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Protein Page:
axin 1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
axin 1 is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin-1 functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Wild-type axin 1 can induce apoptosis in hepatocellular and colorectal cancer cells. Is downregulated during progression of esophageal squamous cell carcinoma. Mutation of the axin-1 gene is associated with hepatocellular carcinoma, anaplastic thyroid cancer, medulloblastoma and colorectal cancer. May have a role in oncogenesis in Hodgkin lymphoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; cytoplasmic microtubule; cytoplasm; cytoplasmic membrane-bound vesicle; postsynaptic density; cytoplasmic vesicle; cell cortex; beta-catenin destruction complex; nucleus; cytosol; lateral plasma membrane
Molecular Function: protein C-terminus binding; identical protein binding; protein homodimerization activity; protein self-association; p53 binding; beta-catenin binding; protein kinase binding; GTPase activator activity; protein binding; signal transducer activity; enzyme binding; receptor signaling complex scaffold activity; ubiquitin protein ligase binding; protein complex scaffold; SMAD binding; receptor binding
Biological Process: cell death; protein polyubiquitination; negative regulation of Wnt receptor signaling pathway; embryonic eye morphogenesis; apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; negative regulation of protein metabolic process; nucleocytoplasmic transport; dorsal/ventral axis specification; Wnt receptor signaling pathway through beta-catenin; olfactory placode formation; cytoplasmic microtubule organization and biogenesis; activation of JNK activity; sensory perception of sound; positive regulation of ubiquitin-protein ligase activity; Wnt receptor signaling pathway involved in forebrain neuron fate commitment; protein catabolic process; protein homooligomerization; axial mesoderm formation; in utero embryonic development; positive regulation of transforming growth factor beta receptor signaling pathway; muscle cell development; negative regulation of fat cell differentiation; optic placode formation; positive regulation of peptidyl-serine phosphorylation; cellular protein complex assembly; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; forebrain anterior/posterior pattern formation; activation of protein kinase activity; positive regulation of protein catabolic process; positive regulation of protein amino acid phosphorylation; determination of left/right symmetry; positive regulation of GTPase activity
Disease: Caudal Duplication Anomaly; Hepatocellular Carcinoma
Reference #:  O15169 (UniProtKB)
Alt. Names/Synonyms: AXIN; axin 1; Axin-1; AXIN1; Axis inhibition protein 1; axis inhibitor 1; AXN1; fused, mouse, homolog of; hAxin; MGC52315
Gene Symbols: AXIN1
Molecular weight: 95,635 Da
Basal Isoelectric point: 6.5  Predict pI for various phosphorylation states
CST Pathways:  ESC Pluripotency and Differentiation  |  Wnt/├č-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

axin 1

Protein Structure Not Found.


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Sites Implicated In
transcription, inhibited: S75‑p, S77‑p, S217‑p, S469‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 5 T60-p KGETSTAtPRRSDLD
1 28 S75-p LGYEPEGsAsPtPPy
1 18 S77-p YEPEGsAsPtPPyLK
0 18 T79-p PEGsAsPtPPyLKWA
0 1 Y82-p sAsPtPPyLKWAESL
0 1 Y148-p ARAIYRKyILDNNGI
1 1 S157 LDNNGIVSRQtKPAT
1 2 T160-p NGIVSRQtKPATKSF
0 1 T164 SRQtKPATKSFIKGC
0 1 S166 QtKPATKSFIKGCIM
1 3 S217-p YTRTGSEsPKVCSDQ
0 1 S282 TAAPRVSSSRRYSEG
0 1 S283 AAPRVSSSRRYSEGR
1 0 S317 YALAPATSANDSEQQ
1 0 S321 PATSANDSEQQSLSS
1 0 S325 ANDSEQQSLSSDADT
1 2 S469-p AHEENPEsILDEHVQ
4 3 T481-p HVQRVLRtPGRQsPG
2 8 S486-p LRtPGRQsPGPGHRs
2 11 S493-p sPGPGHRsPDSGHVA
0 2 S511-p VALGGAAsGHGKHVP
0 1 K519 GHGKHVPKSGAKLDA
0 2 S581-p RSRGYSEsVGAAPNA
0 1 S611-p RNAKKAEsGKsAstE
0 1 S614-p KKAEsGKsAstEVPG
0 1 S616-p AEsGKsAstEVPGAs
0 1 T617-p EsGKsAstEVPGAsE
0 1 S623-p stEVPGAsEDAEKNQ
0 1 S776 QRKVGGGSAQPCDSI
0 1 S782 GSAQPCDSIVVAyyF
0 1 Y787-p CDSIVVAyyFCGEPI
0 1 Y788-p DSIVVAyyFCGEPIP
0 2 Y796-p FCGEPIPyRTLVRGR
0 1 Y822 TKKGSYRYYFKKVSD
1 0 K825 GSYRYYFKKVSDEFD
2 0 K857 FEEKIIGKVEKVD__
1 0 K857 FEEKIIGKVEKVD__
2 0 K860 KIIGKVEKVD_____
  axin 1 iso1  
T60 KGETSTATPRRSDLD
S75 LGYEPEGSASPTPPY
S77 YEPEGSASPTPPYLK
T79 PEGSASPTPPYLKWA
Y82 SASPTPPYLKWAESL
Y148 ARAIYRKYILDNNGI
S157 LDNNGIVSRQTKPAT
T160 NGIVSRQTKPATKSF
T164 SRQTKPATKSFIKGC
S166 QTKPATKSFIKGCIM
S217 YTRTGSESPKVCSDQ
S282 TAAPRVSSSRRYSEG
S283 AAPRVSSSRRYSEGR
S317 YALAPATSANDSEQQ
S321 PATSANDSEQQSLSS
S325 ANDSEQQSLSSDADT
S469 AHEENPESILDEHVQ
T481 HVQRVLRTPGRQSPG
S486 LRTPGRQSPGPGHRS
S493 SPGPGHRSPDSGHVA
S511 VALGGAASGHGKHVP
K519 GHGKHVPKSGAKLDA
- under review  
S611 RNAKKAESGKSASTE
S614 KKAESGKSASTEVPG
S616 AESGKSASTEVPGAS
T617 ESGKSASTEVPGASE
S623 STEVPGASEDAEKNQ
S740 QRKVGGGSAQPCDSI
S746 GSAQPCDSIVVAYYF
Y751 CDSIVVAYYFCGEPI
Y752 DSIVVAYYFCGEPIP
Y760 FCGEPIPYRTLVRGR
Y786 TKKGSYRYYFkKVSD
K789-ub GSYRYYFkKVSDEFD
K821 FEEKIIGKVEKVD__
K821-ub FEEKIIGkVEKVD__
K824 KIIGkVEKVD_____
  mouse

