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Protein Page:
BLM (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
BLM a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2. Note: This description may include information from UniProtKB.
Protein type: EC 3.6.1.-; Helicase; DNA repair, damage; DNA replication; EC 3.6.4.12
Cellular Component: male germ cell nucleus; lateral element; nuclear chromosome; PML body; chromosome, telomeric region; nuclear matrix; pronucleus; cytoplasm; nucleolus; replication fork; nucleus
Molecular Function: G-quadruplex DNA binding; four-way junction helicase activity; ATP-dependent DNA helicase activity; protein binding; ATP-dependent 3'-5' DNA helicase activity; p53 binding; ATPase activity; ATP-dependent helicase activity; bubble DNA binding; helicase activity; single-stranded DNA binding; ATP binding
Biological Process: ATP catabolic process; negative regulation of DNA recombination; regulation of binding; positive regulation of transcription, DNA-dependent; DNA double-strand break processing; negative regulation of mitotic recombination; alpha-beta T cell differentiation; negative regulation of cell division; DNA repair; G2 phase of mitotic cell cycle; protein oligomerization; double-strand break repair via homologous recombination; DNA duplex unwinding; replication fork processing; DNA recombination; DNA strand renaturation; replication fork protection; positive regulation of alpha-beta T cell proliferation; regulation of cyclin-dependent protein kinase activity; G2/M transition DNA damage checkpoint; response to DNA damage stimulus; telomere maintenance; response to X-ray
Reference #:  P54132 (UniProtKB)
Alt. Names/Synonyms: BLM; Bloom syndrome protein; Bloom syndrome, RecQ helicase-like; BS; DNA helicase, RecQ-like type 2; MGC126616; MGC131618; MGC131620; RecQ protein-like 3; RECQ2; RECQL2; RECQL3
Gene Symbols: BLM
Molecular weight: 159,000 Da
Basal Isoelectric point: 7.33  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

BLM

Protein Structure Not Found.


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Sites Implicated In
apoptosis, inhibited: T99‑p, T122‑p
cell cycle regulation: T99‑p, T122‑p, S144‑p
intracellular localization: S646‑p
molecular association, regulation: T99‑p, S646‑p
