkGYR
0 2 K1411-ac RLssFVTkGYRTRRL
0 31 K1411-ub RLssFVTkGYRTRRL
0 1 S1423-p RRLAADFsVPLIIDI
0 12 K1431-ub VPLIIDIkCTkLFVE
0 6 K1434-ub IIDIkCTkLFVEALG
0 6 K1450-ub IGPAPPLkVHVDCMT
0 3 K1460-ub VDCMTSQkLVRLPGL
0 3 K1521-ub PALALAQkLAEAGAR
0 1 K1618-ub HICHVARkEEILLIk
0 1 K1625-ub kEEILLIkAAKARGL
0 7 K1657-ub LERLGPGkGEVRPEL
0 2 S1724-p AVSEGRLsLDDLLQR
0 1 Y1794-p VLRGEVAyIDGQVLV
0 1 Y1805-p QVLVPPGyGQDVRKW
0 3 S1823-p AVPQLPPsAPAtsEM
0 3 T1827-p LPPsAPAtsEMtttP
0 10 S1828-p PPsAPAtsEMtttPE
0 10 T1831-p APAtsEMtttPERPR
0 5 T1832-p PAtsEMtttPERPRR
0 13 T1833-p AtsEMtttPERPRRG
1 0 H1856-p FHLPPRIhRAsDPGL
3 130 S1859-p PPRIhRAsDPGLPAE
0 21 T1884-p AEPELMGtPDGtCyP
0 1 T1888-p LMGtPDGtCyPPPPV
0 11 Y1890-p GtPDGtCyPPPPVPR
0 13 S1900-p PPVPRQAsPQNLGtP
0 2 T1906-p AsPQNLGtPGLLHPQ
0 1 T1914-p PGLLHPQtSPLLHSL
0 4 S1938-p QFTKDQMsHLFNVAH
0 1 K1961-ub ERSLDILkGKVMASM
0 1 S2089 KHGRTVHSLACLLTQ
0 3 Y2103-p QYRVSLRyVAPPSLR
0 1 S2108 LRyVAPPSLRMPPTV
0 1 T2114 PSLRMPPTVRAFVAS
0 1 K2125-ub FVASRGTkQEEFESI
0 1 S2131 TkQEEFESIEEALPD
0 2 S2154-p IQKERFGstQEyEAC
0 3 T2155-p QKERFGstQEyEACF
0 43 Y2158-p RFGstQEyEACFGQF
0 1 Y2203-p DSDPRAAyFRQAENG
0 2 Y2212-p RQAENGMyIRMALLA
0 1 T2220-p IRMALLAtVLGRF__
12662 : Phospho-CAD (Ser1859) Antibody
  mouse

► Hide Isoforms
 
S10 ALVLEDGSVLQGRPF
K137 EQGSLLGKLVQKGTE
K165 LAPEVSIKTPRVFNA
T166 APEVSIKTPRVFNAG
A172 KTPRVFNAGGAPRIC
K186 CALDCGLKYNQIRCL
K186 CALDCGLKYNQIRCL
Y267 LAIGAKTYKMRYGNR
K424 SQAIKALKEENIQTL
T456 KVYFLPITLHYVTQV
T475 RPDGVLLTFGGQTAL
T504 YGVRVLGTPVETIEL
T585 VAPAFAHTSQVLIDK
S586 APAFAHTSQVLIDKS
K592 TSQVLIDKSLKGWKE
K696-ub RSSALASkATGYPLA
Y735 AFEPSLDYCVVKIPR
K739 SLDYCVVKIPRWDLS
K747 IPRWDLSKFLRVSTK
K747 IPRWDLSKFLRVSTK
K754 KFLRVSTKIGSCMkS
K760-ub TKIGSCMkSVGEVMG
S761 KIGSCMkSVGEVMGI
K778-ub SFEEAFQkALRMVDE
K867-ub QDLLHQAkCLGFSDK
T883 IALAVLSTELAVRKL
T978-p MVNYNPEtVstDyDM
S980-p NYNPEtVstDyDMCD
T981-p YNPEtVstDyDMCDR
Y983-p PEtVstDyDMCDRLY
T1037 QQCRVLGTsPEAIDS
S1038-p QCRVLGTsPEAIDSA
S1044 TsPEAIDSAENRFKF
S1061 LLDTIGISQPQWREL
K1119 SSAAAVSKEHPVVIS
K1127 EHPVVISKFIQEAKE
K1211 IAKDDQLKVIECNVR
