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Protein Page:
SQSTM1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
SQSTM1 an adapter protein which binds ubiquitin, shuttling proteins to the proteasome. Co-localizes along with the E3 ubiquitin ligase, TRAF6, to aggregates from Alzheimer's disease brains but not in control brain. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Forms ternary complexes with PKCZ and Kv-beta2 or PKCZ and GABBR3. Also interacts with KCNAB1, GABRR1, GABRR2 and GABRR3. Interacts with EBI3, LCK, RASA1, PKCZ , PKCI, NR2F2, NTRK1, NTRK2, NTRK3, NBR1, MEK5, TRIM55 and MAPKAPK5. Interacts with the proteasome subunits PSMD4 and PSMC2. Interacts with K63- polyubiquitinated MAPT/TAU. Interacts with IKBKB through PRKCZ and PRKCI. Interacts with NGFR through TRAF6 and bridges that complex to NTRK1. Forms a complex with MEK5 and PRKCZ or PRKCI. Component of a ternary complex with PAWR and PRKCZ. Upon TNF-alpha stimulation, interacts with RIPK1 problably bridging IKBKB to the TNF-R1 complex composed of TNF-R1/TNFRSF1A, TRADD and RIPK1. Forms a complex with JUB/Ajuba, PRKCZ and TRAF6. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; Autophagy; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 5q35
Cellular Component: nucleoplasm; PML body; lysosome; endoplasmic reticulum; late endosome; cytoplasm; autophagic vacuole; cytoplasmic vesicle; inclusion body; cytosol
Molecular Function: protein serine/threonine kinase activity; identical protein binding; ubiquitin binding; protein binding; protein homodimerization activity; protein kinase C binding; zinc ion binding; SH2 domain binding; receptor tyrosine kinase binding; protein kinase binding
Biological Process: ubiquitin-dependent protein catabolic process; regulation of Ras protein signal transduction; nerve growth factor receptor signaling pathway; immune system process; macroautophagy; protein heterooligomerization; positive regulation of apoptosis; endosome transport; protein amino acid phosphorylation; protein localization; response to stress; autophagy; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; cell differentiation; regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of macroautophagy; negative regulation of apoptosis
Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 3; Paget Disease Of Bone
Reference #:  Q13501 (UniProtKB)
