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Protein Page:
ACTA1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
ACTA1 Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; Cell development/differentiation; Cytoskeletal protein; Contractile protein
Cellular Component: sarcomere; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton
Molecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding
Biological Process: skeletal muscle fiber adaptation; muscle contraction; response to mechanical stimulus; muscle thin filament assembly; response to steroid hormone stimulus; response to lithium ion; response to extracellular stimulus; skeletal muscle fiber development; cell growth; muscle filament sliding
Reference #:  P68133 (UniProtKB)
Alt. Names/Synonyms: ACTA; ACTA1; actin, alpha 1, skeletal muscle; Actin, alpha skeletal muscle; ACTS; alpha skeletal muscle actin; Alpha-actin-1; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3
Gene Symbols: ACTA1
Molecular weight: 42,051 Da
Basal Isoelectric point: 5.23  Predict pI for various phosphorylation states
CST Pathways:  Death Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ACTA1
Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

 
1 89 S35-p APRAVFPsIVGRPRH
0 59 K52-a VMVGMGQkDsyVGDE
0 114 K52-u VMVGMGQkDsyVGDE
0 406 S54-p VGMGQkDsyVGDEAQ
1 0 S54 VGMGQkDSyVGDEAQ
0 1062 Y55-p GMGQkDsyVGDEAQs
0 9 S62-p yVGDEAQskRGILtL
0 255 K63-a VGDEAQskRGILtLk
0 119 K63-u VGDEAQskRGILtLk
0 8 T68-p QskRGILtLkyPIEH
0 7 K70-a kRGILtLkyPIEHGI
0 4 K70-m1 kRGILtLkyPIEHGI
0 45 K70-u kRGILtLkyPIEHGI
0 26 Y71-p RGILtLkyPIEHGII
0 2 T79-p PIEHGIItNWDDMEk
0 14 K86-a tNWDDMEkIWHHtFy
0 9 K86-u tNWDDMEkIWHHtFy
0 10 T91-p MEkIWHHtFyNELRV
0 852 Y93-p kIWHHtFyNELRVAP
0 1 K115-a TEAPLNPkANREKMT
0 15 K115-u TEAPLNPkANREKMT
0 16 Y145-p IQAVLSLyASGRTTG
1 0 S157 TTGIVLDSGDGVTHN
0 91 Y168-p VTHNVPIyEGyALPH
0 74 Y171-p NVPIyEGyALPHAIM
0 2 T188-p DLAGRDLtDyLMkIL
0 226 Y190-p AGRDLtDyLMkILtE
0 6 K193-a DLtDyLMkILtERGy
0 94 K193-u DLtDyLMkILtERGy
0 20 T196-p DyLMkILtERGysFV
0 199 Y200-p kILtERGysFVTTAE
1 6 S201-p ILtERGysFVTTAER
2 0 S201-gl ILtERGysFVTTAER
0 3 T205 RGysFVTTAEREIVR
0 5 K215-a REIVRDIkEkLCyVA
0 1 K217-a IVRDIkEkLCyVALD
0 1 K217-m1 IVRDIkEkLCyVALD
0 1 K217 IVRDIkEKLCyVALD
0 19 Y220-p DIkEkLCyVALDFEN
1 0 S234 NEMATAASsSSLEks
0 3 S235-p EMATAASsSSLEksy
0 3 K240-u ASsSSLEksyELPDG
0 12 S241-p SsSSLEksyELPDGQ
