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Protein Page:
PU.1 (human)

Overview
PU.1 Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing. Binds DNA as a monomer. Interacts with CEBPD and NONO. Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Highly expressed in both FV-P and FV-A-induced erythro- leukemia cell lines that have undergone rearrangements of the Spi- 1 gene due to the insertion of SFFV. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor
Cellular Component: nuclear chromatin
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; NFAT protein binding; RNA binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; macrophage differentiation; granulocyte differentiation; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; lymphoid progenitor cell differentiation; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; anatomical structure regression; regulation of transcription from RNA polymerase II promoter; regulation of erythrocyte differentiation; lymphocyte differentiation; negative regulation of gene expression, epigenetic; negative regulation of MHC class II biosynthetic process; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
Reference #:  P17947 (UniProtKB)
Alt. Names/Synonyms: 31 kDa transforming protein; 31 kDa-transforming protein; hematopoietic transcription factor PU.1; OF; PU.1; SFPI1; SPI-1; SPI-1 proto-oncogene; SPI-A; SPI1; spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1
Gene Symbols: SPI1
Molecular weight: 31,083 Da
Basal Isoelectric point: 6.31  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PU.1

Protein Structure Not Found.


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Modification Sites and Domains  

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 0 S41 EYYPYLSSDGESHSD
0 1 S125 CLQYPSLSPAQPSSD
1 1 S130 SLSPAQPSSDEEEGE
1 1 S131 LSPAQPSSDEEEGER
1 1 S140 EEEGERQSPPLEVSD
6 2 S146 QSPPLEVSDGEADGL
  mouse

 
S41-p DYYSFVGsDGESHSD
S126-p CFPYQTLsPAHQQss
S132-p LsPAHQQssDEEEGE
S133-p sPAHQQssDEEEGER
S142-p EEEGERQsPPLEVsD
S148-p QsPPLEVsDGEADGL
  rat

 
G41 DYYSLIGGDGDSHSD
S125 CFPYPPLSPAHQQSS
S131 LSPAHQQSSDEEEGE
S132 SPAHQQSSDEEEGER
S141 EEEGERQSPPLEVsD
S147-p QSPPLEVsDGEADGL
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