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Protein Page:
NFkB-p100 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NFkB-p100 a transcription factor of the nuclear factor-kappaB ( NFkB) group. Precursor of the p52 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. The precursor protein itself does not bind to DNA. LT-beta receptor agonists and LPS induce NF-kappaB/p100 processing to p52 at the level of the ribosome. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two spliced isoforms have been identified. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor
Cellular Component: Bcl3/NF-kappaB2 complex; nucleoplasm; cytoplasm; nucleolus; cytosol; nucleus
Molecular Function: protein binding; DNA binding; transcription coactivator activity; transcription factor activity
Biological Process: spleen development; extracellular matrix organization and biogenesis; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; follicular dendritic cell differentiation; toll-like receptor 3 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of transcription, DNA-dependent; positive regulation of interferon type I production; toll-like receptor signaling pathway; germinal center formation; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway
Reference #:  Q00653 (UniProtKB)
Alt. Names/Synonyms: DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; LYT-10; LYT10; NFKB2; Nuclear factor NF-kappa-B p100 subunit; Nuclear factor NF-kappa-B p52 subunit; nuclear factor of kappa light chain gene enhancer in B-cells 2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100); Oncogene Lyt-10; p52
Gene Symbols: NFKB2
Molecular weight: 96,749 Da
Basal Isoelectric point: 5.84  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  B Cell Receptor Signaling  |  Death Receptor Signaling  |  Inhibition of Apoptosis  |  NF-kB Signaling  |  T Cell Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NFkB-p100

Protein Structure Not Found.


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Sites Implicated In
transcription, altered: S222‑p
molecular association, regulation: S222‑p, S866‑p, S870‑p
protein degradation: S707‑p, S711‑p
protein processing: S866‑p, S870‑p
ubiquitination: S707‑p, S711‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 D10-ca SCYNPGLdGIIEYDD
0 2 S23-p DDFKLNSsIVEPKEP
0 2 Y55-p QRGFRFRyGCEGPSH
0 1 K72 LPGASSEKGRKTYPT
1 0 K90-sm CNYEGPAkIEVDLVt
0 1 T97-p kIEVDLVtHsDPPRA
1 1 S99-p EVDLVtHsDPPRAHA
1 0 S108-p PPRAHAHsLVGKQCs
1 0 S115-p sLVGKQCsELGICAV
1 0 S123-p ELGICAVsVGPKDMT
0 7 S161-p LQRQRLRsRPQGLTE
0 1 S195 SIVRLRFSAFLRASD
1 2 S222-p QPIHDSKsPGASNLK
0 1 S277-p WQAFGDFsPTDVHKQ
0 9 Y285-p PTDVHKQyAIVFRTP
1 0 K298-sm TPPYHKMkIERPVTV
0 1 K321-ub GGDVSDSkQFTyyPL
0 1 Y325-p SDSkQFTyyPLVEDK
0 1 Y326-p DSkQFTyyPLVEDKE
0 1 S413 QMAATVPSRDSGEEA
0 13 A425 EEAAEPSAPSRtPQC
0 2 T429-p EPSAPSRtPQCEPQA
0 1 M439 CEPQAPEMLQRAREY
0 1 T651-p MEELGLVtHLVtKLR
0 1 T655-p GLVtHLVtKLRANVN
0 2 T682-p AAGLGYPtLTRLLLk
1 0 K689-sm tLTRLLLkAGADIHA
2 1 S707-p EPLCPLPsPPTsDsD
2 0 S711-p PLPsPPTsDsDsDsE
1 0 S713-p PsPPTsDsDsDsEGP
1 1 S715-p PPTsDsDsDsEGPEK
1 0 S717-p TsDsDsDsEGPEKDT
0 1 S727-p PEKDTRSsFRGHTPL
0 9 K741-ub LDLTCSTkVkTLLLN
0 1 K743-ub LTCSTkVkTLLLNAA
0 2 S812-p DTYRQTTsPSGSLLR
1 0 K855-ub RGPETRDkLPstAEV
0 4 S858-p ETRDkLPstAEVkED
0 2 T859-p TRDkLPstAEVkEDs
1 0 K863-sm LPstAEVkEDsAyGs
5 1 S866-p tAEVkEDsAyGsQsV
0 4 Y868-p EVkEDsAyGsQsVEQ
5 2 S870-p kEDsAyGsQsVEQEA
2 1 S872-p DsAyGsQsVEQEAEK
4810 : Phospho-NF-KappaB2 p100 (Ser866/870) Antibody
4810 : Phospho-NF-KappaB2 p100 (Ser866/870) Antibody
  NFkB-p100 iso4  
D10 SCYNPGLDGIIEYDD
S23 DDFKLNSSIVEPKEP
Y55 QRGFRFRYGCEGPSH
K72 LPGASSEKGRKTYPT
K90 CNYEGPAKIEVDLVT
T97 KIEVDLVTHSDPPRA
S99 EVDLVTHSDPPRAHA
S108 PPRAHAHSLVGKQCS
S115 SLVGKQCSELGICAV
S123 ELGICAVSVGPKDMT
S161 LQRQRLRSRPQGLTE
S195 SIVRLRFSAFLRASD
S222 QPIHDSKSPGASNLK
S277 WQAFGDFSPTDVHKQ
Y285 PTDVHKQYAIVFRTP
K298 TPPYHKMKIERPVTV
K321 GGDVSDSKQFTYYPL
Y325 SDSKQFTYYPLVEDK
Y326 DSKQFTYYPLVEDKE
S413 QMAATVPSRDSGEEA
A425 EEAAEPSAPSRTPQC
T429 EPSAPSRTPQCEPQA
M439 CEPQAPEMLQRAREY
T651 MEELGLVTHLVTKLR
T655 GLVTHLVTKLRANVN
T682 AAGLGYPTLTRLLLK
K689 TLTRLLLKAGADIHA
S707 EPLCPLPSPPTSDSD
S711 PLPSPPTSDSDSDSE
S713 PSPPTSDSDSDSEGP
S715 PPTSDSDSDSEGPEK
S717 TSDSDSDSEGPEKDT
S727 PEKDTRSSFRGHTPL
K741 LDLTCSTKVKTLLLN
K743 LTCSTKVKTLLLNAA
S812 DTYRQTTSPSGSLLR
K855 RGPETRDKLPSTEVK
S858 ETRDKLPSTEVKEDS
T859 TRDKLPSTEVKEDSA
K862 KLPSTEVKEDSAyGS
S865 STEVKEDSAyGSQSV
Y867-p EVKEDSAyGSQSVEQ
S869 KEDSAyGSQSVEQEA
S871 DSAyGSQSVEQEAEK
  mouse

