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Protein Page:
NFL (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NFL one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F). Note: This description may include information from UniProtKB.
Protein type: Cytoskeletal protein
Cellular Component: TSC1-TSC2 complex; axon; cytoplasm; neurofilament; cytosol
Molecular Function: protein C-terminus binding; protein binding, bridging; identical protein binding; protein binding; structural constituent of cytoskeleton
Biological Process: cell death; synaptic transmission; axon transport of mitochondrion; neurofilament bundle assembly; intermediate filament organization; anterograde axon cargo transport; retrograde axon cargo transport
Reference #:  P07196 (UniProtKB)
Alt. Names/Synonyms: 68 kDa neurofilament protein; CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; NEFL; Neurofilament light polypeptide; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament-light; NF-L; NF68; NFL
Gene Symbols: NEFL
Molecular weight: 61,517 Da
Basal Isoelectric point: 4.64  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NFL

Protein Structure Not Found.


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Sites Implicated In
molecular association, regulation: T21‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
2 1 S3-p _____MSsFSYEPYY
3 0 S13 YEPYYSTSykRRYVE
0 1 Y14-p EPYYSTSykRRYVEt
0 2 K15-ub PYYSTSykRRYVEtP
1 0 T21-p ykRRYVEtPRVHISS
1 0 T21 ykRRYVETPRVHISS
0 1 R23 RRYVEtPRVHISSVR
0 1 R23 RRYVEtPRVHISSVR
2 1 S27 EtPRVHISSVRSGYS
1 1 S27 EtPRVHISSVRSGYS
2 0 S28 tPRVHISSVRSGYST
0 1 R30 RVHISSVRSGYSTAR
2 0 S34 SSVRSGYSTARSAYS
1 0 S34 SSVRSGYSTARSAYS
1 2 S38 SGYSTARSAYSSYSA
0 47 Y40 YSTARSAYSSYSAPV
3 1 S42 TARSAYSSYSAPVSS
0 77 Y43 ARSAYSSYSAPVSSS
1 0 S44 RSAYSSYSAPVSSSL
1 2 S48 SSYSAPVSSSLSVRR
0 1 S49 SYSAPVSSSLSVRRs
2 0 S50 YSAPVSSSLSVRRsY
0 1 S50 YSAPVSSSLSVRRsY
2 1 S52 APVSSSLSVRRsYSS
0 1 S52 APVSSSLSVRRsYSS
6 2 S56-p SSLSVRRsYSSSSGS
4 0 S58 LSVRRsYSSSSGSLM
2 0 S63 sYSSSSGSLMPSLEN
2 1 S67 SSGSLMPSLENLDLS
1 0 N70 SLMPSLENLDLSQVA
0 1 S74 SLENLDLSQVAAISN
0 9 K91 KSIRTQEKAQLQDLN
0 4 S103-p DLNDRFAsFIERVHE
0 1 T154-p RLAAEDAtNEkQALQ
0 2 K157 AEDAtNEKQALQGER
0 1 K157-ub AEDAtNEkQALQGER
0 1 Y178 LRNLQARYEEEVLSR
0 2 S184 RYEEEVLSREDAEGR
0 2 S215-p ELEKRIDsLMDEIsF
0 1 S221-p DsLMDEIsFLKKVHE
0 2 K224 MDEIsFLKKVHEEEI
