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Protein Page:
axin 1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
axin 1 is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin-1 functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Wild-type axin 1 can induce apoptosis in hepatocellular and colorectal cancer cells. Is downregulated during progression of esophageal squamous cell carcinoma. Mutation of the axin-1 gene is associated with hepatocellular carcinoma, anaplastic thyroid cancer, medulloblastoma and colorectal cancer. May have a role in oncogenesis in Hodgkin lymphoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold
Cellular Component: Golgi apparatus; cytoplasmic microtubule; perinuclear region of cytoplasm; postsynaptic density; cytoplasmic membrane-bound vesicle; cytoplasm; cell cortex; cytoplasmic vesicle; cytosol; beta-catenin destruction complex; nucleus; lateral plasma membrane
Molecular Function: protein C-terminus binding; identical protein binding; protein homodimerization activity; protein self-association; beta-catenin binding; protein kinase binding; GTPase activator activity; protein binding; signal transducer activity; enzyme binding; ubiquitin protein ligase binding; protein complex scaffold; SMAD binding; receptor binding
Biological Process: cell death; negative regulation of Wnt receptor signaling pathway; protein polyubiquitination; embryonic eye morphogenesis; apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; nucleocytoplasmic transport; negative regulation of protein metabolic process; dorsal/ventral axis specification; Wnt receptor signaling pathway through beta-catenin; olfactory placode formation; activation of JNK activity; cytoplasmic microtubule organization and biogenesis; sensory perception of sound; positive regulation of ubiquitin-protein ligase activity; Wnt receptor signaling pathway involved in forebrain neuron fate commitment; protein catabolic process; protein homooligomerization; axial mesoderm formation; in utero embryonic development; positive regulation of transforming growth factor beta receptor signaling pathway; muscle cell development; negative regulation of fat cell differentiation; positive regulation of peptidyl-serine phosphorylation; optic placode formation; cellular protein complex assembly; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; forebrain anterior/posterior pattern formation; activation of protein kinase activity; positive regulation of protein catabolic process; positive regulation of protein amino acid phosphorylation; determination of left/right symmetry; positive regulation of GTPase activity
Reference #:  O15169 (UniProtKB)
Alt. Names/Synonyms: AXIN; axin 1; Axin-1; AXIN1; Axis inhibition protein 1; axis inhibitor 1; AXN1; fused, mouse, homolog of; hAxin; MGC52315
Gene Symbols: AXIN1
Molecular weight: 95,635 Da
Basal Isoelectric point: 6.5  Predict pI for various phosphorylation states
CST Pathways:  ESC Pluripotency and Differentiation  |  Wnt/├č-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

axin 1

Protein Structure Not Found.


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Sites Implicated In
transcription, inhibited: S75‑p, S77‑p, S217‑p, S469‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 5 T60-p KGETSTAtPRRSDLD
1 26 S75-p LGYEPEGsAsPtPPY
1 15 S77-p YEPEGsAsPtPPYLK
0 14 T79-p PEGsAsPtPPYLKWA
0 1 Y148-p ARAIYRKyILDNNGI
1 1 S157 LDNNGIVSRQtKPAT
0 1 T160-p NGIVSRQtKPATKSF
1 3 S217-p YTRTGSEsPKVCSDQ
0 1 S282 TAAPRVSSSRRYSEG
0 1 S283 AAPRVSSSRRYSEGR
1 0 S317 YALAPATSANDSEQQ
1 0 S321 PATSANDSEQQSLSS
1 0 S325 ANDSEQQSLSSDADT
1 2 S469-p AHEENPEsILDEHVQ
4 1 T481-p HVQRVLRtPGRQsPG
2 7 S486-p LRtPGRQsPGPGHRs
2 9 S493-p sPGPGHRsPDSGHVA
0 1 S511-p VALGGAAsGHGKHVP
0 1 K519 GHGKHVPKSGAKLDA
0 1 S581-p RSRGYSEsVGAAPNA
0 1 S776 QRKVGGGSAQPCDSI
0 1 S782 GSAQPCDSIVVAYYF
0 2 Y796-p FCGEPIPyRTLVRGR
2 0 K857 FEEKIIGKVEKVD__
2 0 K860 KIIGKVEKVD_____
  mouse

 
T60 KSETSTATPRRSDLD
S75-p LGYEPEGsAsPtPPY
S77-p YEPEGsAsPtPPYLR
T79-p PEGsAsPtPPYLRWA
Y148 ARAIYRKYILDSNGI
S157-p LDSNGIVsRQTKPAT
T160 NGIVsRQTKPATKSF
S217-p YTRTGSEsPKVCSDQ
S282 TAAPRAPSSRRYNEG
S283 AAPRAPSSRRYNEGR
S317 YALAPATSANDSEQQ
S321 PATSANDSEQQSLSS
S325 ANDSEQQSLSSDADT
S468 AHEENPESILDEHVQ
T480-p HVQRVMRtPGCQsPG
S485-p MRtPGCQsPGPGHRs
S492-p sPGPGHRsPDSGHVA
S509 AVLGGTASGHGKHVP
K517-a GHGKHVPkLGLKLDT
N578 KPRSYSENAGTTLSA
S777-p QRKAGGGsAPPCDsI
S783-p GsAPPCDsIVVAYYF
Y797-p FCGEPIPyRTLVRGR
K858-s FEEKIIGkVEkVD__
K861-s KIIGkVEkVD_____
  rat

 
T60 KSETSTATPRRSDLD
S75 LGYEPEGSASPTPPY
S77 YEPEGSASPTPPYLR
T79 PEGSASPTPPYLRWA
Y148 ARAIYRKYILDSNGI
S157 LDSNGIVSRQtKPAT
T160-p NGIVSRQtKPATKSF
S217 YTRTGSESPKVCSDQ
S282-p TAAPRAPssRRYNEG
S283-p AAPRAPssRRYNEGR
S317-p YALAPATsANDsEQQ
S321-p PATsANDsEQQsLSS
S325-p ANDsEQQsLSSDADT
S468 AHEENPESILDEHVQ
T480-p HVQRVMRtPGCQsPG
S485-p MRtPGCQsPGPGHRs
S492-p sPGPGHRsPDSGHVA
S509 AVLGGTASGHGKHAP
K517 GHGKHAPKLGLKLDS
S578 KPRSYSESTGTNPSA
S741 QRKAGGGSAPPCDSI
S747 GSAPPCDSIVVAYYF
Y761 FCGEPIPYRTLVRGR
K822 FEEKIIGKVEKVD__
K825 KIIGKVEKVD_____
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