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Protein Page:
INCENP (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
INCENP Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1. Homodimer or heterodimer. Interacts with H2AFZ. Interacts with CBX3. Interacts with tubulin beta chain. Interacts with AURKB and AURKC. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC. Interacts with EVI5. Interacts with CDCA8 and BIRC5; interaction is direct. Interacts with CBX5; POGZ and INCENP compete for interaction with CBX5. Interacts with POGZ. Interacts with JTB. Belongs to the INCENP family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Cell cycle regulation
Cellular Component: kinetochore; centric heterochromatin; lateral element; central element; microtubule; protein complex; spindle; midbody; cytosol; chromosome, pericentric region; chromocenter
Molecular Function: protein binding
Biological Process: mitosis; cytokinesis; mitotic cell cycle; chromosome segregation
Reference #:  Q9NQS7 (UniProtKB)
Alt. Names/Synonyms: binds and activates aurora-B and -C in vivo and in vitro; chromosomal passenger protein; FLJ31633; INCE; INCENP; Inner centromere protein; inner centromere protein antigens 135/155kDa; inner centromere protein INCENP; MGC111393
Gene Symbols: INCENP
Molecular weight: 105,429 Da
Basal Isoelectric point: 9.46  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

INCENP

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: T59‑p
activity, induced: T892‑p, S893‑p, S894‑p
intracellular localization: T59‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K29 FLCNMDNKDLVWLEE
0 4 S50 RMFTREFSkEPELMP
0 1 K51-ub MFTREFSkEPELMPK
5 21 T59-p EPELMPKtPSQKNRR
0 29 S72-p RRKKRRIsyVQDENR
0 53 Y73-p RKKRRIsyVQDENRD
0 3 S91-p RRLSRRKsRssQLSS
0 1 S93-p LSRRKsRssQLSSRR
0 5 S94-p SRRKsRssQLSSRRL
0 2 S103-p LSSRRLRsKDsVEKL
0 1 S106-p RRLRsKDsVEKLATV
0 9 S119-p TVVGENGsVLRRVTR
0 1 - gap
0 1 - gap
0 1 P141 ATMALAAPssPtPEs
0 2 S142-p TMALAAPssPtPEsP
0 8 S143-p MALAAPssPtPEsPt
