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Protein Page:
Rb (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Rb retinoblastoma tumor suppressor protein regulates cell proliferation by controlling progression through the G1-phase restriction point. Has three distinct binding domains and interacts with regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by Cdks inhibits Rb target binding, thus allowing cell cycle progression. Recruits and targets histone methyltransferase suv39h1 leading to epigenetic transcriptional repression. Note: This description may include information from UniProtKB.
Protein type: Tumor suppressor; Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Cellular Component: nucleoplasm; SWI/SNF complex; PML body; Rb-E2F complex; spindle; chromatin; nucleus
Molecular Function: identical protein binding; protein binding; DNA binding; androgen receptor binding; ubiquitin protein ligase binding; transcription coactivator activity; phosphoprotein binding; kinase binding; transcription factor binding; transcription factor activity
Biological Process: sister chromatid biorientation; viral reproduction; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter, mitotic; regulation of mitotic cell cycle; neuron maturation; positive regulation of macrophage differentiation; cell cycle arrest; neurite development; negative regulation of epithelial cell proliferation; transcription, DNA-dependent; mitotic cell cycle checkpoint; negative regulation of transcription factor activity; regulation of lipid kinase activity; enucleate erythrocyte differentiation; G1/S-specific transcription in mitotic cell cycle; myoblast differentiation; chromatin remodeling; neuron apoptosis; maintenance of mitotic sister chromatid cohesion; gut development; neuron morphogenesis during differentiation; negative regulation of smoothened signaling pathway; androgen receptor signaling pathway; Ras protein signal transduction; positive regulation of mitotic metaphase/anaphase transition; negative regulation of protein kinase activity; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; cell cycle checkpoint; G1/S transition of mitotic cell cycle
Reference #:  P06400 (UniProtKB)
Alt. Names/Synonyms: OSRC; p105-Rb; pp110; pRb; Rb; RB1; retinoblastoma 1; retinoblastoma suspectibility protein; Retinoblastoma-associated protein
Gene Symbols: RB1
Molecular weight: 106,159 Da
Basal Isoelectric point: 8.13  Predict pI for various phosphorylation states
CST Pathways:  G1/S Checkpoint  |  Protein Acetylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Rb

Protein Structure Not Found.


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Sites Implicated In
apoptosis, induced: S567‑p, S612‑p
apoptosis, inhibited: S780‑p, T821‑p
cell cycle regulation: S249‑p, T252‑p, S780‑p, S795‑p, S807‑p, S811‑p, K873‑ac, K874‑ac
cell growth, altered: S780‑p, S788‑p, S795‑p, S807‑p, S811‑p, T821‑p, T826‑p
cell growth, induced: S795‑p
