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Protein Page:
GTF2I (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
GTF2I a multifunctional phosphoprotein with roles in transcription and signal transduction. Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and Phox1 and interacts cooperatively with Phox1 to promote serum-inducible transcription of a reporter gene driven by the C- FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Its gene is known to contain at least 34 exons. Four splice variant isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Transcription, coactivator/corepressor; DNA binding protein
Cellular Component: cell projection; cell soma; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; DNA binding; mitogen-activated protein kinase binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of angiogenesis; embryonic development; signal transduction
Reference #:  P78347 (UniProtKB)
Alt. Names/Synonyms: BAP-135; BAP135; Bruton tyrosine kinase-associated protein 135; BTK-associated protein 135; BTK-associated protein, 135kD; BTKAP1; DIWS; FLJ38776; FLJ56355; General transcription factor II-I; general transcription factor IIi; GTF2I; GTFII-I; IB291; SPIN; SRF-Phox1-interacting protein; TFII-I; WBS; WBSCR6; Williams-Beuren syndrome chromosomal region 6 protein; Williams-Beuren syndrome chromosome region 6
Gene Symbols: GTF2I
Molecular weight: 112,416 Da
Basal Isoelectric point: 6.09  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

GTF2I
Protein Structure Not Found.


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Sites Implicated In
transcription, altered: Y248‑p, Y249‑p, S412‑p, S784‑p
activation: Y248‑p, Y249‑p
intracellular localization: Y248‑p, Y249‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

► Hide Isoforms 		 
0 1 K74-u NTRKDFQkDFVKYCV
0 5 S103-p TTQANRMsVDAVEIE
0 1 K140-u VVPVPYEkMLRDQSA
0 2 K160-u LPEGVAFkHPENYDL
0 1 T202-p VGGRVMVtDADRsIL
0 3 S207-p MVtDADRsILsPGGs
0 9 S210-p DADRsILsPGGsCGP
0 1 S214-p sILsPGGsCGPIKVK
0 1 K219 GGsCGPIKVKTEPTE
0 1 T238-p SLEMAAVtVkEESED
0 1 K240-s EMAAVtVkEESEDPD
8 12 Y248-p EESEDPDyyQYNIQA
5 1 Y249-p ESEDPDyyQYNIQAG
0 3 Y251 EDPDyyQYNIQAGPS
0 1 S319 TIEDDDYSPPSKRPK
0 2 A327 PPSKRPKANELPQPP
0 13 K343-a PEPANAGkRKVREFN
0 7 K353-u VREFNFEkWNARITD
1 3 Y398-p QSHVEDLyVEGLPEG
2 132 S412-p GIPFRRPstYGIPRL
0 6 T413-p IPFRRPstYGIPRLE
0 1 S441 KKHELLNSTREDLQL
0 2 K450-a REDLQLDkPASGVKE
