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Protein Page:
EPS8 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
EPS8 an adaptor protein that is an epidermal growth factor receptor (EGFR) substrate. Enhances EGF-dependent mitogenic signals. Member of a family of actin barbing and capping proteins. Promotes filopodial protrusions. Complexes containing Eps8/Abi1/PI3K/Sos-1 mediate Rac activation following EGFR signaling. Involved in endocytosis of EGFR by binding to the Rab5-specific GTPase activating protein USP6N. Binds to IRSp53, which binds small the small GTPases Rac and Cdc42, and which regulates actin dynamics. Involved in the actin-remodeling activity in cultured cerebellar neurons. Regulates the actin-remodeling activities of NMDA and ethanol in neurons. Eps8 knockout mice show behavioral resistance to ethanol. Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold
Cellular Component: stereocilium; growth cone; postsynaptic density; cell cortex; cell junction; N-methyl-D-aspartate selective glutamate receptor complex
Molecular Function: protein binding; SH3/SH2 adaptor activity; Rac GTPase binding; actin binding
Biological Process: actin crosslink formation; epidermal growth factor receptor signaling pathway; actin filament bundle formation; positive regulation of signal transduction; adult locomotory behavior; Rac protein signal transduction; behavioral response to ethanol; signal transduction; regulation of cell shape; cell proliferation; actin cytoskeleton reorganization; regulation of actin filament length; barbed-end actin filament capping; exit from mitosis
Reference #:  Q12929 (UniProtKB)
Alt. Names/Synonyms: Epidermal growth factor receptor kinase substrate 8; epidermal growth factor receptor pathway substrate 8; EPS8
Gene Symbols: EPS8
Molecular weight: 91,882 Da
Basal Isoelectric point: 7.1  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

EPS8

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 Y22-p YPSQMNGyGSsPTFS
0 1 S25-p QMNGyGSsPTFSQTD
0 43 Y45-p KTSAKALyEQRKNyA
0 2 Y51-p LyEQRKNyARDsVss
1 2 S55-p RKNyARDsVssVSDI
1 0 S57-p NyARDsVssVSDISQ
1 2 S58-p yARDsVssVSDISQY
0 1 K188-ac ESAISDSkGGkQkRR
0 1 K191-ac ISDSkGGkQkRRPDA
0 1 K193-ac DSkGGkQkRRPDALR
1 1 S203-p PDALRMIsNADPSIP
0 2 T223-p PAPAPPGtVTQVDVR
0 1 S231-p VTQVDVRsRVAAWSA
0 1 K248-ub ADQGDFEkPRQyHEQ
