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Rb (human)

Overview Rb retinoblastoma tumor suppressor protein regulates cell proliferation by controlling progression through the G1-phase restriction point. Has three distinct binding domains and interacts with regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by Cdks inhibits Rb target binding, thus allowing cell cycle progression. Recruits and targets histone methyltransferase suv39h1 leading to epigenetic transcriptional repression. Note: This description may include information from UniProtKB. Protein type: Tumor suppressor; Transcription, coactivator/corepressor; Nuclear receptor co-regulator Cellular Component: nucleoplasm; SWI/SNF complex; Rb-E2F complex; PML body; spindle; chromatin; nucleus Molecular Function: identical protein binding; protein binding; androgen receptor binding; DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; phosphoprotein binding; transcription factor activity; transcription factor binding; kinase binding Biological Process: sister chromatid biorientation; negative regulation of S phase of mitotic cell cycle; positive regulation of transcription, DNA-dependent; G1/S transition checkpoint; regulation of mitotic cell cycle; neuron maturation; cell cycle arrest; G1 phase of mitotic cell cycle; positive regulation of macrophage differentiation; neurite development; negative regulation of epithelial cell proliferation; transcription, DNA-dependent; M phase; negative regulation of transcription factor activity; regulation of lipid kinase activity; enucleate erythrocyte differentiation; chromatin remodeling; G1/S-specific transcription in mitotic cell cycle; myoblast differentiation; neuron apoptosis; maintenance of mitotic sister chromatid cohesion; gut development; cell division; neuron morphogenesis during differentiation; negative regulation of smoothened signaling pathway; virus-host interaction; androgen receptor signaling pathway; G1 phase; Ras protein signal transduction; positive regulation of mitotic metaphase/anaphase transition; striated muscle cell differentiation; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; mitotic cell cycle; negative regulation of transcription, DNA-dependent; S phase of mitotic cell cycle; cell cycle checkpoint; G1/S transition of mitotic cell cycle Reference #:  P06400 (UniProtKB) Alt. Names/Synonyms: OSRC; p105-Rb; pp110; pRb; Rb; RB1; retinoblastoma 1; retinoblastoma suspectibility protein; Retinoblastoma-associated protein Gene Symbols: RB1 Molecular weight: 106,159 Da Basal Isoelectric point: 8.13  Predict pI for various phosphorylation states CST Pathways:  Cell Cycle: G1/S Checkpoint  |  Protein Acetylation Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below Rb 1AD6 - A=378-562 (human) 1GH6 - B=379-772 (human) 1GUX - A=372-589, B=636-787 (human) 1H25 - E=868-878 (human) 1N4M - A/B=380-586 (human) 1O9K - A/C/E/G=372-589, B/D/F/H=636-787 (human) 1PJM - A=860-876 (human) 2AZE - C=829-874 (human) 2QDJ - A=52-355 (human) 2R7G - A/C=643-787 (human) 3N5U - C=870-882 (human) 3POM - A/B=643-783 (human) 4ELJ - A=642-787 (human) 4ELL - A/B=380-787 (human)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1AD6 1GH6 1GUX 1H25 1N4M 1O9K 1PJM 2AZE 2QDJ 2R7G 3N5U 3POM 4ELJ 4ELL  |  Phospho3D  |  DISEASE 109800 180200 259500  |  Source  |  NURSA  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Sites Implicated In
