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Protein Page:
DDX5 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
DDX5 a putative ATP-dependent RNA helicase. A proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Belongs to the DEAD box helicase family. DDX5/DDX17 subfamily. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; Helicase; EC 3.6.1.-; EC 3.6.4.13; RNA splicing; RNA binding protein
Cellular Component: nucleolus; ribonucleoprotein complex; nucleus
Molecular Function: calmodulin binding; protein binding; enzyme binding; androgen receptor binding; transcription coactivator activity; estrogen receptor binding; calcium-dependent protein binding; RNA helicase activity; ATP-dependent RNA helicase activity; ATP binding
Biological Process: nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; regulation of osteoblast differentiation; in utero embryonic development; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of DNA damage response, signal transduction by p53 class mediator; cell growth; regulation of viral genome replication; regulation of alternative nuclear mRNA splicing, via spliceosome
Reference #:  P17844 (UniProtKB)
Alt. Names/Synonyms: ATP-dependent RNA helicase DDX5; DDX5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD box protein 5; DEAD box-5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); DKFZp434E109; DKFZp686J01190; G17P1; HELR; HLR1; HUMP68; p68; Probable ATP-dependent RNA helicase DDX5; RNA helicase p68
Gene Symbols: DDX5
Molecular weight: 69,148 Da
Basal Isoelectric point: 9.06  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

DDX5
Protein Structure Not Found.


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Sites Implicated In
apoptosis, induced: T446‑p, T564‑p
apoptosis, inhibited: Y593‑p, Y595‑p
cytoskeletal reorganization: Y593‑p
molecular association, regulation: Y593‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

 
0 2 S6-p __MSGYSsDRDRGRD
0 1 S24-p GAPRFGGsRAGPLsG
0 3 S30-p GsRAGPLsGkkFGNP
1 81 K32-a RAGPLsGkkFGNPGE
1 3 K33-a AGPLsGkkFGNPGEk
1 4 K40-a kFGNPGEkLVkkkWN
1 0 K43-a NPGEkLVkkkWNLDE
1 0 K44-a PGEkLVkkkWNLDEL
1 0 K45-a GEkLVkkkWNLDELP
0 1 K45 GEkLVkkKWNLDELP
1 0 K53-a WNLDELPkFEkNFYQ
0 1 K53-u WNLDELPkFEkNFYQ
3 0 K53-s WNLDELPkFEkNFYQ
1 0 K56-a DELPkFEkNFYQEHP
0 3 K56-u DELPkFEkNFYQEHP
1 0 K80-a VETYRRSkEItVRGH
0 3 K80-u VETYRRSkEItVRGH
0 1 T83-p YRRSkEItVRGHNCP
0 1 Y97-p PKPVLNFyEANFPAN
0 1 K144-u VAQTGSGkTLSYLLP
0 1 K197-u YCRACRLksTCIyGG
1 0 S198-p CRACRLksTCIyGGA
0 458 Y202-p RLksTCIyGGAPkGP
0 14 K207-u CIyGGAPkGPQIRDL
0 3 K236-u IDFLECGkTNLRRTT
0 1 Y244-p TNLRRTTyLVLDEAD
0 2 K264-u GFEPQIRkIVDQIRP
0 4 K284-u MWSATWPkEVRQLAE
0 3 Y297-p AEDFLKDyIHINIGA
0 9 K340-u MEEIMSEkENKtIVF
0 1 T344-p MSEkENKtIVFVETk
0 22 K351-a tIVFVETkRRCDELT
0 3 K351-u tIVFVETkRRCDELT
0 1 K375-u AMGIHGDkSQQERDW
0 3 K388-u DWVLNEFkHGkAPIL
0 16 K391-u LNEFkHGkAPILIAT
0 2 K411-u GLDVEDVkFVINyDY
0 4 Y416-p DVkFVINyDYPNSSE
0 14 K437-u GRTARSTkTGTAyTF
0 4 Y442-p STkTGTAyTFFtPNN
1 4 T446-p GTAyTFFtPNNIkQV
0 34 K451-u FFtPNNIkQVSDLIS
0 5 K470-a ANQAINPkLLQLVED
0 20 K470-u ANQAINPkLLQLVED
0 10 S480-p QLVEDRGsGRSRGRG
0 1 Y497 KDDRRDRYsAGkrGG
0 1 S498-p DDRRDRYsAGkrGGF
0 3 K501-a RDRYsAGkrGGFNtF
0 25 R502-m1 DRYsAGkrGGFNtFR
0 3 R502-m2 DRYsAGkrGGFNtFR
0 2 T507-p GkrGGFNtFRDRENy
0 8 Y514-p tFRDRENyDRGySsL
0 19 Y518-p RENyDRGySsLLkRD
0 1 S519 ENyDRGySsLLkRDF
0 10 S520-p NyDRGySsLLkRDFG
0 7 K523-u RGySsLLkRDFGAKT
0 7 Y535-p AKTQNGVySAANYTN
0 1 T541 VySAANYTNGSFGSN
0 1 S544 AANYTNGSFGSNFVS
1 0 T564-p SFRTGNPtGTYQNGY
3 3 Y593-p NGMNQQAyAyPATAA
1 1 Y595-p MNQQAyAyPATAAAP
  mouse

