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Protein Page:
5-LO (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
5-LO arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer. Note: This description may include information from UniProtKB.
Protein type: Oxidoreductase; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; Nuclear envelope; EC 1.13.11.34
Cellular Component: extracellular space; nuclear membrane; nuclear matrix; nuclear envelope lumen; nuclear envelope; cytosol
Molecular Function: protein binding; iron ion binding; arachidonate 5-lipoxygenase activity
Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process
Reference #:  P09917 (UniProtKB)
Alt. Names/Synonyms: 5-lipoxygenase; 5-LO; 5-LOX; 5LPG; ALOX5; Arachidonate 5-lipoxygenase; arachidonic acid 5-lipoxygenase; leukotriene A4 synthase; LOG5; LOX5; MGC163204
Gene Symbols: ALOX5
Molecular weight: 77,983 Da
Basal Isoelectric point: 5.51  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

5-LO

Protein Structure Not Found.


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Sites Implicated In
enzymatic activity, induced: S272‑p, S664‑p
enzymatic activity, inhibited: S524‑p
intracellular localization: S272‑p, S524‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 0 Y21-p WFAGTDDyIyLSLVG
1 0 Y23-p AGTDDyIyLSLVGSA
1 0 Y43-p HLLDKPFyNDFERGA
1 0 Y54-p ERGAVDSyDVTVDEE
0 1 Y75-p VRIEKRKyWLNDDWY
0 1 T87-p DWYLKYItLKTPHGD
1 0 Y95-p LKTPHGDyIEFPCyR
1 0 Y101-p DyIEFPCyRWItGDV
0 1 T105-p FPCyRWItGDVEVVL
0 1 Y182-p GVDFVLNysKAMENL
0 1 S183-p VDFVLNysKAMENLF
1 0 T250-p PVLIRRCtELPEKLP
1 0 T259-p LPEKLPVttEMVECs
1 0 T260-p PEKLPVttEMVECsL
1 0 S266-p ttEMVECsLERQLsL
7 2 S272-p CsLERQLsLEQEVQQ
0 1 T298 DGIDANKTDPCTLQF
0 1 T302 ANKTDPCTLQFLAAP
1 0 T445-p QRAMKDLtyAsLCFP
1 0 Y446-p RAMKDLtyAsLCFPE
1 0 S448-p MKDLtyAsLCFPEAI
0 1 K456 LCFPEAIKARGMESK
1 0 T486-p WEAIRTFtAEVVDIY
4 0 S524-p GMRGRKSsGFPkSVK
0 1 K528-ac RKSsGFPkSVKsREQ
0 1 S532-p GFPkSVKsREQLSEY
3 0 S664-p QLPYYYLsPDRIPNs
1 0 S671-p sPDRIPNsVAI____
3748 : Phospho-5-Lipoxygenase (Ser271) Antibody
3749 : Phospho-5-Lipoxygenase (Ser663) Antibody
  mouse

 
Y21 WFAGTDDYIYLSLIG
Y23 AGTDDYIYLSLIGSA
Y43 HLLDKAFYNDFERGA
Y54 ERGAVDSYDVTVDEE
Y75 VKIEKRKYWLHDDWY
T87 DWYLKYITLKTPHGD
Y95 LKTPHGDYIEFPCYR
Y101 DYIEFPCYRWITGEG
T105 FPCYRWITGEGEIVL
Y182 GVDFVLNYSKAMENL
S183 VDFVLNYSKAMENLF
T250 PVLIKRCTALPPKLP
T259 LPPKLPVTTEMVECS
T260 PPKLPVTTEMVECSL
S266 TTEMVECSLERQLsL
S272-p CSLERQLsLEQEVQE
T298-p DGIDANKtDPCtHQF
T302-p ANKtDPCtHQFLAAP
T445 QRAVQDLTYSSLCFP
Y446 RAVQDLTYSSLCFPE
S448 VQDLTYSSLCFPEAI
K456-ub LCFPEAIkARGMDST
T486 WEAIQSFTMEVVSIY
S524 GMRGKKASGFPKSIK
K528 KKASGFPKSIKSREK
S532 GFPKSIKSREKLSEY
S664 KLPYYYLSPDRIPNS
S671 SPDRIPNSVAI____
3748 : Phospho-5-Lipoxygenase (Ser271) Antibody
3749 : Phospho-5-Lipoxygenase (Ser663) Antibody
  rat

 
Y21 WFAGTDDYIYLSLIG
Y23 AGTDDYIYLSLIGSA
Y43 HLLDKAFYNDFERGG
Y54 ERGGRDSYDVTVDEE
Y75 VKIEKRKYRLHDDWY
T87 DWYLKYITLKTPHDY
Y94 TLKTPHDYIEFPCYR
Y100 DYIEFPCYRWITGEG
T104 FPCYRWITGEGEIVL
Y181 GVDFVLNYSKAMENL
S182 VDFVLNYSKAMENLF
T249 PVLIKRCTELPKKLP
T258 LPKKLPVTTEMVECS
T259 PKKLPVTTEMVECSL
S265 TTEMVECSLERQLSL
S271 CSLERQLSLEQEVQE
T297 DGIDANKTDPCTHQF
T301 ANKTDPCTHQFLAAP
T444 QRAVQDLTYSSLCFP
Y445 RAVQDLTYSSLCFPE
S447 VQDLTYSSLCFPEAI
K455 LCFPEAIKARGMDNT
T485 WEAIQSFTTEVVSIY
S523-p GMRGRKAsGFPKSIK
K527 RKAsGFPKSIKSREK
S531 GFPKSIKSREKLSEY
S663 KLPYYYLSPDRIPNS
S670 SPDRIPNSVAI____
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