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Protein Page:
NuMA-1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
NuMA-1 a coiled-coil nuclear protein that is required to establish and maintain mammalian spindle poles. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. Two human splice-variant isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; Cell cycle regulation
Cellular Component: spindle pole; nuclear matrix; dendrite; chromosome; cytosol; Golgi membrane; nucleoplasm; cell soma; apical part of cell; spindle microtubule; cytoplasm; nucleolus; spindle; nucleus
Molecular Function: protein binding; microtubule binding; structural molecule activity
Biological Process: mitosis; meiotic cell cycle; establishment of mitotic spindle orientation; mitotic cell cycle; mitotic anaphase; G2/M transition of mitotic cell cycle; nuclear organization and biogenesis
Reference #:  Q14980 (UniProtKB)
Alt. Names/Synonyms: centrophilin stabilizes mitotic spindle in mitotic cells; Nuclear mitotic apparatus protein 1; NUMA; NuMA protein; NUMA1; SP-H antigen; structural nuclear protein
Gene Symbols: NUMA1
Molecular weight: 238,260 Da
Basal Isoelectric point: 5.63  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NuMA-1

Protein Structure Not Found.


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Sites Implicated In
cytoskeletal reorganization: T2055‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 T2-p ______MtLHAtRGA
0 2 T6-p __MtLHAtRGAALLS
0 1 T46-p IIDRIHGtEEGQQIL
0 1 K54-u EEGQQILkQPVSERL
0 12 S76-p QKNRKHPssPECLVS
0 49 S77-p KNRKHPssPECLVSA
0 1 S112-p HSTMSSKsPRDWEQF
0 22 S169-p STFPEELsPPsHQAK
0 5 S172-p PEELsPPsHQAKREI
0 2 S200-p GNNFLSGsPAsPMGD
0 1 A202 NFLSGsPAsPMGDIL
0 11 S203-p FLSGsPAsPMGDILQ
0 9 T211-p PMGDILQtPQFQMRR
0 1 K247 NRKLLTEKDAQIAMM
0 1 K267-u RLALLNEkQAAsPLE
0 6 S271-p LNEkQAAsPLEPKEL
0 1 K326-u ENGDLSFkLREFASH
0 1 K358-u ATQEWLEkQAQLEkE
0 1 K364-u EkQAQLEkELSAALQ
0 20 K379-a DKKCLEEkNEILQGk
0 2 K386-u kNEILQGkLsQLEEH
0 6 S388-p EILQGkLsQLEEHLs
0 34 S395-p sQLEEHLsQLQDNPP
0 15 N400 HLsQLQDNPPQEKGE
0 2 K561-u EQLSSSLkQKEQQLK
0 2 S595-p AAEEREAsLRERDAA
0 1 K604-u RERDAALkQLEALEK
0 1 K616-u LEKEKAAkLEILQQQ
0 3 S820-p WRERYEDsQQEEAQY
0 1 K855-u ELQEAKEkVAGIESH
0 1 K891-a ALQQVQEkEVRAQKL
0 13 K891-u ALQQVQEkEVRAQKL
0 2 K908-u DLSTLQEkMAATSKE
0 2 K939-u TASRELVkEPARAGD
0 1 P949 ARAGDRQPEWLEEQQ
0 1 T963-p QGRQFCStQAALQAM
0 4 S991-p LRAALMEsQGQQQEE
