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Protein Page:
Hic-5 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Hic-5 Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA- binding activity. Homooligomer. Interacts with CRIP2, HSPB1, ILK, LIMS1, LIMS2, NCK2, NUDT16L1, PAK, PPARG, PTPN12, TCF3, TCF7L2 and VCL. Forms a complex with GIT1 and ARHGEF7. Interacts with AR/androgen receptor in a ligand-dependent manner. Interacts with CSK, LYN, MAPK15, NR3C1, PPARG, PTK2/FAK1, PTK2B/PYK2, SLC6A3, SLC6A4, SMAD3, SRC and talin. Up-regulated by TNF and hydrogen peroxide. Expressed in platelets, smooth muscle and prostate stromal cells. Belongs to the paxillin family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; Transcription, coactivator/corepressor; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: extracellular matrix; focal adhesion; cytoskeleton; nuclear matrix; cytoplasm; intracellular
Molecular Function: protein binding; Roundabout binding; androgen receptor binding; zinc ion binding; transcription coactivator activity
Biological Process: transcription from RNA polymerase II promoter; Wnt receptor signaling pathway; cell fate commitment; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; negative regulation of fat cell differentiation; ubiquitin-dependent SMAD protein catabolic process; negative regulation of cell proliferation; morphogenesis of embryonic epithelium; epithelial cell differentiation; response to heat; androgen receptor signaling pathway; cell adhesion; negative regulation of transforming growth factor beta receptor signaling pathway
Reference #:  O43294 (UniProtKB)
Alt. Names/Synonyms: Androgen receptor coactivator 55 kDa protein; androgen receptor coactivator ARA55; Androgen receptor-associated protein of 55 kDa; ARA55; Hic-5; HIC5; Hydrogen peroxide-inducible clone 5 protein; TGFB1I1; TGFI1; transforming growth factor beta 1 induced transcript 1; Transforming growth factor beta-1-induced transcript 1 protein; TSC-5
Gene Symbols: TGFB1I1
Molecular weight: 49,814 Da
Basal Isoelectric point: 6.62  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Hic-5

Protein Structure Not Found.


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Sites Implicated In
transcription, altered: Y60‑p
activity, inhibited: Y60‑p
molecular association, regulation: Y60‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 16 T33-p ERPAEPLtPPPsyGH
0 1 S37-p EPLtPPPsyGHQPQT
1 2 Y38-p PLtPPPsyGHQPQTG
0 3 T44 syGHQPQTGSGESSG
0 3 A52 GSGESSGASGDKDHL
3 22 Y60-p SGDKDHLystVCKPR
0 6 S61-p GDKDHLystVCKPRs
0 1 T62-p DKDHLystVCKPRsP
0 10 S68-p stVCKPRsPKPAAPA
0 1 S122-p FPSSKVAsGEQKEDQ
0 1 S130-p GEQKEDQsEDKKRPs
0 27 S137-p sEDKKRPsLPssPsP
0 13 S140-p KKRPsLPssPsPGLP
1 27 S141-p KRPsLPssPsPGLPK
0 17 S143-p PsLPssPsPGLPKAS
0 3 S164-p ELDRLMAsLSDFRVQ
0 5 S186-p PTQPPVVsstNEGsP
0 1 S187-p TQPPVVsstNEGsPs
0 5 T188-p QPPVVsstNEGsPsP
0 5 S192-p VsstNEGsPsPPEPt
0 12 S194-p stNEGsPsPPEPtGK
0 2 T199-p sPsPPEPtGKGSLDT
0 1 G200 PsPPEPtGKGSLDTM
0 7 S403-p HFHARRGsLCAtCGL
0 2 T407-p RRGsLCAtCGLPVTG
0 2 S418-p PVTGRCVsALGRRFH
0 1 K458-ub YCQPCFLkLFG____
  mouse

 
T33-p ERPPETLtPPPPyGH
P37 ETLtPPPPyGHQPQt
Y38-p TLtPPPPyGHQPQtG
T44-p PyGHQPQtGSGESSG
T52-p GSGESSGtTGDKDHL
Y60-p TGDKDHLysTVCKPR
S61-p GDKDHLysTVCKPRs
T62 DKDHLysTVCKPRsP
S68-p sTVCKPRsPKPVAPV
E122 FPSSKMAEGEEKEDQ
S130 GEEKEDQSEDKSSPT
T137 SEDKSSPTVPPsPFP
P140 KSSPTVPPsPFPAPS
S141-p SSPTVPPsPFPAPSK
F143 PTVPPsPFPAPSKPS
S164-p ELDRLMAsLSDFRVQ
S186-p PPQPPAAsPtREGCP
P187 PQPPAAsPtREGCPs
T188-p QPPAAsPtREGCPsP
C192 AsPtREGCPsPPGQt
S194-p PtREGCPsPPGQtsK
T199-p CPsPPGQtsKGSLDT
S200-p PsPPGQtsKGSLDTM
S403-p HFHAQRGsLCAtCGL
T407-p QRGsLCAtCGLPVTG
S418-p PVTGRCVsALGRRFH
K458 YCQPCFLKLFG____
  rat

 
T33-p ERPPETLtPPPPYGH
P37 ETLtPPPPYGHQPQT
Y38 TLtPPPPYGHQPQTG
T44 PYGHQPQTGSGESSG
A52 GSGESSGASGDKDHL
Y60 SGDKDHLYSTVCKPR
S61 GDKDHLYSTVCKPRS
T62 DKDHLYSTVCKPRSP
S68 STVCKPRSPKSVAPV
E122 FPSSKMAEGEGKEDQ
S130 GEGKEDQSEDKSITT
T137 SEDKSITTVPSSTFP
S140 KSITTVPSSTFPAPS
S141 SITTVPSSTFPAPSK
T142 ITTVPSSTFPAPSKP
S164 ELDRLMASLSDFRVQ
S186 PPQPPAVSPTREGCP
P187 PQPPAVSPTREGCPs
T188 QPPAVSPTREGCPsP
C192 VSPTREGCPsPPGQT
S194-p PTREGCPsPPGQTNK
T199 CPsPPGQTNKGSLDT
N200 PsPPGQTNKGSLDTM
S403 HFHAQRGSLCATCGL
T407 QRGSLCATCGLPVTG
S418 PVTGRCVSALGRRFH
K458 YCQPCFLKLFG____
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