0 35 Y242-p sSSLEksyELPDGQV
0 14 T251-p LPDGQVItIGNERFR
0 2 S267-p PETLFQPsFIGMESA
0 14 Y281-p AGIHETTyNSIMKCD
0 2 K293 KCDIDIRKDLyANNV
1 49 Y296-p IDIRKDLyANNVMSG
0 3 T306-p NVMSGGTtMyPGIAD
0 6 Y308-p MSGGTtMyPGIADRM
0 9 K317-a GIADRMQkEItALAP
0 103 K317-u GIADRMQkEItALAP
0 5 T320-p DRMQkEItALAPstM
0 6 S325-p EItALAPstMkIkII
1 0 S325 EItALAPStMkIkII
0 4 T326-p ItALAPstMkIkIIA
0 10 K328-a ALAPstMkIkIIAPP
0 135 K328-u ALAPstMkIkIIAPP
0 8 K330-a APstMkIkIIAPPER
0 106 K330-u APstMkIkIIAPPER
0 2 T360 TFQQMWITKQEyDEA
0 19 Y364-p MWITKQEyDEAGPSI
1 0 S370 EyDEAGPSIVHRKCF
  mouse

 
S35-p APRAVFPsIVGRPRH
K52-a VMVGMGQkDsyVGDE
K52-u VMVGMGQkDsyVGDE
S54-p VGMGQkDsyVGDEAQ
S54 VGMGQkDSyVGDEAQ
Y55-p GMGQkDsyVGDEAQs
S62-p yVGDEAQskRGILtL
K63-a VGDEAQskRGILtLk
K63-u VGDEAQskRGILtLk
T68-p QskRGILtLkyPIEH
K70 kRGILtLKyPIEHGI
K70 kRGILtLKyPIEHGI
K70-u kRGILtLkyPIEHGI
Y71-p RGILtLkyPIEHGII
T79-p PIEHGIItNWDDMEk
K86 tNWDDMEKIWHHtFy
K86-u tNWDDMEkIWHHtFy
T91-p MEkIWHHtFyNELRV
Y93-p kIWHHtFyNELRVAP
K115 TEAPLNPKANREKMT
K115-u TEAPLNPkANREKMT
Y145-p IQAVLSLyASGRTTG
S157 TTGIVLDSGDGVTHN
Y168-p VTHNVPIyEGyALPH
Y171-p NVPIyEGyALPHAIM
T188-p DLAGRDLtDyLMkIL
Y190-p AGRDLtDyLMkILtE
K193-a DLtDyLMkILtERGy
K193-u DLtDyLMkILtERGy
T196-p DyLMkILtERGysFV
Y200-p kILtERGysFVTtAE
S201-p ILtERGysFVTtAER
S201 ILtERGySFVTtAER
T205-p RGysFVTtAEREIVR
K215-a REIVRDIkEkLCyVA
K217 IVRDIkEKLCyVALD
K217 IVRDIkEKLCyVALD
K217-u IVRDIkEkLCyVALD
Y220-p DIkEkLCyVALDFEN
S234 NEMATAASsSSLEKs
S235-p EMATAASsSSLEKsy
K240 ASsSSLEKsyELPDG
S241-p SsSSLEKsyELPDGQ
Y242-p sSSLEKsyELPDGQV
T251-p LPDGQVItIGNERFR
S267-p PETLFQPsFIGMESA
Y281-p AGIHETTyNSIMKCD
K293-u KCDIDIRkDLyANNV
Y296-p IDIRkDLyANNVMSG
T306-p NVMSGGTtMYPGIAD
Y308 MSGGTtMYPGIADRM
K317-a GIADRMQkEItALAP
K317-u GIADRMQkEItALAP
T320-p DRMQkEItALAPstM
S325-p EItALAPstMkIkII
S325 EItALAPStMkIkII
T326-p ItALAPstMkIkIIA
K328-a ALAPstMkIkIIAPP
K328-u ALAPstMkIkIIAPP
K330-a APstMkIkIIAPPER
K330-u APstMkIkIIAPPER
T360-p TFQQMWItKQEyDEA
Y364-p MWItKQEyDEAGPSI
S370 EyDEAGPSIVHRKCF
  rat

 
S35-p APRAVFPsIVGRPRH
K52 VMVGMGQKDsyVGDE
K52-u VMVGMGQkDsyVGDE
S54-p VGMGQkDsyVGDEAQ
S54-gl VGMGQkDsyVGDEAQ
Y55-p GMGQkDsyVGDEAQS
S62 yVGDEAQSkRGILTL
K63-a VGDEAQSkRGILTLK
K63-u VGDEAQSkRGILTLK
T68 QSkRGILTLKYPIEH
K70 kRGILTLKYPIEHGI
K70 kRGILTLKYPIEHGI
K70 kRGILTLKYPIEHGI
Y71 RGILTLKYPIEHGII
T79 PIEHGIITNWDDMEk
K86 TNWDDMEKIWHHTFy
K86-u TNWDDMEkIWHHTFy
T91 MEkIWHHTFyNELRV
