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Chk2 (human)

Overview Chk2 a CAMK protein kinase of the Rad53 family. A cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. Phosphorylates and inhibits CDC25C, preventing entry into mitosis, p53, leading to cell cycle arrest in G1, and BRCA1, restoring survival after DNA damage. Twelve splice variant isoforms have been described for human Chk2. LOF mutants cause Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype also caused by p53 mutations. Familial mutations also associated with prostate and breast cancer, and mutations also seen in a variety of sporadic cancers and cell lines. Note: This description may include information from UniProtKB. Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, CAMK; CAMK group; RAD53 family Cellular Component: nucleoplasm; PML body; chromosome, telomeric region Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; metal ion binding; ubiquitin protein ligase binding; protein kinase binding; ATP binding Biological Process: DNA damage induced protein phosphorylation; transcription, DNA-dependent; protein amino acid autophosphorylation; positive regulation of transcription, DNA-dependent; DNA damage response, signal transduction; regulation of transcription, DNA-dependent; DNA damage response, signal transduction resulting in induction of apoptosis; cellular protein catabolic process; cell division; double-strand break repair; DNA damage checkpoint; response to gamma radiation; regulation of protein catabolic process; G2/M transition of mitotic cell cycle; response to DNA damage stimulus; cell cycle checkpoint Reference #:  O96017 (UniProtKB) Alt. Names/Synonyms: Cds1; checkpoint-like protein CHK2; CHEK2; CHK2; CHK2 checkpoint homolog (S. pombe); HuCds1; LFS2; PP1425; protein kinase CHK2; RAD53; Serine/threonine-protein kinase Chk2 Gene Symbols: CHEK2 Molecular weight: 60,915 Da Basal Isoelectric point: 5.65  Predict pI for various phosphorylation states CST Pathways:  Apoptosis  |  Cell Cycle: G1/S Checkpoint  |  Cell Cycle: G2/M DNA Damage Checkpoint Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below Chk2 1GXC - A/D/G/J=64-212 (human) 2CN5 - A=210-531 (human) 2CN8 - A=210-531 (human) 2W0J - A=210-531 (human) 2W7X - A=210-531 (human) 2WTC - A=210-531 (human) 2WTD - A=210-531 (human) 2XBJ - A=210-531 (human) 2XM8 - A=210-531 (human) 2XM9 - A=210-531 (human) 2YCF - A=210-530 (human) 2YCQ - A=210-531 (human) 2YCR - A=210-531 (human) 2YCS - A=210-531 (human) 2YIQ - A=210-531 (human) 2YIR - A=210-531 (human) 2YIT - A=210-531 (human) 3I6U - A/B=84-502 (human) 3I6W - A/B/C/D/E/F/G/H=70-512 (human) 3VA4 - C=63-73 (human) 4A9R - A=210-531 (human) 4A9S - A=210-531 (human) 4A9T - A=210-531 (human) 4A9U - A=210-531 (human) Substrate Sequence Logo

STRING  |  Scansite  |  KinBase  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1GXC 2CN5 2CN8 2W0J 2W7X 2WTC 2WTD 2XBJ 2XM8 2XM9 2YCF 2YCQ 2YCR 2YCS 2YIQ 2YIR 2YIT 3I6U 3I6W 3VA4 4A9R 4A9S 4A9T 4A9U  |  ENZYME  |  Phospho3D  |  DISEASE 151623 604373  |  Source  |  InnateDB  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene

	Sites Implicated In
apoptosis, induced: T68, S379, S456 cell cycle regulation: S19, S33, S35, T68, S73, S164, T205, S210, T387 cytoskeletal reorganization: T68 enzymatic activity, induced: S19, T26, S28, S33, S35, T68, S73, S140, S379, T383, T387, Y390, S516 enzymatic activity, inhibited: S164, T205, S210 intracellular localization: T68, S372, T378, S379, T383, T387, T389, Y390 molecular association, regulation: S19, S33, S35, T68, S73 phosphorylation: T26, S28, T68, S73, T389 protein conformation: T68, S140, T383, T387 protein degradation: S456 ubiquitination: S379, T383, S456

	Modification Sites and Domains
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	Modification Sites in Parent Protein, Orthologs, and Isoforms