0 2 S1109-p ASGSGAQsEAAGRTE
0 3 K1121-a RTEPTGPkLEALRAE
0 1 S1149-p QADSLERsLEAERAS
0 1 S1187-p HSQSALAsAQRELAA
0 1 N1224 RQEAERKNsLIssLE
0 22 S1225-p QEAERKNsLIssLEE
0 1 S1228-p ERKNsLIssLEEEVS
0 1 S1229-p RKNsLIssLEEEVSI
0 3 S1262-p VMAESEKsQKLEERL
0 2 K1330-u EELGQELkAWQEkFF
0 1 K1335-u ELkAWQEkFFQKEQA
0 1 K1430-u AQQLRAEkASYAEQL
0 2 K1475-u ELDQAREkYVQELAA
0 1 T1489-p AVRADAEtRLAEVQR
0 1 K1511-a ELEVMTAkYEGAKVK
0 1 K1511-u ELEVMTAkYEGAKVK
0 3 K1573-u KVQQQKLkAVQAQGG
0 2 S1582-p VQAQGGEsQQEAQRL
0 2 S1601-p NELQAQLsQKEQAAE
0 9 K1624-a AKTHYDAkKQQNQEL
0 1 K1699-u QQLRDLGkFQVATDA
0 4 K1708-u QVATDALkSREPQAK
0 3 S1721-p AKPQLDLsIDsLDLs
0 3 S1724-p QLDLsIDsLDLsCEE
0 2 S1728-p sIDsLDLsCEEGtPL
0 1 T1733-p DLsCEEGtPLSITSK
0 13 T1744-p ITSKLPRtQPDGtsV
0 6 T1749-p PRtQPDGtsVPGEPA
0 3 S1750-p RtQPDGtsVPGEPAs
0 113 S1757-p sVPGEPAsPIsQRLP
0 14 S1760-p GEPAsPIsQRLPPkV
1 1 K1766-s IsQRLPPkVEsLEsL
0 1 K1766-u IsQRLPPkVEsLEsL
0 34 S1769-p RLPPkVEsLEsLyFt
0 32 S1772-p PkVEsLEsLyFtPIP
1 91 Y1774-p VEsLEsLyFtPIPAR
1 40 T1776-p sLEsLyFtPIPARSQ
0 6 S1788-p RSQAPLEssLDsLGD
0 9 S1789-p SQAPLEssLDsLGDV
0 9 S1792-p PLEssLDsLGDVFLD
0 14 S1800-p LGDVFLDsGRKtRsA
1 2 T1804-p FLDsGRKtRsARRRt
0 4 S1806-p DsGRKtRsARRRttQ
1 192 T1811-p tRsARRRttQIINIt
1 106 T1812-p RsARRRttQIINItM
0 2 T1818-p ttQIINItMTKKLDV
0 1 D1829-ca KLDVEEPdsANssFy
0 17 S1830-p LDVEEPdsANssFys
0 3 S1833-p EEPdsANssFystRs
0 17 S1834-p EPdsANssFystRsA
0 43 Y1836-p dsANssFystRsAPA
0 13 S1837-p sANssFystRsAPAs
0 3 T1838-p ANssFystRsAPAsQ
0 18 S1840-p ssFystRsAPAsQAs
0 14 S1844-p stRsAPAsQAsLRAT
0 1 S1844-gl stRsAPAsQAsLRAT
0 20 S1847-p sAPAsQAsLRATsst
0 3 S1852-p QAsLRATsstQSLAR
0 11 S1853-p AsLRATsstQSLARL
0 3 T1854-p sLRATsstQSLARLG
0 2 S1856 RATsstQSLARLGsP
0 33 S1862-p QSLARLGsPDyGNSA
0 4 Y1865-p ARLGsPDyGNSALLs
0 1 S1868 GsPDyGNSALLsLPG
0 3 S1872-p yGNSALLsLPGYRPt
0 2 T1879-p sLPGYRPtTRssARR
1 0 S1882-p GYRPtTRssARRsQA
1 0 S1883-p YRPtTRssARRsQAG
1 12 S1887-p TRssARRsQAGVSSG
0 5 G1890 sARRsQAGVSSGAPP
0 2 S1901-p GAPPGRNsFYMGTCQ
0 1 T1906 RNsFYMGTCQDEPEQ
0 3 S1942-p KTCYPLEsRPsLsLG
0 16 S1945-p YPLEsRPsLsLGtIt
0 8 S1947-p LEsRPsLsLGtItDE
0 6 T1950-p RPsLsLGtItDEEMK
0 12 T1952-p sLsLGtItDEEMKTG
0 47 S1969-p QETLRRAsMQPIQIA
1 43 S1991-p RQQRKRVsLEPHQGP
0 11 L1992 QQRKRVsLEPHQGPG
0 5 P1994 RKRVsLEPHQGPGtP
0 63 T2000-p EPHQGPGtPEskKAT
0 3 S2003-p QGPGtPEskKATSCF
0 4 K2004-a GPGtPEskKATSCFP
1 33 T2015-p SCFPRPMtPRDRHEG
0 23 S2026-p RHEGRKQstTEAQKK
