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Protein Page:
BLM (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
BLM a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2. Note: This description may include information from UniProtKB.
Protein type: DNA repair; Helicase; EC 3.6.4.12; EC 3.6.1.-; DNA replication
Cellular Component: male germ cell nucleus; lateral element; PML body; chromosome, telomeric region; nuclear matrix; pronucleus; cytoplasm; nucleolus; replication fork; nucleus
Molecular Function: G-quadruplex DNA binding; ATP-dependent DNA helicase activity; four-way junction helicase activity; protein binding; ATP-dependent 3'-5' DNA helicase activity; p53 binding; ATPase activity; ATP-dependent helicase activity; bubble DNA binding; helicase activity; single-stranded DNA binding; ATP binding
Biological Process: ATP catabolic process; negative regulation of DNA recombination; regulation of binding; positive regulation of transcription, DNA-dependent; DNA double-strand break processing; negative regulation of mitotic recombination; alpha-beta T cell differentiation; negative regulation of cell division; DNA repair; G2 phase of mitotic cell cycle; protein oligomerization; double-strand break repair via homologous recombination; DNA recombination; replication fork processing; replication fork protection; positive regulation of alpha-beta T cell proliferation; regulation of cyclin-dependent protein kinase activity; G2/M transition DNA damage checkpoint; telomere maintenance; response to DNA damage stimulus; response to X-ray
Reference #:  P54132 (UniProtKB)
Alt. Names/Synonyms: BLM; Bloom syndrome protein; Bloom syndrome, RecQ helicase-like; BS; DNA helicase, RecQ-like type 2; MGC126616; MGC131618; MGC131620; RecQ protein-like 3; RECQ2; RECQL2; RECQL3
Gene Symbols: BLM
Molecular weight: 159,000 Da
Basal Isoelectric point: 7.33  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

BLM
Protein Structure Not Found.


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Sites Implicated In
apoptosis, inhibited: T99‑p, T122‑p
cell cycle regulation: T99‑p, T122‑p, S144‑p
intracellular localization: S646‑p
molecular association, regulation: T99‑p, S646‑p
protein conformation: T122‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

 
0 1 T20-p LERHSARtLNNKLSL
0 1 S28-p LNNKLSLsKPKFSGF
0 1 T36-p KPKFSGFtFKKKTSS
0 3 S48-p TSSDNNVsVtNVsVA
0 1 T50-p SDNNVsVtNVsVAKt
0 2 S53-p NVsVtNVsVAKtPVL
0 5 T57-p tNVsVAKtPVLRNKD
0 4 T68-p RNKDVNVtEDFsFsE
0 9 S72-p VNVtEDFsFsEPLPN
0 1 S74-p VtEDFsFsEPLPNTT
0 2 - gap
4 0 T99-p NAPAGQEtQRGGSKS
0 1 F111 SKSLLPDFLQtPKEV
0 2 T114-p LLPDFLQtPKEVVCT
0 1 V118 FLQtPKEVVCTtQNt
3 0 T122-p PKEVVCTtQNtPTVK
0 4 T125-p VVCTtQNtPTVKKSR
0 1 T127 CTtQNtPTVKKSRDT
0 1 S142 ALKKLEFSssPDSLS
0 1 S143-p LKKLEFSssPDSLST
1 6 S144-p KKLEFSssPDSLSTI
0 1 S168-p DTSETSKsFVtPPQs
0 9 T171-p ETSKsFVtPPQsHFV
0 1 S175-p sFVtPPQsHFVRVST
0 1 S181 QsHFVRVSTAQKSKK
0 6 Y199-p NFFKAQLyTtNTVKT
0 1 T201-p FKAQLyTtNTVKTDL
0 1 P242 VICIDDGPIAEVHIN
0 1 A244 CIDDGPIAEVHINED
0 1 S269-p LEDERDNsEKKKNLE
0 1 Y296-p IEFDDDDyDTDFVPP
1 3 S304-p DTDFVPPsPEEIISA
1 0 K317-s SASSSSSkCLSTLKD
0 1 S328-p TLKDLDTsDRkEDVL
1 0 K331-s DLDTsDRkEDVLSTs
0 1 S336 DRkEDVLSTsKDLLS
0 2 S338-p kEDVLSTsKDLLSkP
1 0 K344-s TsKDLLSkPEkMSMQ
1 0 K347-s DLLSkPEkMSMQELN
0 2 S358-p QELNPETsTDCDARQ
0 1 T412-p NIRRKLLtEVDFNKs
0 3 S419-p tEVDFNKsDAsLLGs
0 9 S422-p DFNKsDAsLLGsLWR
0 1 S426-p sDAsLLGsLWRYRPD
0 9 S434-p LWRYRPDsLDGPMEG
0 2 F455 NSMKELNFSHLPSNS
0 1 S460 LNFSHLPSNSVsPGD
0 1 S462 FSHLPSNSVsPGDCL
0 1 S464-p HLPSNSVsPGDCLLT
0 1 T472 PGDCLLTTTLGKTGF
0 6 S499-p FNTHLQKsFVSSNWA
0 10 T508-p VSSNWAEtPRLGKKN
0 3 S539-p DQNKHTAsINDLERE
0 1 S550-p LERETQPsyDIDNFD
0 2 Y551-p ERETQPsyDIDNFDI
0 1 S601-p KSVSERLsSAKTDCL
1 0 S646-p LKHERFQsLSFPHTK
0 1 K657-a PHTKEMMkIFHkkFG
0 1 K661-a EMMkIFHkkFGLHNF
0 1 K662-a MMkIFHkkFGLHNFR
0 1 Y699-p GGGKSLCyQLPACVS
1 0 S714-p PGVTVVIsPLRSLIV
0 1 Y736-p SLDIPATyLTGDKTD
1 1 T766-p IIKLLYVtPEKICAs
0 1 S773-p tPEKICAsNRLISTL
0 1 T830-p SVPVMALtAtANPRV
0 1 T832-p PVMALtAtANPRVQK
0 1 K863-a SFNRHNLkYYVLPKK
0 1 S1128 SKSAKIQSGIFGkGS
0 1 K1133-u IQSGIFGkGSAYSRH
0 1 K1216 SQREEMVKKCLGELT
0 1 K1217 QREEMVKKCLGELTE
1 1 S1290-p QKYSEWTsPAEDssP
0 2 S1295-p WTsPAEDssPGISLS
1 5 S1296-p TsPAEDssPGISLSS
0 3 S1310-p SSRGPGRsAAEELDE
0 1 A1312 RGPGRsAAEELDEEI
0 1 K1411-a PINRPFLkPSYAFS_
0 1 S1417 LkPSYAFS_______
  mouse