0 1 P144 ALAAPssPtPEsPtM
0 3 T145-p LAAPssPtPEsPtML
0 10 S148-p PssPtPEsPtMLTKK
0 5 T150-p sPtPEsPtMLTKKPE
0 2 T153 PEsPtMLTKKPEDNH
0 1 N178 IGISERQNAEQHVTQ
0 1 Q185 NAEQHVTQLMSTEPL
0 1 T189 HVTQLMSTEPLPRtL
0 2 T195-p STEPLPRtLsPtPAs
0 45 S197-p EPLPRtLsPtPAsAt
0 13 T199-p LPRtLsPtPAsAtAP
0 1 S202-p tLsPtPAsAtAPtsQ
0 2 T204-p sPtPAsAtAPtsQGI
0 2 A205 PtPAsAtAPtsQGIP
0 6 T207-p PAsAtAPtsQGIPts
0 4 S208-p AsAtAPtsQGIPtsD
0 23 T213-p PtsQGIPtsDEEstP
0 30 S214-p tsQGIPtsDEEstPK
0 12 S218-p IPtsDEEstPKKsKA
0 38 T219-p PtsDEEstPKKsKAR
0 4 S223-p EEstPKKsKARILEs
0 2 S230-p sKARILEsItVssLM
0 2 T232-p ARILEsItVssLMAt
0 2 S234-p ILEsItVssLMAtPQ
0 4 S235-p LEsItVssLMAtPQD
0 18 T239-p tVssLMAtPQDPKGQ
0 7 D242 sLMAtPQDPKGQGVG
0 1 T250-p PKGQGVGtGRsAsKL
0 1 S253-p QGVGtGRsAsKLRIA
0 2 S255-p VGtGRsAsKLRIAQV
0 141 S263-p KLRIAQVsPGPRDsP
0 129 S269-p VsPGPRDsPAFPDsP
0 2 F272 GPRDsPAFPDsPWRE
0 1 P273 PRDsPAFPDsPWRER
0 137 S275-p DsPAFPDsPWRERVL
0 8 A283 PWRERVLAPILPDNF
0 1 S291-p PILPDNFstPtGsRt
0 57 T292-p ILPDNFstPtGsRtD
0 1 - gap
0 4 T294-p PDNFstPtGsRtDsQ
0 11 - gap
0 10 S296-p NFstPtGsRtDsQsV
0 7 T298-p stPtGsRtDsQsVRH
0 3 S300-p PtGsRtDsQsVRHsP
0 2 S302-p GsRtDsQsVRHsPIA
0 22 S306-p DsQsVRHsPIAPssP
0 5 S311-p RHsPIAPssPsPQVL
0 11 S312-p HsPIAPssPsPQVLA
0 20 S314-p PIAPssPsPQVLAQk
0 1 K321 sPQVLAQKYSLVAKQ
0 1 K321-ub sPQVLAQkYSLVAKQ
0 1 K327 QkYSLVAKQEsVVRR
0 2 S330-p SLVAKQEsVVRRAsR
0 2 A335 QEsVVRRAsRRLAKK
0 3 S336-p EsVVRRAsRRLAKKT
0 3 S400-p PENNGNNsWPHNDtE
0 1 T406-p NsWPHNDtEIANstP
0 1 S411-p NDtEIANstPNPKPA
1 1 T412-p DtEIANstPNPKPAA
0 1 S420-p PNPKPAAssPEtPSA
0 9 S421-p NPKPAAssPEtPSAG
0 8 T424-p PAAssPEtPSAGQQE
0 15 S446-p GPREPPQsARRKRsY
0 15 S452-p QsARRKRsYKQAVsE
0 3 S458-p RsYKQAVsELDEEQH
0 2 S476-p EELQPPRsKtPssPC
0 6 T478-p LQPPRsKtPssPCPA
0 7 S480-p PPRsKtPssPCPAsk
0 36 S481-p PRsKtPssPCPAskV
0 1 S486-p PssPCPAskVVRPLR
0 1 K487-ac ssPCPAskVVRPLRt
0 1 T494-p kVVRPLRtFLHtVQR
0 1 T498-p PLRtFLHtVQRNQML
0 3 T507-p QRNQMLMtPTsAPRs
0 2 S510-p QMLMtPTsAPRsVMK
0 2 S514-p tPTsAPRsVMKsFIK
