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Protein Page:
NHERF (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NHERF a scaffold protein that connects plasma membrane proteins with members of the Ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized beta-2-adrenergic receptor Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold
Cellular Component: centrosome; microvillus; intracellular membrane-bound organelle; brush border membrane; endomembrane system; lipid raft; actin cytoskeleton; ruffle; membrane; microvillus membrane; apical plasma membrane; cytoplasm; plasma membrane; nucleus; filopodium
Molecular Function: protein binding; chloride channel regulator activity; protein self-association; beta-2 adrenergic receptor binding; protein complex binding; beta-catenin binding; dopamine receptor binding; protein complex scaffold; PDZ domain binding; phosphatase binding; receptor binding
Biological Process: bile acid secretion; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; renal sodium ion transport; microvillus biogenesis; negative regulation of cell proliferation; cellular phosphate ion homeostasis; regulation of sodium:hydrogen antiporter activity; protein complex assembly; dopamine receptor, phospholipase C activating pathway; actin cytoskeleton organization and biogenesis; regulation of protein kinase activity; negative regulation of sodium:hydrogen antiporter activity; negative regulation of cell migration; negative regulation of phosphoinositide 3-kinase cascade; dopamine receptor, adenylate cyclase activating pathway
Reference #:  O14745 (UniProtKB)
Alt. Names/Synonyms: EBP50; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF; NHERF-1; NHERF1; NHRF1; NPHLOP2; Regulatory cofactor of Na(+)/H(+) exchanger; SLC9A3R1; Sodium-hydrogen exchanger regulatory factor 1; sodium/hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1; Solute carrier family 9 isoform A3 regulatory factor 1
Gene Symbols: SLC9A3R1
Molecular weight: 38,868 Da
Basal Isoelectric point: 5.55  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NHERF

Protein Structure Not Found.


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Sites Implicated In
intracellular localization: S77‑p
molecular association, regulation: S77‑p, S162‑p, S339‑p, S340‑p
phosphorylation: T95‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 6 S2-p ______MsADAAAGA
0 1 K34-ac HLHGEKGkLGQyIRL
0 1 K34 HLHGEKGKLGQyIRL
0 9 Y38-p EKGkLGQyIRLVEPG
0 5 S46-p IRLVEPGsPAEkAGL
0 5 K50-ub EPGsPAEkAGLLAGD
0 1 K69 VNGENVEKETHQQVV
4 0 S77-p ETHQQVVsRIRAALN
2 0 T95-p LLVVDPEtDEQLQkL
0 9 K101-ub EtDEQLQkLGVQVRE
0 2 A116 ELLRAQEAPGQAEPP
0 2 P117 LLRAQEAPGQAEPPA
0 1 K142 NEPREADKSHPEQRE
0 2 T156-p ELRPRLCtMKKGPsG
2 3 S162-p CtMKKGPsGyGFNLH
0 2 Y164-p MKKGPsGyGFNLHsD
0 2 S170-p GyGFNLHsDKsKPGQ
0 2 S173-p FNLHsDKsKPGQFIR
0 1 K174 NLHsDKsKPGQFIRs
0 1 S181-p KPGQFIRsVDPDSPA
0 1 S186 IRsVDPDSPAEAsGL
0 1 S191-p PDSPAEAsGLRAQDR
0 1 S217-p KQHGDVVsAIRAGGD
0 1 R220 GDVVsAIRAGGDETK
0 1 R233 TKLLVVDRETDEFFK
0 1 R233 TKLLVVDRETDEFFK
0 1 K240 RETDEFFKKCRVIPS
0 1 K266 FTNGEIQKENsREAL
0 1 N268 NGEIQKENsREALAE
0 10 S269-p GEIQKENsREALAEA
0 10 L278 EALAEAALEsPRPAL
2 161 S280-p LAEAALEsPRPALVR
1 75 S288-p PRPALVRsAssDtsE
4 124 S290-p PALVRsAssDtsEEL
1 51 S291-p ALVRsAssDtsEELN
0 41 T293-p VRsAssDtsEELNsQ
0 24 S294-p RsAssDtsEELNsQD
0 12 S299-p DtsEELNsQDsPPKQ
2 66 S302-p EELNsQDsPPKQDST
0 3 S308 DsPPKQDSTAPSsTS
0 3 T309 sPPKQDSTAPSsTSS
0 1 S313-p QDSTAPSsTSSSDPI
3 19 S339-p ERAHQKRssKRAPQM
3 13 S340-p RAHQKRssKRAPQMD
  mouse