0 1 K225 DEIsFLKKVHEEEIA
0 2 K267 DIRAQYEKLAAkNMQ
0 3 K271-ub QYEKLAAkNMQNAEE
0 2 K281 QNAEEWFKSRFTVLT
0 2 K281 QNAEEWFKSRFTVLT
0 1 T288 KSRFTVLTESAAKNT
0 2 S290 RFTVLTESAAKNTDA
0 10 K293 VLTESAAKNTDAVRA
0 1 K315 SRRLLKAKTLEIEAC
0 1 K331 GMNEALEKQLQELED
0 1 K362-ac ENELRTTkSEMARYL
0 2 K370 SEMARYLKEyQDLLN
0 7 K370-ub SEMARYLkEyQDLLN
0 4 Y372-p MARYLkEyQDLLNVk
0 1 K379-ac yQDLLNVkMALDIEI
0 3 Y389-p LDIEIAAyRKLLEGE
0 8 K391 IEIAAyRKLLEGEET
0 1 Y412 VGSITSGYSQSSQVF
0 1 S413 GSITSGYSQSSQVFG
0 1 S422 SSQVFGRSAYGGLQT
0 66 Y424 QVFGRSAYGGLQTSS
0 1 S431 YGGLQTSSYLMSTRS
0 110 Y432 GGLQTSSYLMSTRSF
1 1 S435 QTSSYLMSTRSFPSY
0 1 S438 SYLMSTRSFPSYYTS
0 1 Y442 STRSFPSYYTSHVQE
0 1 Y443 TRSFPSYYTSHVQEE
0 1 I452 SHVQEEQIEVEETIE
1 11 S472-p EAKDEPPsEGEAEEE
0 1 S502 EEAAKEESEEAKEEE
0 5 G532 EEEKKVEGAGEEQAA
  mouse

 
S3-p _____MSsFGYDPYF
S13-p YDPYFSTsYkRRYVE
Y14 DPYFSTsYkRRYVET
K15-ub PYFSTsYkRRYVETP
T21 YkRRYVETPrVHIss
T21 YkRRYVETPrVHIss
R23-m2 RRYVETPrVHIssVr
R23-m1 RRYVETPrVHIssVr
S27-p ETPrVHIssVrSGYs
S27-gl ETPrVHIssVrSGYs
S28-p TPrVHIssVrSGYsT
R30-m1 rVHIssVrSGYsTAR
S34-p ssVrSGYsTARsAYS
S34 ssVrSGYSTARsAYS
S38-p SGYsTARsAYSsysA
Y40 YsTARsAYSsysAPV
S42-p TARsAYSsysAPVss
Y43-p ARsAYSsysAPVsss
S44-p RsAYSsysAPVsssL
S48-gl SsysAPVsssLsVRR
S49-gl sysAPVsssLsVRRs
S50-p ysAPVsssLsVRRsY
S50-gl ysAPVsssLsVRRsY
S52-p APVsssLsVRRsYsS
S52-gl APVsssLsVRRsYsS
S56-p ssLsVRRsYsSSSGs
S58-p LsVRRsYsSSSGsLM
S63-p sYsSSSGsLMPsLEN
S67-p SSGsLMPsLENLDLS
N70 sLMPsLENLDLSQVA
S74 sLENLDLSQVAAISN
K91-ub KSIRTQEkAQLQDLN
S103-p DLNDRFAsFIERVHE
T154 RLAAEDATNEKQALQ
K157 AEDATNEKQALQGER
K157 AEDATNEKQALQGER
Y178 LRNLQARYEEEVLsR
S184-p RYEEEVLsREDAEGR
S215-p ELEKRIDsLMDEIAF
A221 DsLMDEIAFLKKVHE
K224 MDEIAFLKKVHEEEI
K225 DEIAFLKKVHEEEIA
K268-ub DIRAQYEkLAAkNMQ
K272-ub QYEkLAAkNMQNAEE
K282 QNAEEWFKSRFTVLT
K282-ub QNAEEWFkSRFTVLT
T289 kSRFTVLTEsAAkNT
S291-p RFTVLTEsAAkNTDA
K294-ub VLTEsAAkNTDAVRA
K316-ub SRRLLKAkTLEIEAC
K332-ub GMNEALEkQLQELED
K363 ENELRSTKSEMARYL
K371 SEMARYLKEYQDLLN
K371-ub SEMARYLkEYQDLLN
Y373 MARYLkEYQDLLNVK
K380 YQDLLNVKMALDIEI
Y390 LDIEIAAYRkLLEGE
K392-ub IEIAAYRkLLEGEET
Y413 VGSITSGYsQSSQVF
S414-gl GSITSGYsQSSQVFG
S423-gl SSQVFGRsAySGLQS
Y425-p QVFGRsAySGLQSSS
S432 ySGLQSSSyLMsARs
Y433-p SGLQSSSyLMsARsF
S436-p QSSSyLMsARsFPAY
S439-p SyLMsARsFPAYYTS
Y443 sARsFPAYYTSHVQE
Y444 ARsFPAYYTSHVQEE
T453-p SHVQEEQtEVEETIE
