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Protein Page:
Rb (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
Rb retinoblastoma tumor suppressor protein regulates cell proliferation by controlling progression through the G1-phase restriction point. Has three distinct binding domains and interacts with regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by Cdks inhibits Rb target binding, thus allowing cell cycle progression. Recruits and targets histone methyltransferase suv39h1 leading to epigenetic transcriptional repression. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; Tumor suppressor; Transcription, coactivator/corepressor
Cellular Component: nucleoplasm; SWI/SNF complex; PML body; Rb-E2F complex; spindle; nucleus; chromatin
Molecular Function: identical protein binding; protein binding; androgen receptor binding; DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; phosphoprotein binding; transcription factor binding; kinase binding; transcription factor activity
Biological Process: viral reproduction; sister chromatid biorientation; negative regulation of transcription from RNA polymerase II promoter, mitotic; positive regulation of transcription, DNA-dependent; regulation of mitotic cell cycle; neuron maturation; positive regulation of macrophage differentiation; cell cycle arrest; neurite development; negative regulation of epithelial cell proliferation; transcription, DNA-dependent; negative regulation of transcription factor activity; mitotic cell cycle checkpoint; regulation of lipid kinase activity; enucleate erythrocyte differentiation; myoblast differentiation; G1/S-specific transcription in mitotic cell cycle; chromatin remodeling; neuron apoptosis; maintenance of mitotic sister chromatid cohesion; gut development; neuron morphogenesis during differentiation; negative regulation of smoothened signaling pathway; androgen receptor signaling pathway; Ras protein signal transduction; positive regulation of mitotic metaphase/anaphase transition; striated muscle cell differentiation; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; cell cycle checkpoint; G1/S transition of mitotic cell cycle
Reference #:  P06400 (UniProtKB)
Alt. Names/Synonyms: OSRC; p105-Rb; pp110; pRb; Rb; RB1; retinoblastoma 1; retinoblastoma suspectibility protein; Retinoblastoma-associated protein
Gene Symbols: RB1
Molecular weight: 106,159 Da
Basal Isoelectric point: 8.13  Predict pI for various phosphorylation states
CST Pathways:  G1/S Checkpoint  |  Protein Acetylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Rb

Protein Structure Not Found.


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Sites Implicated In
apoptosis, induced: S567‑p, S612‑p
apoptosis, inhibited: S780‑p, T821‑p
cell cycle regulation: S249‑p, T252‑p, S780‑p, S795‑p, S807‑p, S811‑p, K873‑a, K874‑a
cell growth, altered: S780‑p, S788‑p, S795‑p, S807‑p, S811‑p, T821‑p, T826‑p
cell growth, induced: S795‑p
transcription, altered: S612‑p, S780‑p, S807‑p, S811‑p
activity, inhibited: S567‑p, S780‑p, S795‑p
intracellular localization: K873‑a, K874‑a
molecular association, regulation: T356‑p, T373‑p, S567‑p, S608‑p, S612‑p, S780‑p, S788‑p, S795‑p, S807‑p, S811‑p, T821‑p, T826‑p, K873‑a, K874‑a
phosphorylation: K873‑a, K874‑a
protein conformation: S608‑p, S612‑p
protein degradation: S567‑p, S780‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 0 T5-p ___MPPKtPRKTAAT
0 21 S37-p EEDPEQDsGPEDLPL
0 1 K143-u KEIDTSTkVDNAMsR
0 2 S149-p TkVDNAMsRLLKKYD
2 0 S230-p LDYFIKLsPPMLLKE
13 86 S249-p AVIPINGsPRtPRRG
14 62 T252-p PINGsPRtPRRGQNR
0 2 K265-u NRSARIAkQLENDTR