0 2 T2027-p HEGRKQstTEAQKKA
1 8 S2047-p KQADRRQsMAFsILN
0 2 S2051-p RRQsMAFsILNtPkK
2 26 T2055-p MAFsILNtPkKLGNs
0 2 K2057-a FsILNtPkKLGNsLL
0 1 K2058 sILNtPkKLGNsLLR
0 4 S2062-p tPkKLGNsLLRRGAS
0 2 K2070-a LLRRGASkkALsKAs
0 2 K2071-a LRRGASkkALsKAsP
0 1 S2074-p GASkkALsKAsPNtR
0 27 S2077-p kkALsKAsPNtRSGt
0 1 T2080-p LsKAsPNtRSGtRRs
1 0 T2084-p sPNtRSGtRRsPRIA
2 0 S2087-p tRSGtRRsPRIATTT
0 1 T2093 RsPRIATTTASAATA
1 18 T2106-p TAAAIGAtPRAKGKA
3429 : Phospho-NuMA (Ser395) Antibody
  mouse

 
T2 ______MTLHATRAA
T6 __MTLHATRAATLLS
T46 IINTIHDTKEGQQIL
Q54 KEGQQILQQPLPERL
S76 QKNRKHPSSTQCLVS
S77 KNRKHPSSTQCLVSV
N112 QSTMSSRNLRDWEQF
S167-p STLSEELsPPSHQTK
S170 SEELsPPSHQTKRKI
S198-p ENNFLSGsPssPMGD
S200-p NFLSGsPssPMGDIL
S201-p FLSGsPssPMGDILQ
T209 PMGDILQTPQFQMRR
K245-u SLKLLTEkDAQIAMM
K265 HLALLNEKQAAsSQE
S269-p LNEKQAAsSQEPSEL
K324 ENGDLSFKVREFANH
K356 ASQEWAEKQARLENE
N362 EKQARLENELSTALQ
K377 DKKCLEEKNEILQGK
K384 KNEILQGKLSQLEDQ
S386 EILQGKLSQLEDQAT
T393 SQLEDQATRLQEsPA
S398-p QATRLQEsPAPEKGE
K555 EQLSSSLKLKEQQLE
S589-p VAEAREAsLRERDTA
Q598 RERDTARQQLETVEK
K610 VEKEKDAKLESLQQQ
R814 RQKRFEARQQEEARY
- gap
K878 AIQQVQEKEVRAQKL
K878 AIQQVQEKEVRAQKL
K895 DLSALQEKMAATNKE
K926 TASLELLKEPPRAAN
S936 PRAANRASDQLGEQQ
T950 QGRPFSSTHAAVKAM
S978 LRAALIKSQGQQQEE
S1105 ASGPGTQSETAGKTD
E1117 KTDAPGPELQALRAE
S1145 QVEGLTHSLKSERAC
S1183 HNQAASASAQRELQA
S1220-p QQEAERKssLIssLE
S1221-p QEAERKssLIssLEE
S1224-p ERKssLIssLEEEVS
S1225-p RKssLIssLEEEVSI
S1258 VVAESEKSQKLEERL
K1326 EELGQELKAWQEKFF
K1331 ELKAWQEKFFQKEQA
K1426 AQQLRAEKASYAEQL
K1471-u ELDQAREkYVQELAA
T1485 AVRTDAETHLAEMRQ
K1507 ELEVMTAKYEGAKVK
K1507 ELEVMTAKYEGAKVK
K1555 KVQQQKLKAFQAQRG
S1564 FQAQRGESQQEVQRL
S1583 NELQAQLSQKEQAAE
K1606 AKTHYDAKKQQNQKL
K1681 QQLRDLGKFQVATDA
K1690 QVATDALKSREPQVK
S1703-p VKPQLDLsIDsLDLs
S1706-p QLDLsIDsLDLsLEE
S1710-p sIDsLDLsLEEGTPC
T1715 DLsLEEGTPCSVASK
T1726-p VASKLPRtQPDGTsV
T1731 PRtQPDGTsVPGEPA
S1732-p RtQPDGTsVPGEPAs
S1739-p sVPGEPAsPIsQRLP
S1742-p GEPAsPIsQRLPPKV
K1748 IsQRLPPKVEsLEsL
K1748 IsQRLPPKVEsLEsL
S1751-p RLPPKVEsLEsLYFt
S1754-p PKVEsLEsLYFtPTP
Y1756 VEsLEsLYFtPTPAR
T1758-p sLEsLYFtPTPARGQ
T1770-p RGQAPLEtsLDsLGD
S1771-p GQAPLEtsLDsLGDA
S1774-p PLEtsLDsLGDAFPD
S1782-p LGDAFPDsGRKTRSA
T1786 FPDsGRKTRSARRRt
S1788 DsGRKTRSARRRttQ
T1793-p TRSARRRttQIINIT
T1794-p RSARRRttQIINITM
T1800 ttQIINITMTKKLEL
D1811 KLELEEPDSANSSFY
S1812 LELEEPDSANSSFYS
S1815 EEPDSANSSFYSTQS
S1816 EPDSANSSFYSTQSA
