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PU.1 (human)

Overview PU.1 Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing. Binds DNA as a monomer. Interacts with CEBPD and NONO. Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Highly expressed in both FV-P and FV-A-induced erythro- leukemia cell lines that have undergone rearrangements of the Spi- 1 gene due to the insertion of SFFV. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB. Protein type: DNA binding protein; Transcription factor Cellular Component: nuclear chromatin Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; NFAT protein binding; RNA binding; transcription factor activity Biological Process: macrophage differentiation; granulocyte differentiation; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; myeloid dendritic cell differentiation; lymphoid progenitor cell differentiation; negative regulation of transcription from RNA polymerase II promoter; anatomical structure regression; regulation of erythrocyte differentiation; vasculature development; lymphocyte differentiation; negative regulation of gene expression, epigenetic; induction of apoptosis; negative regulation of MHC class II biosynthetic process; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent Reference #:  P17947 (UniProtKB) Alt. Names/Synonyms: 31 kDa transforming protein; 31 kDa-transforming protein; hematopoietic transcription factor PU.1; OF; PU.1; SFPI1; SPI-1; SPI-1 proto-oncogene; SPI-A; SPI1; spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1 Gene Symbols: SPI1 Molecular weight: 31,083 Da Basal Isoelectric point: 6.31  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below PU.1 1PUE - E/F=171-259 (mouse)

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	Modification Sites and Domains

	Modification Sites in Parent Protein, Orthologs, and Isoforms

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		S17-p	FPLVPPPsEDLVPYD
1	0		S41	EYYPYLSSDGESHSD
1	0		S130	SLSPAQPSSDEEEGE
1	0		S131	LSPAQPSSDEEEGER
1	0		S140	EEEGERQSPPLEVSD
6	1		S146	QSPPLEVSDGEADGL

	mouse
S17	FSLTAPPSDDLVTYD
S41-p	DYYSFVGsDGESHSD
S132-p	LSPAHQQssDEEEGE
S133-p	SPAHQQssDEEEGER
S142-p	EEEGERQsPPLEVsD
S148-p	QsPPLEVsDGEADGL

	rat
S17	FPLVAPPSDELVTYE
G41	DYYSLIGGDGDSHSD
S131	LSPAHQQSSDEEEGE
S132	SPAHQQSSDEEEGER
S141	EEEGERQSPPLEVsD
S147-p	QSPPLEVsDGEADGL

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