S473-p EAKDEPPsEGEAEEE
S503-p EEAAKDEsEDTKEEE
S532-p EEEKKEEsAGEEQVA
  rat

 
S3-p _____MSsFSYEPYF
S13-p YEPYFSTsYKRRYVE
Y14 EPYFSTsYKRRYVEt
K15 PYFSTsYKRRYVEtP
T21 YKRRYVETPRVHIsS
T21-gl YKRRYVEtPRVHIsS
R23 RRYVEtPRVHIsSVR
R23 RRYVEtPRVHIsSVR
S27 EtPRVHISSVRSGYs
S27-gl EtPRVHIsSVRSGYs
S28 tPRVHIsSVRSGYsT
R30 RVHIsSVRSGYsTAR
S34 sSVRSGYSTARsAyS
S34-gl sSVRSGYsTARsAyS
S38-p SGYsTARsAySsySA
Y40-p YsTARsAySsySAPV
S42-p TARsAySsySAPVsS
Y43-p ARsAySsySAPVsSs
S44 RsAySsySAPVsSsL
S48-gl SsySAPVsSsLSVRR
S49 sySAPVsSsLSVRRs
S50-p ySAPVsSsLSVRRsY
S50 ySAPVsSSLSVRRsY
S52 APVsSsLSVRRsYsS
S52 APVsSsLSVRRsYsS
S56-p SsLSVRRsYsSSSGS
S58-p LSVRRsYsSSSGSLM
S63 sYsSSSGSLMPsLEN
S67-p SSGSLMPsLENLDLs
N70 SLMPsLENLDLsQVA
S74-p sLENLDLsQVAAISN
K91-ub KSIRTQEkAQLQDLN
S103-p DLNDRFAsFIERVHE
T154 RLAAEDATNEkQALQ
K157-ac AEDATNEkQALQGER
K157 AEDATNEKQALQGER
Y178-p LRNLQARyEEEVLsR
S184-p RyEEEVLsREDAEGR
S215 ELEKRIDSLMDEIAF
A221 DSLMDEIAFLkkVHE
K224-ac MDEIAFLkkVHEEEI
K225-ac DEIAFLkkVHEEEIA
K268 DIRAQYEKLAAKNMQ
K272 QYEKLAAKNMQNAEE
K282-ac QNAEEWFkSRFTVLt
K282 QNAEEWFKSRFTVLt
T289-p kSRFTVLtEsAAKNT
S291-p RFTVLtEsAAKNTDA
K294 VLtEsAAKNTDAVRA
K316 SRRLLKAKTLEIEAC
K332 GMNEALEKQLQELED
K363-ac ENELRSTkSEMARYL
K371-ac SEMARYLkEyQDLLN
K371 SEMARYLKEyQDLLN
Y373-p MARYLkEyQDLLNVK
K380 yQDLLNVKMALDIEI
Y390-p LDIEIAAyRKLLEGE
K392 IEIAAyRKLLEGEET
Y413-p VGSITSGySQSSQVF
S414 GSITSGySQSSQVFG
S423 SSQVFGRSAySGLQS
Y425-p QVFGRSAySGLQSSs
S432-p ySGLQSSsyLMsARA
Y433-p SGLQSSsyLMsARAF
S436-p QSSsyLMsARAFPAy
A439 syLMsARAFPAyyTS
Y443-p sARAFPAyyTSHVQE
Y444-p ARAFPAyyTSHVQEE
S453-p SHVQEEQsEVEETIE
S473-p EAKDEPPsEGEAEEE
S503-p EEAAKDEsEDAKEEE
S531-p EEEKKEEsAGEEQAA
  pig

 
S3 _____MSSFYSEPYY
S13-p SEPYYSTsYKRRYVE
Y14 EPYYSTsYKRRYVET
K15 PYYSTsYKRRYVETP
T21 YKRRYVETPRVHISs
T21 YKRRYVETPRVHISs
R23 RRYVETPRVHISsVR
R23 RRYVETPRVHISsVR
S27 ETPRVHISsVRSGYs
S27 ETPRVHISsVRSGYs
S28-p TPRVHISsVRSGYsT
R30 RVHISsVRSGYsTAR
S34-p SsVRSGYsTARSAYS
S34 SsVRSGYSTARSAYS
S38 SGYsTARSAYSSYSA
Y40 YsTARSAYSSYSAPV
S42 TARSAYSSYSAPVSS
Y43 ARSAYSSYSAPVSSS
S44 RSAYSSYSAPVSSSL
S48 SSYSAPVSSSLsVRR
S49 SYSAPVSSSLsVRRS
S50 YSAPVSSSLsVRRSY
S50 YSAPVSSSLsVRRSY
S52-p APVSSSLsVRRSYSS
S52 APVSSSLSVRRSYSS
S56 SSLsVRRSYSSSSGL
S58 LsVRRSYSSSSGLMP
- gap
S66 SSSGLMPSLENLDLS
N69 GLMPSLENLDLSQVA
S73 SLENLDLSQVAAISN
K90 KSIRTQEKAQLQDLN
S102 DLNDRFASFIERVHE
T153 RLAAEDATNEKQALQ
K156 AEDATNEKQALQGER
