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Protein Page:
SF2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
SF2 a serine-arginine-rich splicing regulatory protein. Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5' and 3' splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice-site-containing pre-mRNA. Extensively phosphorylated on serine residues in the serine-arginine rich region. Three splice variant isoforms have been described. ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. Note: This description may include information from UniProtKB.
Protein type: Spliceosome; RNA binding protein; RNA splicing
Chromosomal Location of Human Ortholog: 17q22
Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus
Molecular Function: mRNA binding; protein binding; RS domain binding; RNA binding; nucleotide binding
Biological Process: nuclear mRNA 5'-splice site recognition; transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA splice site selection; mRNA export from nucleus; in utero embryonic development; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription; cardiac muscle contraction
Reference #:  Q07955 (UniProtKB)
Alt. Names/Synonyms: Alternative-splicing factor 1; ASF; ASF-1; MGC5228; pre-mRNA-splicing factor SF2, P33 subunit; SF2; SF2P33; SFRS1; splicing factor 2; Splicing factor, arginine/serine-rich 1; SRp30a
Gene Symbols: SRSF1
Molecular weight: 27,745 Da
Basal Isoelectric point: 10.37  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

SF2

Protein Structure Not Found.


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Sites Implicated In
RNA splicing, inhibited: S119‑p
activity, inhibited: S119‑p
molecular association, regulation: S119‑p
protein conformation: S207‑p, S209‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 10 S2-p ______MsGGGVIRG
0 2 Y19-p GNNDCRIyVGNLPPD
0 7 K30-ub LPPDIRTkDIEDVFy
0 8 Y37-p kDIEDVFykYGAIRD
0 4 K38-ac DIEDVFykYGAIRDI
0 59 K38-ub DIEDVFykYGAIRDI
0 1 Y39 IEDVFykYGAIRDID
0 1 R51-m1 DIDLKNRrGGPPFAF
0 1 Y72-p RDAEDAVyGRDGyDY
0 6 Y77-p AVyGRDGyDYDGYRL
1 13 R93-m1 VEFPRSGrGTGrGGG
0 3 R93-m2 VEFPRSGrGTGrGGG
1 18 R97-m1 RSGrGTGrGGGGGGG
0 4 R97-m2 RSGrGTGrGGGGGGG
1 17 R109-m1 GGGGGAPrGryGPPs
0 3 R109-m2 GGGGGAPrGryGPPs
0 4 R111-m1 GGGAPrGryGPPsRR
0 1 Y112-p GGAPrGryGPPsRRs
0 1 S116-p rGryGPPsRRsENRV
1 1 S119-p yGPPsRRsENRVVVS
0 1 S126 sENRVVVSGLPPSGs
0 4 S133-p SGLPPSGsWQDLkDH
0 2 K138-ac SGsWQDLkDHMREAG
0 2 K138-ub SGsWQDLkDHMREAG
0 8 Y149-p REAGDVCyADVyRDG
0 1 Y153-p DVCyADVyRDGtGVV
0 1 T157-p ADVyRDGtGVVEFVR
0 1 T169-p FVRKEDMtyAVRkLD
0 16 Y170-p VRKEDMtyAVRkLDN
0 2 K174-ub DMtyAVRkLDNTkFR
0 3 K179-ac VRkLDNTkFRSHEGE
0 2 K179-ub VRkLDNTkFRSHEGE
0 7 T187-p FRSHEGEtAyIRVKV
0 389 Y189-p SHEGEtAyIRVKVDG
1 110 S199-p VKVDGPRsPsyGRsR
1 49 S201-p VDGPRsPsyGRsRsR
0 10 Y202-p DGPRsPsyGRsRsRs
1 47 S205-p RsPsyGRsRsRsRsR
2 1 S207-p PsyGRsRsRsRsRsR
2 0 S209-p yGRsRsRsRsRsRsR
1 0 S211-p RsRsRsRsRsRsRsR
1 0 S213-p RsRsRsRsRsRsRsN
1 0 S215-p RsRsRsRsRsRsNsR
1 0 S217-p RsRsRsRsRsNsRsR
1 0 S219-p RsRsRsRsNsRsRsy
1 12 S221-p RsRsRsNsRsRsysP
1 15 S223-p RsRsNsRsRsysPRR
1 11 S225-p RsNsRsRsysPRRsR
0 4 Y226-p sNsRsRsysPRRsRG
3 15 S227-p NsRsRsysPRRsRGs
0 16 S231-p RsysPRRsRGsPRys
2 38 S234-p sPRRsRGsPRysPRH
0 11 Y237-p RsRGsPRysPRHsRs
1 41 S238-p sRGsPRysPRHsRsR
0 11 S242-p PRysPRHsRsRsRt_
0 3 S244-p ysPRHsRsRsRt___
0 1 S246-p PRHsRsRsRt_____
0 1 T248-p HsRsRsRt_______
  mouse