S1220 IECNVRVSRSFPFVS
S1222 CNVRVSRSFPFVSkT
K1228-ub RSFPFVSkTLGVDLV
T1239-p VDLVALAtRIIMGEK
K1262 GSGVVGVKVPQFSFS
S1267 GVKVPQFSFSRLAGA
K1309 AMLSTGFKIPEKNIL
K1313 TGFKIPEKNILLTIG
K1323 LLTIGSYKNKSELLP
K1325 TIGSYKNKSELLPTV
N1387 ILDQLAENHFELVIN
S1406-p GAGGRRLssFVTKGY
S1407-p AGGRRLssFVTKGYR
K1411 RLssFVTKGYRTRRL
K1411 RLssFVTKGYRTRRL
S1423 RRLAADFSVPLIIDI
K1431 VPLIIDIKCTKLFVE
K1434 IIDIKCTKLFVEALG
K1450 IGPAPPLKVHVDCMT
K1460 VDCMTSQKLVRLPGL
K1521 PALALAQKLAEAGAR
K1618 HICHVARKEEILLIK
K1625 KEEILLIKTAKAQGL
K1657 LERLGPGKGEVRPEL
S1724-p AVSEGRLsLDDLLQR
Y1794 VLRGEVAYIDGQVLV
Y1805 QVLVPPGYGQDVRKW
S1823-p VVPQPPPsTPAttEI
T1827-p PPPsTPAttEItttP
T1828-p PPsTPAttEItttPE
T1831-p TPAttEItttPERPR
T1832-p PAttEItttPERPRR
T1833-p AttEItttPERPRRV
H1856 FHLPPRIHRAsDPGL
S1859-p PPRIHRAsDPGLPAE
T1884-p VEPELMGtPDGPCYP
P1888 LMGtPDGPCYPAPPV
Y1890 GtPDGPCYPAPPVPR
S1900-p PPVPRQAsPQNLGSS
S1906 AsPQNLGSSGLLHPQ
M1914 SGLLHPQMSPLLHSL
S1938-p QFTKDQMsHLFNVAH
K1961 ERSLDILKGKVMASM
S2089-p KHGRTVHsLACLLTQ
Y2103-p QYRVSLRyVAPPsLR
S2108-p LRyVAPPsLRMPPsV
S2114-p PsLRMPPsVRDFVAS
K2125 FVASRGTKQEEFEsI
S2131-gl TKQEEFEsIEEALPD
S2154 IQKERFGSVQEYEAC
V2155 QKERFGSVQEYEACF
Y2158 RFGSVQEYEACFGQF
Y2203 DSDPRAAYFRQAENG
Y2212 RQAENGMYIRMALLA
T2220 IRMALLATVLGRF__
12662 : Phospho-CAD (Ser1859) Antibody
  CAD iso2  
- gap
- gap
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- gap
- gap
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- gap
- gap
- gap
- gap
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- gap
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- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
Y67 VLRGEVAYIDGQVLV
Y78 QVLVPPGYGQDVRKW
S96 VVPQPPPSTPATTEI
T100 PPPSTPATTEITTTP
T101 PPSTPATTEITTTPE
T104 TPATTEITTTPERPR
T105 PATTEITTTPERPRR
T106 ATTEITTTPERPRRV
H129 FHLPPRIHRAsDPGL
S132-p PPRIHRAsDPGLPAL
T174 VEPELMGTPDGPCYP
P178 LMGTPDGPCYPAPPV
Y180 GTPDGPCYPAPPVPR
S190 PPVPRQASPQNL___
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
12662 : Phospho-CAD (Ser1859) Antibody
  rat

 
S10 ALVLEDGSVLQGRPF