 
S6 __MSSYSSDRDRGRD
S24 GAPRFGGSRTGPLSG
S30 GSRTGPLSGkKFGNP
K32-a RTGPLSGkKFGNPGE
K33 TGPLSGkKFGNPGEK
K40 KFGNPGEKLVKKkWN
K43 NPGEKLVKKkWNLDE
K44 PGEKLVKKkWNLDEL
K45 GEKLVKKKWNLDELP
K45-u GEKLVKKkWNLDELP
K53 WNLDELPKFEkNFYQ
K53 WNLDELPKFEkNFYQ
K53 WNLDELPKFEkNFYQ
K56 DELPKFEKNFYQEHP
K56-u DELPKFEkNFYQEHP
K80 VDTYRRSKEITVRGH
K80 VDTYRRSKEITVRGH
T83 YRRSKEITVRGHNCP
Y97 PKPVLNFYEANFPAN
K144 VAQTGSGKTLSYLLP
K197 YCRACRLKSTCIyGG
- under review  
Y202-p RLKSTCIyGGAPkGP
K207-u CIyGGAPkGPQIRDL
K236 IDFLECGKTNLRRTT
Y244 TNLRRTTYLVLDEAD
K264 GFEPQIRKIVDQIRP
K284-u MWSATWPkEVRQLAE
Y297 AEDFLKDYIHINIGA
K340-u MEEIMSEkENKTIVF
T344 MSEkENKTIVFVETk
K351-a TIVFVETkRRCDELT
K351 TIVFVETKRRCDELT
K375 AMGIHGDKSQQERDW
K388 DWVLNEFKHGkAPIL
K391-u LNEFKHGkAPILIAT
K411 GLDVEDVKFVINYDY
Y416 DVKFVINYDYPNSSE
K437-u GRTARSTkTGTAYTF
Y442 STkTGTAYTFFTPNN
T446 GTAYTFFTPNNIkQV
K451-u FFTPNNIkQVSDLIS
K470 ANQAINPKLLQLVED
K470-u ANQAINPkLLQLVED
S480-p QLVEDRGsGRSRGRG
Y497 KDDRRDRYSAGKrGG
S498 DDRRDRYSAGKrGGF
K501 RDRYSAGKrGGFNTF
R502-m1 DRYSAGKrGGFNTFR
R502 DRYSAGKRGGFNTFR
T507 GKrGGFNTFRDRENY
Y514 TFRDRENYDRGYsNL
Y518 RENYDRGYsNLLkRD
S519-p ENYDRGYsNLLkRDF
N520 NYDRGYsNLLkRDFG
K523-u RGYsNLLkRDFGAKT
Y535 AKTQNGVYSAANYtN
T541-p VYSAANYtNGsFGSN
S544-p AANYtNGsFGSNFVS
T564 SFRTGNPTGTYQNGY
Y593 NGMNQQAYAYPLPQA
Y595 MNQQAYAYPLPQAAP
  rat

 
S6 __MSSYSSDRDRGRD
S24 GAPRFGGSRTGPLSG
S30 GSRTGPLSGKKFGNP
K32 RTGPLSGKKFGNPGE
K33 TGPLSGKKFGNPGEK
K40 KFGNPGEKLVKKKWN
K43 NPGEKLVKKKWNLDE
K44 PGEKLVKKKWNLDEL
K45 GEKLVKKKWNLDELP
K45 GEKLVKKKWNLDELP
K53 WNLDELPKFEKNFYQ
K53 WNLDELPKFEKNFYQ
K53 WNLDELPKFEKNFYQ
K56 DELPKFEKNFYQEHP
K56 DELPKFEKNFYQEHP
K80 VDTYRRSKEITVRGH
K80 VDTYRRSKEITVRGH
T83 YRRSKEITVRGHNCP
Y97 PKPVLNFYEANFPAN
K144 VAQTGSGKTLSYLLP
K197 YCRACRLKSTCIYGG
- under review  
Y202 RLKSTCIYGGAPKGP
K207 CIYGGAPKGPQIRDL
K236 IDFLECGKTNLRRTT
Y244 TNLRRTTYLVLDEAD
K264 GFEPQIRKIVDQIRP
K284 MWSATWPKEVRQLAE
Y297 AEDFLKDYIHINIGA
K340 MEEIMSEKENKTIVF
T344 MSEKENKTIVFVETK
K351 TIVFVETKRRCDELT
K351 TIVFVETKRRCDELT
K375 AMGIHGDKSQQERDW
K388 DWVLNEFKHGKAPIL
K391 LNEFKHGKAPILIAT
K411 GLDVEDVKFVINYDY
Y416 DVKFVINYDYPNSSE
K437 GRTARSTKTGTAYTF
Y442 STKTGTAYTFFTPNN
T446 GTAYTFFTPNNIkQV
K451-u FFTPNNIkQVSDLIS
K470 ANQAINPKLLQLVED
K470 ANQAINPKLLQLVED
S480 QLVEDRGSGRSRGRG
Y497-p KDDRRDRySAGKrGG
S498 DDRRDRySAGKrGGF
K501 RDRySAGKrGGFNTF
R502-m1 DRySAGKrGGFNTFR
R502 DRySAGKRGGFNTFR
T507 GKrGGFNTFRDRENY
Y514 TFRDRENYDRGYSSL
Y518 RENYDRGYSSLLKRD
S519 ENYDRGYSSLLKRDF
S520 NYDRGYSSLLKRDFG
K523 RGYSSLLKRDFGAKT
Y535 AKTQNGVYSAANYTN
T541 VYSAANYTNGSFGSN
S544 AANYTNGSFGSNFVS
T564 SFRTGNPTGTYQNGY
Y593 NGMNQQAYAYPATAA
Y595 MNQQAYAYPATAAAA
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