 
K20 LQRHSARKLNNQPSL
S28 LNNQPSLSKPKSLGF
T36 KPKSLGFTFKKKTSE
S47 KTSEGDVSVTSVSVV
T49 SEGDVSVTSVSVVKT
S52 DVSVTSVSVVKTPAL
T56 TSVSVVKTPALSDKD
S67 SDKDVNVSEAFsFTE
S71-p VNVSEAFsFTEsPLH
T73 VSEAFsFTEsPLHKP
S75-p EAFsFTEsPLHKPKQ
S99 HFPGRQQSKGTCSEP
T111-p SEPSLPAtVQtAQDt
T114-p SLPAtVQtAQDtLCT
T118-p tVQtAQDtLCTTPKT
T122 AQDtLCTTPKTPtAK
T125 tLCTTPKTPtAKKLP
T127-p CTTPKTPtAKKLPVA
S142-p VFKKLEFsSsADSLS
S143 FKKLEFsSsADSLSD
S144-p KKLEFsSsADSLSDW
A164 FDMSASDAFASLAKN
S167 SASDAFASLAKNPAT
N171 AFASLAKNPATRVsT
S177-p KNPATRVsTAQKMKK
R195 NFFKPPPRKANAVKT
A197 FKPPPRKANAVKTDL
S247-p VICIDNDsAsEELTE
S249-p CIDNDsAsEELTEKD
S274 LGAERGDSEKKSHED
Y301 FEHNDNDYDIDFVPP
S309 DIDFVPPSPEEIIST
K322 STASSSLKCSSMLKD
S333 MLKDLDDSDKEKGIL
E336 DLDDSDKEKGILsTs
S341-p DKEKGILsTsEELLS
S343-p EKGILsTsEELLSKP
K349 TsEELLSKPEEMTTH
E352 ELLSKPEEMTTHKSD
S363 HKSDAGTSKDCDAQQ
A417 NIRRKLLAEAGFNGN
N424 AEAGFNGNDVRLLGS
R427 GFNGNDVRLLGSLWR
S431 NDVRLLGSLWRHRPD
S439-p LWRHRPDsLDNTVQG
S460-p HPNKELNsPYLLsHs
S465-p LNsPYLLsHsPSTEE
S467-p sPYLLsHsPSTEECL
S469 YLLsHsPSTEECLPT
T477-p TEECLPTtTPGKTGF
S504 LNSHLQKSFVSSNWA
T513 VSSNWAETPRMENRN
S544 AQSKQAASGWNVERH
S555 VERHGQASYDIDNFN
Y556 ERHGQASYDIDNFNI
S609 KSLSERISSAKAKFL
S654 PKHEHFQSLNFPHTK
K665 PHTKEMMKIFHKKFG
K669 EMMKIFHKKFGLHNF
K670 MMKIFHKKFGLHNFR
Y707 GGGKSLCYQLPACVS
S722 PGVTIVISPLRSLIV
Y744 SFDIPATYLTGDKTD
T774 IIKLLYVTPEKVCAS
S781 TPEKVCASNRLISTL
T838 SVPVMALTATANPRV
T840 PVMALTATANPRVQK
K871 SFNRHNLKYYVLPKK
S1133-p SKSAKVKsGIFGKGT
K1138 VKsGIFGKGTTYSRH
K1221-a SQREEVVkkCLGELT
K1222-a QREEVVkkCLGELTE
V1295 QKYSEWTVPAEDGSP
G1300 WTVPAEDGSPGARGA
S1301 TVPAEDGSPGARGAP
E1309 PGARGAPEDtEEEEE
T1311-p ARGAPEDtEEEEEEA
R1410 PVNRTFLRPSYAFs_
S1416-p LRPSYAFs_______
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