K137 EQGSLLGKLVQKGTE
K165 LAPEVSIKTPRVFNA
T166 APEVSIKTPRVFNAG
A172 KTPRVFNAGGAPRIF
K186 FAVDCGLKYNQIRCL
K186 FAVDCGLKYNQIRCL
Y267 LAIGAKTYKMRYGNR
K424 SQAIKALKEENIQTL
T456 KVYFLPITPHYVTQV
T475 RPDGILLTFGGQTAL
T504 YGVRVLGTPVETIEL
T585 VAPAFAHTSQVLIDK
S586 APAFAHTSQVLIDKS
K592 TSQVLIDKSLKGWKE
K696 RSSALASKATGYPLA
Y735 AFEPSLDYCVVKIPR
K739 SLDYCVVKIPRWDLS
K747-ac IPRWDLSkFLRVSTK
K747 IPRWDLSKFLRVSTK
K754 kFLRVSTKIGSCMKS
K760 TKIGSCMKSVGEVMG
S761 KIGSCMKSVGEVMGI
K778 SFEEAFQKALRMVDE
K867 QDLLHQAKCLGFSDK
T883 IALAVLSTELVVRKL
T978 MVNYNPETVSTDYDM
S980 NYNPETVSTDYDMCD
T981 YNPETVSTDYDMCDR
Y983 PETVSTDYDMCDRLY
T1037 QQCRVLGTSPEAIDS
S1038 QCRVLGTSPEAIDSA
S1044 TSPEAIDSAENRFKF
S1061 LLDTIGISQPQWREL
K1119 SSAAAVSKEHPVVIS
K1127 EHPVVISKFIQEAKE
K1211 IAKDDQLKVIECNVR
S1220 IECNVRVSRSFPFVS
S1222 CNVRVSRSFPFVSKT
K1228 RSFPFVSKTLGVDLV
T1239 VDLVALATRIIMGEK
K1262 GSGVVGVKVPQFSFS
S1267 GVKVPQFSFSRLAGA
K1309 AMLSTGFKIPKKNIL
K1313 TGFKIPKKNILLTIG
K1323 LLTIGSYKNKSELLP
K1325 TIGSYKNKSELLPTV
N1387 ILDQLAENHFELVIN
S1406-p GAGGRRLsSFVTKGY
S1407 AGGRRLsSFVTKGYR
K1411 RLsSFVTKGYRTRRL
K1411 RLsSFVTKGYRTRRL
S1423 RRLAADFSVPLIIDI
K1431 VPLIIDIKCTKLFVE
K1434 IIDIKCTKLFVEALG
K1450 IGPAPPLKVHVDCMT
K1460 VDCMTSQKLVRLPGL
K1521 PALALAQKLAEAGAR
K1618 HICHVARKEEILLIK
K1625 KEEILLIKTAKAQGL
K1657 LERLGPGKGEVRPEL
S1724 AVSEGRLSLDDLLQR
Y1794 VLRGEVAYIDGQVLV
Y1805 QVLVPPGYGQDVRKW
P1823 AVPQPPPPTPTTTEI
T1827 PPPPTPTTTEITTTP
T1828 PPPTPTTTEITTTPE
T1831 TPTTTEITTTPERPR
T1832 PTTTEITTTPERPRR
T1833 TTTEITTTPERPRRV
H1856 FHLPPRIHRAsDPGL
S1859-p PPRIHRAsDPGLPAE
T1884 AESELIGTPDGPCYP
P1888 LIGTPDGPCYPVPPV
Y1890 GTPDGPCYPVPPVPR
S1900 PPVPRQASPQNLGSS
S1906 ASPQNLGSSGLLHPQ
M1914 SGLLHPQMSPLLHSL
S1938 QFTKDQMSHLFNVAH
K1961 ERSLDILKGKVMASM
S2089 KHGRTVHSLACLLTQ
Y2103-p QYRVSLRyVAPPSLR
S2108 LRyVAPPSLRMPSSV
S2114 PSLRMPSSVWDFVAS
K2125 FVASRGTKQEEFESI
S2131 TKQEEFESIEEALPD
S2154 IQKERFGSSQEYEAC
S2155 QKERFGSSQEYEACF
Y2158 RFGSSQEYEACFGQF
Y2203 DSDPRAAYFRQAENG
Y2212 RQAENGMYIRMALLA
T2220 IRMALLATVLGRF__
12662 : Phospho-CAD (Ser1859) Antibody
  hamster

 
S10 ALVLEDGSVLQGRPF
K137 EQGSLLGKLVQSGTE
