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PFKL (human)

Overview PFKL phosphofructokinase, liver type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Two alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB. Protein type: Kinase, other; Carbohydrate Metabolism - galactose; EC 2.7.1.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - fructose and mannose Cellular Component: 6-phosphofructokinase complex; cytosol Molecular Function: identical protein binding; protein binding; metal ion binding; kinase binding; 6-phosphofructokinase activity; ATP binding Biological Process: fructose 1,6-bisphosphate metabolic process; glycolysis; carbohydrate metabolic process; response to glucose stimulus; glucose metabolic process; fructose 6-phosphate metabolic process; protein oligomerization; negative regulation of insulin secretion; protein homotetramerization Reference #:  P17858 (UniProtKB) Alt. Names/Synonyms: 6-phosphofructokinase, liver type; DKFZp686G1648; DKFZp686L2097; FLJ30173; FLJ40909; K6PL; liver phosphofructokinase; liver-type 1-phosphofructokinase; PFK-B; PFKL; Phosphofructo-1-kinase isozyme B; Phosphofructokinase 1; phosphofructokinase, liver; Phosphohexokinase Gene Symbols: PFKL Molecular weight: 85,018 Da Basal Isoelectric point: 7.26  Predict pI for various phosphorylation states CST Pathways:  Warburg Effect Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below PFKL

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  ENZYME  |  DISEASE 171850 171860  |  Source  |  InnateDB  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human ► Hide Isoforms
0	1		K8	MAAVDLEKLRASGAG
0	2		K16-u	LRASGAGkAIGVLTS
0	1		Y43	AVTRMGIYVGAKVFL
0	1		T93-p	SARCKAFtTREGRRA
0	1		K144-u	EELVAEGkISETTAR
0	2		T304	GHVQRGGTPSAFDRI
0	1		S377	ATQLRGGSFENNWNI
0	1		K469-a	GGSMLGTkRTLPkGQ
0	1		K474-u	GTkRTLPkGQLESIV
0	4		K563-a	KQSASGTkRRVFIVE
0	1		K614-u	NVEHMTEkMKTDIQR
0	21		Y633-p	RNEKCHDyyTTEFLy
0	13		Y634-p	NEKCHDyyTTEFLyN
0	5		Y640-p	yyTTEFLyNLYSSEG
0	1		Y643	TEFLyNLYSSEGKGV
0	3		Y674-p	PTPFDRNyGTKLGVK
0	1		K689-a	AMLWLSEkLREVYRK
0	3		K726-a	FSPVTELkkDTDFEH
0	1		K727-a	SPVTELkkDTDFEHR
1	3		T773	LEHVTRRTLsMDKGF
0	36		S775-p	HVTRRTLsMDKGF__

	PFKL iso2
-	under review
-	gap
Y90-p	AVTRMGIyVGAKVFL
T140	SARCKAFTTREGRRA
K191	EELVAEGKISETTAR
T351	GHVQRGGTPSAFDRI
S424	ATQLRGGSFENNWNI
K516	GGSMLGTKRTLPKGQ
K521	GTKRTLPKGQLESIV
K610	KQSASGTKRRVFIVE
K661	NVEHMTEKMKTDIQR
Y680-p	RNEKCHDyYTTEFLY
Y681	NEKCHDyYTTEFLYN
Y687	yYTTEFLYNLYSSEG
Y690	TEFLYNLYSSEGKGV
Y721	PTPFDRNYGTKLGVK
K736	AMLWLSEKLREVYRK
K773	FSPVTELKKDTDFEH
K774	SPVTELKKDTDFEHR
T820	LEHVTRRTLSMDKGF
S822	HVTRRTLSMDKGF__

	mouse
K8-u	MATVDLEkLRMSGAG
K16	LRMSGAGKAIGVLTS
Y43	AVTRMGIYVGAKVFL
T93	SARCKAFTTREGRLA
K144	EELVKEGKISESTAQ
T304-p	GHVQRGGtPSAFDRI
S377-p	AIQLRGRsFENNWKI
K469	GGSMLGTKRTLPKPH
K474	GTKRTLPKPHLEAIV
K563	KQSASGTKRRVFIVE
K614	NVEHMTEKMKTDIQR
H633	RNEKCHEHYTTEFLy
Y634	NEKCHEHYTTEFLyN
Y640-p	HYTTEFLyNLYSSEG
Y643	TEFLyNLYSSEGRGV
Y674	PTPFDRNYGTKLGVK
K689	AMLWVSEKLRDVYRK
K726	FSPVTELKKETDFEH
K727	SPVTELKKETDFEHR
T773-p	LEHVTRRtLsIDKGF
S775-p	HVTRRtLsIDKGF__

	rat
K8	MATVDLEKLRMSGAG
K16	LRMSGAGKAIGVLTS
Y43	AVTRMGIYVGAKVFL
T93	SARCKAFTTREGRLA
K144	EELVKEGKISESTAQ
T304	GHVQRGGTPSAFDRV
S377	AIQLRGRSFENNWKI
K469	GGSMLGTKRTLPKPH
K474	GTKRTLPKPHLEAIV
K563	KQSASGTKRRVFIVE
K614	NVEHMTEKMKTDIQR
H633	RNEKCHEHyTTEFLY
Y634-p	NEKCHEHyTTEFLYN
Y640	HyTTEFLYNLySSEG
Y643-p	TEFLYNLySSEGRGV
Y674	PTPFDRNYGTKLGVK
K689	AMLWMSEKLRDVYRK
K726	FSSVTELKKETDFEH
K727	SSVTELKKETDFEHR
T773-p	LEHVTRRtLsIDKGF
S775-p	HVTRRtLsIDKGF__

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