 
S2-p ______MsADAAAGE
K34 HLHGEKGKVGQFIRL
K34-ub HLHGEKGkVGQFIRL
F38 EKGkVGQFIRLVEPG
S46 IRLVEPGSPAEkSGL
K50-ub EPGSPAEkSGLLAGD
K69-ub VNGENVEkETHQQVV
S77-p ETHQQVVsRIRAALN
T95 LLVVDPETDERLKkL
K101-ub ETDERLKkLGVSIRE
K116-ub ELLRPQEksEQAEPP
S117-p LLRPQEksEQAEPPA
K139-ub GDQNEAEkSHLRELR
T151 ELRPRLCTMKKGPNG
N157 CTMKKGPNGYGFNLH
Y159 MKKGPNGYGFNLHSD
S165 GYGFNLHSDKSkPGQ
S168 FNLHSDKSkPGQFIR
K169-ub NLHSDKSkPGQFIRA
A176 kPGQFIRAVDPDsPA
S181-p IRAVDPDsPAEASGL
S186 PDsPAEASGLRAQDR
S212 KQHGDVVSAIkGGGD
K215-ub GDVVSAIkGGGDEAK
K228-ub AKLLVVDkETDEFFk
K228-ac AKLLVVDkETDEFFk
K235-ac kETDEFFkKCKVIPS
K261-ub FSNGEIQkEssREAL
S263-p NGEIQkEssREALVE
S264-p GEIQkEssREALVEP
S273-p EALVEPAsEsPRPAL
S275-p LVEPAsEsPRPALAR
S283-p PRPALARsAssDtsE
S285-p PALARsAssDtsEEL
S286-p ALARsAssDtsEELN
T288-p ARsAssDtsEELNsQ
S289-p RsAssDtsEELNsQD
S294-p DtsEELNsQDsPKRQ
S297-p EELNsQDsPKRQVst
S303-p DsPKRQVstEPSSTS
T304-p sPKRQVstEPSSTSS
S308 QVstEPSSTSSSSSD
S336 ERAHQKRSSKRAPQM
S337 RAHQKRSSKRAPQMD
  rat

 
S2 ______MSADAAAGE
K34 HLHGEKGKVGQFIRL
K34 HLHGEKGKVGQFIRL
F38 EKGKVGQFIRLVEPG
S46 IRLVEPGSPAEKSGL
K50 EPGSPAEKSGLLAGD
K69 VNGENVEKETHQQVV
S77 ETHQQVVSRIRAALN
T95 LLVVDPETDEQLKKL
K101 ETDEQLKKLGVPIRE
K116-ub ELLRAQEkSEHTEPP
S117 LLRAQEkSEHTEPPA
K139 GDQNEAEKSHLERCE
T153 ELRPRLCTMKKGPNG
N159 CTMKKGPNGYGFNLH
Y161 MKKGPNGYGFNLHsD
S167-p GYGFNLHsDKSKPGQ
S170 FNLHsDKSKPGQFIR
K171 NLHsDKSKPGQFIRA
A178 KPGQFIRAVDPDSPA
S183 IRAVDPDSPAEASGL
S188 PDSPAEASGLRAQDR
S214 KQHGDVVSAIKAGGD
K217 GDVVSAIKAGGDEAK
K230 AKLLVVDKETDEFFK
K230 AKLLVVDKETDEFFK
K237 KETDEFFKKCRVTPS
K263 FSNGEIQKENSREAL
N265 NGEIQKENSREALVE
S266 GEIQKENSREALVEP
S275-p EALVEPAsEsPRPAL
S277-p LVEPAsEsPRPALAR
S285-p PRPALARsAssDtsE
S287-p PALARsAssDtsEEL
S288-p ALARsAssDtsEELN
T290-p ARsAssDtsEELNAQ
S291-p RsAssDtsEELNAQD
A296 DtsEELNAQDsPKRH
S299-p EELNAQDsPKRHDST
S305 DsPKRHDSTEPSSTS
T306 sPKRHDSTEPSSTSS
S310 HDSTEPSSTSSSSDP
S337-p ERAHQKRssKRAPQM
S338-p RAHQKRssKRAPQMD
  rabbit

