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Protein Page:
Btk (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Btk a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; TK group; Tec family
Cellular Component: mast cell granule; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; cytosol; lipid raft
Molecular Function: identical protein binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; protein-tyrosine kinase activity; metal ion binding; non-membrane spanning protein tyrosine kinase activity; ATP binding
Biological Process: I-kappaB kinase/NF-kappaB cascade; adaptive immune response; peptidyl-tyrosine phosphorylation; B cell activation; transcription, DNA-dependent; protein amino acid autophosphorylation; cell maturation; calcium-mediated signaling; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; MyD88-dependent toll-like receptor signaling pathway; regulation of B cell apoptosis; regulation of B cell cytokine production; response to organic substance; response to reactive oxygen species; B cell receptor signaling pathway; histamine secretion by mast cell; toll-like receptor signaling pathway; innate immune response; positive regulation of B cell differentiation; mesoderm development; toll-like receptor 4 signaling pathway
Reference #:  Q06187 (UniProtKB)
Alt. Names/Synonyms: Agammaglobulinaemia tyrosine kinase; AGMX1; AT; ATK; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; BTK; dominant-negative kinase-deficient Brutons tyrosine kinase; IMD1; MGC126261; MGC126262; PSCTK1; Tyrosine-protein kinase BTK; XLA
Gene Symbols: BTK
Molecular weight: 76,281 Da
Basal Isoelectric point: 7.83  Predict pI for various phosphorylation states
CST Pathways:  B Cell Receptor Signaling  |  Tyrosine Kinases & Substrates
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Btk

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cell growth, altered: Y223‑p
activity, induced: Y551‑p
enzymatic activity, induced: Y223‑p, Y551‑p
intracellular localization: Y551‑p
molecular association, regulation: Y551‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 3 S21-p SQQKKKTsPLNFKKR
0 26 Y40-p TVHKLSYyEYDFERG
1 0 S51-p FERGRRGsKKGsIDV
0 1 S55-p RRGsKKGsIDVEKIT
0 1 S93-p SSEMEQIsIIERFPY
1 0 S115 EGPLYVFSPTEELRK
0 1 S174 ILENRNGSLKPGssH
0 2 S179-p NGSLKPGssHRktKK
1 3 S180-p GSLKPGssHRktKKP
0 1 K183-ac KPGssHRktKKPLPP
0 1 T184-p PGssHRktKKPLPPt
0 3 T191-p tKKPLPPtPEEDQIL
16 252 Y223-p LKKVVALyDyMPMNA
0 88 Y225-p KVVALyDyMPMNAND
0 1 Y263-p DKNGQEGyIPSNYVT
0 1 Y279-p AEDSIEMyEWYSKHM
0 1 K300-ub QLLKQEGkEGGFIVR
0 1 S318-p KAGKYTVsVFAkSTG
0 6 K322-ub YTVsVFAkSTGDPQG
0 7 Y334-p PQGVIRHyVVCSTPQ
0 1 S342-p VVCSTPQsQyyLAEK
0 186 Y344-p CSTPQsQyyLAEKHL
0 6 Y345-p STPQsQyyLAEKHLF
0 107 Y361-p TIPELINyHQHNsAG
0 3 S366-p INyHQHNsAGLIsRL
0 1 S371-p HNsAGLIsRLKyPVS
1 22 Y375-p GLIsRLKyPVSQQNK
0 13 K406-ub PKDLTFLkELGTGQF
0 1 Y425 YGKWRGQYDVAIKMI
0 1 S436 IKMIKEGSMSEDEFI
0 15 Y461-p HEKLVQLyGVCTkQR
0 3 K466-ub QLyGVCTkQRPIFII
1 0 T495-p EMRHRFQtQQLLEMC
0 1 Y511-p DVCEAMEyLESKQFL
0 5 K536-ub VNDQGVVkVSDFGLs
0 2 S543-p kVSDFGLsRYVLDDE
22 144 Y551-p RYVLDDEytsSVGSk
0 7 T552-p YVLDDEytsSVGSkF
0 4 S553-p VLDDEytsSVGSkFP
0 12 K558-ub ytsSVGSkFPVRWsP
0 1 S564-p SkFPVRWsPPEVLMY
0 1 T602-p KMPYERFtNsETAEH
0 2 S604-p PYERFtNsETAEHIA
1 1 Y617 IAQGLRLYRPHLASE
0 1 S623 LYRPHLASEKVYTIM
0 1 S659-p LDVMDEEs_______
3537 : Phospho-Btk (Ser180) (3D3) Mouse mAb
5082 : Phospho-Btk (Tyr223) Antibody
  mouse