1 5 Y252-p DFEkPRQyHEQEETP
1 1 S295-p QKAAEAFsELsKRKk
1 0 S298-p AEAFsELsKRKkNKk
0 1 K302-ac sELsKRKkNKkGKRK
0 1 K305-ac sKRKkNKkGKRKGPG
0 9 T317-p GPGEGVLtLRAKPPP
0 1 Y429-p KEQFIPPyVPRFRNG
0 1 Y454-p ATMEQDLyQLAESVA
0 21 S476-p KQEIKRLstEHssVs
0 6 T477-p QEIKRLstEHssVsE
0 8 S480-p KRLstEHssVsEyHP
0 11 S481-p RLstEHssVsEyHPA
0 27 S483-p stEHssVsEyHPADG
1 147 Y485-p EHssVsEyHPADGyA
0 157 Y491-p EyHPADGyAFssNIy
0 5 F493 HPADGyAFssNIytR
0 28 S494-p PADGyAFssNIytRG
0 10 S495-p ADGyAFssNIytRGs
0 92 Y498-p yAFssNIytRGsHLD
0 11 T499-p AFssNIytRGsHLDQ
1 0 S502-p sNIytRGsHLDQGEA
1 389 Y525-p NRHIDRNyEPLKtQP
0 1 T530-p RNyEPLKtQPKKYAK
1 29 Y540-p KKYAKSKyDFVARNN
0 1 N547 yDFVARNNsELSVLK
1 0 S548-p DFVARNNsELSVLKD
1 2 S574-p WWKVRNAsGDsGFVP
1 1 S577-p VRNAsGDsGFVPNNI
1 3 Y602-p LGRADPPytHTIQKQ
0 4 T603-p GRADPPytHTIQKQR
0 2 T620-p YGPRPADtPPAPsPP
0 2 P622 PRPADtPPAPsPPPt
1 8 S625-p ADtPPAPsPPPtPAP
1 2 T629-p PAPsPPPtPAPVPVP
0 1 T641-p PVPLPPStPAPVPVS
0 17 T655-p SKVPANItRQNssss
1 132 S659-p ANItRQNssssDsGG
1 24 S660-p NItRQNssssDsGGs
0 33 S661-p ItRQNssssDsGGsI
0 71 S662-p tRQNssssDsGGsIV
0 13 S664-p QNssssDsGGsIVRD
1 3 S667-p sssDsGGsIVRDsQR
0 5 S672-p GGsIVRDsQRHKQLP
0 1 K684 QLPVDRRKsQMEEVQ
1 30 S685-p LPVDRRKsQMEEVQD
0 3 Y723-p VPVINITyDSTPEDV
0 1 T743-p SKGFNPVtVNSLGVL
0 1 T765-p LNKDELRtVCPEGAR
1 280 Y774-p CPEGARVysQITVQk
1 0 S775-p PEGARVysQITVQkA
0 1 K781-ub ysQITVQkAALEDsS
1 0 S787-p QkAALEDsSGssELQ
1 0 S790-p ALEDsSGssELQEIM
0 1 S791-p LEDsSGssELQEIMR
0 9 S811-p ISAAASDsGVEsFDE
0 12 S815-p ASDsGVEsFDEGSsH
0 1 S821-p EsFDEGSsH______
  mouse

 
Y22 YPSQLNGYGSsPPYS
S25-p QLNGYGSsPPYSQMD
Y45-p RTSAKALyEQRKNYA
Y51 LyEQRKNYARDsVSs
S55-p RKNYARDsVSsVSDV
S57 NYARDsVSsVSDVSQ
S58-p YARDsVSsVSDVSQY
K188 ESAISDSKGGKQKRR
K191 ISDSKGGKQKRRPEA
K193 DSKGGKQKRRPEALR
A203 PEALRMIAKADPGIP
T223 PAPVPPGTVTQVDVR
S231 VTQVDVRSRVAAWSA
K248 ADQGDFEKPRQYHEQ
Y252 DFEKPRQYHEQEETP
S295-p QKAAEAFsELSKRKK
S298 AEAFsELSKRKKSKK
K302 sELSKRKKSKKSKRK
K305 SKRKKSKKSKRKGPG
T317-p GPGEGVLtLRAKPPP
Y429 KEQFIPPYVPRFRNG
Y454 APTEQDMYQLAESVA
S475-p KQDSKRLstEHsNVS
T476-p QDSKRLstEHsNVSD
S479-p KRLstEHsNVSDyPP
N480 RLstEHsNVSDyPPA
S482 stEHsNVSDyPPADG
Y484-p EHsNVSDyPPADGyA
Y490-p DyPPADGyAySSSMy
Y492-p PPADGyAySSSMyHR
S493 PADGyAySSSMyHRG
S494 ADGyAySSSMyHRGP
Y497-p yAySSSMyHRGPHAD
H498 AySSSMyHRGPHADH
P501 SSMyHRGPHADHGEA
Y524-p NHQVDRNyDAVKTQP