 
S2-p ______MsGGGVIRG
Y19 GNNDCRIYVGNLPPD
K30-ub LPPDIRTkDIEDVFY
Y37 kDIEDVFYkYGAIRD
K38-ac DIEDVFYkYGAIRDI
K38-ub DIEDVFYkYGAIRDI
Y39 IEDVFYkYGAIRDID
R51-m1 DIDLKNRrGGPPFAF
Y72 RDAEDAVYGRDGYDY
Y77 AVYGRDGYDYDGYRL
R93-m1 VEFPRSGrGTGrGGG
R93 VEFPRSGRGTGrGGG
R97-m1 RSGrGTGrGGGGGGG
R97-m2 RSGrGTGrGGGGGGG
R109-m1 GGGGGAPrGrYGPPS
R109-m2 GGGGGAPrGrYGPPS
R111-m1 GGGAPrGrYGPPSRR
Y112 GGAPrGrYGPPSRRS
S116 rGrYGPPSRRSENRV
S119 YGPPSRRSENRVVVs
S126-p SENRVVVsGLPPSGs
S133-p sGLPPSGsWQDLKDH
K138 SGsWQDLKDHMREAG
K138 SGsWQDLKDHMREAG
Y149 REAGDVCYADVYRDG
Y153 DVCYADVYRDGTGVV
T157 ADVYRDGTGVVEFVR
T169 FVRKEDMTyAVRkLD
Y170-p VRKEDMTyAVRkLDN
K174-ub DMTyAVRkLDNTkFR
K179 VRkLDNTKFRSHEGE
K179-ub VRkLDNTkFRSHEGE
T187-p FRSHEGEtAyIRVKV
Y189-p SHEGEtAyIRVKVDG
S199-p VKVDGPRsPsyGRsR
S201-p VDGPRsPsyGRsRsR
Y202-p DGPRsPsyGRsRsRS
S205-p RsPsyGRsRsRSRSR
S207-p PsyGRsRsRSRSRSR
S209 yGRsRsRSRSRSRSR
S211 RsRsRSRSRSRSRSR
S213 RsRSRSRSRSRSRSN
S215 RSRSRSRSRSRSNsR
S217 RSRSRSRSRSNsRsR
S219 RSRSRSRSNsRsRsy
S221-p RSRSRSNsRsRsysP
S223-p RSRSNsRsRsysPRR
S225-p RSNsRsRsysPRRsR
Y226-p SNsRsRsysPRRsRG
S227-p NsRsRsysPRRsRGs
S231-p RsysPRRsRGsPRys
S234-p sPRRsRGsPRysPRH
Y237-p RsRGsPRysPRHsRS
S238-p sRGsPRysPRHsRSR
S242-p PRysPRHsRSRSRT_
S244 ysPRHsRSRSRT___
S246 PRHsRSRSRT_____
T248 HsRSRSRT_______
  rat

 
S2 ______MSGGGVIRG
Y19 GNNDCRIYVGNLPPD
K30 LPPDIRTKDIEDVFY
Y37 KDIEDVFYkyGAIRD
K38-ac DIEDVFYkyGAIRDI
K38 DIEDVFYKyGAIRDI
Y39-p IEDVFYkyGAIRDID
R51 DIDLKNRRGGPPFAF
Y72 RDAEDAVYGRDGYDY
Y77 AVYGRDGYDYDGYRL
R93 VEFPRSGRGTGRGGG
R93 VEFPRSGRGTGRGGG
R97 RSGRGTGRGGGGGGG
R97 RSGRGTGRGGGGGGG
R109 GGGGGAPRGRYGPPS
R109 GGGGGAPRGRYGPPS
R111 GGGAPRGRYGPPSRR
Y112 GGAPRGRYGPPSRRS
S116 RGRYGPPSRRSENRV
S119 YGPPSRRSENRVVVS
S126 SENRVVVSGLPPSGS
S133 SGLPPSGSWQDLKDH
K138 SGSWQDLKDHMREAG
K138 SGSWQDLKDHMREAG
Y149 REAGDVCYADVYRDG
Y153 DVCYADVYRDGTGVV
T157 ADVYRDGTGVVEFVR
T169 FVRKEDMTYAVRKLD
Y170 VRKEDMTYAVRKLDN
K174 DMTYAVRKLDNTkFR
K179-ac VRKLDNTkFRSHEGE
K179 VRKLDNTKFRSHEGE
T187-p FRSHEGEtAyIRVKV
Y189-p SHEGEtAyIRVKVDG
S199-p VKVDGPRsPsyGRsR
S201-p VDGPRsPsyGRsRSR
Y202-p DGPRsPsyGRsRSRS
S205-p RsPsyGRsRSRSRSR
S207 PsyGRsRSRSRSRSR
S209 yGRsRSRSRSRSRSR
S211 RsRSRSRSRSRSRSR
S213 RSRSRSRSRSRSRSN
S215 RSRSRSRSRSRSNSR
S217 RSRSRSRSRSNSRsR
S219 RSRSRSRSNSRsRsY
S221 RSRSRSNSRsRsYsP
S223-p RSRSNSRsRsYsPRR
S225-p RSNSRsRsYsPRRsR
Y226 SNSRsRsYsPRRsRG
S227-p NSRsRsYsPRRsRGs
S231-p RsYsPRRsRGsPRYs
S234-p sPRRsRGsPRYsPRH
Y237 RsRGsPRYsPRHsRs
S238-p sRGsPRYsPRHsRsR
S242-p PRYsPRHsRsRSRT_
S244-p YsPRHsRsRSRT___
S246 PRHsRsRSRT_____
T248 HsRsRSRT_______
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