 
S21-p SQQKKKTsPLNFKKR
Y40-p TVHKLSYyEYDFERG
S51 FERGRRGSKKGSIDV
S55 RRGSKKGSIDVEKIT
S93 SSEMEQISIIERFPY
S115-p EGPLYVFsPTEELRK
S174-p ILENRNGsLKPGssH
S179-p NGsLKPGssHRKTKK
S180-p GsLKPGssHRKTKKP
K183 KPGssHRKTKKPLPP
T184 PGssHRKTKKPLPPt
T191-p TKKPLPPtPEEDQIL
Y223-p LKKVVALyDyMPMNA
Y225-p KVVALyDyMPMNAND
Y263 DKNGQEGYIPSNYIT
Y279 AEDSIEMYEWYSKHM
K300 QLLKQEGKEGGFIVR
S318 KAGKYTVSVFAKSTG
K322 YTVSVFAKSTGEPQG
Y334-p PQGVIRHyVVCSTPQ
S342 VVCSTPQSQyyLAEK
Y344-p CSTPQSQyyLAEKHL
Y345-p STPQSQyyLAEKHLF
Y361-p TIPELINyHQHNSAG
S366 INyHQHNSAGLISRL
S371 HNSAGLISRLKyPVS
Y375-p GLISRLKyPVSKQNK
K406 PKDLTFLKELGTGQF
Y425-p YGKWRGQyDVAIKMI
S436-p IKMIREGsMSEDEFI
Y461-p HEKLVQLyGVCTKQR
K466 QLyGVCTKQRPIFII
T495 EMRHRFQTQQLLEMC
Y511 DVCEAMEYLESKQFL
K536 VNDQGVVKVSDFGLs
S543-p KVSDFGLsRYVLDDE
Y551-p RYVLDDEyTsSVGSK
T552 YVLDDEyTsSVGSKF
S553-p VLDDEyTsSVGSKFP
K558 yTsSVGSKFPVRWSP
S564 SKFPVRWSPPEVLMY
T602 KMPYERFTNSETAEH
S604 PYERFTNSETAEHIA
Y617-p IAQGLRLyRPHLAsE
S623-p LyRPHLAsERVYTIM
S659 LDVMDEES_______
5082 : Phospho-Btk (Tyr223) Antibody
  rat

 
S21 SQQKKKTSPLNFKKR
Y40 TVHKLSYYEYDFERG
S51 FERGRRGSKKGSIDV
S55 RRGSKKGSIDVEKIT
S94 SSEMEQISIIERFPY
S116 EGPLYVFSPTEELRK
S175 ILENRNGSLKPGSSH
S180 NGSLKPGSSHRKTKK
S181 GSLKPGSSHRKTKKP
K184 KPGSSHRKTKKPLPP
T185 PGSSHRKTKKPLPPT
T192 TKKPLPPTPEEDQIL
Y224 LKKVVALYDYMPMNA
Y226 KVVALYDYMPMNAND
Y264 DKNGQEGYIPSNYVT
Y280 AEDSIEMYEWYSKHM
K301 QLLKQEGKEGGFIVR
S319 KAGKYTVSVFAKSTG
K323 YTVSVFAKSTGEPQG
Y335 PQGVIRHYVVCSTPQ
S343 VVCSTPQSQYYLAEK
Y345 CSTPQSQYYLAEKHL
Y346 STPQSQYYLAEKHLF
Y362 TIPELINYHQHNSAG
S367 INYHQHNSAGLISRL
S372 HNSAGLISRLKYPVS
Y376 GLISRLKYPVSKQNK
K407 PKDLTFLKELGTGQF
Y426 YGKWRGQYDVAIKMI
S437 IKMIREGSMSEDEFI
Y462-p HEKLVQLyGVCTKQR
K467 QLyGVCTKQRPIFII
T496 EMRHRFQTQQLLEMC
Y512 DVCEAMEYLESKQFL
K537 VNDQGVVKVSDFGLS
S544 KVSDFGLSRYVLDDE
Y552 RYVLDDEYTSSVGSK
T553 YVLDDEYTSSVGSKF
S554 VLDDEYTSSVGSKFP
K559 YTSSVGSKFPVRWSP
S565 SKFPVRWSPPEVLMY
T603 KIPYERFTNSETAEH
S605 PYERFTNSETAEHIA
Y618 IAQGLRLYRPHLASD
S624 LYRPHLASDRVYTIM
S660 LDVMDEES_______
5082 : Phospho-Btk (Tyr223) Antibody
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