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human ► Hide Isoforms
0	1		S12	SDVEAQQsHGsSACs
0	1		S15	EAQQsHGsSACsQPH
11	1		S19	sHGsSACsQPHGsVt
0	1		S24	ACsQPHGsVtQsQGS
3	1		T26	sQPHGsVtQsQGSSs
3	0		S28	PHGsVtQsQGSSsQs
10	1		S33	tQsQGSSsQsQGIss
9	1		S35	sQGSSsQsQGIssss
0	1		S39	SsQsQGIsssststM
0	1		S40	sQsQGIsssststMP
0	1		S41	QsQGIsssststMPN
0	1		S42	sQGIsssststMPNS
0	1		T43	QGIsssststMPNSs
0	1		S44	GIsssststMPNSsQ
0	1		T45	IsssststMPNSsQs
0	1		S50	tstMPNSsQsSHsSS
0	1		S52	tMPNSsQsSHsSSGT
0	1		S55	NSsQsSHsSSGTLSs
1	0		S62	sSSGTLSsLEtVstQ
0	1		T65	GTLSsLEtVstQELY
0	1		S67	LSsLEtVstQELYsI
123	2		T68	SsLEtVstQELYsIP
1	1		S73	VstQELYsIPEDQEP
3	1		S120	YWFGRDKsCEYCFDE
1	0		S140	TDKYRTYsKKHFRIF
1	1		S164	IAYIEDHsGNGTFVN
1	1		T205	VFVFFDLtVDDQsVY
1	1		S210	DLtVDDQsVYPKALR
1	1		T225	DEYIMSKtLGSGACG
2	2		S260	KRKFAIGsAREADPA
1	1		S372	KITDFGHsKILGEts
2	2		T378	HsKILGEtsLMRtLC
5	5		S379	sKILGEtsLMRtLCG
7	3		T383	GEtsLMRtLCGtPty
16	2		T387	LMRtLCGtPtyLAPE
2	1		T389	RtLCGtPtyLAPEVL
1	1		Y390	tLCGtPtyLAPEVLV
4	2		T432	PPFSEHRtQVsLKDQ
1	1		S435	SEHRtQVsLKDQITS
1	0		S456	PEVWAEVsEKALDLV
11	3		S516	PQVLAQPstSRKRPR
1	0		T517	QVLAQPstSRKRPRE
0	1		A537	AETTKRPAVCAAVL_

	Chk2 iso12
S12	SDVEAQQSHGSSACS
S15	EAQQSHGSSACSQPH
S19	SHGSSACSQPHGSVT
S24	ACSQPHGSVTQSQGS
T26	SQPHGSVTQSQGSSS
S28	PHGSVTQSQGSSSQS
S33	TQSQGSSSQSQGISS
S35	SQGSSSQSQGISSSS
S39	SSQSQGISSSSTSTM
S40	SQSQGISSSSTSTMP
S41	QSQGISSSSTSTMPN
S42	SQGISSSSTSTMPNS
T43	QGISSSSTSTMPNSS
S44	GISSSSTSTMPNSSQ
T45	ISSSSTSTMPNSSQS
S50	TSTMPNSSQSSHSSS
S52	TMPNSSQSSHSSSGT
S55	NSSQSSHSSSGTLSS
S62	SSSGTLSSLETVSTQ
T65	GTLSSLETVSTQELY
S67	LSSLETVSTQELYSI
T68	SSLETVSTQELYSIP
S73	VSTQELYSIPEDQEP
S120	YWFGRDKSCEYCFDE
S140	TDKYRTYSKKHFRIF
S164	IAYIEDHSGNGTFVN
T205	VFVFFDLTVDDQSVY
S210	DLTVDDQSVYPKALR
T225	DEYIMSKTLGSGACG
S260	KRKFAIGSAREADPA
S343	QITDFGHSKILGETS
T349	HSKILGETSLMRTLC
S350	SKILGETSLMRTLCG
T354	GETSLMRTLCGTPTY
T358	LMRTLCGTPTYLAPE
T360	RTLCGTPTYLAPEVL
Y361	TLCGTPTYLAPEVLV
T403	PPFSEHRTQVSLKDQ
S406	SEHRTQVSLKDQITS
S427	PEVWAEVSEKALDLV
S487	PQVLAQPSTSRKRPR
T488	QVLAQPSTSRKRPRE
A508	AETTKRPAVCAAVL_

