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Protein Page:
HNF4 alpha (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
HNF4 alpha a nuclear transcription factor which binds DNA as a homodimer. Controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic gene, alpha 1-antitrypsin, apolipoprotein CIII, and transthyretin genes. May be essential for development of the liver, kidney and intestine. Mutations have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Four alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; Nuclear receptor; DNA binding protein
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; ligand-dependent nuclear receptor activity; protein binding; protein homodimerization activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; fatty acid binding; transcription factor activity; receptor binding
Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; regulation of lipid metabolic process; positive regulation of transcription, DNA-dependent; lipid homeostasis; glucose homeostasis; endocrine pancreas development; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; regulation of gastrulation; xenobiotic metabolic process; response to glucose stimulus; ornithine metabolic process; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; lipid metabolic process; blood coagulation; regulation of insulin secretion; sex differentiation; phospholipid homeostasis
Reference #:  P41235 (UniProtKB)
Alt. Names/Synonyms: FLJ39654; hepatic nuclear factor 4 alpha; hepatocyte nuclear factor 4, alpha; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; HNF4; HNF4A; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; HNF4alpha10/11/12; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; TCF; TCF-14; TCF14; Transcription factor 14; Transcription factor HNF-4; transcription factor-14
Gene Symbols: HNF4A
Molecular weight: 52,785 Da
Basal Isoelectric point: 7.15  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  AMPK Signaling  |  Wnt/ß-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

HNF4 alpha

Protein Structure Not Found.


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Sites Implicated In
transcription, induced: S167‑p
transcription, inhibited: Y286‑p, Y288‑p, S313‑p
intracellular localization: Y286‑p, Y288‑p
molecular association, regulation: S167‑p
phosphorylation: Y286‑p, Y288‑p
protein degradation: Y286‑p, Y288‑p, S313‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 1 Y23-p SAALDPAyTTLEFEN
1 0 S87 CKGFFRRSVRKNHMY
0 1 S99-p HMYSCRFsRQCVVDk
1 1 K106-ac sRQCVVDkDkRNQCR
1 0 K108-ac QCVVDkDkRNQCRYC
1 0 K126-ac KCFRAGMkkEAVQNE
1 0 K127-ac CFRAGMkkEAVQNER
1 0 S138-p QNERDRIsTRRssyE
0 2 T139 NERDRIsTRRssyED
3 7 S142-p DRIsTRRssyEDsSL
2 13 S143-p RIsTRRssyEDsSLP
0 1 Y144-p IsTRRssyEDsSLPs
1 0 S147-p RRssyEDsSLPsINA
0 1 S148 RssyEDsSLPsINAL
1 1 S151-p yEDsSLPsINALLQA
1 1 T166-p EVLSRQItsPVsGIN
3 29 S167-p VLSRQItsPVsGING
0 1 S170-p RQItsPVsGINGDIR
0 1 K180 NGDIRAKKIASIADV
1 1 S190-p SIADVCEsMKEQLLV
1 1 K234-ub HLLLGATkRSMVFKD
1 1 Y286-p LQIDDNEyAyLKAII
1 1 Y288-p IDDNEyAyLKAIIFF
0 1 K300 IFFDPDAKGLsDPGk
1 0 S303-p DPDAKGLsDPGkIkR
1 1 K307-ub KGLsDPGkIkRLRsQ
0 1 K309-ub LsDPGkIkRLRsQVQ
3 1 S313-p GkIkRLRsQVQVsLE
1 0 S318-p LRsQVQVsLEDYIND
1 0 S378-p QEMLLGGsPSDAPHA
0 4 T429-p RPRGQAAtPEtPQPs
1 4 T432-p GQAAtPEtPQPsPPG
2 3 S436-p tPEtPQPsPPGGsGs
0 1 S441-p QPsPPGGsGsEPYKL
1 0 S443-p sPPGGsGsEPYKLLP
1 0 T455-p LLPGAVAtIVkPLSA
1 0 K458-ac GAVAtIVkPLSAIPQ
1 0 T469-p AIPQPTItKQEVI__
  mouse

 
Y23 SAALDPAYTTLEFEN
S87 CKGFFRRSVRKNHMY
S99 HMYSCRFSRQCVVDK
K106 SRQCVVDKDKRNQCR
K108 QCVVDKDKRNQCRYC
K126 KCFRAGMKKEAVQNE
K127 CFRAGMKKEAVQNER
S138 QNERDRIStRRssYE
T139-p NERDRIStRRssYED
S142-p DRIStRRssYEDSsL
S143-p RIStRRssYEDSsLP
Y144 IStRRssYEDSsLPS
S147 RRssYEDSsLPSINA
S148-p RssYEDSsLPSINAL
S151 YEDSsLPSINALLQA
T166 EVLSQQITsPISGIN
S167-p VLSQQITsPISGING
S170 QQITsPISGINGDIR
K180-ub NGDIRAKkIANITDV
S190 NITDVCESMKEQLLV
K234-ub HLLLGATkRSMVFKD
Y286 LQIDDNEYACLKAII
C288 IDDNEYACLKAIIFF
K300-ub IFFDPDAkGLSDPGK
S303 DPDAkGLSDPGKIKR
K307 kGLSDPGKIKRLRSQ
K309 LSDPGKIKRLRSQVQ
S313 GKIKRLRSQVQVSLE
S318 LRSQVQVSLEDYIND
S378 QEMLLGGSASDAPHT
T429-p RPRGQAAtPEtPQPs
T432-p GQAAtPEtPQPsPPS
S436-p tPEtPQPsPPSGSGS
S441 QPsPPSGSGSESYKL
S443 sPPSGSGSESYKLLP
T455 LLPGAITTIVKPPSA
K458 GAITTIVKPPSAIPQ
T469 AIPQPTITKQEAI__
  rat

 
Y23 SAALDPAYTTLEFEN
S87-p CKGFFRRsVRKNHMY
S99 HMYSCRFSRQCVVDK
K106 SRQCVVDKDKRNQCR
K108 QCVVDKDKRNQCRYC
K126 KCFRAGMKKEAVQNE
K127 CFRAGMKKEAVQNER
S138 QNERDRISTRRssYE
T139 NERDRISTRRssYED
S142-p DRISTRRssYEDSSL
S143-p RISTRRssYEDSSLP
Y144 ISTRRssYEDSSLPS
S147 RRssYEDSSLPSINA
S148 RssYEDSSLPSINAL
S151 YEDSSLPSINALLQA
T166 EVLSQQITSPISGIN
S167 VLSQQITSPISGING
S170 QQITSPISGINGDIR
K180 NGDIRAKKIANITDV
S190 NITDVCESMKEQLLV
K234 HLLLGATKRSMVFKD
Y286 LQIDDNEYACLKAII
C288 IDDNEYACLKAIIFF
K300 IFFDPDAKGLSDPGK
S303 DPDAKGLSDPGKIKR
K307 KGLSDPGKIKRLRSQ
K309 LSDPGKIKRLRSQVQ
S313 GKIKRLRSQVQVSLE
S318 LRSQVQVSLEDYIND
S378 QEMLLGGSASDAPHA
T429 RPRGQAATPETPQPs
T432 GQAATPETPQPsPPS
S436-p TPETPQPsPPSGSGS
S441 QPsPPSGSGSESYKL
S443 sPPSGSGSESYKLLP
T455 LLPGAITTIVKPPSA
K458 GAITTIVKPPSAIPQ
T469 AIPQPTITKQEAI__
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