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Protein Page:
EPS15 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
EPS15 Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits. Interacts with SGIP1. Interacts with HGS; the interaction bridges the interaction of STAM or STAM2 with EPS15. Isoform 2 interacts with HGS and AP2A2. Part of a complex at least composed of EPS15, HGS, and either STAM1 or STAM2. Binds AP2A2. Interacts with AP2B1; clathrin competes with EPS15. Binds STON2 and EPN1. Interacts (via its SH3-binding sites) with CRK. Interacts with SH3BP4/TTP. Interacts with ERBB2. Interacts with FCHO1. Interacts with FCHO2. Ubiquitously expressed. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold; Vesicle protein
Cellular Component: membrane; intracellular membrane-bound organelle; early endosome membrane; cytoplasm; plasma membrane; AP-2 adaptor complex; coated pit; cytosol
Molecular Function: identical protein binding; protein binding; calcium ion binding; polyubiquitin binding; SH3 domain binding
Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; cell proliferation; clathrin cage assembly; protein transport; vesicle organization and biogenesis; Golgi to endosome transport; endocytosis; endocytic recycling
Reference #:  P42566 (UniProtKB)
Alt. Names/Synonyms: AF-1P; AF1P; ALL1 fused gene from chromosome 1; epidermal growth factor receptor pathway substrate 15; Epidermal growth factor receptor substrate 15; EPS15; MLLT5; Protein AF-1p; Protein Eps15
Gene Symbols: EPS15
Molecular weight: 98,656 Da
Basal Isoelectric point: 4.52  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

EPS15

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 T106-p PPPRFHDtssPLLIS
0 2 S107-p PPRFHDtssPLLISG
0 8 S108-p PRFHDtssPLLISGT
0 1 S113 tssPLLISGTSAAEL
0 4 S140-p DAIFDSLsPVNGFLS
0 4 S221-p KRKTWVVsPAEKAkY
0 1 K227-m1 VsPAEKAkYDEIFLk
0 1 K227 VsPAEKAKYDEIFLk
0 1 K234-m1 kYDEIFLkTDKDMDG
0 2 F242 TDKDMDGFVsGLEVR
0 1 S244-p KDMDGFVsGLEVREI
0 1 T304-p IDPPHVLtPEMIPPS
0 1 S315-p IPPSDRAsLQKNIIG
0 2 I321 AsLQKNIIGssPVAD
0 5 S323-p LQKNIIGssPVADFS
0 26 S324-p QKNIIGssPVADFSA
0 1 K333-ub VADFSAIkELDTLNN
0 1 K357-ac NNVEQDLkEkEDTIK
0 1 K359-ac VEQDLkEkEDTIKQR
0 1 K386 RENTNLQKLQAQKQQ
0 1 K413-ub AQLEEQLkEVRKKCA
0 1 S435-p SLKAELTsQESQIST
0 1 Y443-p QESQISTyEEELAKA
0 1 K449 TyEEELAKAREELsR
0 1 S455-p AKAREELsRLQQETA
0 2 S467-p ETAELEEsVEsGKAQ
0 2 S470-p ELEEsVEsGKAQLEP
0 1 K472 EEsVEsGKAQLEPLQ
0 9 S485-p LQQHLQDsQQEISSM
0 1 K495-ub EISSMQMkLMEMKDL
0 1 S551-p EGQSNLEsEPIHQEs
0 1 S558-p sEPIHQEsPARssPE
0 5 S562-p HQEsPARssPELLPS
0 5 S563-p QEsPARssPELLPSG
0 2 S595-p ELDNNRHsKEEDPFN
0 1 S605-p EDPFNVDssSLTGPV
0 1 S606-p DPFNVDssSLTGPVA
0 2 K638-ub FKDDPFGkIDPFGGD
0 16 K648-ub PFGGDPFkGSDPFAs
0 1 S655-p kGSDPFAsDCFFRQS
0 1 T663-p DCFFRQStDPFATSS
0 1 S681-p FSAANNSsITSVETL
0 1 S719-p ASVFGNEsFGGGFAD
0 1 S741-p NNEDPFRsATSSsVS
0 1 S746-p FRsATSSsVSNVVIT
0 9 K754-ub VSNVVITkNVFEETS
0 5 K763-ub VFEETSVkSEDEPPA
0 16 T777-p ALPPKIGtPtRPCPL
0 2 T779-p PPKIGtPtRPCPLPP
0 3 K788-ac PCPLPPGkRsINkLD
0 2 K788-ub PCPLPPGkRsINkLD
0 11 S790-p PLPPGkRsINkLDsP
0 16 K793-ub PGkRsINkLDsPDPF
1 33 S796-p RsINkLDsPDPFkLN
0 1 P797 sINkLDsPDPFkLND
0 23 K801-ub LDsPDPFkLNDPFQP
0 34 S814-p QPFPGNDsPkEkDPE
0 13 K816-ub FPGNDsPkEkDPEIF
0 56 K818-ub GNDsPkEkDPEIFCD
2 38 Y849-p NFANFSAyPsEEDMI
0 2 S851-p ANFSAyPsEEDMIEW
0 8 K860-ub EDMIEWAkRESEREE
0 5 K890-ub ELAIALSkSEISEA_
  mouse

