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Protein Page:
PFKL (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PFKL phosphofructokinase, liver type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Two alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Carbohydrate Metabolism - galactose; EC 2.7.1.11; Kinase, other; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose
Cellular Component: 6-phosphofructokinase complex; membrane; cytosol
Molecular Function: identical protein binding; protein binding; metal ion binding; kinase binding; ATP binding; 6-phosphofructokinase activity
Biological Process: fructose 1,6-bisphosphate metabolic process; glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; response to glucose stimulus; glucose metabolic process; fructose 6-phosphate metabolic process; protein oligomerization; protein homotetramerization; negative regulation of insulin secretion
Reference #:  P17858 (UniProtKB)
Alt. Names/Synonyms: 6-phosphofructokinase, liver type; DKFZp686G1648; DKFZp686L2097; FLJ30173; FLJ40909; K6PL; liver phosphofructokinase; liver-type 1-phosphofructokinase; PFK-B; PFKL; Phosphofructo-1-kinase isozyme B; Phosphofructokinase 1; phosphofructokinase, liver; Phosphohexokinase
Gene Symbols: PFKL
Molecular weight: 85,018 Da
Basal Isoelectric point: 7.26  Predict pI for various phosphorylation states
CST Pathways:  Warburg Effect
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PFKL

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 K8 MAAVDLEKLRASGAG
0 2 K16-ub LRASGAGkAIGVLTS
0 1 Y43 AVTRMGIYVGAKVFL
0 1 T93-p SARCKAFtTREGRRA
0 1 K144-ub EELVAEGkISETTAR
0 3 T304-p GHVQRGGtPSAFDRI
0 1 S377 ATQLRGGSFENNWNI
0 1 K469-ac GGSMLGTkRTLPkGQ
0 1 K474-ub GTkRTLPkGQLEsIV
0 1 S479-p LPkGQLEsIVENIRI
0 4 K563-ac KQSASGTkRRVFIVE
0 1 K614-ub NVEHMTEkMKTDIQR
0 21 Y633-p RNEKCHDyyTTEFLy
0 12 Y634-p NEKCHDyyTTEFLyN
0 5 Y640-p yyTTEFLyNLYSSEG
0 2 Y674-p PTPFDRNyGTKLGVK
0 1 K689-ac AMLWLSEkLREVYRK
0 4 K726-ac FSPVTELkkDTDFEH
0 1 K727-ac SPVTELkkDTDFEHR
1 4 T773-p LEHVTRRtLsMDKGF
0 45 S775-p HVTRRtLsMDKGF__
  PFKL iso2  
- gap
- gap
Y90-p AVTRMGIyVGAKVFL
T140 SARCKAFTTREGRRA
K191 EELVAEGKISETTAR
T351 GHVQRGGTPSAFDRI
S424 ATQLRGGSFENNWNI
K516 GGSMLGTKRTLPKGQ
K521 GTKRTLPKGQLESIV
S526 LPKGQLESIVENIRI
K610 KQSASGTKRRVFIVE
K661 NVEHMTEKMKTDIQR
Y680-p RNEKCHDyYTTEFLY
Y681 NEKCHDyYTTEFLYN
Y687 yYTTEFLYNLYSSEG
Y721 PTPFDRNYGTKLGVK
K736 AMLWLSEKLREVYRK
K773 FSPVTELKKDTDFEH
K774 SPVTELKKDTDFEHR
T820 LEHVTRRTLSMDKGF
S822 HVTRRTLSMDKGF__
  mouse

 
K8-ub MATVDLEkLRMSGAG
K16 LRMSGAGKAIGVLTS
Y43 AVTRMGIYVGAKVFL
T93 SARCKAFTTREGRLA
K144 EELVKEGKISESTAQ
T304-p GHVQRGGtPSAFDRI
S377-p AIQLRGRsFENNWKI
K469 GGSMLGTKRTLPKPH
K474 GTKRTLPKPHLEAIV
A479 LPKPHLEAIVENLRT
K563 KQSASGTKRRVFIVE
K614 NVEHMTEKMKTDIQR
H633 RNEKCHEHYTTEFLy
Y634 NEKCHEHYTTEFLyN
Y640-p HYTTEFLyNLYSSEG
Y674 PTPFDRNYGTKLGVK
K689 AMLWVSEKLRDVYRK
K726-ac FSPVTELkKETDFEH
K727 SPVTELkKETDFEHR
T773-p LEHVTRRtLsIDKGF
S775-p HVTRRtLsIDKGF__
  rat

 
K8 MATVDLEKLRMSGAG
K16 LRMSGAGKAIGVLTS
Y43 AVTRMGIYVGAKVFL
T93 SARCKAFTTREGRLA
K144 EELVKEGKISESTAQ
T304 GHVQRGGTPSAFDRV
S377 AIQLRGRSFENNWKI
K469 GGSMLGTKRTLPKPH
K474 GTKRTLPKPHLEAIV
A479 LPKPHLEAIVENLRT
K563 KQSASGTKRRVFIVE
K614 NVEHMTEKMKTDIQR
H633 RNEKCHEHYTTEFLY
Y634 NEKCHEHYTTEFLYN
Y640 HYTTEFLYNLYSSEG
Y674 PTPFDRNYGTKLGVK
K689 AMLWMSEKLRDVYRK
K726 FSSVTELKKETDFEH
K727 SSVTELKKETDFEHR
T773-p LEHVTRRtLsIDKGF
S775-p HVTRRtLsIDKGF__
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