Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
DARPP-32 (human)

Overview
DARPP-32 a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32. Two alternatively-spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis
Cellular Component: cell soma; cytosol; nucleus
Molecular Function: protein kinase inhibitor activity; protein phosphatase type 1 regulator activity; D4 dopamine receptor binding; protein phosphatase inhibitor activity; D1 dopamine receptor binding; D3 dopamine receptor binding; D2 dopamine receptor binding; D5 dopamine receptor binding
Biological Process: negative regulation of female receptivity; transcription, DNA-dependent; regulation of catalytic activity; response to amphetamine; negative regulation of catalytic activity; negative regulation of protein kinase activity; visual learning; signal transduction
Reference #:  Q9UD71 (UniProtKB)
Alt. Names/Synonyms: DARPP-32; DARPP32; dopamine and cAMP regulated phosphoprotein; dopamine and cAMP-regulated neuronal phosphoprotein 32; Dopamine- and cAMP-regulated neuronal phosphoprotein; FLJ20940; PPP1R1B; PPR1B; Protein phosphatase 1 regulatory subunit 1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B (dopamine and cAMP regulated phosphoprotein, DARPP-32)
Gene Symbols: PPP1R1B
Molecular weight: 22,963 Da
Basal Isoelectric point: 4.48  Predict pI for various phosphorylation states
CST Pathways:  Parkinson's Disease
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

DARPP-32

Protein Structure Not Found.


STRING  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Scansite  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
cell motility, altered: T34‑p
cytoskeletal reorganization: T34‑p

Modification Sites and Domains  

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 S12-p DRKKIQFsVPAPPSQ
54 46 T34-p MIRRRRPtPAMLFRL
1 0 S42 PAMLFRLSEHssPEE
2 8 S45-p LFRLSEHssPEEEAS
0 11 S46-p FRLSEHssPEEEASP
0 51 Y74-p KRPNPCAytPPsLKA
27 18 T75-p RPNPCAytPPsLKAV
0 3 S78-p PCAytPPsLKAVQRI
1 1 N97 LQSISNLNENQAsEE
6 28 S102-p NLNENQAsEEEDELG
0 1 Y116-p GELRELGyPREEDEE
13 10 S137-p EEEEEEDsQAEVLKV
0 7 S198 GEEPQRPSPSEPGT_
5393 : Phospho-DARPP-32 (Thr34) (D29E8) Rabbit mAb
12438 : Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb
2301 : Phospho-DARPP-32 (Thr75) Antibody
3401 : Phospho-DARPP-32 (Ser97) (D11A5) Rabbit mAb
  mouse

 
S12 DRKKIQFSVPAPPSQ
T34-p MIRRRRPtPAMLFRV
S42 PAMLFRVSEHssPEE
S45-p LFRVSEHssPEEEAS
S46-p FRVSEHssPEEEASP
Y74 KRPNPCAYtPPSLKA
T75-p RPNPCAYtPPSLKAV
S78 PCAYtPPSLKAVQHL
S92-p LQTISNLsENQAsEE
S97-p NLsENQAsEEEDELG
Y111 GELRELGYPQEDDEE
S130-p EEDEEEDsQAEVLKG
S192-p GEEPQHPsPP_____
5393 : Phospho-DARPP-32 (Thr34) (D29E8) Rabbit mAb
12438 : Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb
2301 : Phospho-DARPP-32 (Thr75) Antibody
3401 : Phospho-DARPP-32 (Ser97) (D11A5) Rabbit mAb
  rat

 
S12 DRKKIQFSVPAPPSQ
T34-p MIRRRRPtPALLFRV
S42 PALLFRVSEHssPEE
S45-p LFRVSEHssPEEESS
S46-p FRVSEHssPEEESSP
Y74 KRPNPCAYtPPSLKA
T75-p RPNPCAYtPPSLKAV
S78 PCAYtPPSLKAVQRI
S97-p LQTISNLsENQAsEE
S102-p NLsENQAsEEEDELG
Y116 GELRELGYPQEDDEE
S137-p EEDEEEDsQAEVLKG
T199 GEEPRHPTPPESGT_
5393 : Phospho-DARPP-32 (Thr34) (D29E8) Rabbit mAb
12438 : Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb
2301 : Phospho-DARPP-32 (Thr75) Antibody
3401 : Phospho-DARPP-32 (Ser97) (D11A5) Rabbit mAb
  cow

 
S12 DRKKIQFSVPAPPSQ
T34-p MIRRRRPtPAMLFRL
S42-p PAMLFRLsEHsSPEE
S45-p LFRLsEHsSPEEEAS
S46 FRLsEHsSPEEEASP
Y74 KRSNPCAYTPPSLKA
T75 RSNPCAYTPPSLKAV
S78 PCAYTPPSLKAVQRI
G97 LQSISNLGENQAsEE
S102-p NLGENQAsEEEDELG
Y116 GELRELGYPREEEEE
S135 DEEEEEDSQAEVLKG
A196 GEEPQRPAHPEPGT_
5393 : Phospho-DARPP-32 (Thr34) (D29E8) Rabbit mAb
12438 : Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.