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Protein Page:
Calpain-2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Calpain-2 Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Ubiquitous. Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin. Belongs to the peptidase C2 family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; Protease; EC 3.4.22.53
Cellular Component: Golgi apparatus; cortical actin cytoskeleton; endoplasmic reticulum; cytoplasm; dendrite; plasma membrane; pseudopodium; nucleus; chromatin; cytosol; lipid raft
Molecular Function: protein binding; protein heterodimerization activity; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; cysteine-type peptidase activity
Biological Process: protein autoprocessing; blastocyst development; proteolysis involved in cellular protein catabolic process; response to hypoxia; proteolysis; myoblast fusion; regulation of cytoskeleton organization and biogenesis
Reference #:  P17655 (UniProtKB)
Alt. Names/Synonyms: Calcium-activated neutral proteinase 2; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; Calpain large polypeptide L2; Calpain M-type; calpain, large polypeptide L2; Calpain-2; Calpain-2 catalytic subunit; Calpain-2 large subunit; CAN2; CANP 2; CANP2; CANPL2; CANPml; CAPN2; FLJ39928; M-calpain; mCANP; Millimolar-calpain
Gene Symbols: CAPN2
Molecular weight: 79,995 Da
Basal Isoelectric point: 4.87  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Calpain-2

Protein Structure Not Found.


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Sites Implicated In
cell adhesion, altered: S50‑p
cell motility, altered: S50‑p, S369‑p, T370‑p
activity, inhibited: S369‑p, T370‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K7-ac _MAGIAAkLAKDREA
0 1 K26-ub GSHDRAIkYLNQDyE
0 1 Y32-p IkYLNQDyEALRNEC
2 0 S50-p GTLFQDPsFPAIPSA
0 4 K61-ac IPSALGFkELGPYSS
0 1 K69-ub ELGPYSSkTRGIEWK
0 3 K260-ub ITFQKLVkGHAYSVT
0 2 K280-ub ESNGSLQkLIRIRNP
0 1 Y353-p DTLTSDTykkWKLTk
0 19 K354-ac TLTSDTykkWKLTkM
0 5 K355-ac LTSDTykkWKLTkMD
0 1 K360-ub ykkWKLTkMDGNWRR
1 2 S369-p DGNWRRGstAGGCRN
2 0 T370-p GNWRRGstAGGCRNY
0 1 Y377 tAGGCRNYPNTFWMN
1 0 K390-sm MNPQYLIkLEEEDED
0 1 S403-p EDEEDGEsGCTFLVG
0 1 T464-p RARERSDtFINLREV
0 1 K476-ub REVLNRFkLPPGEYI
0 1 S569-p AKRQDIKsDGFSIET
0 1 S586-p IMVDMLDsDGsGKLG
0 2 S589-p DMLDsDGsGKLGLKE
0 1 Y611-p IQKYQKIyREIDVDR
0 1 T621-p IDVDRSGtMNSyEMR
0 1 Y625-p RSGtMNSyEMRKALE
0 3 K674-ub VRLETLFkIFKQLDP
  mouse

 
K7 _MAGIAIKLAKDREA
K26 GSHERAIKYLNQDYE
Y32 IKYLNQDYETLRNEC
S50-p GALFQDPsFPALPSS
K61 LPSSLGYKELGPYSS
K69 ELGPYSSKTRGIEWK
K260-ub VTYQKLVkGHAYSVT
K280-ub ESSGSLQkLIRIRNP
Y353 DTLTCDSYkkWKLTK
K354-ac TLTCDSYkkWKLTKM
K355-ac LTCDSYkkWKLTKMD
K360 YkkWKLTKMDGNWRR
S369-p DGNWRRGstAGGCRN
T370-p GNWRRGstAGGCRNy
Y377-p tAGGCRNyPNTFWMN
K390 MNPQYLIKLEEEDED
R403 EDEEDGERGCTFLVG
T464 RARERSDTFINLREV
K476 REVLNRFKLPPGEYV
S569 AKRQDIKSDGFSIET
E586 IMVDMLDEDGSGKLG
S589 DMLDEDGSGKLGLKE
Y611 IQKYQKIYREIDVDR
T621 IDVDRSGTMNSYEMR
Y625 RSGTMNSYEMRKALE
K674-ub VRLETLFkIFKQLDP
  rat

 
K7 _MAGIAMKLAKDREA
K26 GSHERAIKYLNQDYE
Y32 IKYLNQDYETLRNEC
S50 GALFQDPSFPALPSS
K61 LPSSLGFKELGPYSS
K69 ELGPYSSKTRGIEWK
K260 VTYQKLVKGHAYSVT
K280 ESSGSLQKLIRIRNP
Y353 DTLTCDSYKKWKLTK
K354 TLTCDSYKKWKLTKM
K355 LTCDSYKKWKLTKMD
K360 YKKWKLTKMDGNWRR
S369 DGNWRRGSTAGGCRN
T370 GNWRRGSTAGGCRNY
Y377 TAGGCRNYPNTFWMN
K390 MNPQYLIKLEEEDED
R403 EDDEDGERGCTFLVG
T464 RARERSDTFINLREV
K476 REVLNRFKLPPGEYV
S569 AKREDIKSDGFSIET
E586 IMVDMLDEDGSGKLG
S589 DMLDEDGSGKLGLKE
Y611 IQKYQKIYREIDVDR
T621 IDVDRSGTMNSYEMR
Y625 RSGTMNSYEMRKALE
K674 VRLEILFKIFKQLDP
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