apoptosis, induced: S567‑p, S612‑p apoptosis, inhibited: S780‑p, T821‑p cell cycle regulation: S249‑p, T252‑p, S780‑p, S795‑p, S807‑p, S811‑p, K873‑a, K874‑a cell growth, altered: S780‑p, S788‑p, S795‑p, S807‑p, S811‑p, T821‑p, T826‑p cell growth, induced: S795‑p transcription, altered: S612‑p, S780‑p, S807‑p, S811‑p inhibition: S567‑p, S780‑p, S795‑p intracellular localization: K873‑a, K874‑a molecular association, regulation: T356‑p, T373‑p, S567‑p, S608‑p, S612‑p, S780‑p, S788‑p, S795‑p, S807‑p, S811‑p, T821‑p, T826‑p, K873‑a, K874‑a phosphorylation: K873‑a, K874‑a protein conformation: S608‑p, S612‑p protein degradation: S567‑p, S780‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
1	0		T5-p	___MPPKtPRKTAAT
0	19		S37-p	EEDPEQDsGPEDLPL
0	1		K63	DFTALCQKLKIPDHV
0	1		T140-p	NLLKEIDtSTkVDNA
0	1		K143-u	KEIDtSTkVDNAMsR
0	2		S149-p	TkVDNAMsRLLKKYD
0	1		S163-p	DVLFALFsKLERTCE
2	0		S230-p	LDYFIKLsPPMLLKE
0	1		T241-p	LLKEPYKtAVIPING
13	84		S249-p	AVIPINGsPRtPRRG
14	61		T252-p	PINGsPRtPRRGQNR
0	2		K265-u	NRSARIAkQLENDTR
0	5		Y321-p	VENLSKRyEEIyLKN
0	5		Y325-p	SKRyEEIyLKNKDLD
0	1		T345-p	DHDKTLQtdsIDsFE
0	1		D346-ca	HDKTLQtdsIDsFEt
0	4		S347-p	DKTLQtdsIDsFEtQ
0	7		S350-p	LQtdsIDsFEtQRtP
0	9		T353-p	dsIDsFEtQRtPRKs
7	32		T356-p	DsFEtQRtPRKsNLD
0	1		S360-p	tQRtPRKsNLDEEVN
1	3		P370	DEEVNVIPPHtPVRT
8	48		T373-p	VNVIPPHtPVRTVMN
0	1		K427-a	KDIGYIFkEKFAKAV
0	1		Y446	VEIGSQRYKLGVRLY
0	1		K548-a	NLTREMIkHLERCEH
4	0		S567-p	SLAWLSDsPLFDLIk
0	1		K574-u	sPLFDLIkQSKDREG
0	2		T583-p	SKDREGPtDHLEsAC
0	2		S588-p	GPtDHLEsACPLNLP
0	12		T601-p	LPLQNNHtAADMyLs
0	12		Y606-p	NHtAADMyLsPVRsP
8	33		S608-p	tAADMyLsPVRsPKK
9	38		S612-p	MyLsPVRsPKKKGsT
0	1		S618-p	RsPKKKGsTTRVNst
0	44		S624-p	GsTTRVNstANAETQ
0	5		T625-p	sTTRVNstANAETQA
1	0		K720-s	KVKNIDLkFKIIVTA
0	7		S773-p	TNILQYAstRPPtLs
0	5		T774-p	NILQYAstRPPtLsP
0	60		T778-p	YAstRPPtLsPIPHI
57	115		S780-p	stRPPtLsPIPHIPR
9	71		S788-p	PIPHIPRsPyKFPss
0	25		Y790-p	PHIPRsPyKFPssPL
0	1		P793	PRsPyKFPssPLRIP
0	64		S794-p	RsPyKFPssPLRIPG
34	92		S795-p	sPyKFPssPLRIPGG
1	23		Y805-p	RIPGGNIyIsPLksP
49	126		S807-p	PGGNIyIsPLksPyK
0	2		K810-u	NIyIsPLksPyKIsE
50	88		S811-p	IyIsPLksPyKIsEG
0	12		Y813-p	IsPLksPyKIsEGLP
0	6		S816-p	LksPyKIsEGLPtPt
18	64		T821-p	KIsEGLPtPtKMtPR
0	49		T823-p	sEGLPtPtKMtPRsR
13	64		T826-p	LPtPtKMtPRsRILV
0	1		S829-p	PtKMtPRsRILVsIG
0	2		S834-p	PRsRILVsIGEsFGt
0	2		S838-p	ILVsIGEsFGtsEKF
0	11		T841-p	sIGEsFGtsEKFQKI
0	3		S842-p	IGEsFGtsEKFQKIN
0	13		S855-p	INQMVCNsDRVLKRS
3	0		K873-a	SNPPKPLkkLRFDIE
3	1		K874-a	NPPKPLkkLRFDIEG
0	1		S882-p	LRFDIEGsDEADGSK
0	2		S895-p	SKHLPGEskFQQKLA
0	1		K896-a	KHLPGEskFQQKLAE
0	1		T905-p	QQKLAEMtSTRTRMQ
0	2		S919-p	QKQKMNDsMDTSNkE
0	1		K925-a	DsMDTSNkEEK____

	mouse