K165 LAPEVSIKTPRVFNA
T166 APEVSIKTPRVFNAG
A172 KTPRVFNAGGAPRIC
K186 CALDCGLKYNQIRCL
K186 CALDCGLKYNQIRCL
Y267 LAIGAKTYKMRYGNR
K424 SQAIKALKEENIQTL
T456-p KVYFLPItPHYVTQV
T475 RPDGVLLTFGGQTAL
T504 YGVRVLGTPVETIEL
T585 VAPAFAHTSQVLIDK
S586 APAFAHTSQVLIDKS
K592 TSQVLIDKSLKGWKE
K696 RSSALASKATGYPLA
Y735 AFEPSLDYCVVKIPR
K739 SLDYCVVKIPRWDLS
K747 IPRWDLSKFLRVSTK
K747 IPRWDLSKFLRVSTK
K754 KFLRVSTKIGSCMKS
K760 TKIGSCMKSVGEVMG
S761 KIGSCMKSVGEVMGI
K778 SFEEAFQKALRMVDE
K867 QDLLHQAKCLGFSDK
T883 IALAVLSTELAVRKL
T978 MVNYNPETVSTDYDM
S980 NYNPETVSTDYDMCD
T981 YNPETVSTDYDMCDR
Y983 PETVSTDYDMCDRLY
T1037-p QQCRVLGtSPEAIDS
S1038 QCRVLGtSPEAIDSA
S1044 tSPEAIDSAENRFKF
S1061 LLDTIGISQPQWREL
K1119 SSAAAVSKEHPVVIS
K1127 EHPVVISKFIQEAKE
K1211 IAKDDQLKVIECNVR
S1220 IECNVRVSRSFPFVS
S1222 CNVRVSRSFPFVSKT
K1228 RSFPFVSKTLGVDLV
T1239 VDLVALATRIIMGEK
K1262 GSGVVGVKVPQFSFS
S1267 GVKVPQFSFSRLAGA
K1309 AMLSTGFKIPKKNIL
K1313 TGFKIPKKNILLTIG
K1323 LLTIGSYKNKSELLP
K1325 TIGSYKNKSELLPTV
N1387 ILDQLAENHFELVIN
S1406-p GAGGRRLsSFVTKGY
S1407 AGGRRLsSFVTKGYR
K1411 RLsSFVTKGYRTRRL
K1411 RLsSFVTKGYRTRRL
S1423 RRLAADFSVPLIIDI
K1431 VPLIIDIKCTKLFVE
K1434 IIDIKCTKLFVEALG
K1450 IGPAPPLKVHVDCMT
K1460 VDCMTSQKLVRLPGL
K1521 PALALAQKLAEAGAR
K1618 HICHVARKEEILLIK
K1625 KEEILLIKTAKAQGL
R1657 LERLGPGRGEVRPEL
S1724 AVSEGRLSLDDLLQR
Y1794 VLRGEVAYIDGQVLV
Y1805 QVLVPPGYGQDVRKW
S1823 AVPQPPPSAPATTEI
T1827 PPPSAPATTEITTTP
T1828 PPSAPATTEITTTPE
T1831 APATTEITTTPERPR
T1832 PATTEITTTPERPRR
T1833 ATTEITTTPERPRRV
H1856 FHLPPRIHRASDPGL
S1859 PPRIHRASDPGLPAE
T1884 VEPELMGTPDGPCYP
P1888 LMGTPDGPCYPAPPV
Y1890 GTPDGPCYPAPPVPR
S1900 PPVPRQASPQNLGSS
S1906 ASPQNLGSSGLLHPQ
T1914 SGLLHPQTSPLLHSL
S1938 QFTKDQMSHLFNVAH
K1961 ERSLDILKGKVMASM
S2089 KHGRTVHSLACLLTQ
Y2103 QYRVSLRYVAPPSLR
S2108 LRYVAPPSLRMPPSV
S2114 PSLRMPPSVWDFVAS
K2125 FVASRGTKQEEFESI
S2131 TKQEEFESIEEALPD
S2154 IQKERFGSTQEYEAC
T2155 QKERFGSTQEYEACF
Y2158 RFGSTQEYEACFGQF
Y2203 DSDPRAAYFRQAENG
Y2212 RQAENGMYIRMALLA
T2220 IRMALLATVLGRF__
12662 : Phospho-CAD (Ser1859) Antibody
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