 
S106-p PPPRFHDsssPLLTs
S107-p PPRFHDsssPLLTsG
S108-p PRFHDsssPLLTsGP
S113-p sssPLLTsGPSVAEL
S140-p DAIFDSLsPVDGFLS
S221-p KRKTWVVsPAEKAkY
K227 VsPAEKAKYDEIFLK
K227-ub VsPAEKAkYDEIFLK
K234 kYDEIFLKTDKDMDG
Y242-p TDKDMDGyVSGLEVR
S244 KDMDGyVSGLEVRET
T304 IDPPHSLTPEMIPPS
S315 IPPSDRSSLQKNItG
T321-p SSLQKNItGSsPVAD
S323 LQKNItGSsPVADFS
S324-p QKNItGSsPVADFSA
K333 VADFSAIKELDTLNN
K357 NNVEQDLKEKEDTVK
K359 VEQDLKEKEDTVKQR
K386-ub RESINLQkLQAQKQQ
Q413 AQLEEQLQEVRKKCA
S435 SLKAEITSQESQISS
Y443 QESQISSYEEELLkA
K449-ub SYEEELLkAREELSR
S455 LkAREELSRLQQETA
S467 ETAQLEESVESGkAQ
S470 QLEESVESGkAQLEP
K472-ub EESVESGkAQLEPLQ
S485 LQQHLQESQQEISSM
R495 EISSMQMRLEMKDLE
S550 EGQNNLESEPTHQES
S557 SEPTHQESSVRssPE
S561-p HQESSVRssPEIAPS
S562-p QESSVRssPEIAPSD
S595 RFDDDKHSKEEDPFN
S605 EDPFNVESSSLTDAV
S606 DPFNVESSSLTDAVA
K638-ub FKDDPFGkIDPFGGD
K648-ub PFGGDPFkGSDPFAS
S655 kGSDPFASDCFFKQT
T664 CFFKQTSTDPFTTSS
S682 FSASSNSSNTSVETW
S721 ASVFGNESFGDGFAD
P743 NNEDAFNPTISSSTS
S748 FNPTISSSTSSVTIA
K756 TSSVTIAKPMLEETA
K765 MLEETASKSEDVPPA
T779-p ALPPKVGtPtRPCPP
T781-p PPKVGtPtRPCPPPP
K790 PCPPPPGKRPINkLD
K790 PCPPPPGKRPINkLD
P792 PPPPGKRPINkLDss
K795-ub PGKRPINkLDssDPL
S798-p RPINkLDssDPLkLN
S799-p PINkLDssDPLkLND
K803-ub LDssDPLkLNDPFQP
S816-p QPFPGNDsPkEkDPD
K818-ub FPGNDsPkEkDPDMF
K820-ub GNDsPkEkDPDMFCD
Y850-p NFANFSAyPsEEDMI
S852-p ANFSAyPsEEDMIEW
K861 EDMIEWAKRESEREE
K891 ELAIALSKSEISEA_
  rat

 
S106 PPPRFHDSSSPLLTS
S107 PPRFHDSSSPLLTSG
S108 PRFHDSSSPLLTSGT
S113 SSSPLLTSGTSVAEL
S140 DAIFDSLSPVDGFLS
S221 KRKTWVVSPAEKAKY
K227 VSPAEKAKYDEIFLK
K227 VSPAEKAKYDEIFLK
K234 KYDEIFLKTDKDMDG
Y242 TDKDMDGYVSGLEVR
S244 KDMDGYVSGLEVRET
T304 IDPPHSLTPEMIPPS
S315 IPPSDRSSLQKNTIG
I321 SSLQKNTIGSsPVAD
S323 LQKNTIGSsPVADFS
S324-p QKNTIGSsPVADFSA
K333 VADFSAIKELDTLNN
K357 NNVEQDLKEKEDTVR
K359 VEQDLKEKEDTVRQR
K386 RESLNLQKLQAQKQQ
K413 AQLEEQLKEVRKKCA
S435 SLKAEITSQESQIST
Y443 QESQISTYEEELSKA
K449 TYEEELSKAREELSR
S455 SKAREELSRLQQETA
S467 ETAQLEESVESGKAQ
S470 QLEESVESGKAQLEP
K472 EESVESGKAQLEPLQ
S485 LQQHLQDSQQEISSM
R495 EISSMQMRLAMKDLE
S550 EGQNNLESEPIHPES
S557 SEPIHPESSVRssPE
S561-p HPESSVRssPEIAPS
S562-p PESSVRssPEIAPSD
S595 RFDDDKHSKEEDPFN
S605 EDPFNVESSSLTDAV
S606 DPFNVESSSLTDAVA
K638 FKDDPFGKIDPFGGD
K648 PFGGDPFKGSDPFAS
S655 KGSDPFASDCFFKQT
T664 CFFKQTSTDPFATSS
S682 FSASSNSSNTSVETW
S720 ASVFGNESFGDGFAD
P742 SNEDPFNPTISSSAS
S747 FNPTISSSASSVVIP
K755 ASSVVIPKPVLEETP
K764 VLEETPSKSEDVPPA
T778 ALPPKTGTPTRPCPP
T780 PPKTGTPTRPCPPPP
K789 PCPPPPGKRPINKLD
K789 PCPPPPGKRPINKLD
P791 PPPPGKRPINKLDss
K794 PGKRPINKLDssDPF
S797-p RPINKLDssDPFKLN
S798-p PINKLDssDPFKLND
K802 LDssDPFKLNDPFQP
S815-p QPFPGNDsPKEKDPD
K817 FPGNDsPKEKDPDMF
K819 GNDsPKEKDPDMFCD
Y850 NFANFSAYPsEEDMI
S852-p ANFSAYPsEEDMIEW
K861 EDMIEWAKRESEREE
K891 ELAIALSKSEISEA_
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