0 1 S518-p APRsVMKsFIKRNTP
0 2 N544 KERQRLENLRRKEEA
0 1 K602-ac KKKQIEQkFAQIDEk
0 1 K602-ub KKKQIEQkFAQIDEk
0 1 K609-ac kFAQIDEkTEKAKEE
0 2 K614 DEkTEKAKEERLAEE
0 2 K622 EERLAEEKAKKKAAA
0 1 K630-ac AKKKAAAkkMEEVEA
0 1 K630-m2 AKKKAAAkkMEEVEA
0 1 K631-ac KKKAAAkkMEEVEAR
0 1 K631-m2 KKKAAAkkMEEVEAR
0 1 T793-p ASKALNVtVDVQsPA
0 3 S798-p NVtVDVQsPACtsyQ
0 1 T802-p DVQsPACtsyQMtPQ
0 1 S803-p VQsPACtsyQMtPQG
0 1 Y804-p QsPACtsyQMtPQGH
0 2 T807-p ACtsyQMtPQGHRAP
0 4 Y822-p PKINPDNyGMDLNsD
0 23 S828-p NyGMDLNsDDstDDE
0 24 S831-p MDLNsDDstDDEAHP
0 25 T832-p DLNsDDstDDEAHPR
0 1 A836 DDstDDEAHPRKPIP
0 1 T844-p HPRKPIPtWARGTPL
1 7 T892-p KPRYHKRtssAVWNs
5 26 S893-p PRYHKRtssAVWNsP
6 38 S894-p RYHKRtssAVWNsPP
0 40 S899-p tssAVWNsPPLQGAR
0 9 S909-p LQGARVPssLAysLK
0 3 S910-p QGARVPssLAysLKK
0 6 Y913-p RVPssLAysLKKH__
0 6 S914-p VPssLAysLKKH___
  mouse

 
K29-ub FVCNVDNkDFMWLKE
S50-p RMFIREFsNEPELMP
N51 MFIREFsNEPELMPK
T59-p EPELMPKtPSQKNRR
S72-p RRKKRRVsNIQDENR
N73 RKKRRVsNIQDENRD
S91 KRLSRRKSRSsQVGT
S93 LSRRKSRSsQVGTRH
S94-p SRRKSRSsQVGTRHL
S103 VGTRHLRSKPVTIVE
- gap
F114 TIVEENGFPVLQRIT
S135-p AAAAAAAsVAsAsss
S138-p AAAAsVAsAsssstA
S140-p AAsVAsAsssstAGs
S141-p AsVAsAsssstAGsP
S142-p sVAsAsssstAGsPt
S143-p VAsAsssstAGsPtV
T144-p AsAsssstAGsPtVL
S147-p sssstAGsPtVLtKK
T149-p sstAGsPtVLtKKAV
T152-p AGsPtVLtKKAVVEI
S166-p ISTSERLsAELQLTk
K173-ub sAELQLTkLKGsLPP
S177-p QLTkLKGsLPPsPVS
- gap
- gap
- gap
- gap
P180 kLKGsLPPsPVSQGT
S181-p LKGsLPPsPVSQGTL
V183 GsLPPsPVSQGTLts
S184 sLPPsPVSQGTLtsE
T189-p PVSQGTLtsEEELtP
S190-p VSQGTLtsEEELtPK
L194 TLtsEEELtPKKsEA
T195-p LtsEEELtPKKsEAG
S199-p EELtPKKsEAGKLDS
S206 sEAGKLDSVtVNsLK
T208-p AGKLDSVtVNsLKAt
N210 KLDSVtVNsLKAtPQ
S211-p LDSVtVNsLKAtPQs
T215-p tVNsLKAtPQsPKNR
S218-p sLKAtPQsPKNRGVG
E226 PKNRGVGEGRSVSKL
S229 RGVGEGRSVSKLKIA
S231 VGEGRSVSKLKIARA
S239-p KLKIARAsWGLQDsP
S245-p AsWGLQDsPGstDsP
S248-p GLQDsPGstDsPWQE
T249-p LQDsPGstDsPWQER
S251-p DsPGstDsPWQERVL
S259-p PWQERVLsPILLNNI
L267 PILLNNILPttAksP
P268 ILLNNILPttAksPL
T269-p LLNNILPttAksPLG
T270-p LNNILPttAksPLGN