	mouse
A15	HSSTSSKAHDSASCS
S18	TSSKAHDSASCSQSQ
S22	AHDSASCSQSQGGFS
G27	SCSQSQGGFSQPQGT
S29	SQSQGGFSQPQGTPS
P31	SQGGFSQPQGTPSQL
S36	SQPQGTPSQLHELSQ
S42	PSQLHELSQYQGSSS
S48	LSQYQGSSSSSTGTV
S49	SQYQGSSSSSTGTVP
S50	QYQGSSSSSTGTVPS
S51	YQGSSSSSTGTVPSS
T52	QGSSSSSTGTVPSSS
G53	GSSSSSTGTVPSSSQ
T54	SSSSSTGTVPSSSQS
S59	TGTVPSSSQSSHSSS
S61	TVPSSSQSSHSSSGT
S64	SSSQSSHSSSGTLSS
S71	SSSGTLSSLETVStQ
T74	GTLSSLETVStQELC
S76	LSSLETVStQELCSI
T77	SSLETVStQELCSIP
S82	VStQELCSIPEDQEP
S124	YWFGRDKSCEYCFDG
S144	TDKYRTYSKKHFRIF
S168	IVYIEDHSGNGTFVN
T209	VFVFFDLTVDDQSVY
S214	DLTVDDQSVYPKELR
T229	DEYIMSKTLGSGACG
S264	KRRFALGSSREADTA
S376	KITDFGQSKILGETs
T382	QSKILGETsLMRTLC
S383	SKILGETsLMRTLCG
T387	GETsLMRTLCGTPTY
T391	LMRTLCGTPTYLAPE
T393	RTLCGTPTYLAPEVL
Y394	TLCGTPTYLAPEVLV
T436	PPFSEHKTQVSLKDQ
S439	SEHKTQVSLKDQITS
S460	PEVWTDVSEEALDLV
S520	PVAPAQTSSQKRPLE
-	gap
S540	MPSTKRLsVCGAVL_

	rat
A14	HGSTSSKAHDSASCS
S17	TSSKAHDSASCSQSQ
S21	AHDSASCSQSQGGFS
G26	SCSQSQGGFSQSQGT
S28	SQSQGGFSQSQGTPS
S30	SQGGFSQSQGTPSQL
S35	SQSQGTPSQLHDLSQ
S41	PSQLHDLSQYQGASS
S47	LSQYQGASSSSTSTV
S48	SQYQGASSSSTSTVP
S49	QYQGASSSSTSTVPS
S50	YQGASSSSTSTVPSS
T51	QGASSSSTSTVPSSS
S52	GASSSSTSTVPSSSQ
T53	ASSSSTSTVPSSSQS
S58	TSTVPSSSQSSHSSS
S60	TVPSSSQSSHSSSGT
S63	SSSQSSHSSSGTLSS
S70	SSSGTLSSLETVSTQ
T73	GTLSSLETVSTQELC
S75	LSSLETVSTQELCSI
T76	SSLETVSTQELCSIP
S81	VSTQELCSIPEDQEP
S123	YWFGRDKSCEYCFDG
S143	TDKYRTYSKKHFRIF
S167	IVYLEDHSGNGTFVN
T208	VFVFFDLTVDDQSVY
S213	DLTVDDQSVYPKELR
T228	DEYIMSKTLGSGACG
S263	KRRFALGSSREADTA
S375	KITDFGQSKILGETS
T381	QSKILGETSLMRTLC
S382	SKILGETSLMRTLCG
T386	GETSLMRTLCGTPTY
T390	LMRTLCGTPTYLAPE
T392	RTLCGTPTYLAPEVL
Y393	TLCGTPTYLAPEVLI
T435	PPFSEHKTQVSLKDQ
S438	SEHKTQVSLKDQITS
S459	PEVWTDVSEKALDLV
S519	PLAPAQTSGQKRPLE
-	gap
A539	AESSKRLAVCKAVL_

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