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Protein Page:
ACTB (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
ACTB Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family. Note: This description may include information from UniProtKB.
Protein type: Cytoskeletal protein; Motility/polarity/chemotaxis
Cellular Component: cortical cytoskeleton; NuA4 histone acetyltransferase complex; cytoskeleton; axon; cytoplasm; cytosol; ribonucleoprotein complex
Molecular Function: protein binding; structural constituent of cytoskeleton; Tat protein binding; kinesin binding; nitric-oxide synthase binding; protein kinase binding; ATP binding
Biological Process: axon guidance; 'de novo' posttranslational protein folding; intercellular junction assembly and maintenance; cellular protein metabolic process; protein folding; response to calcium ion; blood coagulation; cell motility
Reference #:  P60709 (UniProtKB)
Alt. Names/Synonyms: ACTB; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; beta actin; beta cytoskeletal actin; Beta-actin; PS1TP5-binding protein 1; PS1TP5BP1
Gene Symbols: ACTB
Molecular weight: 41,737 Da
Basal Isoelectric point: 5.29  Predict pI for various phosphorylation states
CST Pathways:  Death Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ACTB
Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

 
0 1 D11 DIAALVVDNGsGMCk
0 3 S14-p ALVVDNGsGMCkAGF
0 1 K18-u DNGsGMCkAGFAGDD
1 89 S33-p APRAVFPsIVGRPRH
0 59 K50-a VMVGMGQkDsyVGDE
0 114 K50-u VMVGMGQkDsyVGDE
0 406 S52-p VGMGQkDsyVGDEAQ
0 1059 Y53-p GMGQkDsyVGDEAQs
0 9 S60-p yVGDEAQskRGILtL
0 255 K61-a VGDEAQskRGILtLk
0 119 K61-u VGDEAQskRGILtLk
0 4 T66-p QskRGILtLkyPIEh
0 4 K68-m1 kRGILtLkyPIEhGI
1 0 K68-s kRGILtLkyPIEhGI
0 3 Y69-p RGILtLkyPIEhGIV
1 2 H73-m1 tLkyPIEhGIVTNWD
0 8 T89-p MEKIWHHtFyNELRV
0 852 Y91-p KIWHHtFyNELRVAP
0 85 K113-u TEAPLNPkANREKMT
0 17 Y143-p IQAVLSLyASGRTTG
0 5 S155-p TTGIVMDsGDGVtHT
0 2 T160-p MDsGDGVtHTVPIyE
0 318 Y166-p VtHTVPIyEGyALPH
0 132 Y169-p TVPIyEGyALPHAIL
0 2 T186-p DLAGRDLtDyLMkIL
0 227 Y188-p AGRDLtDyLMkILTE
0 6 K191-a DLtDyLMkILTERGy
0 94 K191-u DLtDyLMkILTERGy
0 851 Y198-p kILTERGysFtttAE
0 23 S199-p ILTERGysFtttAER
0 5 T201-p TERGysFtttAEREI
0 13 T202-p ERGysFtttAEREIV
0 7 T203-p RGysFtttAEREIVR
0 5 K213-a REIVRDIkEkLCyVA
0 4 K213-u REIVRDIkEkLCyVA
