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Fe65 (human)

Overview Fe65 adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on Y142 (H2AXpY142) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as TIP60, probably explains its trancription activation activity. Note: This description may include information from UniProtKB. Protein type: Transcription regulation; Apoptosis; Adaptor/scaffold Cellular Component: presynaptic membrane; postsynaptic membrane; growth cone; protein complex; perinuclear region of cytoplasm; lamellipodium; cytoplasm; dendritic spine; plasma membrane; nuclear speck; synapse; nucleus Molecular Function: protein binding; histone binding; beta-amyloid binding; chromatin binding; transcription factor binding Biological Process: axon guidance; extracellular matrix organization and biogenesis; positive regulation of DNA repair; transcription, DNA-dependent; negative regulation of S phase of mitotic cell cycle; positive regulation of apoptosis; apoptosis; positive regulation of transcription, DNA-dependent; neuron migration; signal transduction; negative regulation of thymidylate synthase biosynthetic process; negative regulation of neuron differentiation; positive regulation of protein secretion; regulation of transcription, DNA-dependent; axonogenesis; double-strand break repair; visual learning; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; cell cycle arrest; response to iron ion; response to DNA damage stimulus Reference #:  O00213 (UniProtKB) Alt. Names/Synonyms: adaptor protein FE65a2; amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65); Amyloid beta A4 precursor protein-binding family B member 1; amyloid beta A4 precursor protein-binding, family B, member 1; APBB1; FE65; MGC:9072; Protein Fe65; RIR; stat-like protein Gene Symbols: APBB1 Molecular weight: 77,244 Da Basal Isoelectric point: 4.98  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below Fe65 2E45 - A=237-290 (human) 2HO2 - A=253-289 (human) 2IDH - A/B/C/D/E/F/G/H=253-289 (human) 2OEI - A=253-289 (human) 3D8D - A/B=366-505 (human) 3D8E - A/D/C/B=366-505 (human) 3D8F - A/D/C/B=366-505 (human) 3DXC - A/C=534-667 (human) 3DXD - A/C=534-667 (human) 3DXE - A/C=534-667 (human)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 2E45 2HO2 2IDH 2OEI 3D8D 3D8E 3D8F 3DXC 3DXD 3DXE  |  Phospho3D  |  DISEASE  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene

	Sites Implicated In
transcription, altered: Y547‑p molecular association, regulation: Y547‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human ► Hide Isoforms
0	2		K48-u	QATAVGPkDLRSAMG
0	1		P135	ANRGLRGPGLIISTQ
1	1		S175-p	EEEEDLSsPPGLPEP
1	1		S287-p	WEPPGRAsPSQGSSP
1	2		S347-p	TFPAQSLsPEPLPQE
0	1		S459-p	VWGVGRDsGRERDFA
0	1		S517	RCLVNGLSLDHSKLV
2	1		Y547-p	VQKFQVYyLGNVPVA
1	0		S610	ECRVRFLSFLAVGRD
0	1		S666	QKCLDARSQASTSCL
0	1		S695-p	TVRRGVQsLWGsLKP
0	2		S699-p	GVQsLWGsLKPKRLG
1	2		T709-p	PKRLGAHtP______

	Fe65 iso2
K48	QATAVGPKDLRSAMG
P135	ANRGLRGPGLIISTQ
S175	EEEEDLSSPPGLPEP
S287	WEPPGRASPSQGSSP
S347	TFPAQSLSPEPLPQE
S459	VWGVGRDSGRDFAYV
S515	RCLVNGLSLDHSKLV
Y545	VQKFQVYYLGNVPVA
S608	ECRVRFLSFLAVGRD
S664	QKCLDARSQASTSCL
S693	TVRRGVQSLWGSLKP
S697	GVQSLWGSLKPKRLG
T707	PKRLGAHTP______

	mouse
K48-u	QATAVVPkDLRSAMG
S135-p	ANRGLHGsALIINTQ
S175	EEEEDLSSPPGLPEP
S287	WEPPGRASPSQGSSP
S347-p	SFPAQSLsPEPVPQE
S459	VWGVGRDSGSNRDFA
S517-p	RCLVNGLsLDHSKLV
Y547-p	VQKFQVYyLGNVPVA
S610	ECRVRFLSFLAVGRD
S666-g	QKCLDARsQTSTSCL
S695	TVRRGVQSLWGsLKP
S699-p	GVQSLWGsLKPKRLG
T709-p	PKRLGSQtP______

	rat
K48	QATAVVPKDLRSAMG
S135	ANRGLHGSALIINTQ
S176	EEEEDLSSPQGLPEP
S288	WEPPGRASPSQGNSP
S348	PFSAQSLSPEPVPQE
S460	VWGVGRDSGRERDFA
S518	RCLVNGLSLDHSKLV
Y548	VQKFQVYYLGNVPVA
S611-p	ECRVRFLsFLAVGRD
S667	QKCLDARSQTSTSCL
S696	TVRRGVQSLWGSLKP
S700	GVQSLWGSLKPKRLG
T710	PKRLGSQTP______

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