Alt. Names/Synonyms: A170; EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; ORCA; OSIL; oxidative stress induced like; p60; p62; p62B; PDB3; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; sequestosome 1; Sequestosome-1; SQSTM; SQSTM1; Ubiquitin-binding protein p62; ZIP3
Gene Symbols: SQSTM1
Molecular weight: 47,687 Da
Basal Isoelectric point: 5.1  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

SQSTM1

Protein Structure Not Found.


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Sites Implicated In
autophagy, induced: S403‑p
carcinogenesis, altered: T269‑p, S272‑p
cell cycle regulation: T269‑p, S272‑p
intracellular localization: S349‑p
protein degradation: T269‑p, S272‑p
ubiquitination: T269‑p, S272‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 2 Y9-p ASLTVKAyLLGkEDA
0 1 K13-ac VKAyLLGkEDAAREI
0 49 K13-ub VKAyLLGkEDAAREI
1 75 S24-p AREIRRFsFCCsPEP
0 12 S28-p RRFsFCCsPEPEAEA
0 2 S49-p GPCERLLsRVAALFP
0 2 Y67-p PGGFQAHyRDEDGDL
0 31 K91-ub TMAMSYVkDDIFRIY
0 35 K141-ub PVVGTRYkCSVCPDy
0 46 Y148-p kCSVCPDyDLCsVCE
0 3 S152-p CPDyDLCsVCEGkGL
0 66 K157-ub LCsVCEGkGLHRGHT
0 28 K165-ub GLHRGHTkLAFPsPF
0 7 S170-p HTkLAFPsPFGHLsE
0 4 S176-p PsPFGHLsEGFSHsR
0 3 G178 PFGHLsEGFSHsRWL
0 3 S182-p LsEGFSHsRWLRkVk
0 5 K187-ub SHsRWLRkVkHGHFG
0 12 K189-ub sRWLRkVkHGHFGWP
1 18 S207-p MGPPGNWsPRPPRAG
0 1 S224-p RPGPTAEsAsGPSED
0 2 S226-p GPTAEsAsGPSEDPs
0 2 S233-p sGPSEDPsVNFLKNV
0 2 S249-p ESVAAALsPLGIEVD
0 24 K264-ub IDVEHGGkRsRLtPV
0 20 S266-p VEHGGkRsRLtPVsP
2 172 T269-p GGkRsRLtPVsPEss
0 6 V271 kRsRLtPVsPEssst
2 314 S272-p RsRLtPVsPEssstE
0 29 S275-p LtPVsPEssstEEks
0 34 S276-p tPVsPEssstEEksS
0 42 S277-p PVsPEssstEEksSs
0 11 T278-p VsPEssstEEksSsQ
0 1 K281-ub EssstEEksSsQPss
1 4 S282-p ssstEEksSsQPssC
0 7 S284-p stEEksSsQPssCCs
0 1 S287-p EksSsQPssCCsDPs
0 4 S288-p ksSsQPssCCsDPsk
0 5 S291-p sQPssCCsDPskPGG
0 2 S294-p ssCCsDPskPGGNVE
0 23 K295-ub sCCsDPskPGGNVEG
0 4 S306-p NVEGATQsLAEQMRk
0 2 R312 QsLAEQMRkIALESE
0 17 K313-ub sLAEQMRkIALESEG
0 1 S318 MRkIALESEGRPEEQ
0 15 S328-p RPEEQMEsDNCsGGD
1 23 S332-p QMEsDNCsGGDDDWt
0 4 T339-p sGGDDDWtHLsskEV
0 4 S342-p DDDWtHLsskEVDPs
0 4 S343-p DDWtHLsskEVDPsT
0 1 K344-ac DWtHLsskEVDPsTG
5 1 S349-p sskEVDPsTGELQsL
0 14 S355-p PsTGELQsLQMPEsE
0 11 S361-p QsLQMPEsEGPssLD
0 5 S365-p MPEsEGPssLDPsQE
1 17 S366-p PEsEGPssLDPsQEG
0 7 S370-p GPssLDPsQEGPtGL
0 3 T375-p DPsQEGPtGLkEAAL
0 2 K378-ac QEGPtGLkEAALyPH
0 3 Y383-p GLkEAALyPHLPPEA
5 2 S403-p ESLSQMLsMGFSDEG
1 0 S407 QMLsMGFSDEGGWLT
0 26 K420-ub LTRLLQTkNYDIGAA
0 1 T430-p DIGAALDtIQyskHP
0 3 Y433-p AALDtIQyskHPPPL