protein conformation: T122‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 T20-p LERHSARtLNNKLSL
0 2 S28-p LNNKLSLsKPKFSGF
0 2 T36-p KPKFSGFtFKKKTSs
0 1 S43-p tFKKKTSsDNNVsVt
0 4 S48-p TSsDNNVsVtNVsVA
0 2 T50-p sDNNVsVtNVsVAKt
0 3 S53-p NVsVtNVsVAKtPVL
0 6 T57-p tNVsVAKtPVLRNKD
0 5 T68-p RNKDVNVtEDFsFsE
0 9 S72-p VNVtEDFsFsEPLPN
0 1 S74-p VtEDFsFsEPLPNTT
0 2 - gap
4 0 T99-p NAPAGQEtQRGGSkS
1 0 K105-ub EtQRGGSkSLLPDFL
0 1 - gap
0 3 T114-p LLPDFLQtPKEVVCT
0 1 - gap
3 1 T122-p PKEVVCTtQNtPtVK
0 5 T125-p VVCTtQNtPtVKKsR
0 2 T127-p CTtQNtPtVKKsRDT
0 1 S131-p NtPtVKKsRDTALKK
0 1 S142-p ALKKLEFsssPDSLS
0 1 S143-p LKKLEFsssPDSLST
1 6 S144-p KKLEFsssPDSLSTI
0 2 S168-p DTSETSKsFVtPPQs
0 10 T171-p ETSKsFVtPPQsHFV
0 1 S175-p sFVtPPQsHFVRVST
0 1 S181 QsHFVRVSTAQKSKK
0 7 Y199-p NFFKAQLyTtNTVKT
0 1 T201-p FKAQLyTtNTVKTDL
1 0 K225-ub IDLTEEQkDDSEWLS
0 1 - gap
0 1 - gap
1 0 K259-ub AQESDSLkTHLEDER
0 2 S269-p LEDERDNsEKKKNLE
0 1 S282-p LEEAELHsTEKVPCI
0 1 Y296-p IEFDDDDyDTDFVPP
1 3 S304-p DTDFVPPsPEEIISA
1 0 K317-sm SASSSSSkCLSTLKD
0 1 T327-p STLKDLDtsDRkEDV
0 3 S328-p TLKDLDtsDRkEDVL
1 0 K331-sm DLDtsDRkEDVLSTs
0 2 S336 DRkEDVLSTsKDLLS
0 1 T337 RkEDVLSTsKDLLSk
1 5 S338-p kEDVLSTsKDLLSkP
1 0 K344-sm TsKDLLSkPEkMsMQ
1 0 K347-sm DLLSkPEkMsMQELN
0 1 S349-p LSkPEkMsMQELNPE
0 4 S358-p QELNPETsTDCDARQ
0 2 T412-p NIRRKLLtEVDFNKs
0 3 S419-p tEVDFNKsDAsLLGs
0 14 S422-p DFNKsDAsLLGsLWR
0 1 S426-p sDAsLLGsLWRYRPD
0 11 S434-p LWRYRPDsLDGPMEG
0 1 S443-p DGPMEGDsCPTGNsM
0 1 S449-p DsCPTGNsMKELNFs
0 3 - gap
0 1 S456-p sMKELNFsHLPSNSV
0 2 S460 LNFsHLPSNSVsPGD
0 1 S462 FsHLPSNSVsPGDCL
0 3 S464-p HLPSNSVsPGDCLLT
0 1 P465 LPSNSVsPGDCLLTT
0 1 T472 PGDCLLTTtLGKTGF
0 1 T473-p GDCLLTTtLGKTGFS
0 8 S499-p FNTHLQKsFVsSNWA
1 0 S502-p HLQKsFVsSNWAEtP
0 11 T508-p VsSNWAEtPRLGKKN
1 6 S539-p DQNKHTAsINDLERE
0 1 S550-p LERETQPsyDIDNFD
0 2 Y551-p ERETQPsyDIDNFDI
0 1 S601-p KSVSERLsSAKTDCL
1 0 S646-p LKHERFQsLSFPHTK
0 1 K657-ac PHTKEMMkIFHkkFG
0 1 K661-ac EMMkIFHkkFGLHNF
0 1 K662-ac MMkIFHkkFGLHNFR
0 1 Y699-p GGGKSLCyQLPACVS
1 0 S714-p PGVTVVIsPLRSLIV
0 1 Y736-p SLDIPATyLTGDKTD
1 1 T766-p IIKLLYVtPEKICAs
0 1 S773-p tPEKICAsNRLISTL
0 1 T830-p SVPVMALtAtANPRV
0 1 T832-p PVMALtAtANPRVQK
0 1 K863-ac SFNRHNLkYYVLPKK
0 1 T903-p LSRRECDtMADtLQR
0 1 T907-p ECDtMADtLQRDGLA
0 1 S1128 SKSAKIQSGIFGkGS
0 1 K1133-ub IQSGIFGkGSAYSRH