0 6 Y460-p SGVKEEWyARITKLR
0 1 K476-u MVDQLFCkkFAEALG
0 4 K477-u VDQLFCkkFAEALGS
0 5 K488-u ALGSTEAkAVPYQkF
0 1 K494-u AkAVPYQkFEAHPND
1 4 Y503-p EAHPNDLyVEGLPEN
0 11 S515-p PENIPFRsPsWyGIP
0 9 S517-p NIPFRsPsWyGIPRL
0 1 Y519-p PFRsPsWyGIPRLEk
0 2 K526-u yGIPRLEkIIQVGNR
0 1 K539-u NRIKFVIkRPELLTH
1 7 T556-p TEVTQPRtNtPVkED
1 21 T558-p VTQPRtNtPVkEDWN
0 6 K561-a PRtNtPVkEDWNVRI
0 1 K561-u PRtNtPVkEDWNVRI
0 2 S620-p PEGIPFRsPTWFGIP
2 0 Y652 KPELVISYLPPGMAs
0 1 S659-p YLPPGMAsKINtKAL
0 1 T663-p GMAsKINtKALQsPK
1 11 S668-p INtKALQsPKRPRsP
1 29 S674-p QsPKRPRsPGsNsKV
0 9 S677-p KRPRsPGsNsKVPEI
0 12 S679-p PRsPGsNsKVPEIEV
0 4 T687-p KVPEIEVtVEGPNNN
0 4 N695 VEGPNNNNPQTsAVR
0 2 S699-p NNNNPQTsAVRtPtQ
0 3 T703-p PQTsAVRtPtQTNGS
0 3 T705-p TsAVRtPtQTNGSNV
0 42 S722-p KPRGREFsFEAWNAK
0 5 K752-u CGEALGLkQAVKVPF
0 1 Y770-p ESFPEDFyVEGLPEG
2 267 S784-p GVPFRRPstFGIPRL
0 38 T785-p VPFRRPstFGIPRLE
0 1 S818-p FETAIKEsTssKsPP
0 4 S820-p TAIKEsTssKsPPRK
0 3 S821-p AIKEsTssKsPPRKI
0 21 S823-p KEsTssKsPPRKINs
0 3 S830-p sPPRKINssPNVNtt
0 6 S831-p PPRKINssPNVNttA
0 1 T836-p NssPNVNttAsGVED
0 1 T837-p ssPNVNttAsGVEDL
0 1 S839-p PNVNttAsGVEDLNI
0 2 K879-u VNDLFSRkFGEAIGM
0 13 Y920-p VSFKAPSyLEISsMR
0 1 S925-p PSyLEISsMRRILDS
0 1 K991-s TDGSSQIkQEPDPTW
  GTF2I iso2  
K74 NTRKDFQKDFVKYCV
S103 TTQANRMSVDAVEIE
K140 VVPVPYEKMLRDQSA
K160 LPEGVAFKHPENYDL
T202 VGGRVMVTDADRSIL
S207 MVTDADRSILSPGGS
S210 DADRSILSPGGSCGP
S214 SILSPGGSCGPIKVK
K219 GGSCGPIKVKTEPTE
T238 SLEMAAVTVKEESED
K240 EMAAVTVKEESEDPD
Y248-p EESEDPDyyQyNIQG
Y249-p ESEDPDyyQyNIQGS
Y251-p EDPDyyQyNIQGSHH
S278-p PAEDDDYsPPSKRPK
A286 PPSKRPKANELPQPP
K302 PEPANAGKRKVREFN
K312 VREFNFEKWNARITD
Y357-p QSHVEDLyVEGLPEG
S371-p GIPFRRPsTYGIPRL
T372 IPFRRPsTYGIPRLE
S400 KKHELLNSTREDLQL
K409 REDLQLDKPASGVKE
Y419 SGVKEEWYARITKLR
K435 MVDQLFCKKFAEALG
K436 VDQLFCKKFAEALGS
K447 ALGSTEAKAVPYQKF
K453 AKAVPYQKFEAHPND
Y462-p EAHPNDLyVEGLPEN
S474 PENIPFRSPSWYGIP
S476 NIPFRSPSWYGIPRL
Y478 PFRSPSWYGIPRLEK
K485 YGIPRLEKIIQVGNR
K498 NRIKFVIKRPELLTH
T515 TEVTQPRTNTPVKED
T517 VTQPRTNTPVKEDWN
K520 PRTNTPVKEDWNVRI
K520 PRTNTPVKEDWNVRI
S579 PEGIPFRSPTWFGIP
Y611-p KPELVISyLPPGMAS
S618 yLPPGMASKINTKAL
T622 GMASKINTKALQsPK
S627-p INTKALQsPKRPRsP
S633-p QsPKRPRsPGSNSKV
S636 KRPRsPGSNSKVPEI
S638 PRsPGSNSKVPEIEV
T646 KVPEIEVTVEGPNNN
N654 VEGPNNNNPQTSAVR
S658 NNNNPQTSAVRTPTQ
T662 PQTSAVRTPTQTNGS
T664 TSAVRTPTQTNGSNV
S681-p KPRGREFsFEAWNAK
K711 CGEALGLKQAVKVPF
Y729-p ESFPEDFyVEGLPEG