 
T60 KSETSTATPRRSDLD
S75-p LGYEPEGsAsPtPPY
S77-p YEPEGsAsPtPPYLR
T79-p PEGsAsPtPPYLRWA
Y82 sAsPtPPYLRWAESL
Y148 ARAIYRKYILDSNGI
S157-p LDSNGIVsRQtKPAT
T160-p NGIVsRQtKPATKSF
T164 sRQtKPATKSFIKDC
S166 QtKPATKSFIKDCVM
S217-p YTRTGSEsPKVCSDQ
S282 TAAPRAPSSRRYNEG
S283 AAPRAPSSRRYNEGR
S317 YALAPATSANDSEQQ
S321 PATSANDSEQQSLSS
S325 ANDSEQQSLSSDADT
S468 AHEENPESILDEHVQ
T480-p HVQRVMRtPGCQsPG
S485-p MRtPGCQsPGPGHRs
S492-p sPGPGHRsPDSGHVA
S509 AVLGGTASGHGKHVP
K517-ac GHGKHVPkLGLKLDT
N578 KPRSYSENAGTTLSA
S607 RNTKKAESGKNANAE
N610 KKAESGKNANAEVPS
N612 AESGKNANAEVPSTT
A613 ESGKNANAEVPSTTE
T619 NAEVPSTTEDAEKNQ
S777-p QRKAGGGsAPPCDsI
S783-p GsAPPCDsIVVAYYF
Y788 CDsIVVAYYFCGEPI
Y789 DsIVVAYYFCGEPIP
Y797-p FCGEPIPyRTLVRGR
Y823 TKKGSYRYYFKKVSD
K826 GSYRYYFKKVSDEFD
K858-sm FEEKIIGkVEkVD__
K858 FEEKIIGKVEkVD__
K861-sm KIIGkVEkVD_____
  rat

 
T60 KSETSTATPRRSDLD
S75 LGYEPEGSASPTPPY
S77 YEPEGSASPTPPYLR
T79 PEGSASPTPPYLRWA
Y82 SASPTPPYLRWAESL
Y148 ARAIYRKYILDSNGI
S157 LDSNGIVSRQtKPAt
T160-p NGIVSRQtKPAtKsF
T164-p SRQtKPAtKsFIKDC
S166-p QtKPAtKsFIKDCVM
S217 YTRTGSESPKVCSDQ
S282-p TAAPRAPssRRYNEG
S283-p AAPRAPssRRYNEGR
S317-p YALAPATsANDsEQQ
S321-p PATsANDsEQQsLSS
S325-p ANDsEQQsLSSDADT
S468 AHEENPESILDEHVQ
T480-p HVQRVMRtPGCQsPG
S485-p MRtPGCQsPGPGHRs
S492-p sPGPGHRsPDSGHVA
S509 AVLGGTASGHGKHAP
K517 GHGKHAPKLGLKLDS
S578 KPRSYSESTGTNPSA
S607 RNTKKAESGKNASAE
N610 KKAESGKNASAEVPS
S612 AESGKNASAEVPSTT
A613 ESGKNASAEVPSTTE
T619 SAEVPSTTEDAEKNQ
S741 QRKAGGGSAPPCDSI
S747 GSAPPCDSIVVAYYF
Y752 CDSIVVAYYFCGEPI
Y753 DSIVVAYYFCGEPIP
Y761 FCGEPIPYRTLVRGR
Y787-p TKKGSYRyYFKKVSD
K790 GSYRyYFKKVSDEFD
K822 FEEKIIGKVEKVD__
K822 FEEKIIGKVEKVD__
K825 KIIGKVEKVD_____
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