A5	___MPPKAPRRAAAA
S31-p	EDDPAQDsGPEELPL
K57-u	EFIALCQkLKVPDHV
T134	DLLKEIDTSTKVDNA
K137	KEIDTSTKVDNAMSR
S143	TKVDNAMSRLLKKYN
S157	NVLCALYSKLERTCE
S224-p	VDYFIKFsPPALLRE
T235	LLREPYKTAAIPING
S243-p	AAIPINGsPRtPRRG
T246-p	PINGsPRtPRRGQNR
K259	NRSARIAKQLENDTR
Y315	VESLSKRYEEVYLKN
Y319	SKRYEEVYLKNKDLD
T339	DHDKTLQTDPIDsFE
D340	HDKTLQTDPIDsFEt
P341	DKTLQTDPIDsFEtE
S344-p	LQTDPIDsFEtERtP
T347-p	DPIDsFEtERtPRKN
T350-p	DsFEtERtPRKNNPD
N354	tERtPRKNNPDEEAN
T364-p	DEEANVVtPHtPVRT
T367-p	ANVVtPHtPVRTVMN
K421	KDVGHIFKEKFANAV
Y440-p	VDIGVQRyKLGVRLY
K542	NLTREMIKHLERCEH
S561-p	SLAWLSDsPLFDLIK
K568	sPLFDLIKQSKDGEG
-	gap
P581	EGPDNLEPACPLSLP
T594-p	LPLQGNHtAADMyLs
Y599-p	NHtAADMyLsPLRsP
S601-p	tAADMyLsPLRsPKK
S605-p	MyLsPLRsPKKRTST
S611	RsPKKRTSTTRVNsA
S617-p	TSTTRVNsAANTETQ
A618	STTRVNsAANTETQA
K713	KVKNIDLKFKIIVTA
S766	TNILQYASTRPPtLs
T767	NILQYASTRPPtLsP
T771-p	YASTRPPtLsPIPHI
S773-p	STRPPtLsPIPHIPR
S781-p	PIPHIPRsPyKFsss
Y783-p	PHIPRsPyKFsssPL
S786-p	PRsPyKFsssPLRIP
S787-p	RsPyKFsssPLRIPG
S788-p	sPyKFsssPLRIPGG
Y798-p	RIPGGNIyIsPLksP
S800-p	PGGNIyIsPLksPyK
K803-u	NIyIsPLksPyKIsE
S804-p	IyIsPLksPyKIsEG
Y806-p	IsPLksPyKIsEGLP
S809-p	LksPyKIsEGLPtPt
T814-p	KIsEGLPtPtKMtPR
T816-p	sEGLPtPtKMtPRSR
T819-p	LPtPtKMtPRSRILV
S822	PtKMtPRSRILVSIG
S827	PRSRILVSIGESFGT
S831	ILVSIGESFGTSEKF
T834	SIGESFGTSEKFQKI
S835	IGESFGTSEKFQKIN
S848-p	INQMVCNsDRVLKRS
K866	GNPPKPLKKLRFDIE
K867	NPPKPLKKLRFDIEG
A875	LRFDIEGADEADGSK
S888	SKHLPAESKFQQKLA
K889	KHLPAESKFQQKLAE
T898	QQKLAEMTSTRTRMQ
S912	QKQRMNESKDVSNKE
K918	ESKDVSNKEEK____

	rat
A5	___MPPKAPRRTAAA
S30	EDDPAQDSDPEELPL
K56	EFIALCQKLKVPDHV
T133	DLLKEIDTSTKVDNA
K136	KEIDTSTKVDNAVSR
S142	TKVDNAVSRLLKKYN
S156	NVLCALYSKLERTCG
S223	LDYFIKLSPPALLRE
T234	LLREPYKTAATPING
S242-p	AATPINGsPRtPRRG
T245-p	PINGsPRtPRRGQNR
K258	NRSARIAKQLESDTR
Y314	LESLSKRYEEVYLKS
Y318	SKRYEEVYLKSKDLD
T338	DHDKTLQTDTIDSFE
D339	HDKTLQTDTIDSFET
T340	DKTLQTDTIDSFETE
S343	LQTDTIDSFETERTP
T346	DTIDSFETERTPRKS
T349	DSFETERTPRKSNPD
S353	TERTPRKSNPDEEAN
T363	DEEANMVTPHtPVRT
T366-p	ANMVTPHtPVRTVMN
K420	KDVGHIFKEKFASAV
Y439	IDIGAQRYKLGVRLY
K541	NLTREMIKHLERCEH
S560	SLAWLSDSPLFDLIK
K567	SPLFDLIKQSKDGEG
-	gap
S580	EGPDHLESACSLSLP
T593	LPLQSNHTAADMYLs
Y598	NHTAADMYLsPIRSP
S600-p	TAADMYLsPIRSPKK
S604	MYLsPIRSPKKRTST
S610	RSPKKRTSTTRVNSA
S616	TSTTRVNSAANTETQ
A617	STTRVNSAANTETQA
K712	KVKNIDLKFKIIVTA
S765	TNILQYASTRPPTLs
T766	NILQYASTRPPTLsP
T770	YASTRPPTLsPIPHI
S772-p	STRPPTLsPIPHIPR
S780-p	PIPHIPRsPYKFSSs
Y782	PHIPRsPYKFSSsPL
S785	PRsPYKFSSsPLRIP
S786	RsPYKFSSsPLRIPG
S787-p	sPYKFSSsPLRIPGG
Y797	RIPGGNIYIsPLKsP
S799-p	PGGNIYIsPLKsPYK
K802	NIYIsPLKsPYKISE
S803-p	IYIsPLKsPYKISEG
Y805	IsPLKsPYKISEGLP
S808	LKsPYKISEGLPtPT
T813-p	KISEGLPtPTKMtPR
T815	SEGLPtPTKMtPRSR
T818-p	LPtPTKMtPRSRILV
S821	PTKMtPRSRILVsIG
S826-p	PRSRILVsIGEsFGT
S830-p	ILVsIGEsFGTsEKF
T833	sIGEsFGTsEKFQKI
S834-p	IGEsFGTsEKFQKIN
S847	INQMVCNSDRVLKRS
K865	GNPPKPLKKLRFDIE
K866	NPPKPLKKLRFDIEG
S874	LRFDIEGSDEADGSK
S887	SKHLPAESKFQQKLA
K888	KHLPAESKFQQKLAE
T897	QQKLAEMTSTRTRMQ
S911	QKQKLNDSMEISNKE
K917	DSMEISNKEEK____

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