S743-p GVPFRRPstFGIPRL
T744-p VPFRRPstFGIPRLE
S777 FETAIKESTSSKSPP
S779 TAIKESTSSKSPPRK
S780 AIKESTSSKSPPRKI
S782 KESTSSKSPPRKINS
S789 SPPRKINSSPNVNTT
S790 PPRKINSSPNVNTTA
T795 NSSPNVNTTASGVED
T796 SSPNVNTTASGVEDL
S798 PNVNTTASGVEDLNI
K838 VNDLFSRKFGEAIGM
Y879 VSFKAPSYLEISSMR
S884 PSYLEISSMRRILDS
K950 TDGSSQIKQEPDPTW
  mouse

► Hide Isoforms 		 
K74 NTRKDFQKDFVKYCV
S103-p TTQANRMsVDAVEIE
K140 VVPVPYEKMLRDQSA
K160 LPEGVAFKHPEHYDL
A202 LGGRVLAAEAERSML
S207 LAAEAERSMLSPSGS
S210 EAERSMLSPSGSCGP
S214 SMLSPSGSCGPIkVK
K219-u SGSCGPIkVKTEPTE
T238 SLEMAAVTVKEESED
K240 EMAAVTVKEESEDPD
Y248 EESEDPDYYQYNIQG
Y249 ESEDPDYYQYNIQGP
Y251 EDPDYYQYNIQGPSE
S318 TIEDDDYSPPTKRLK
S326-p PPTKRLKsTEPPPPP
K343 PEPANAGKRKVREFN
K353 VREFNFEKWNARITD
Y398 QSHVEDLYVEGLPEG
S412-p GIPFRRPsTYGIPRL
T413 IPFRRPsTYGIPRLE
S441-p KKHELLNsTREDLQL
K450 REDLQLDKPASGVKE
Y460 SGVKEEWYARITKLR
K476 MVDQLFCKKFAEALG
K477 VDQLFCKKFAEALGS
K488 ALGSTEAKAVPYQKF
K494 AKAVPYQKFEAHPND
Y503 EAHPNDLYVEGLPEN
S515-p PENIPFRsPSWYGIP
S517 NIPFRsPSWYGIPRL
Y519 PFRsPSWYGIPRLEK
K526 YGIPRLEKIIQVGNR
K539 NRIKFVIKRPELLTH
T556-p TEVTQPRtNtPVKED
T558-p VTQPRtNtPVKEDWN
K561 PRtNtPVKEDWNVRI
K561 PRtNtPVKEDWNVRI
S620 PEGIPFRSPTWFGIP
Y652 KPELVVSYLPPGMAS
S659 YLPPGMASKINTKAL
T663 GMASKINTKALQsPK
S668-p INTKALQsPKRPRsP
S674-p QsPKRPRsPGsNsKV
S677-p KRPRsPGsNsKVPEI
S679-p PRsPGsNsKVPEIEV
T687-p KVPEIEVtVEGPNNS
S695-p VEGPNNSsPQTSAVR
S699 NNSsPQTSAVRTPTQ
T703 PQTSAVRTPTQTNGS
T705 TSAVRTPTQTNGSNV
S722-p KPRGREFsFEAWNAK
K752 CGEALGLKQAVKVPF
Y770 ESFPEDFYVEGLPEG
S784-p GVPFRRPstFGIPRL
T785-p VPFRRPstFGIPRLE
S818 FETAIKESTssKsPP
S820-p TAIKESTssKsPPRK
S821-p AIKESTssKsPPRKI
S823-p KESTssKsPPRKINs
S830-p sPPRKINssPNVNTT
S831-p PPRKINssPNVNTTA
T836 NssPNVNTTAsGVED
T837 ssPNVNTTAsGVEDL
S839-p PNVNTTAsGVEDLNI
K879-u VNDLFSRkFGEAIGM
Y920-p VSFKAPSyLEISSMR
S925 PSyLEISSMRRILDS
K991 TDGSSQIKQEPDPTW
  GTF2I iso8  
K74 NTRKDFQKDFVKYCV
S103 TTQANRMSVDAVEIE
K140 VVPVPYEKMLRDQSA
K160 LPEGVAFKHPEHYDL
A202 LGGRVLAAEAERSML
S207 LAAEAERSMLSPSGS
S210 EAERSMLSPSGSCGP
S214 SMLSPSGSCGPIKVK
K219 SGSCGPIKVKTEPTE
T238 SLEMAAVTVKEESED
K240 EMAAVTVKEESEDPD
Y248 EESEDPDYYQYNIQG
Y249 ESEDPDYYQYNIQGS
Y251 EDPDYYQYNIQGSHH
S278 PAEDDDYSPPTKRLK
S286 PPTKRLKSTEPPPPP
K303 PEPANAGKRKVREFN
K313 VREFNFEKWNARITD
Y358 QSHVEDLYVEGLPEG
S372-p GIPFRRPsTYGIPRL
T373 IPFRRPsTYGIPRLE
S401 KKHELLNSTREDLQL
K410 REDLQLDKPASGVKE
Y420 SGVKEEWYARITKLR
K436 MVDQLFCKKFAEALG
K437 VDQLFCKKFAEALGS