0 1 S1197-p METENSSsVKKQKAL
0 1 K1216 SQREEMVKKCLGELT
0 1 K1217 QREEMVKKCLGELTE
1 1 S1290-p QKYSEWTsPAEDssP
0 3 S1295-p WTsPAEDssPGISLS
1 6 S1296-p TsPAEDssPGISLSS
0 1 S1304-p PGISLSSsRGPGRsA
0 5 S1310-p SsRGPGRsAAEELDE
0 2 - gap
0 1 S1375-p SSKTKSSsIIGSSSA
0 1 S1383-p IIGSSSAsHTSQATS
0 1 K1411-ac PINRPFLkPSYAFS_
0 1 S1417 LkPSYAFS_______
  mouse

 
K20 LQRHSARKLNNQPSL
S28 LNNQPSLSKPKSLGF
T36 KPKSLGFTFKKKTSE
S42 FTFKKKTSEGDVSVT
S47 KTSEGDVSVTSVSVV
T49 SEGDVSVTSVSVVKT
S52 DVSVTSVSVVKTPAL
T56 TSVSVVKTPALSDKD
S67 SDKDVNVSEAFsFTE
S71-p VNVSEAFsFTEsPLH
T73 VSEAFsFTEsPLHKP
S75-p EAFsFTEsPLHKPKQ
S99 HFPGRQQSKGTCSEP
- gap
T111-p SEPSLPAtVQtAQDt
T114-p SLPAtVQtAQDtLCT
T118-p tVQtAQDtLCTTPKT
T122 AQDtLCTTPKTPtAK
T125 tLCTTPKTPtAKKLP
T127-p CTTPKTPtAKKLPVA
L131 KTPtAKKLPVAVFKK
S142-p VFKKLEFsSsADSLS
S143 FKKLEFsSsADSLSD
S144-p KKLEFsSsADSLSDW
- gap
S167 SASDAFASLAKNPAT
- gap
S177-p KNPATRVsTAQKMKK
- gap
- gap
- gap
S247-p VICIDNDsAsEELTE
S249-p CIDNDsAsEELTEKD
K264 TQESQSLKAHLGAER
S274 LGAERGDSEKKSHED
S287 EDEAVFHSVQNTEYF
Y301 FEHNDNDYDIDFVPP
S309 DIDFVPPSPEEIIST
K322 STASSSLKCSSMLKD
- gap
S333-p MLKDLDDsDKEKGIL
- gap
S341-p DKEKGILstsEELLS
T342-p KEKGILstsEELLSK
S343-p EKGILstsEELLSKP
- gap
E352 ELLSKPEEMTTHKSD
T354 LSKPEEMTTHKSDAG
S363 HKSDAGTSKDCDAQQ
- gap
- gap
R427 GFNGNDVRLLGSLWR
S431 NDVRLLGSLWRHRPD
S439-p LWRHRPDsLDNTVQG
S448 DNTVQGDSCPVGHPN
- gap
S460-p HPNKELNsPYLLsHs
- gap
S465-p LNsPYLLsHsPStEE
S467-p sPYLLsHsPStEECL
S469 YLLsHsPStEECLPT
T470-p LLsHsPStEECLPTt
T477-p tEECLPTtTPGKTGF
T478 EECLPTtTPGKTGFS
S504 LNSHLQKSFVSSNWA
S507 HLQKSFVSSNWAETP
T513 VSSNWAETPRMENRN
S544 AQSKQAASGWNVERH
S555 VERHGQASYDIDNFN
Y556 ERHGQASYDIDNFNI
S609 KSLSERISSAKAKFL
S654 PKHEHFQSLNFPHTK
K665 PHTKEMMKIFHKKFG
K669 EMMKIFHKKFGLHNF
K670 MMKIFHKKFGLHNFR
Y707 GGGKSLCYQLPACVS
S722 PGVTIVISPLRSLIV
Y744 SFDIPATYLTGDKTD
T774 IIKLLYVTPEKVCAS
S781 TPEKVCASNRLISTL
T838 SVPVMALTATANPRV
T840 PVMALTATANPRVQK
K871 SFNRHNLKYYVLPKK
T911 LSRRECDTMADTLQR
T915 ECDTMADTLQREGLA
S1133-p SKSAKVKsGIFGKGT
K1138 VKsGIFGKGTTYSRH
S1202 METENSSSIKKQKAL
K1221-ac SQREEVVkkCLGELT
K1222-ac QREEVVkkCLGELTE
- gap
- gap
S1301 TVPAEDGSPGARGAP
- gap
- gap
T1311-p ARGAPEDtEEEEEEA
- gap
S1382 VTGSRSASCASQATS
R1410 PVNRTFLRPSYAFs_
S1416-p LRPSYAFs_______
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