 
S2 ______MSADAAAGA
K34 HLHGEKGKVGQYIRL
K34 HLHGEKGKVGQYIRL
Y38 EKGKVGQYIRLVEPG
S46 IRLVEPGSPAEKAGL
K50 EPGSPAEKAGLLAGD
K69 VNGENVEKETHQQVV
S77 ETHQQVVSRIRAALN
T95 LLVVDPDTDEQFRKL
K101 DTDEQFRKLGVQIRG
G116 ELLRAQAGPEQAGPP
P117 LLRAQAGPEQAGPPA
K142 NEPREVEKSHPERRE
A156 ELRPRLCAMKKGPNG
N162 CAMKKGPNGYGFNLH
Y164 MKKGPNGYGFNLHSD
S170 GYGFNLHSDKSRPGQ
S173 FNLHSDKSRPGQFIR
R174 NLHSDKSRPGQFIRA
A181 RPGQFIRAVDPDSPA
S186 IRAVDPDSPAEASGL
S191 PDSPAEASGLREQDR
T217 KQHGDVVTAIKAGGD
K220 GDVVTAIKAGGDEAK
K233 AKLLVVDKETDEFFK
K233 AKLLVVDKETDEFFK
K240 KETDEFFKKCKVVPS
K266 FTNGEIQKNNPETLA
N267 TNGEIQKNNPETLAP
N268 NGEIQKNNPETLAPA
S277 ETLAPAASESPRPAL
S279 LAPAASESPRPALAR
S287-p PRPALARsAssDTSE
S289-p PALARsAssDTSEEL
S290-p ALARsAssDTSEELA
T292 ARsAssDTSEELASQ
S293 RsAssDTSEELASQD
S298 DTSEELASQDSPKKE
S301 EELASQDSPKKEDST
S307 DSPKKEDSTAPSSTS
T308 SPKKEDSTAPSSTSS
S312 EDSTAPSSTSSSSDP
S339 ERAHQKRSSRRAPQM
S340 RAHQKRSSRRAPQMD
  dog

 
S2 ______MSADAAAGA
K34 HLHGEKGKVGQFIRL
K34 HLHGEKGKVGQFIRL
F38 EKGKVGQFIRLVEPG
S46 IRLVEPGSPAEKAGL
K50 EPGSPAEKAGLLAGD
K69 VNGENVEKETHQQVV
S77 ETHQQVVSRIRAALN
A95 LLVVDPDADERLQKL
K101 DADERLQKLGVPVRE
G116 ELLRAQDGPGQAEPP
P117 LLRAQDGPGQAEPPA
R149 PEPREAERSGPERRE
A163 ELRPRLCAMKKGANG
N169 CAMKKGANGYGFNLH
Y171 MKKGANGYGFNLHSD
S177 GYGFNLHSDKSKPGQ
S180 FNLHSDKSKPGQFIR
K181 NLHSDKSKPGQFIRA
A188 KPGQFIRAVDPDSPA
S193 IRAVDPDSPAEASGL
S198 PDSPAEASGLRAQDR
S225 KQHGDVVSAIKAGGD
K228 GDVVSAIKAGGDETK
K241 TKLLVVDKETDEFFK
K241 TKLLVVDKETDEFFK
K248 KETDEFFKKCKVIPS
K274 FTNGEIRKENSREPL
N276 NGEIRKENSREPLAE
S277 GEIRKENSREPLAEV
S286 EPLAEVASESPRSAL
S288 LAEVASESPRSALAR
S296 PRSALARSTSSDTSE
S298 SALARSTSSDTSEEL
S299 ALARSTSSDTSEELN
T301 ARSTSSDTSEELNSQ
S302 RSTSSDTSEELNSQD
S307 DTSEELNSQDSPRKQ
S310 EELNSQDSPRKQDST
S316 DSPRKQDSTAPSSTS
T317 SPRKQDSTAPSSTSS
S321 QDSTAPSSTSSSSDP
S348-p ERAHQKRssKRAPQM
S349-p RAHQKRssKRAPQMD
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