0 5 Y321-p VENLSKRyEEIyLKN
0 5 Y325-p SKRyEEIyLKNKDLD
0 1 T345-p DHDKTLQtdsIDsFE
0 1 D346-ca HDKTLQtdsIDsFEt
0 4 S347-p DKTLQtdsIDsFEtQ
0 7 S350-p LQtdsIDsFEtQRtP
0 10 T353-p dsIDsFEtQRtPRKs
8 33 T356-p DsFEtQRtPRKsNLD
0 1 S360-p tQRtPRKsNLDEEVN
1 3 P370 DEEVNVIPPHtPVRT
9 52 T373-p VNVIPPHtPVRTVMN
0 1 K427-a KDIGYIFkEKFAKAV
0 1 K548-a NLTREMIkHLERCEH
4 0 S567-p SLAWLSDsPLFDLIK
0 2 T583-p SKDREGPtDHLEsAC
0 2 S588-p GPtDHLEsACPLNLP
0 12 T601-p LPLQNNHtAADMyLs
0 12 Y606-p NHtAADMyLsPVRsP
9 34 S608-p tAADMyLsPVRsPKK
10 39 S612-p MyLsPVRsPKKKGST
0 45 S624-p GSTTRVNstANAETQ
0 5 T625-p STTRVNstANAETQA
1 0 K720-s KVKNIDLkFKIIVTA
0 7 S773-p TNILQYAstRPPtLs
0 5 T774-p NILQYAstRPPtLsP
0 61 T778-p YAstRPPtLsPIPHI
59 117 S780-p stRPPtLsPIPHIPR
9 72 S788-p PIPHIPRsPyKFPss
0 25 Y790-p PHIPRsPyKFPssPL
0 1 P793 PRsPyKFPssPLRIP
0 64 S794-p RsPyKFPssPLRIPG
37 93 S795-p sPyKFPssPLRIPGG
1 23 Y805-p RIPGGNIyIsPLksP
50 128 S807-p PGGNIyIsPLksPyK
0 2 K810-u NIyIsPLksPyKIsE
51 90 S811-p IyIsPLksPyKIsEG
0 12 Y813-p IsPLksPyKIsEGLP
0 6 S816-p LksPyKIsEGLPtPt
19 66 T821-p KIsEGLPtPtKMtPR
0 50 T823-p sEGLPtPtKMtPRSR
14 66 T826-p LPtPtKMtPRSRILV
0 2 S834-p PRSRILVsIGEsFGt
0 2 S838-p ILVsIGEsFGtsEKF
0 11 T841-p sIGEsFGtsEKFQKI
0 3 S842-p IGEsFGtsEKFQKIN
0 14 S855-p INQMVCNsDRVLKRS
3 0 K873-a SNPPKPLkkLRFDIE
3 1 K874-a NPPKPLkkLRFDIEG
0 1 S882-p LRFDIEGsDEADGSK
0 2 S895-p SKHLPGEskFQQKLA
0 1 K896-a KHLPGEskFQQKLAE
0 2 S919-p QKQKMNDsMDTSNKE
2181 : Phospho-Rb (Ser608) Antibody
8147 : Phospho-Rb (Ser608) (D10F2) Rabbit mAb
3590 : Phospho-Rb (Ser780) (C84F6) Rabbit mAb
8180 : Phospho-Rb (Ser780) (D59B7) Rabbit mAb
9307 : Phospho-Rb (Ser780) Antibody
9301 : Phospho-Rb (Ser795) Antibody
4277 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
8516 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb
8880 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8957 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 555 Conjugate)
8974 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
9308 : Phospho-Rb (Ser807/811) Antibody
11917 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (PE Conjugate)
4277 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
8516 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb
8880 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8957 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 555 Conjugate)
8974 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
9308 : Phospho-Rb (Ser807/811) Antibody
11917 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (PE Conjugate)
  mouse

 
A5 ___MPPKAPRRAAAA
S31-p EDDPAQDsGPEELPL
K137 KEIDTSTKVDNAMSR
S143 TKVDNAMSRLLKKYN
S224-p VDYFIKFsPPALLRE
S243-p AAIPINGsPRtPRRG
T246-p PINGsPRtPRRGQNR
K259 NRSARIAKQLENDTR
Y315 VESLSKRYEEVYLKN
Y319 SKRYEEVYLKNKDLD
T339 DHDKTLQTDPIDsFE
D340 HDKTLQTDPIDsFEt
P341 DKTLQTDPIDsFEtE
S344-p LQTDPIDsFEtERtP
T347-p DPIDsFEtERtPRKN
T350-p DsFEtERtPRKNNPD
N354 tERtPRKNNPDEEAN
T364-p DEEANVVtPHtPVRT
T367-p ANVVtPHtPVRTVMN
K421 KDVGHIFKEKFANAV
K542 NLTREMIKHLERCEH
S561-p SLAWLSDsPLFDLIK
- gap
P581 EGPDNLEPACPLSLP
T594-p LPLQGNHtAADMyLs
Y599-p NHtAADMyLsPLRsP
S601-p tAADMyLsPLRsPKK
S605-p MyLsPLRsPKKRTST
S617-p TSTTRVNsAANTETQ
A618 STTRVNsAANTETQA
K713 KVKNIDLKFKIIVTA
S766 TNILQYASTRPPtLs
T767 NILQYASTRPPtLsP
T771-p YASTRPPtLsPIPHI
S773-p STRPPtLsPIPHIPR
S781-p PIPHIPRsPyKFsss
Y783-p PHIPRsPyKFsssPL
S786-p PRsPyKFsssPLRIP
S787-p RsPyKFsssPLRIPG
S788-p sPyKFsssPLRIPGG
Y798-p RIPGGNIyIsPLksP
S800-p PGGNIyIsPLksPyK
K803-u NIyIsPLksPyKIsE
S804-p IyIsPLksPyKIsEG
Y806-p IsPLksPyKIsEGLP
S809-p LksPyKIsEGLPtPt
T814-p KIsEGLPtPtKMtPR
T816-p sEGLPtPtKMtPRSR
T819-p LPtPtKMtPRSRILV