K272-ub NILPttAksPLGNIR
S273-p ILPttAksPLGNIRS
G276 ttAksPLGNIRSVRR
I278 AksPLGNIRSVRRsL
S280 sPLGNIRSVRRsLIS
S284-p NIRSVRRsLISQDSQ
D289 RRsLISQDSQVPLAS
S290 RsLISQDSQVPLASk
V292 LISQDSQVPLASkYN
K297-ac SQVPLASkYNLVAkQ
K297 SQVPLASKYNLVAkQ
K303-ub SkYNLVAkQENGSRR
N306 NLVAkQENGSRRSSR
S311 QENGSRRSSRRIAKK
S312 ENGSRRSSRRIAKKA
S376 SKNSGCPSKPRSATK
T382 PSKPRSATKIAIStP
S387 SATKIAIStPTSKPA
T388-p ATKIAIStPTSKPAA
A396 PTSKPAAAGQTTTVE
G397 TSKPAAAGQTTTVEE
T399 KPAAAGQTTTVEEQE
S421 GHREPPQSVRRKRSY
S427 QSVRRKRSYKQAISE
S433 RSYKQAISEPDEEQL
N450 EELQPCQNKtPsPPC
T452-p LQPCQNKtPsPPCPA
S454-p PCQNKtPsPPCPANK
P455 CQNKtPsPPCPANKV
N460 PsPPCPANKVVRPLR
K461 sPPCPANKVVRPLRT
T468 KVVRPLRTFLHTVQK
T472 PLRTFLHTVQKNQML
T481-p QKNQMLMtPTLASRS
L484 QMLMtPTLASRSSVM
S489 PTLASRSSVMKSFIK
S493 SRSSVMKSFIKRNTP
S519-p KERQRLEsLRRKEEA
K577 KKKQIEQKFAQIDEK
K577 KKKQIEQKFAQIDEK
K584 KFAQIDEKTEKAkEE
K589-ac DEKTEKAkEERLAEk
K596-ac kEERLAEkAKKKATA
K604 AKKKATAKKMEEVEA
K604 AKKKATAKKMEEVEA
K605 KKKATAKKMEEVEAR
K605 KKKATAKKMEEVEAR
T761 ARKVLNMTVDVQsPV
S766-p NMTVDVQsPVCTSYQ
T770 DVQsPVCTSYQMtPQ
S771 VQsPVCTSYQMtPQG
Y772 QsPVCTSYQMtPQGP
T775-p VCTSYQMtPQGPKSI
Y790 PKISVDDYGMDLNsD
S796-p DYGMDLNsDDstDDE
S799-p MDLNsDDstDDEsHP
T800-p DLNsDDstDDEsHPR
S804-p DDstDDEsHPRKPIP
S812 HPRKPIPSWAKGTQL
T860-p KTRYHKRtssAVWNs
S861-p TRYHKRtssAVWNsP
S862-p RYHKRtssAVWNsPP
S867-p tssAVWNsPPLKATM
S877 LKATMVPSSGD____
S878 KATMVPSSGD_____
- gap
- gap
  rat

 
K29 FVYNVDNKDFMWLKE
N50 RMFVREFNNEPELMP
N51 MFVREFNNEPELMPK
T59 EPELMPKTPSQKNRR
S72 RRKKRRVSNIQDENR
N73 RKKRRVSNIQDENRD
S91 KRLSRRKSRSSQVGP
S93 LSRRKSRSSQVGPRH
S94 SRRKSRSSQVGPRHR
S103 VGPRHRRSKLGAVVE
- gap
S114-p AVVEENGsPVLRRIT
S137 AAASTSASVASASSS
S140 STSASVASASSSPTT
S142 SASVASASSSPTTVS
S143 ASVASASSSPTTVSP
S144 SVASASSSPTTVSPI
P145 VASASSSPTTVSPIV
T146 ASASSSPTTVSPIVL
S149 SSSPTTVSPIVLIKK
I151 SPTTVSPIVLIKKAV
I154 TVSPIVLIKKAVVEV
S168 VSTGEQLSAELQLIK
K175 SAELQLIKLKGGLPQ
G179 QLIKLKGGLPQSSVP
- gap
S183 LKGGLPQSSVPAPAT
V185 GGLPQSSVPAPATSS
P188 PQSSVPAPATSSVSQ
T190 SSVPAPATSSVSQGT