 
D10 NCYDPGLDGIPEYDD
S23 DDFEFSPSIVEPKDP
Y55 QRGFRFRYGCEGPSH
K72-ac LPGASSEkGRKTYPT
K90 CNYEGPAKIEVDLVT
T97 KIEVDLVTHSDPPRA
S99 EVDLVTHSDPPRAHA
S108 PPRAHAHSLVGKQCS
S115 SLVGKQCSELGVCAV
S123 ELGVCAVSVGPKDMT
S161 LQRQRLRSKPQGLTE
S195-p SIVRLRFsAFLRASD
S222 QPIHDSKSPGASNLK
S277 WQAFGDFSPTDVHKQ
Y285 PTDVHKQYAIVFRTP
K298 TPPYHKMKIERPVTV
K321 GGDVSDSKQFTYYPL
Y325 SDSKQFTYYPLVEDK
Y326 DSKQFTYYPLVEDKE
T414-p MAGSRRDtDAGEGAE
T425-p EGAEEPRtPPEAPQG
A429 EPRtPPEAPQGEPQA
T439-p GEPQALDtLQRAREY
T651 MEELGLVTHLVTKLH
T655 GLVTHLVTKLHANVN
T682 AAGLGSPTLTRLLLK
K689 TLTRLLLKAGADIHA
S707-p EPLCPLPsPSTSGSD
S711 PLPsPSTSGSDsDSE
S713 PsPSTSGSDsDSEGP
S715-p PSTSGSDsDSEGPER
S717 TSGSDsDSEGPERDT
N727 PERDTQRNFRGHTPL
K741 LDLTCSTKVKTLLLN
K743 LTCSTKVKTLLLNAA
S812 DTYRKTPSPSGSLLR
K855 KGPETRDKLPStEVK
S858 ETRDKLPStEVKEDs
T859-p TRDKLPStEVKEDsA
K862 KLPStEVKEDsAYGS
S865-p StEVKEDsAYGSQSV
Y867 EVKEDsAYGSQSVEQ
S869 KEDsAYGSQSVEQEA
S871 DsAYGSQSVEQEAEK
4810 : Phospho-NF-KappaB2 p100 (Ser866/870) Antibody
4810 : Phospho-NF-KappaB2 p100 (Ser866/870) Antibody
  rat

 
D10 RCYDPGLDGIPEYDD
S23 DDFEFSPSIVEPKDP
Y55 QRGFRFRYGCEGPSH
K72 LPGASSEKGRKTYPT
K90 CNYEGPAKIEVDLVT
T97 KIEVDLVTHSDPPRA
S99 EVDLVTHSDPPRAHA
S108 PPRAHAHSLVGKQCS
S115 SLVGKQCSELGVCAV
S123 ELGVCAVSVGPKDMT
S161 LQRQRLRSKPQGLTE
S195 SIVRLRFSAFLRASD
S222 QPIHDSKSPGASNLK
S277 WQAFGDFSPTDVHKQ
Y285 PTDVHKQYAIVFRTP
K298 TPPYHKMKIERPVTV
K321 GGDVSDSKQFTYYPL
Y325 SDSKQFTYYPLVEDK
Y326 DSKQFTYYPLVEDKE
- gap
A424 EAEEQPRAPSEAPQS
A428 QPRAPSEAPQSEQQA
T438 SEQQALDTLQRAREY
T650 MEELGLVTHLVTKLH
T654 GLVTHLVTKLHANVN
T681 AAGLGSPTLTRLLLK
K688 TLTRLLLKAGADIHA
S706 EPLCPLPSPPTSGSD
S710 PLPSPPTSGSDSDSE
S712 PSPPTSGSDSDSEGP
S714 PPTSGSDSDSEGPET
S716 TSGSDSDSEGPETDT
N726 PETDTQRNFRGHTPL
K740 LDLTRSTKVKTLLLN
K742 LTRSTKVKTLLLNAA
S811 DTYRKTPSPSGSLLR
K854 KAPETRDKLPSTEVK
S857 ETRDKLPSTEVKEDs
T858 TRDKLPSTEVKEDsA
K861 KLPSTEVKEDsAYGs
S864-p STEVKEDsAYGsQSV
Y866 EVKEDsAYGsQSVEQ
S868-p KEDsAYGsQSVEQEA
S870 DsAYGsQSVEQEAEK
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