S827 PRSRILVSIGESFGT
S831 ILVSIGESFGTSEKF
T834 SIGESFGTSEKFQKI
S835 IGESFGTSEKFQKIN
S848-p INQMVCNsDRVLKRS
K866 GNPPKPLKKLRFDIE
K867 NPPKPLKKLRFDIEG
A875 LRFDIEGADEADGSK
S888 SKHLPAESKFQQKLA
K889 KHLPAESKFQQKLAE
S912 QKQRMNESKDVSNKE
2181 : Phospho-Rb (Ser608) Antibody
3590 : Phospho-Rb (Ser780) (C84F6) Rabbit mAb
8180 : Phospho-Rb (Ser780) (D59B7) Rabbit mAb
9307 : Phospho-Rb (Ser780) Antibody
9301 : Phospho-Rb (Ser795) Antibody
4277 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
8516 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb
8880 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8957 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 555 Conjugate)
8974 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
9308 : Phospho-Rb (Ser807/811) Antibody
11917 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (PE Conjugate)
4277 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
8516 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb
8880 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8957 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 555 Conjugate)
8974 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
9308 : Phospho-Rb (Ser807/811) Antibody
11917 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (PE Conjugate)
  rat

 
A5 ___MPPKAPRRTAAA
S30-p EDDPAQDsDPEELPL
K136 KEIDTSTKVDNAVSR
S142 TKVDNAVSRLLKKYN
S223 LDYFIKLSPPALLRE
S242-p AATPINGsPRtPRRG
T245-p PINGsPRtPRRGQNR
K258 NRSARIAKQLESDTR
Y314 LESLSKRYEEVYLKS
Y318 SKRYEEVYLKSKDLD
T338 DHDKTLQTDTIDSFE
D339 HDKTLQTDTIDSFET
T340 DKTLQTDTIDSFETE
S343 LQTDTIDSFETERTP
T346 DTIDSFETERTPRKS
T349 DSFETERTPRKSNPD
S353 TERTPRKSNPDEEAN
T363 DEEANMVTPHtPVRT
T366-p ANMVTPHtPVRTVMN
K420 KDVGHIFKEKFASAV
K541 NLTREMIKHLERCEH
S560 SLAWLSDSPLFDLIK
- gap
S580 EGPDHLESACSLSLP
T593 LPLQSNHTAADMYLs
Y598 NHTAADMYLsPIRSP
S600-p TAADMYLsPIRSPKK
S604 MYLsPIRSPKKRTST
S616 TSTTRVNSAANTETQ
A617 STTRVNSAANTETQA
K712 KVKNIDLKFKIIVTA
S765 TNILQYASTRPPTLs
T766 NILQYASTRPPTLsP
T770 YASTRPPTLsPIPHI
S772-p STRPPTLsPIPHIPR
S780-p PIPHIPRsPYKFSSs
Y782 PHIPRsPYKFSSsPL
S785 PRsPYKFSSsPLRIP
S786 RsPYKFSSsPLRIPG
S787-p sPYKFSSsPLRIPGG
Y797 RIPGGNIYIsPLKsP
S799-p PGGNIYIsPLKsPYK
K802 NIYIsPLKsPYKISE
S803-p IYIsPLKsPYKISEG
Y805 IsPLKsPYKISEGLP
S808 LKsPYKISEGLPtPT
T813-p KISEGLPtPTKMtPR
T815 SEGLPtPTKMtPRSR
T818-p LPtPTKMtPRSRILV
S826-p PRSRILVsIGEsFGT
S830-p ILVsIGEsFGTsEKF
T833 sIGEsFGTsEKFQKI
S834-p IGEsFGTsEKFQKIN
S847 INQMVCNSDRVLKRS
K865 GNPPKPLKKLRFDIE
K866 NPPKPLKKLRFDIEG
S874 LRFDIEGSDEADGSK
S887 SKHLPAESKFQQKLA
K888 KHLPAESKFQQKLAE
S911 QKQKLNDSMEISNKE
2181 : Phospho-Rb (Ser608) Antibody
3590 : Phospho-Rb (Ser780) (C84F6) Rabbit mAb
8180 : Phospho-Rb (Ser780) (D59B7) Rabbit mAb
9307 : Phospho-Rb (Ser780) Antibody
4277 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
8516 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb
8880 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8957 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 555 Conjugate)
8974 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
9308 : Phospho-Rb (Ser807/811) Antibody
11917 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (PE Conjugate)
4277 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
8516 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb
8880 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8957 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 555 Conjugate)
8974 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
9308 : Phospho-Rb (Ser807/811) Antibody
11917 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (PE Conjugate)
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