S191 SVPAPATSSVSQGTL
V193 PAPATSSVSQGTLTS
S194 APATSSVSQGTLTSE
T199 SVSQGTLTSEEEAtP
S200 VSQGTLTSEEEAtPK
A204 TLTSEEEAtPKKSEA
T205-p LTSEEEAtPKKSEAG
S209 EEAtPKKSEAGRLDS
S220 RLDSATDSVAVNSLK
A222 DSATDSVAVNSLKAT
N224 ATDSVAVNSLKATPQ
S225 TDSVAVNSLKATPQS
T229 AVNSLKATPQSPKNR
S232 SLKATPQSPKNRGVG
E240 PKNRGVGEARSVSKL
S243 RGVGEARSVSKLKIA
S245 VGEARSVSKLKIARA
S253 KLKIARASRGLQDsP
S259-p ASRGLQDsPGSTDsP
S262 GLQDsPGSTDsPWQE
T263 LQDsPGSTDsPWQER
S265-p DsPGSTDsPWQERVL
S273-p PWQERVLsPILPNNI
L281 PILPNNILSTTAKNP
S282 ILPNNILSTTAKNPL
T283 LPNNILSTTAKNPLG
T284 PNNILSTTAKNPLGS
K286 NILSTTAKNPLGSIQ
N287 ILSTTAKNPLGSIQS
G290 TTAKNPLGSIQSVQQ
I292 AKNPLGSIQSVQQSL
S294 NPLGSIQSVQQSLIS
S298 SIQSVQQSLISQDSQ
D303 QQSLISQDSQVPLAK
S304 QSLISQDSQVPLAKK
V306 LISQDSQVPLAKKYC
K311 SQVPLAKKYCLVTQQ
K311 SQVPLAKKYCLVTQQ
Q317 KKYCLVTQQENASRR
N320 CLVTQQENASRRssR
S325-p QENASRRssRIAKKA
S326-p ENASRRssRIAKKAG
S389 SKNSGCSSKPHSATK
T395 SSKPHSATKMAISTP
S400 SATKMAISTPTSKPV
T401 ATKMAISTPTSKPVA
T409 PTSKPVATGQATAVE
G410 TSKPVATGQATAVEE
A412 KPVATGQATAVEEQD
S434 GHREPPQSVRRKRSY
S440 QSVRRKRSYKQAMSE
S446 RSYKQAMSELDEEHL
N463 EELQPPRNKtPSPPC
T465-p LQPPRNKtPSPPCPA
S467 PPRNKtPSPPCPASK
P468 PRNKtPSPPCPASKV
S473 PSPPCPASKVVRPLR
K474 SPPCPASKVVRPLRT
T481 KVVRPLRTFLHTVQK
T485 PLRTFLHTVQKNQML
T494 QKNQMLMTPTLASRS
L497 QMLMTPTLASRSSVM
S502 PTLASRSSVMKSFIK
S506 SRSSVMKSFIKRNTP
S528 KERQRLESLRRKEEA
K586 KKKQIEQKFAQIDEK
K586 KKKQIEQKFAQIDEK
K593 KFAQIDEKTEKAKEE
K598 DEKTEKAKEERLAEK
K605 KEERLAEKAKKKAAA
K613 AKKKAAAKKMEEMEA
K613 AKKKAAAKKMEEMEA
K614 KKKAAAKKMEEMEAR
K614 KKKAAAKKMEEMEAR
T777 AGKALNMTVDVQSPV
S782 NMTVDVQSPVCTSYQ
T786 DVQSPVCTSYQMTPQ
S787 VQSPVCTSYQMTPQG
Y788 QSPVCTSYQMTPQGP
T791 VCTSYQMTPQGPKSF
Y806 PKISMDDYGMDLNSD
S812 DYGMDLNSDDSTDDE
S815 MDLNSDDSTDDEAHP
T816 DLNSDDSTDDEAHPR
A820 DDSTDDEAHPRKPIP
S828 HPRKPIPSWAKGTQL
T876 KTRYHKRTSSAVWNS
S877 TRYHKRTSSAVWNSP
S878 RYHKRTSSAVWNSPP
S883 TSSAVWNSPPLKATI
S892 PLKATIPSSGD____
S893 LKATIPSSGD_____
- gap
- gap
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