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DARPP-32 (human)

Overview DARPP-32 a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32. Two alternatively-spliced isoforms have been described. Note: This description may include information from UniProtKB. Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis Cellular Component: cytoplasm; cytosol Molecular Function: protein kinase inhibitor activity; protein phosphatase type 1 regulator activity; protein phosphatase inhibitor activity Biological Process: negative regulation of protein kinase activity; signal transduction Reference #:  Q9UD71 (UniProtKB) Alt. Names/Synonyms: DARPP-32; DARPP32; dopamine and cAMP regulated phosphoprotein; dopamine and cAMP-regulated neuronal phosphoprotein 32; Dopamine- and cAMP-regulated neuronal phosphoprotein; FLJ20940; PPP1R1B; PPR1B; Protein phosphatase 1 regulatory subunit 1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B (dopamine and cAMP regulated phosphoprotein, DARPP-32) Gene Symbols: PPP1R1B Molecular weight: 22,963 Da Basal Isoelectric point: 4.48  Predict pI for various phosphorylation states CST Pathways:  Parkinson's Disease Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below DARPP-32

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	Sites Implicated In
cell motility, altered: T34‑p cytoskeletal reorganization: T34‑p

	Modification Sites and Domains

	Modification Sites in Parent Protein, Orthologs, and Isoforms

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	2		S12-p	DRKKIQFsVPAPPSQ
53	45		T34-p	MIRRRRPtPAMLFRL
1	0		S42	PAMLFRLSEHssPEE
2	8		S45-p	LFRLSEHssPEEEAS
0	11		S46-p	FRLSEHssPEEEASP
0	45		Y74-p	KRPNPCAytPPsLKA
27	18		T75-p	RPNPCAytPPsLKAV
0	3		S78-p	PCAytPPsLKAVQRI
1	1		N97	LQSISNLNENQAsEE
6	26		S102-p	NLNENQAsEEEDELG
0	2		Y116-p	GELRELGyPREEDEE
13	9		S137-p	EEEEEEDsQAEVLKV
0	7		S198	GEEPQRPSPSEPGT_

	mouse
S12	DRKKIQFSVPAPPSQ
T34-p	MIRRRRPtPAMLFRV
S42	PAMLFRVSEHssPEE
S45-p	LFRVSEHssPEEEAS
S46-p	FRVSEHssPEEEASP
Y74	KRPNPCAYtPPSLKA
T75-p	RPNPCAYtPPSLKAV
S78	PCAYtPPSLKAVQHL
S92-p	LQTISNLsENQAsEE
S97-p	NLsENQAsEEEDELG
Y111	GELRELGYPQEDDEE
S130-p	EEDEEEDsQAEVLKG
S192-p	GEEPQHPsPP_____

	rat
S12	DRKKIQFSVPAPPSQ
T34-p	MIRRRRPtPALLFRV
S42	PALLFRVSEHsSPEE
S45-p	LFRVSEHsSPEEESS
S46	FRVSEHsSPEEESSP
Y74	KRPNPCAYtPPSLKA
T75-p	RPNPCAYtPPSLKAV
S78	PCAYtPPSLKAVQRI
S97-p	LQTISNLsENQAsEE
S102-p	NLsENQAsEEEDELG
Y116	GELRELGYPQEDDEE
S137-p	EEDEEEDsQAEVLKG
T199	GEEPRHPTPPESGT_

	cow
S12	DRKKIQFSVPAPPSQ
T34-p	MIRRRRPtPAMLFRL
S42-p	PAMLFRLsEHsSPEE
S45-p	LFRLsEHsSPEEEAS
S46	FRLsEHsSPEEEASP
Y74	KRSNPCAYTPPSLKA
T75	RSNPCAYTPPSLKAV
S78	PCAYTPPSLKAVQRI
G97	LQSISNLGENQAsEE
S102-p	NLGENQAsEEEDELG
Y116	GELRELGYPREEEEE
S135	DEEEEEDSQAEVLKG
A196	GEEPQRPAHPEPGT_

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