0 49 K215-u IVRDIkEkLCyVALD
0 173 Y218-p DIkEkLCyVALDFEQ
0 1 T229 DFEQEMATAASSSSL
0 3 K238-u ASSSSLEksyELPDG
0 11 S239-p SSSSLEksyELPDGQ
0 36 Y240-p SSSLEksyELPDGQV
0 14 T249-p LPDGQVItIGNERFR
0 2 K284-u TTFNSIMkCDVDIRk
2 0 K284-s TTFNSIMkCDVDIRk
0 83 K291-u kCDVDIRkDLyANtV
0 780 Y294-p VDIRkDLyANtVLSG
0 25 T297-p RkDLyANtVLSGGTT
0 41 Y306-p LSGGTTMyPGIADRM
0 9 K315-a GIADRMQkEItALAP
0 103 K315-u GIADRMQkEItALAP
0 5 T318-p DRMQkEItALAPstM
0 6 S323-p EItALAPstMkIkII
0 4 T324-p ItALAPstMkIkIIA
0 10 K326-a ALAPstMkIkIIAPP
0 135 K326-u ALAPstMkIkIIAPP
0 8 K328-a APstMkIkIIAPPER
0 106 K328-u APstMkIkIIAPPER
0 46 Y362-p MWISKQEyDEsGPSI
0 4 S365-p SKQEyDEsGPSIVHR
0 1 K373-a GPSIVHRkCF_____
  mouse

 
D11-ca DIAALVVdNGSGMCK
S14 ALVVdNGSGMCKAGF
K18 dNGSGMCKAGFAGDD
S33-p APRAVFPsIVGRPRH
K50-a VMVGMGQkDsyVGDE
K50-u VMVGMGQkDsyVGDE
S52-p VGMGQkDsyVGDEAQ
Y53-p GMGQkDsyVGDEAQs
S60-p yVGDEAQskRGILtL
K61-a VGDEAQskRGILtLK
K61-u VGDEAQskRGILtLK
T66-p QskRGILtLKyPIEH
K68 kRGILtLKyPIEHGI
K68 kRGILtLKyPIEHGI
Y69-p RGILtLKyPIEHGIV
H73 tLKyPIEHGIVTNWD
T89-p MEKIWHHtFyNELRV
Y91-p KIWHHtFyNELRVAP
K113-u TEAPLNPkANREKMT
Y143-p IQAVLSLyASGRTTG
S155 TTGIVMDSGDGVtHT
T160-p MDSGDGVtHTVPIyE
Y166-p VtHTVPIyEGyALPH
Y169-p TVPIyEGyALPHAIL
T186-p DLAGRDLtDyLMkIL
Y188-p AGRDLtDyLMkILTE
K191-a DLtDyLMkILTERGy
K191-u DLtDyLMkILTERGy
Y198-p kILTERGysFtttAE
S199-p ILTERGysFtttAER
T201-p TERGysFtttAEREI
T202-p ERGysFtttAEREIV
T203-p RGysFtttAEREIVR
K213-a REIVRDIkEkLCyVA
K213 REIVRDIKEkLCyVA
K215-u IVRDIkEkLCyVALD
Y218-p DIkEkLCyVALDFEQ
T229-p DFEQEMAtAASSSSL
K238 ASSSSLEKsyELPDG
S239-p SSSSLEKsyELPDGQ
Y240-p SSSLEKsyELPDGQV
T249-p LPDGQVItIGNERFR
K284 TTFNSIMKCDVDIRk
K284 TTFNSIMKCDVDIRk
K291-u KCDVDIRkDLyANtV
Y294-p VDIRkDLyANtVLSG
T297-p RkDLyANtVLSGGTT
Y306 LSGGTTMYPGIADRM
K315-a GIADRMQkEItALAP
K315-u GIADRMQkEItALAP
T318-p DRMQkEItALAPstM
S323-p EItALAPstMkIkII
T324-p ItALAPstMkIkIIA
K326-a ALAPstMkIkIIAPP
K326-u ALAPstMkIkIIAPP
K328-a APstMkIkIIAPPER
K328-u APstMkIkIIAPPER
Y362 MWISKQEYDEsGPSI
S365-p SKQEYDEsGPSIVHR
K373-a GPSIVHRkCF_____
  rat

 
D11 DIAALVVDNGSGMCK
S14 ALVVDNGSGMCKAGF
K18 DNGSGMCKAGFAGDD
S33-p APRAVFPsIVGRPRH
K50 VMVGMGQKDsyVGDE
K50-u VMVGMGQkDsyVGDE
S52-p VGMGQkDsyVGDEAQ
Y53-p GMGQkDsyVGDEAQS
S60 yVGDEAQSkRGILTL
K61-a VGDEAQSkRGILTLK
K61-u VGDEAQSkRGILTLK
T66 QSkRGILTLKYPIEh
K68 kRGILTLKYPIEhGI