0 2 S434-p ALDtIQyskHPPPL_
0 98 K435-ub LDtIQyskHPPPL__
13121 : Phospho-SQSTM1/p62 (Thr269/Ser272)
13121 : Phospho-SQSTM1/p62 (Thr269/Ser272)
95697 : Phospho-SQSTM1/p62 (Ser349) Antibody
14354 : Phospho-SQSTM1/p62 (Ser403) Antibody
  SQSTM1 iso2  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
K7 _MAMSYVKDDIFRIY
K57 PVVGTRYKCSVCPDY
Y64 KCSVCPDYDLCSVCE
S68 CPDYDLCSVCEGKGL
K73 LCSVCEGKGLHRGHT
K81 GLHRGHTKLAFPSPF
S86 HTKLAFPSPFGHLSE
S92 PSPFGHLSEGFSHSR
G94 PFGHLSEGFSHSRWL
S98 LSEGFSHSRWLRKVK
K103 SHSRWLRKVKHGHFG
K105 SRWLRKVKHGHFGWP
S123 MGPPGNWSPRPPRAG
S140 RPGPTAESASGPSED
S142 GPTAESASGPSEDPS
S149 SGPSEDPSVNFLKNV
S165 ESVAAALSPLGIEVD
K180 IDVEHGGKRSRLTPV
S182 VEHGGKRSRLTPVSP
T185 GGKRSRLTPVSPESS
V187 KRSRLTPVSPESSST
S188 RSRLTPVSPESSSTE
S191 LTPVSPESSSTEEKS
S192 TPVSPESSSTEEKSS
S193 PVSPESSSTEEKSSS
T194 VSPESSSTEEKSSSQ
K197 ESSSTEEKSSSQPSS
S198 SSSTEEKSSSQPSSC
S200 STEEKSSSQPSSCCS
S203 EKSSSQPSSCCSDPS
S204 KSSSQPSSCCSDPSK
S207 SQPSSCCSDPSKPGG
S210 SSCCSDPSKPGGNVE
K211 SCCSDPSKPGGNVEG
S222 NVEGATQSLAEQMRK
R228 QSLAEQMRKIALESE
K229 SLAEQMRKIALESEG
S234 MRKIALESEGRPEEQ
S244 RPEEQMESDNCSGGD
S248 QMESDNCSGGDDDWT
T255 SGGDDDWTHLSSKEV
S258 DDDWTHLSSKEVDPS
S259 DDWTHLSSKEVDPST
K260 DWTHLSSKEVDPSTG
S265 SSKEVDPSTGELQSL
S271 PSTGELQSLQMPESE
S277 QSLQMPESEGPSSLD
S281 MPESEGPSSLDPSQE
S282 PESEGPSSLDPSQEG
S286 GPSSLDPSQEGPTGL
T291 DPSQEGPTGLKEAAL
K294 QEGPTGLKEAALYPH
Y299 GLKEAALYPHLPPEA
S319 ESLSQMLSMGFSDEG
S323 QMLSMGFSDEGGWLT
K336 LTRLLQTKNYDIGAA
T346 DIGAALDTIQYSKHP
Y349 AALDTIQYSKHPPPL
S350 ALDTIQYSKHPPPL_
K351 LDTIQYSKHPPPL__
95697 : Phospho-SQSTM1/p62 (Ser349) Antibody
  mouse

 
Y9 ASFTVKAYLLGkEEA
K13 VKAYLLGKEEATREI
K13-ub VKAYLLGkEEATREI
S24-p TREIRRFsFCFsPEP
S28-p RRFsFCFsPEPEAEA
S49 GPCERLLSRVAVLFP
Y67 PGGFQAHYRDEDGDL
K91-ub TMAMSYVkDDIFRIY
K141-ub PVVGTRYkCSVCPDy
Y148-p kCSVCPDyDLCsVCE
S152-p CPDyDLCsVCEGkGL
K157-ub LCsVCEGkGLHREHS
K165-ub GLHREHSkLIFPNPF
N170 HSkLIFPNPFGHLSD
S176 PNPFGHLSDsFSHSR
S178-p PFGHLSDsFSHSRWL
S182 LSDsFSHSRWLRKLK
K187 SHSRWLRKLKHGHFG
K189 SRWLRKLKHGHFGWP
S207-p MGPPGNWsPRPPRAG
S224 RPCPTAESASAPPED
S226 CPTAESASAPPEDPN
N233 SAPPEDPNVNFLKNV
S249 ESVAAALSPLGIEVD
K264 IDVEHGGKRsRLtPt
S266-p VEHGGKRsRLtPttP
T269-p GGKRsRLtPttPEss
T271-p KRsRLtPttPEssst
T272-p RsRLtPttPEssstG
S275-p LtPttPEssstGTED
S276-p tPttPEssstGTEDK
S277-p PttPEssstGTEDKS
T278-p ttPEssstGTEDKSN
K283 sstGTEDKSNtQPSS
S284 stGTEDKSNtQPSSC
T286-p GTEDKSNtQPSSCSS
S289 DKSNtQPSSCSSEVs
S290 KSNtQPSSCSSEVsk
S293 tQPSSCSSEVskPDG