K156 AEDATNEKQALQGER
Y177 LRNLQARYEEEVLSR
S183 RYEEEVLSREDAEGR
S214 ELEKRIDSLMDEIAF
A220 DSLMDEIAFLKKVHE
K223 MDEIAFLKKVHEEEI
K224 DEIAFLKKVHEEEIA
K267 DIRAQYEKLAAKNMQ
K271 QYEKLAAKNMQNAEE
K281 QNAEEWFKSRFTVLT
K281 QNAEEWFKSRFTVLT
T288 KSRFTVLTESAAKNT
S290 RFTVLTESAAKNTDA
K293 VLTESAAKNTDAVRA
K315 SRRLLKAKTLEIEAC
K331 GMNEALEKQLQELED
K362 ENELRTTKSEMARYL
K370 SEMARYLKEYQDLLN
K370 SEMARYLKEYQDLLN
Y372 MARYLKEYQDLLNVK
K379 YQDLLNVKMALDIEI
Y389 LDIEIAAYRKLLEGE
K391 IEIAAYRKLLEGEET
Y412 VGSLTTGYSQSSQVF
S413 GSLTTGYSQSSQVFG
S422 SSQVFGRSAYGGLQT
Y424 QVFGRSAYGGLQTSS
S431 YGGLQTSSYLMSTRS
Y432 GGLQTSSYLMSTRSF
S435 QTSSYLMSTRSFPSY
S438 SYLMSTRSFPSYYTS
Y442 STRSFPSYYTSHVQE
Y443 TRSFPSYYTSHVQEE
I452 SHVQEEQIEVEETIE
S472 EAKDEPPSEGEAEEE
S509 EAAEKEESEEAKEEE
G538 EEEKKDEGAGEEQAT
  cow

 
S3 _____MSSFSYEPYY
S13 YEPYYSTSYKRRYVE
Y14 EPYYSTSYKRRYVET
K15 PYYSTSYKRRYVETP
T21 YKRRYVETPRVHIsS
T21 YKRRYVETPRVHIsS
R23 RRYVETPRVHIsSVR
R23 RRYVETPRVHIsSVR
S27-p ETPRVHIsSVRSGYS
S27 ETPRVHISSVRSGYS
S28 TPRVHIsSVRSGYST
R30 RVHIsSVRSGYSTAR
S34 sSVRSGYSTARSAYS
S34 sSVRSGYSTARSAYS
S38 SGYSTARSAYSsYSA
Y40 YSTARSAYSsYSAPV
S42-p TARSAYSsYSAPVSS
Y43 ARSAYSsYSAPVSSS
S44 RSAYSsYSAPVSSSL
S48 SsYSAPVSSSLSVRR
S49 sYSAPVSSSLSVRRs
S50 YSAPVSSSLSVRRsY
S50 YSAPVSSSLSVRRsY
S52 APVSSSLSVRRsYsS
S52 APVSSSLSVRRsYsS
S56-p SSLSVRRsYsSSSGs
S58-p LSVRRsYsSSSGsLM
S63-p sYsSSSGsLMPsLEs
S67-p SSGsLMPsLEsLDLS
S70-p sLMPsLEsLDLSQVA
S74 sLEsLDLSQVAAISN
K91 KSIRTQEKAQLQDLN
S103 DLNDRFASFIERVHE
T154 RLAAEDATNEKQALQ
K157 AEDATNEKQALQGER
K157 AEDATNEKQALQGER
Y178 LRNLQARYEEEVLSR
S184 RYEEEVLSREDAEGR
S215 ELEKRIDSLMDEIAF
A221 DSLMDEIAFLKKVHE
K224 MDEIAFLKKVHEEEI
K225 DEIAFLKKVHEEEIA
K268 DIRAQYEKLAAKNMQ
K272 QYEKLAAKNMQNAEE
K282 QNAEEWFKSRFTVLT
K282 QNAEEWFKSRFTVLT
T289 KSRFTVLTESAAKNT
S291 RFTVLTESAAKNTDA
K294 VLTESAAKNTDAVRA
K316 SRRLLKAKTLEIEAC
K332 GMNEALEKQLQELED
K363 ENELRTTKSEMARYL
K371 SEMARYLKEYQDLLN
K371 SEMARYLKEYQDLLN
Y373 MARYLKEYQDLLNVK
K380 YQDLLNVKMALDIEI
Y390 LDIEIAAYRKLLEGE
K392 IEIAAYRKLLEGEET
Y413 VGSLTTGYTQSSQVF
T414 GSLTTGYTQSSQVFG
S423 SSQVFGRSAYGGLQT
Y425 QVFGRSAYGGLQTSS
S432 YGGLQTSSYLMSARS
Y433 GGLQTSSYLMSARSF
S436 QTSSYLMSARSFPSY
S439 SYLMSARSFPSYYTS
Y443 SARSFPSYYTSHVQE
Y444 ARSFPSYYTSHVQEE
I453 SHVQEEQIEVEETIE
S473-p EAKDEPPsEGEAEEE
A515 EEAAKEDAEEAKEEE
G544 EEEKKDEGAGEEQAT
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