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Protein Page:
TCF4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
TCF4 Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'. Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin. Interacts with HIVEP2. Interacts with NEUROD2. Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor
Cellular Component: transcription factor complex; nucleus
Molecular Function: protein C-terminus binding; protein binding; DNA binding; protein heterodimerization activity; transcription factor activity
Biological Process: transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transcription initiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; protein-DNA complex assembly
Reference #:  P15884 (UniProtKB)
Alt. Names/Synonyms: BHLHB19; Class B basic helix-loop-helix protein 19; E2-2; Immunoglobulin transcription factor 2; ITF-2; ITF2; MGC149723; MGC149724; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SL3-3 enhancer factor 2; TCF-4; TCF4; Transcription factor 4
Gene Symbols: TCF4
Molecular weight: 71,308 Da
Basal Isoelectric point: 6.51  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  Wnt/├č-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

TCF4

Protein Structure Not Found.


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Sites Implicated In
transcription, induced: S56‑p
molecular association, regulation: S56‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S23 LSDLLDFSAMFSPPV
0 1 S31 AMFSPPVSSGKNGPT
1 0 S54-p GSNVEDRsssGSWGN
1 0 S55-p SNVEDRsssGSWGNG
1 0 S56-p NVEDRsssGSWGNGG
0 1 N61 sssGSWGNGGHPsPs
0 2 S66-p WGNGGHPsPsRNYGD
0 1 S68-p NGGHPsPsRNYGDGT
0 1 Y71 HPsPsRNYGDGTPYD
0 2 S87-p MTSRDLGsHDNLsPP
0 2 S92-p LGsHDNLsPPFVNSR
0 1 Y149-p TKPGSQYyQYSSNNP
0 4 T297-p HYSTSSCtPPANGTD
0 2 S318-p GSGAAGSsQTGDALG
0 1 S340 SPDHTNNSFSSNPST
0 1 S356 VGSPPSLSAGTAVWS
0 1 S370 SRNGGQASSsPNYEG
0 4 S372-p NGGQASSsPNYEGPL
0 3 S484 LPVQSATSPDLNPPQ
0 6 S515-p SGSSEIKsDDEGDEN
0 1 S543 DDKKDIKSITSNNDD
0 2 - gap
0 2 T554-p NNDDEDLtPEQKAER
0 1 K585 RDINEAFKELGRMVQ
0 1 K585-ub RDINEAFkELGRMVQ
0 3 K629-ub RERNLNPkAACLKRR
  TCF4 iso2  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
T137 HYSTSSCTPPANGTD
S158 GSGAAGSSQTGDALG
S180 SPDHTNNSFSSNPST
S196 VGSPPSLSAGTAVWS
S210 SRNGGQASSSPNYEG
S212 NGGQASSSPNYEGPL
S324 LPVQSATSPDLNPPQ
S355 SGSSEIKSDDEGDEN
S383-p DDKKDIKsITRSRsS
S389-p KsITRSRsSNNDDED
T398 NNDDEDLTPEQKAER
K429 RDINEAFKELGRMVQ
K429 RDINEAFKELGRMVQ
K473 RERNLNPKAACLKRR
  mouse

► Hide Isoforms
 
S23-p LSDLLDFsAMFSPPV
S31-p AMFSPPVsSGKNGPT
S54 GSNVEDRSSSGSWGt
S55 SNVEDRSSSGSWGtG
S56 NVEDRSSSGSWGtGG
T61-p SSSGSWGtGGHPsPS
S66-p WGtGGHPsPSRNyGD
S68 tGGHPsPSRNyGDGT
Y71-p HPsPSRNyGDGTPYD
S87-p MTSRDLGsHDNLsPP
S92-p LGsHDNLsPPFVNSR
Y148 TKPGSQYYQYSSNNA
T296 HYSTSSCTPPANGTD
S317 GTGAAGSSQTGDALG
S339-p SPDHTNNsFSSNPST
S355-p VGSPPSLsAGTAVWS
S369-p SRNGGQAsSsPNYEG
S371-p NGGQAsSsPNYEGPL
S483-p LPVQSATsPDLNPPQ
S514-p SGSSEIKsDDEGDEN
S542 DDKKDIKSITRSRSS
S548 KSITRSRSSNNDDED
T557-p NNDDEDLtPEQKAER
K588-ac RDINEAFkELGRMVQ
K588 RDINEAFKELGRMVQ
K632 RERNLNPKAACLKRR
  TCF4 iso3  
S23 LSDLLDFSAMFSPPV
S31 AMFSPPVSSGKNGPT
S54 GSNVEDRSSSGSWGT
S55 SNVEDRSSSGSWGTG
S56 NVEDRSSSGSWGTGG
T61 SSSGSWGTGGHPSPS
S66 WGTGGHPSPSRNYGD
S68 TGGHPSPSRNYGDGT
Y71 HPSPSRNYGDGTPYD
S87 MTSRDLGSHDNLSPP
S92 LGSHDNLSPPFVNSR
Y148 TKPGSQYYQYSSNNA
T296 HYSTSSCTPPANGTD
S317 GTGAAGSSQTGDALG
S339 SPDHTNNSFSSNPST
S355 VGSPPSLSAGTAVWS
S369 SRNGGQASSSPNYEG
S371 NGGQASSSPNYEGPL
S483 LPVQSATSPDLNPPQ
S514 SGSSEIKSDDEGDEN
S542 DDKKDIKSITSNNDD
- gap
T553-p NNDDEDLtPEQKAER
K584 RDINEAFKELGRMVQ
K584 RDINEAFKELGRMVQ
K628 RERNLNPKAACLKRR
  rat

 
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S8 MTSRDLGSHDNLSPP
S13 LGSHDNLSPPFANSR
Y68 TKPGSQYYPYSSNNA
T215 HYSTSSCTPPANGTD
S236 GTGAAGSSQTGDALG
S258 SPDHTNNSFSSNPST
S274 VGSPPSLSAGTAVWS
S288 SRNGGQASSSPNYEG
S290 NGGQASSSPNYEGPL
S402 LPVQSATSPDLNPPQ
S433 SGSSEIKSDDEGDEN
S461 DDKKDIKSITRSRSS
S467 KSITRSRSSNNDDED
T476 NNDDEDLTPEQKAER
K507 RDINEAFKELGRMVQ
K507 RDINEAFKELGRMVQ
K551 RERNLNPKAACLKRR
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