transcription, altered: S612‑p, S780‑p, S807‑p, S811‑p
activity, inhibited: S567‑p, S780‑p, S795‑p
intracellular localization: K873‑ac, K874‑ac
molecular association, regulation: T356‑p, T373‑p, S567‑p, S608‑p, S612‑p, S780‑p, S788‑p, S795‑p, S807‑p, S811‑p, T821‑p, T826‑p, K873‑ac, K874‑ac
phosphorylation: K873‑ac, K874‑ac
protein conformation: S608‑p, S612‑p
protein degradation: S567‑p, S780‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 0 T5-p ___MPPKtPRKTAAT
0 26 S37-p EEDPEQDsGPEDLPL
0 1 K63 DFTALCQKLKIPDHV
0 1 T140-p NLLKEIDtSTkVDNA
0 1 K143-ub KEIDtSTkVDNAMsR
0 2 S149-p TkVDNAMsRLLKKYD
0 1 S163-p DVLFALFsKLERTCE
2 0 S230-p LDYFIKLsPPMLLKE
0 1 Y239-p PMLLKEPyKtAVIPI
0 1 T241-p LLKEPyKtAVIPING
13 93 S249-p AVIPINGsPRtPRRG
14 66 T252-p PINGsPRtPRRGQNR
0 2 K265-ub NRSARIAkQLENDTR
0 5 Y321-p VENLSKRyEEIyLKN
0 5 Y325-p SKRyEEIyLKNKDLD
0 1 T345-p DHDKTLQtdsIDsFE
0 1 D346-ca HDKTLQtdsIDsFEt
0 4 S347-p DKTLQtdsIDsFEtQ
0 7 S350-p LQtdsIDsFEtQRtP
0 12 T353-p dsIDsFEtQRtPRKs
8 36 T356-p DsFEtQRtPRKsNLD
0 1 S360-p tQRtPRKsNLDEEVN
1 3 P370 DEEVNVIPPHtPVRT
9 55 T373-p VNVIPPHtPVRTVMN
0 1 K427-ac KDIGYIFkEKFAKAV
0 1 Y446 VEIGSQRYKLGVRLY
0 1 K548-ac NLTREMIkHLERCEH
4 0 S567-p SLAWLSDsPLFDLIk
0 1 K574-ub sPLFDLIkQSKDREG
0 2 T583-p SKDREGPtDHLEsAC
0 2 S588-p GPtDHLEsACPLNLP
0 12 T601-p LPLQNNHtAADMyLs
0 12 Y606-p NHtAADMyLsPVRsP
9 34 S608-p tAADMyLsPVRsPKK
10 40 S612-p MyLsPVRsPKKKGsT
0 1 S618-p RsPKKKGsTTRVNst
0 45 S624-p GsTTRVNstANAETQ
0 5 T625-p sTTRVNstANAETQA
1 0 K720-sm KVKNIDLkFKIIVTA
0 1 Y771-p LKTNILQyAstRPPt
0 7 S773-p TNILQyAstRPPtLs
0 9 T774-p NILQyAstRPPtLsP
0 67 T778-p yAstRPPtLsPIPHI
60 125 S780-p stRPPtLsPIPHIPR
9 73 S788-p PIPHIPRsPyKFPss
0 27 Y790-p PHIPRsPyKFPssPL
0 1 P793 PRsPyKFPssPLRIP
0 62 S794-p RsPyKFPssPLRIPG
38 92 S795-p sPyKFPssPLRIPGG
1 27 Y805-p RIPGGNIyIsPLksP
51 126 S807-p PGGNIyIsPLksPyK
0 2 K810-ub NIyIsPLksPyKIsE
1 0 K810-m1 NIyIsPLksPyKIsE
1 0 K810-m2 NIyIsPLksPyKIsE
52 89 S811-p IyIsPLksPyKIsEG
0 12 Y813-p IsPLksPyKIsEGLP
0 6 S816-p LksPyKIsEGLPtPt
19 68 T821-p KIsEGLPtPtKMtPR
0 52 T823-p sEGLPtPtKMtPRsR
14 69 T826-p LPtPtKMtPRsRILV
0 1 S829-p PtKMtPRsRILVsIG
0 2 S834-p PRsRILVsIGEsFGt
0 2 S838-p ILVsIGEsFGtsEKF
0 12 T841-p sIGEsFGtsEKFQKI
0 3 S842-p IGEsFGtsEKFQKIN
0 15 S855-p INQMVCNsDRVLKRS
3 0 K873-ac SNPPKPLkkLRFDIE
3 1 K874-ac NPPKPLkkLRFDIEG
0 2 S882-p LRFDIEGsDEADGSK
0 3 S895-p SKHLPGEskFQQKLA
0 1 K896-ac KHLPGEskFQQKLAE
0 1 T905-p QQKLAEMtSTRTRMQ
0 2 S919-p QKQKMNDsMDTSNkE
0 1 K925-ac DsMDTSNkEEK____
2181 : Phospho-Rb (Ser608) Antibody
8147 : Phospho-Rb (Ser608) (D10F2) Rabbit mAb
3590 : Phospho-Rb (Ser780) (C84F6) Rabbit mAb
8180 : Phospho-Rb (Ser780) (D59B7) Rabbit mAb
9307 : Phospho-Rb (Ser780) Antibody
9301 : Phospho-Rb (Ser795) Antibody
4277 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
8516 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb
8880 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8957 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 555 Conjugate)