Y93-p kIWHHTFyNELRVAP
K115 TEAPLNPKANREKMT
K115 TEAPLNPKANREKMT
Y145 IQAVLSLYASGRTTG
S157-gl TTGIVLDsGDGVTHN
Y168-p VTHNVPIyEGyALPH
Y171-p NVPIyEGyALPHAIM
T188 DLAGRDLTDyLMkIL
Y190-p AGRDLTDyLMkILTE
K193 DLTDyLMKILTERGy
K193-u DLTDyLMkILTERGy
T196 DyLMkILTERGysFV
Y200-p kILTERGysFVTTAE
S201-p ILTERGysFVTTAER
S201-gl ILTERGysFVTTAER
T205 RGysFVTTAEREIVR
K215 REIVRDIKEKLCyVA
K217 IVRDIKEKLCyVALD
K217 IVRDIKEKLCyVALD
K217 IVRDIKEKLCyVALD
Y220-p DIKEKLCyVALDFEN
S234-gl NEMATAAsSSSLEKs
S235 EMATAAsSSSLEKsy
K240 AsSSSLEKsyELPDG
S241-p sSSSLEKsyELPDGQ
Y242-p SSSLEKsyELPDGQV
T251 LPDGQVITIGNERFR
S267 PETLFQPSFIGMESA
Y281 AGIHETTYNSIMKCD
K293 KCDIDIRKDLyANNV
Y296-p IDIRKDLyANNVMSG
T306 NVMSGGTTMYPGIAD
Y308 MSGGTTMYPGIADRM
K317-a GIADRMQkEITALAP
K317-u GIADRMQkEITALAP
T320 DRMQkEITALAPsTM
S325 EITALAPSTMkIkII
S325-gl EITALAPsTMkIkII
T326 ITALAPsTMkIkIIA
K328-a ALAPsTMkIkIIAPP
K328-u ALAPsTMkIkIIAPP
K330 APsTMkIKIIAPPER
K330-u APsTMkIkIIAPPER
T360 TFQQMWITKQEYDEA
Y364 MWITKQEYDEAGPsI
S370-gl EYDEAGPsIVHRKCF
  rabbit

 
S35 APRAVFPSIVGRPRH
K52 VMVGMGQKDSYVGDE
K52 VMVGMGQKDSYVGDE
S54 VGMGQKDSYVGDEAQ
S54 VGMGQKDSYVGDEAQ
Y55 GMGQKDSYVGDEAQS
S62 YVGDEAQSKRGILTL
K63 VGDEAQSKRGILTLK
K63 VGDEAQSKRGILTLK
T68 QSKRGILTLKYPIEH
K70 KRGILTLKYPIEHGI
K70 KRGILTLKYPIEHGI
K70 KRGILTLKYPIEHGI
Y71 RGILTLKYPIEHGII
T79 PIEHGIITNWDDMEK
K86 TNWDDMEKIWHHTFY
K86 TNWDDMEKIWHHTFY
T91 MEKIWHHTFYNELRV
Y93 KIWHHTFYNELRVAP
K115 TEAPLNPKANREKMT
K115 TEAPLNPKANREKMT
Y145 IQAVLSLYASGRTTG
S157 TTGIVLDSGDGVTHN
Y168 VTHNVPIYEGYALPH
Y171 NVPIYEGYALPHAIM
T188 DLAGRDLTDYLMKIL
Y190 AGRDLTDYLMKILTE
K193 DLTDYLMKILTERGY
K193 DLTDYLMKILTERGY
T196 DYLMKILTERGYSFV
Y200 KILTERGYSFVTTAE
S201 ILTERGYSFVTTAER
S201 ILTERGYSFVTTAER
T205 RGYSFVTTAEREIVR
K215 REIVRDIKEKLCYVA
K217 IVRDIKEKLCYVALD
K217 IVRDIKEKLCYVALD
K217 IVRDIKEKLCYVALD
Y220 DIKEKLCYVALDFEN
S234 NEMATAASSSSLEKS
S235 EMATAASSSSLEKSY
K240 ASSSSLEKSYELPDG
S241 SSSSLEKSYELPDGQ
Y242 SSSLEKSYELPDGQV
T251 LPDGQVITIGNERFR
S267 PETLFQPSFIGMESA
Y281 AGIHETTYNSIMKCD
K293 KCDIDIRKDLYANNV
Y296 IDIRKDLYANNVMSG
T306 NVMSGGTTMYPGIAD
Y308 MSGGTTMYPGIADRM
K317 GIADRMQKEITALAP
K317 GIADRMQKEITALAP
T320 DRMQKEITALAPSTM
S325 EITALAPSTMKIKII
S325 EITALAPSTMKIKII
T326 ITALAPSTMKIKIIA
K328 ALAPSTMKIKIIAPP
K328 ALAPSTMKIKIIAPP
K330 APSTMKIKIIAPPER
K330 APSTMKIKIIAPPER
T360 TFQQMWITKQEYDEA
Y364 MWITKQEYDEAGPSI
S370 EYDEAGPSIVHRKCF
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