S296-p SSCSSEVskPDGAGE
K297-ub SCSSEVskPDGAGEG
S308-p AGEGPAQsLTEQMkk
K314-ub QsLTEQMkkIALEsV
K315-ub sLTEQMkkIALEsVG
S320-p MkkIALEsVGQPEEQ
S330-p QPEEQMEsGNCsGGD
S334-p QMEsGNCsGGDDDWt
T341-p sGGDDDWtHLSsKEV
S344 DDDWtHLSsKEVDPs
S345-p DDWtHLSsKEVDPsT
K346 DWtHLSsKEVDPsTG
S351-p SsKEVDPsTGELQsL
S357-p PsTGELQsLQMPEsE
S363-p QsLQMPEsEGPssLD
S367-p MPEsEGPssLDPsQE
S368-p PEsEGPssLDPsQEG
S372-p GPssLDPsQEGPTGL
T377 DPsQEGPTGLKEAAL
K380 QEGPTGLKEAALYPH
Y385 GLKEAALYPHLPPEA
S405-p ESLSQMLsMGFsDEG
S409-p QMLsMGFsDEGGWLT
K422-ub LTRLLQTkNYDIGAA
T432 DIGAALDTIQYSkHP
Y435 AALDTIQYSkHPPPL
S436 ALDTIQYSkHPPPL_
K437-ub LDTIQYSkHPPPL__
13121 : Phospho-SQSTM1/p62 (Thr269/Ser272)
13121 : Phospho-SQSTM1/p62 (Thr269/Ser272)
95697 : Phospho-SQSTM1/p62 (Ser349) Antibody
14354 : Phospho-SQSTM1/p62 (Ser403) Antibody
  rat

 
Y9 ASLTVKAYLLGKEEA
K13 VKAYLLGKEEAAREI
K13 VKAYLLGKEEAAREI
S24-p AREIRRFsFCFSPEP
S28 RRFsFCFSPEPEAEA
S47 GPCERLLSRVAVLFP
Y65 PGGFQAHYRDEDGDL
K89 TMAMSYVKDDIFRIY
K138 PVVGTRYKCSVCPDY
Y145 KCSVCPDYDLCSVCE
S149 CPDYDLCSVCEGKGL
K154 LCSVCEGKGLHREHS
K162 GLHREHSKLIFPNPF
N167 HSKLIFPNPFGHLSD
S173 PNPFGHLSDSFSHSR
S175 PFGHLSDSFSHSRWL
S179 LSDSFSHSRWLRKLK
K184 SHSRWLRKLKHGHFG
K186 SRWLRKLKHGHFGWP
S204 MGPPGNWSPRPPRAG
S221 RPCPTAESASAPSED
S223 CPTAESASAPSEDPN
N230 SAPSEDPNVNFLKNV
S246 ESVAAALSPLGIEVD
K261 IDVEHGGKRSRLtPT
S263 VEHGGKRSRLtPTSA
T266-p GGKRSRLtPTSAESS
T268 KRSRLtPTSAESSST
S269 RSRLtPTSAESSSTG
S272 LtPTSAESSSTGTED
S273 tPTSAESSSTGTEDK
S274 PTSAESSSTGTEDKS
T275 TSAESSSTGTEDKSG
K280 SSTGTEDKSGTQPSS
S281 STGTEDKSGTQPSSC
T283 GTEDKSGTQPSSCSS
S286 DKSGTQPSSCSSEVS
S287 KSGTQPSSCSSEVSK
S290 TQPSSCSSEVSKPDG
S293 SSCSSEVSKPDGAGE
K294 SCSSEVSKPDGAGEG
S305 AGEGPAQSLTEQMKK
K311 QSLTEQMKKIALESV
K312 SLTEQMKKIALESVG
S317 MKKIALESVGQPEEL
S327 QPEELMESDNCSGGD
S331 LMESDNCSGGDDDWT
T338 SGGDDDWTHLSSKEV
S341 DDDWTHLSSKEVDPS
S342 DDWTHLSSKEVDPST
K343 DWTHLSSKEVDPSTG
S348 SSKEVDPSTGELQsL
S354-p PSTGELQsLQMPEsE
S360-p QsLQMPEsEGPSsLD
S364 MPEsEGPSsLDPSQE
S365-p PEsEGPSsLDPSQEG
S369 GPSsLDPSQEGPTGL
T374 DPSQEGPTGLKEAAL
K377 QEGPTGLKEAALYPH
Y382 GLKEAALYPHLPPEA
S402 ESLSQMLSMGFSDEG
S406 QMLSMGFSDEGGWLT
K419 LTRLLQTKNYDIGAA
T429 DIGAALDTIQYSKHP
Y432 AALDTIQYSKHPPPL
S433 ALDTIQYSKHPPPL_
K434 LDTIQYSKHPPPL__
13121 : Phospho-SQSTM1/p62 (Thr269/Ser272)
13121 : Phospho-SQSTM1/p62 (Thr269/Ser272)
95697 : Phospho-SQSTM1/p62 (Ser349) Antibody
14354 : Phospho-SQSTM1/p62 (Ser403) Antibody
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