K68 kRGILTLKYPIEhGI
Y69 RGILTLKYPIEhGIV
H73-m1 TLKYPIEhGIVTNWD
T89 MEKIWHHTFyNELRV
Y91-p KIWHHTFyNELRVAP
K113-u TEAPLNPkANREKMT
Y143 IQAVLSLYASGRTTG
S155 TTGIVMDSGDGVTHT
T160 MDSGDGVTHTVPIyE
Y166-p VTHTVPIyEGyALPH
Y169-p TVPIyEGyALPHAIL
T186 DLAGRDLTDyLMkIL
Y188-p AGRDLTDyLMkILTE
K191 DLTDyLMKILTERGy
K191-u DLTDyLMkILTERGy
Y198-p kILTERGysFtTTAE
S199-p ILTERGysFtTTAER
T201-p TERGysFtTTAEREI
T202 ERGysFtTTAEREIV
T203 RGysFtTTAEREIVR
K213 REIVRDIKEkLCyVA
K213 REIVRDIKEkLCyVA
K215-u IVRDIKEkLCyVALD
Y218-p DIKEkLCyVALDFEQ
T229 DFEQEMATAASSSSL
K238 ASSSSLEKsyELPDG
S239-p SSSSLEKsyELPDGQ
Y240-p SSSLEKsyELPDGQV
T249 LPDGQVITIGNERFR
K284 TTFNSIMKCDVDIRK
K284 TTFNSIMKCDVDIRK
K291 KCDVDIRKDLyANTV
Y294-p VDIRKDLyANTVLSG
T297 RKDLyANTVLSGGTT
Y306 LSGGTTMYPGIADRM
K315-a GIADRMQkEITALAP
K315-u GIADRMQkEITALAP
T318 DRMQkEITALAPSTM
S323 EITALAPSTMkIkII
T324 ITALAPSTMkIkIIA
K326-a ALAPSTMkIkIIAPP
K326-u ALAPSTMkIkIIAPP
K328 APSTMkIKIIAPPER
K328-u APSTMkIkIIAPPER
Y362 MWISKQEYDESGPSI
S365 SKQEYDESGPSIVHR
K373 GPSIVHRKCF_____
  cow

 
D11 DIAALVVDNGSGMCK
S14 ALVVDNGSGMCKAGF
K18 DNGSGMCKAGFAGDD
S33 APRAVFPSIVGRPRH
K50 VMVGMGQKDSYVGDE
K50 VMVGMGQKDSYVGDE
S52 VGMGQKDSYVGDEAQ
Y53 GMGQKDSYVGDEAQS
S60 YVGDEAQSKRGILTL
K61 VGDEAQSKRGILTLK
K61 VGDEAQSKRGILTLK
T66 QSKRGILTLKYPIEH
K68 KRGILTLKYPIEHGI
K68 KRGILTLKYPIEHGI
Y69 RGILTLKYPIEHGIV
H73 TLKYPIEHGIVTNWD
T89 MEKIWHHTFYNELRV
Y91 KIWHHTFYNELRVAP
K113 TEAPLNPKANREKMT
Y143 IQAVLSLYASGRTTG
S155 TTGIVMDSGDGVTHT
T160 MDSGDGVTHTVPIYE
Y166 VTHTVPIYEGYALPH
Y169 TVPIYEGYALPHAIL
T186 DLAGRDLTDYLMKIL
Y188 AGRDLTDYLMKILTE
K191 DLTDYLMKILTERGY
K191 DLTDYLMKILTERGY
Y198 KILTERGYSFTTTAE
S199 ILTERGYSFTTTAER
T201 TERGYSFTTTAEREI
T202 ERGYSFTTTAEREIV
T203 RGYSFTTTAEREIVR
K213 REIVRDIKEKLCYVA
K213 REIVRDIKEKLCYVA
K215 IVRDIKEKLCYVALD
Y218 DIKEKLCYVALDFEQ
T229 DFEQEMATAASSSSL
K238 ASSSSLEKSYELPDG
S239 SSSSLEKSYELPDGQ
Y240 SSSLEKSYELPDGQV
T249 LPDGQVITIGNERFR
K284 TTFNSIMKCDVDIRK
K284 TTFNSIMKCDVDIRK
K291 KCDVDIRKDLYANTV
Y294 VDIRKDLYANTVLSG
T297 RKDLYANTVLSGGTT
Y306 LSGGTTMYPGIADRM
K315 GIADRMQKEITALAP
K315 GIADRMQKEITALAP
T318 DRMQKEITALAPSTM
S323 EITALAPSTMKIKII
T324 ITALAPSTMKIKIIA
K326 ALAPSTMKIKIIAPP
K326 ALAPSTMKIKIIAPP
K328 APSTMKIKIIAPPER
K328 APSTMKIKIIAPPER
Y362 MWISKQEYDESGPSI
S365 SKQEYDESGPSIVHR
K373 GPSIVHRKCF_____
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