K448 ALGSTEAKAVPYQKF
K454 AKAVPYQKFEAHPND
Y463 EAHPNDLYVEGLPEN
S475 PENIPFRSPSWYGIP
S477 NIPFRSPSWYGIPRL
Y479 PFRSPSWYGIPRLEK
K486 YGIPRLEKIIQVGNR
K499 NRIKFVIKRPELLTH
T516 TEVTQPRTNTPVKED
T518 VTQPRTNTPVKEDWN
K521 PRTNTPVKEDWNVRI
K521 PRTNTPVKEDWNVRI
S580 PEGIPFRSPTWFGIP
Y612 KPELVVSYLPPGMAS
S619 YLPPGMASKINTKAL
T623 GMASKINTKALQSPK
S628 INTKALQSPKRPRSP
S634 QSPKRPRSPGSNSKV
S637 KRPRSPGSNSKVPEI
S639 PRSPGSNSKVPEIEV
T647 KVPEIEVTVEGPNNS
S655 VEGPNNSSPQTSAVR
S659 NNSSPQTSAVRTPTQ
T663 PQTSAVRTPTQTNGS
T665 TSAVRTPTQTNGSNV
S682 KPRGREFSFEAWNAK
K712 CGEALGLKQAVKVPF
Y730 ESFPEDFYVEGLPEG
S744-p GVPFRRPsTFGIPRL
T745 VPFRRPsTFGIPRLE
S778 FETAIKESTSSKsPP
S780 TAIKESTSSKsPPIF
S781 AIKESTSSKsPPIFL
S783-p KESTSSKsPPIFLSG
S794 FLSGKINSSPNVNTT
S795 LSGKINSSPNVNTTA
T800 NSSPNVNTTASGVED
T801 SSPNVNTTASGVEDL
S803 PNVNTTASGVEDLNI
K843 VNDLFSRKFGEAIGM
Y884 VSFKAPSYLEISSMR
S889 PSYLEISSMRRILDS
K955 TDGSSQIKQEPDPTW
  rat

 
K74 NTRKDFQKDFVKYCV
S103 TTQANRMSVDAVEIE
K140 VVPVPYEKMLRDQSA
K160 LPEGVAFKHPEHYDL
A202 LGGRVMAADADRPML
P207 MAADADRPMLSPGGS
S210 DADRPMLSPGGSCGP
S214 PMLSPGGSCGPIKVK
K219 GGSCGPIKVKTEPTE
T238 SLEMAAVTVKEESED
K240 EMAAVTVKEESEDPD
Y248 EESEDPDYYQYNIQG
Y249 ESEDPDYYQYNIQGS
Y251 EDPDYYQYNIQGSHH
S299 TIEDDDYSPPTKRPK
S307 PPTKRPKSSEPPPPP
K324 PEPTNAGKRKVREFN
K334 VREFNFEKWNARITD
Y379 QSHVEDLYVEGLPEG
S393-p GIPFRRPsTYGIPRL
T394 IPFRRPsTYGIPRLE
S422 KKHELLNSTREDLQL
K431 REDLQLDKPASGVKE
Y441 SGVKEEWYARITKLR
K457 MVDQLFCKKFAEALG
K458 VDQLFCKKFAEALGS
K469 ALGSTEAKAVPYQKF
K475 AKAVPYQKFEAYPND
Y484 EAYPNDLYVEGLPEN
S496 PENIPFRSPSWYGIP
S498 NIPFRSPSWYGIPRL
Y500 PFRSPSWYGIPRLEK
K507 YGIPRLEKIIQVGNR
K520 NRIKFVIKRPELLTH
T537 TEVTQPRTNTPVKED
T539 VTQPRTNTPVKEDWN
K542 PRTNTPVKEDWNVRI
K542 PRTNTPVKEDWNVRI
S601 PEGIPFRSPTWFGIP
Y633 KPELVVSYLPPGMAS
S640 YLPPGMASKINTKAL
T644 GMASKINTKALQSPK
S649 INTKALQSPKRPRSP
S655 QSPKRPRSPGSNSKV
S658 KRPRSPGSNSKVPEI
S660 PRSPGSNSKVPEIEV
T668 KVPEIEVTVEGPNNN
S676 VEGPNNNSPQTSTVR
S680 NNNSPQTSTVRTPTQ
T684 PQTSTVRTPTQTNGS
T686 TSTVRTPTQTNGSNV
S703 KPRGREFSFEAWNAK
K733 CGEALGLKQAVKVPF
Y751 ESFPEDFYVEGLPEG
S765-p GVPFRRPsTFGIPRL
T766 VPFRRPsTFGIPRLE
S799 FETAIKESTSSKSPP
S801 TAIKESTSSKSPPRK
S802 AIKESTSSKSPPRKT
S804 KESTSSKSPPRKTNS
S811 SPPRKTNSSPSVNTT
S812 PPRKTNSSPSVNTTA
T817 NSSPSVNTTASGVED
T818 SSPSVNTTASGVEDL
S820 PSVNTTASGVEDLNI
K860 VNDLFSRKFGEAIGM
Y901 VSFKAPSYLEISSMR
S906 PSYLEISSMRRILDS
K972 TDGSSQIKQEPDPTW
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