T529 RNyDAVKTQPKKYAK
Y539 KKYAKSKYDFVARNs
S546-p YDFVARNsSELSVMK
S547 DFVARNsSELSVMKD
S573-p WWKVRNAsGDSGFVP
S576 VRNAsGDSGFVPNNI
Y601 VGRADPPYTHTIQKQ
T602 GRADPPYTHTIQKQR
T619-p YGLRSADtPsAPsPP
S621-p LRSADtPsAPsPPPt
S624-p ADtPsAPsPPPtPAP
T628-p sAPsPPPtPAPVPVP
V640 PVPLPPSVPAPVSVP
T654-p PKVPANVtRQNssss
S658-p ANVtRQNssssDsGG
S659-p NVtRQNssssDsGGS
S660-p VtRQNssssDsGGSI
S661-p tRQNssssDsGGSIV
S663-p QNssssDsGGSIVRD
S666 sssDsGGSIVRDSQR
S671 GGSIVRDSQRYKQLP
K683-ub QLPVDRRksQMEEVQ
S684-p LPVDRRksQMEEVQD
Y722 VPVINITYDSSPEEV
T742 SKGFNPVTVNSLGVL
S764 LNKDELRSVCPEGAR
F773 CPEGARVFNQITVQK
N774 PEGARVFNQITVQKA
K780 FNQITVQKAALEDSN
S786 QKAALEDSNGSSELQ
S789 ALEDSNGSSELQEIM
S790 LEDSNGSSELQEIMR
S810-p ISAAASDsGVEsFDE
S814-p ASDsGVEsFDEGSSH
S820 EsFDEGSSH______
  rat

 
Y22 YPSQMNGYGSSPPYS
S25 QMNGYGSSPPYSQMD
Y45 RTSAKALYEQRKNYA
Y51 LYEQRKNYARDSVSS
S55 RKNYARDSVSSVSDV
S57 NYARDSVSSVSDVSQ
S58 YARDSVSSVSDVSQY
K188 ESAISDSKGGKQKRR
K191 ISDSKGGKQKRRPEA
K193 DSKGGKQKRRPEALR
A203 PEALRMIAKADPGIP
T223 PAPVPPGTVTQVDVR
S231 VTQVDVRSRVAAWSA
K248 ADQGDFEKPRQYHEQ
Y252 DFEKPRQYHEQEETP
S295 QKAAEAFSELSKRKK
S298 AEAFSELSKRKKSKK
K302 SELSKRKKSKKSKRK
K305 SKRKKSKKSKRKGPG
T317-p GPGEGVLtLRAKPPP
Y429 KEQFIPPYVPRFRNG
Y454 APTEQDMYQLAESVA
S476 KQDSKRQSTEHSSMS
T477 QDSKRQSTEHSSMSD
S480 KRQSTEHSSMSDYPP
S481 RQSTEHSSMSDYPPA
S483 STEHSSMSDYPPADG
Y485 EHSSMSDYPPADGYT
Y491 DYPPADGYTFSNSMY
F493 PPADGYTFSNSMYHR
S494 PADGYTFSNSMYHRG
N495 ADGYTFSNSMYHRGP
Y498 YTFSNSMYHRGPHVD
H499 TFSNSMYHRGPHVDQ
P502 NSMYHRGPHVDQGEA
Y525 NRHVDRNYDPVKTQP
T530 RNYDPVKTQPKKYAK
Y540 KKYAKSKYDFVARNS
S547 YDFVARNSSELSVMK
S548 DFVARNSSELSVMKD
S574 WWKVRNASGDSGFVP
S577 VRNASGDSGFVPNNI
Y602 VGRTDPPYTHTIQKQ
T603 GRTDPPYTHTIQKQR
T620 YGPRSADTPSAPSPP
S622 PRSADTPSAPSPPPT
S625 ADTPSAPSPPPTPAP
T629 SAPSPPPTPAPVPVP
A641 PVPLPPSAPAPVPVP
T655 PKVPANVTRQNssss
S659-p ANVTRQNssssESGG
S660-p NVTRQNssssESGGS
S661-p VTRQNssssESGGSI
S662-p TRQNssssESGGSIA
S664 QNssssESGGSIARD
S667 sssESGGSIARDsQR
S672-p GGSIARDsQRYKQLP
K684 QLPVDRRKSQMEEVQ
S685 LPVDRRKSQMEEVQD
Y723 VPVINITYDSSPEEV
T743 SKGFNPVTVSSLGVL
S765 LNKDELRSVCPEGAR
F774 CPEGARVFSQITVQK
S775 PEGARVFSQITVQKA
K781 FSQITVQKAALEDSS
S787 QKAALEDSSGSSELQ
S790 ALEDSSGSSELQEIM
S791 LEDSSGSSELQEIMR
S811 ISAAASDSGVEsFDE
S815-p ASDSGVEsFDEGSSH
S821 EsFDEGSSH______
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