8974 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
9308 : Phospho-Rb (Ser807/811) Antibody
11917 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (PE Conjugate)
4277 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
8516 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb
8880 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8957 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 555 Conjugate)
8974 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
9308 : Phospho-Rb (Ser807/811) Antibody
11917 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (PE Conjugate)
  mouse

 
A5 ___MPPKAPRRAAAA
S31-p EDDPAQDsGPEELPL
K57-ub EFIALCQkLKVPDHV
T134 DLLKEIDTSTKVDNA
K137 KEIDTSTKVDNAMSR
S143 TKVDNAMSRLLKKYN
S157 NVLCALYSKLERTCE
S224-p VDYFIKFsPPALLRE
Y233 PALLREPYKTAAIPI
T235 LLREPYKTAAIPING
S243-p AAIPINGsPRtPRRG
T246-p PINGsPRtPRRGQNR
K259 NRSARIAKQLENDTR
Y315 VESLSKRYEEVYLKN
Y319 SKRYEEVYLKNKDLD
T339 DHDKTLQTDPIDsFE
D340 HDKTLQTDPIDsFEt
P341 DKTLQTDPIDsFEtE
S344-p LQTDPIDsFEtERtP
T347-p DPIDsFEtERtPRKN
T350-p DsFEtERtPRKNNPD
N354 tERtPRKNNPDEEAN
T364-p DEEANVVtPHtPVRT
T367-p ANVVtPHtPVRTVMN
K421 KDVGHIFKEKFANAV
Y440-p VDIGVQRyKLGVRLY
K542 NLTREMIKHLERCEH
S561-p SLAWLSDsPLFDLIK
K568 sPLFDLIKQSKDGEG
- gap
P581 EGPDNLEPACPLSLP
T594-p LPLQGNHtAADMyLs
Y599-p NHtAADMyLsPLRsP
S601-p tAADMyLsPLRsPKK
S605-p MyLsPLRsPKKRTST
S611 RsPKKRTSTTRVNsA
S617-p TSTTRVNsAANTETQ
A618 STTRVNsAANTETQA
K713 KVKNIDLKFKIIVTA
Y764-p LKTNILQyASTRPPt
S766 TNILQyASTRPPtLs
T767 NILQyASTRPPtLsP
T771-p yASTRPPtLsPIPHI
S773-p STRPPtLsPIPHIPR
S781-p PIPHIPRsPyKFsss
Y783-p PHIPRsPyKFsssPL
S786-p PRsPyKFsssPLRIP
S787-p RsPyKFsssPLRIPG
S788-p sPyKFsssPLRIPGG
Y798-p RIPGGNIyIsPLksP
S800-p PGGNIyIsPLksPyK
K803-ub NIyIsPLksPyKIsE
K803 NIyIsPLKsPyKIsE
K803 NIyIsPLKsPyKIsE
S804-p IyIsPLksPyKIsEG
Y806-p IsPLksPyKIsEGLP
S809-p LksPyKIsEGLPtPt
T814-p KIsEGLPtPtKMtPR
T816-p sEGLPtPtKMtPRSR
T819-p LPtPtKMtPRSRILV
S822 PtKMtPRSRILVSIG
S827 PRSRILVSIGESFGT
S831 ILVSIGESFGTSEKF
T834 SIGESFGTSEKFQKI
S835 IGESFGTSEKFQKIN
S848-p INQMVCNsDRVLKRS
K866 GNPPKPLKKLRFDIE
K867 NPPKPLKKLRFDIEG
A875 LRFDIEGADEADGSK
S888 SKHLPAESKFQQKLA
K889 KHLPAESKFQQKLAE
T898 QQKLAEMTSTRTRMQ
S912 QKQRMNESKDVSNKE
K918 ESKDVSNKEEK____
2181 : Phospho-Rb (Ser608) Antibody
3590 : Phospho-Rb (Ser780) (C84F6) Rabbit mAb
8180 : Phospho-Rb (Ser780) (D59B7) Rabbit mAb
9307 : Phospho-Rb (Ser780) Antibody
9301 : Phospho-Rb (Ser795) Antibody
4277 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
8516 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb
8880 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8957 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 555 Conjugate)
8974 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
9308 : Phospho-Rb (Ser807/811) Antibody
11917 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (PE Conjugate)
4277 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
8516 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb
8880 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8957 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 555 Conjugate)
8974 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
9308 : Phospho-Rb (Ser807/811) Antibody
11917 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (PE Conjugate)
  rat

 
A5 ___MPPKAPRRTAAA
S30-p EDDPAQDsDPEELPL
K56 EFIALCQKLKVPDHV
T133 DLLKEIDTSTKVDNA
K136 KEIDTSTKVDNAVSR
S142 TKVDNAVSRLLKKYN
S156 NVLCALYSKLERTCG
S223 LDYFIKLSPPALLRE
Y232 PALLREPYKTAATPI
T234 LLREPYKTAATPING
S242-p AATPINGsPRtPRRG
T245-p PINGsPRtPRRGQNR
K258 NRSARIAKQLESDTR
Y314 LESLSKRYEEVYLKS
Y318 SKRYEEVYLKSKDLD
T338 DHDKTLQTDTIDSFE
D339 HDKTLQTDTIDSFET
T340 DKTLQTDTIDSFETE
S343 LQTDTIDSFETERTP
T346 DTIDSFETERTPRKS
T349 DSFETERTPRKSNPD
S353 TERTPRKSNPDEEAN
T363 DEEANMVTPHtPVRT
T366-p ANMVTPHtPVRTVMN
K420 KDVGHIFKEKFASAV
Y439 IDIGAQRYKLGVRLY
K541 NLTREMIKHLERCEH
S560 SLAWLSDSPLFDLIK
K567 SPLFDLIKQSKDGEG
- gap
S580 EGPDHLESACSLSLP
T593 LPLQSNHTAADMYLs
Y598 NHTAADMYLsPIRSP
S600-p TAADMYLsPIRSPKK
S604 MYLsPIRSPKKRTST
S610 RSPKKRTSTTRVNSA
S616 TSTTRVNSAANTETQ
A617 STTRVNSAANTETQA
K712 KVKNIDLKFKIIVTA
Y763 LKTNILQYASTRPPT
S765 TNILQYASTRPPTLs
T766 NILQYASTRPPTLsP
T770 YASTRPPTLsPIPHI
S772-p STRPPTLsPIPHIPR
S780-p PIPHIPRsPYKFSSs
Y782 PHIPRsPYKFSSsPL
S785 PRsPYKFSSsPLRIP
S786 RsPYKFSSsPLRIPG
S787-p sPYKFSSsPLRIPGG
Y797 RIPGGNIYIsPLKsP
S799-p PGGNIYIsPLKsPYK
K802 NIYIsPLKsPYKISE
K802 NIYIsPLKsPYKISE
K802 NIYIsPLKsPYKISE
S803-p IYIsPLKsPYKISEG
Y805 IsPLKsPYKISEGLP
S808 LKsPYKISEGLPtPt
T813-p KISEGLPtPtKMtPR
T815-p SEGLPtPtKMtPRSR
T818-p LPtPtKMtPRSRILV
S821 PtKMtPRSRILVsIG
S826-p PRSRILVsIGEsFGT
S830-p ILVsIGEsFGTsEKF
T833 sIGEsFGTsEKFQKI
S834-p IGEsFGTsEKFQKIN
S847 INQMVCNSDRVLKRS
K865 GNPPKPLKKLRFDIE
K866 NPPKPLKKLRFDIEG
S874 LRFDIEGSDEADGSK
S887 SKHLPAESKFQQKLA
K888 KHLPAESKFQQKLAE
T897 QQKLAEMTSTRTRMQ
S911 QKQKLNDSMEISNKE
K917 DSMEISNKEEK____
2181 : Phospho-Rb (Ser608) Antibody
3590 : Phospho-Rb (Ser780) (C84F6) Rabbit mAb
8180 : Phospho-Rb (Ser780) (D59B7) Rabbit mAb
9307 : Phospho-Rb (Ser780) Antibody
4277 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
8516 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb
8880 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8957 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 555 Conjugate)
8974 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
9308 : Phospho-Rb (Ser807/811) Antibody
11917 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (PE Conjugate)
4277 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
8516 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb
8880 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8957 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 555 Conjugate)
8974 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
9308 : Phospho-Rb (Ser807/811) Antibody
11917 : Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (PE Conjugate)
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