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Protein Page:
Btk (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
Btk a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Tec family
Cellular Component: mast cell granule; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytoplasmic vesicle; cytosol; nucleus; lipid raft
Molecular Function: identical protein binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding
Biological Process: I-kappaB kinase/NF-kappaB cascade; adaptive immune response; transcription, DNA-dependent; B cell activation; protein amino acid autophosphorylation; cell maturation; calcium-mediated signaling; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; response to organic substance; regulation of B cell apoptosis; regulation of B cell cytokine production; MyD88-dependent toll-like receptor signaling pathway; B cell receptor signaling pathway; response to reactive oxygen species; histamine secretion by mast cell; toll-like receptor signaling pathway; positive regulation of B cell differentiation; innate immune response; mesoderm development; toll-like receptor 4 signaling pathway
Reference #:  Q06187 (UniProtKB)
Alt. Names/Synonyms: Agammaglobulinaemia tyrosine kinase; AGMX1; AT; ATK; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; BTK; dominant-negative kinase-deficient Brutons tyrosine kinase; IMD1; MGC126261; MGC126262; PSCTK1; Tyrosine-protein kinase BTK; XLA
Gene Symbols: BTK
Molecular weight: 76,281 Da
Basal Isoelectric point: 7.83  Predict pI for various phosphorylation states
CST Pathways:  B Cell Receptor Signaling  |  Tyrosine Kinases & Substrates
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Btk

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cell growth, altered: Y223‑p
activity, induced: Y551‑p
enzymatic activity, induced: Y223‑p, Y551‑p
intracellular localization: Y551‑p
molecular association, regulation: Y551‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 3 S21-p SQQKKKTsPLNFKKR
0 26 Y40-p TVHKLSYyEYDFERG
1 0 S51-p FERGRRGsKKGsIDV
0 1 S55-p RRGsKKGsIDVEKIT
1 0 S115 EGPLYVFSPTEELRK
0 1 S174 ILENRNGSLKPGssH
0 2 S179-p NGSLKPGssHRKtKK
1 3 S180-p GSLKPGssHRKtKKP
0 1 T184-p PGssHRKtKKPLPPt
0 2 T191-p tKKPLPPtPEEDQIL
15 260 Y223-p LKKVVALyDyMPMNA
0 93 Y225-p KVVALyDyMPMNAND
0 6 K322-u YTVSVFAkSTGDPQG
0 7 Y334-p PQGVIRHyVVCSTPQ
0 1 S342-p VVCSTPQsQyyLAEK
0 190 Y344-p CSTPQsQyyLAEKHL
0 6 Y345-p STPQsQyyLAEKHLF
0 106 Y361-p TIPELINyHQHNsAG
0 3 S366-p INyHQHNsAGLISRL
1 22 Y375-p GLISRLKyPVSQQNK
0 13 K406-u PKDLTFLkELGTGQF
0 15 Y461-p HEKLVQLyGVCTkQR
0 3 K466-u QLyGVCTkQRPIFII
1 0 T495-p EMRHRFQtQQLLEMC
0 1 Y511-p DVCEAMEyLESKQFL
0 5 K536-u VNDQGVVkVSDFGLs
0 2 S543-p kVSDFGLsRYVLDDE
22 143 Y551-p RYVLDDEytsSVGSk
0 7 T552-p YVLDDEytsSVGSkF
0 5 S553-p VLDDEytsSVGSkFP
0 12 K558-u ytsSVGSkFPVRWSP
0 1 T602-p KMPYERFtNsETAEH
0 2 S604-p PYERFtNsETAEHIA
1 1 Y617 IAQGLRLYRPHLASE
0 1 S623 LYRPHLASEKVYTIM
0 1 S659-p LDVMDEEs_______
3537 : Phospho-Btk (Ser180) (3D3) Mouse mAb
5082 : Phospho-Btk (Tyr223) Antibody
  mouse

 
S21-p SQQKKKTsPLNFKKR
Y40-p TVHKLSYyEYDFERG
S51 FERGRRGSKKGSIDV
S55 RRGSKKGSIDVEKIT
S115-p EGPLYVFsPTEELRK
S174-p ILENRNGsLKPGssH
S179-p NGsLKPGssHRKTKK
S180-p GsLKPGssHRKTKKP
T184 PGssHRKTKKPLPPt
T191-p TKKPLPPtPEEDQIL
Y223-p LKKVVALyDyMPMNA
Y225-p KVVALyDyMPMNAND
K322 YTVSVFAKSTGEPQG
Y334-p PQGVIRHyVVCSTPQ
S342 VVCSTPQSQyyLAEK
Y344-p CSTPQSQyyLAEKHL
Y345-p STPQSQyyLAEKHLF
Y361-p TIPELINyHQHNSAG
S366 INyHQHNSAGLISRL
Y375-p GLISRLKyPVSKQNK
K406 PKDLTFLKELGTGQF
Y461-p HEKLVQLyGVCTKQR
K466 QLyGVCTKQRPIFII
T495 EMRHRFQTQQLLEMC
Y511 DVCEAMEYLESKQFL
K536 VNDQGVVKVSDFGLs
S543-p KVSDFGLsRYVLDDE
Y551-p RYVLDDEyTsSVGSK
T552 YVLDDEyTsSVGSKF
S553-p VLDDEyTsSVGSKFP
K558 yTsSVGSKFPVRWSP
T602 KMPYERFTNSETAEH
S604 PYERFTNSETAEHIA
Y617-p IAQGLRLyRPHLAsE
S623-p LyRPHLAsERVYTIM
S659 LDVMDEES_______
5082 : Phospho-Btk (Tyr223) Antibody
  rat

 
S21 SQQKKKTSPLNFKKR
Y40 TVHKLSYYEYDFERG
S51 FERGRRGSKKGSIDV
S55 RRGSKKGSIDVEKIT
S116 EGPLYVFSPTEELRK
S175 ILENRNGSLKPGSSH
S180 NGSLKPGSSHRKTKK
S181 GSLKPGSSHRKTKKP
T185 PGSSHRKTKKPLPPT
T192 TKKPLPPTPEEDQIL
Y224 LKKVVALYDYMPMNA
Y226 KVVALYDYMPMNAND
K323 YTVSVFAKSTGEPQG
Y335 PQGVIRHYVVCSTPQ
S343 VVCSTPQSQYYLAEK
Y345 CSTPQSQYYLAEKHL
Y346 STPQSQYYLAEKHLF
Y362 TIPELINYHQHNSAG
S367 INYHQHNSAGLISRL
Y376 GLISRLKYPVSKQNK
K407 PKDLTFLKELGTGQF
Y462-p HEKLVQLyGVCTKQR
K467 QLyGVCTKQRPIFII
T496 EMRHRFQTQQLLEMC
Y512 DVCEAMEYLESKQFL
K537 VNDQGVVKVSDFGLS
S544 KVSDFGLSRYVLDDE
Y552 RYVLDDEYTSSVGSK
T553 YVLDDEYTSSVGSKF
S554 VLDDEYTSSVGSKFP
K559 YTSSVGSKFPVRWSP
T603 KIPYERFTNSETAEH
S605 PYERFTNSETAEHIA
Y618 IAQGLRLYRPHLASD
S624 LYRPHLASDRVYTIM
S660 LDVMDEES_______
5082 : Phospho-Btk (Tyr223) Antibody
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