Y1818 DSANSSFYSTQSAPA
S1819 SANSSFYSTQSAPAs
T1820 ANSSFYSTQSAPAsQ
S1822 SSFYSTQSAPAsQAN
S1826-p STQSAPAsQANLRAT
S1826 STQSAPASQANLRAT
N1829 SAPAsQANLRATSsT
S1834 QANLRATSsTQsLAR
S1835-p ANLRATSsTQsLARL
T1836 NLRATSsTQsLARLG
S1838-p RATSsTQsLARLGsP
S1844-p QsLARLGsPDDGNsA
D1847 ARLGsPDDGNsALLS
S1850-p GsPDDGNsALLSLPG
S1854 DGNsALLSLPGYRPT
T1861 SLPGYRPTTRSSARR
S1864 GYRPTTRSSARRSQA
S1865 YRPTTRSSARRSQAr
S1869 TRSSARRSQArMSSG
R1872-m1 SARRSQArMSSGAPQ
S1883 GAPQGRNSFYMGTCQ
T1888 RNSFYMGTCQDEPEQ
S1924 KTCYPLESRPTLSLA
T1927 YPLESRPTLSLATIT
S1929 LESRPTLSLATITDE
T1932 RPTLSLATITDEEMK
T1934 TLSLATITDEEMKTG
S1951-p RETLRRAsMQPAQIA
S1973-p RQQRKRVssEtHQGP
S1974-p QQRKRVssEtHQGPG
T1976-p RKRVssEtHQGPGtP
T1982-p EtHQGPGtPEsKKAT
S1985-p QGPGtPEsKKATSCF
K1986 GPGtPEsKKATSCFP
T1997-p SCFPRPMtPRDRHEG
S2008 RHEGRKQSSTADTQK
S2009 HEGRKQSSTADTQKK
S2029-p KQADRRQsMAFSILN
S2033 RRQsMAFSILNtPKk
T2037-p MAFSILNtPKkLGNs
K2039 FSILNtPKkLGNsLL
K2040-u SILNtPKkLGNsLLR
S2044-p tPKkLGNsLLRRGAS
K2052 LLRRGASKKTPAKVs
K2053 LRRGASKKTPAKVsP
A2056 GASKKTPAKVsPNPR
S2059-p KKTPAKVsPNPRSGT
P2062 PAKVsPNPRSGTRRS
T2066 sPNPRSGTRRSPRIA
S2069 PRSGTRRSPRIATtT
T2075-p RSPRIATtTTGTATV
T2085-p GTATVATtPRAKGKV
3429 : Phospho-NuMA (Ser395) Antibody
  rat

 
- gap
- gap
T32 IINSIHDTKEGQQIL
Q40 KEGQQILQQPLPERL
S62 QKNRKHPSSTQCLVS
S63 KNRKHPSSTQCLVSV
N98 HSTMSSRNPRDWEQF
S153-p STLSEELsPPsHQAK
S156-p SEELsPPsHQAKRKI
S184 ENNFLSGSPSSPVGD
S186 NFLSGSPSSPVGDIL
S187 FLSGSPSSPVGDILQ
T195 PVGDILQTPQFQMRR
K231 SLKLLTEKDAQIAMM
K251 HLALLNEKQAAsPQE
S255-p LNEKQAAsPQEPSEL
K310 ENGDLSFKVREFANH
K342 ASQEWAEKQAHLESE
S348 EKQAHLESELSTALQ
K363 DKKCLEEKSEILQEK
K370 KSEILQEKISQLEDR
S372 EILQEKISQLEDRAA
A379 SQLEDRAAQLQGsPA
S384-p RAAQLQGsPAPEKGE
K541 EQLSSSLKLKEQQLE
S575 IVEAREASVRERDAA
Q584 RERDAARQQLETLEK
K596 LEKEKDAKLESLQQQ
R800 WQKRVEARQQEEARY
- gap
K866 ALQQVQEKEARAQKL
K866 ALQQVQEKEARAQKL
K883 DLSALREKMAATNKE
K913 AAASHELKEPPRAGN
S923-p PRAGNQEsDWEEEQA
T936 QARPLGSTQAALKAV
S964 LRAALMQSQGQQQEV
L1089 GDGPGSQLHTPGKTE
E1101 KTEAPGPEVEALRAE
S1129 QVEGLTHSLESERAC
S1167 HNQAASASAQRELQA
S1204 QQEAERKSSLISSLE
S1205 QEAERKSSLISSLEE
S1208 ERKSSLISSLEEEVS
S1209 RKSSLISSLEEEVSI
S1242 VVAESEKSQKLEERL
K1310 EESGQELKAWQEKFF
K1315 ELKAWQEKFFQKEQA
K1410 AQQLRAEKASYAEQL
K1455 ELDQAREKYVQELAA
T1469 AVRTDAETHLAEMRQ
K1491 ELEVMTAKYESAKVK
K1491 ELEVMTAKYESAKVK
K1539 RVQQQKLKAFQAQGG
S1548 FQAQGGESQQEVQCL
S1567 SELQAQLSQKEQAAE
K1590 AKTHYDAKKQQNQEL
K1665 QQLRDLGKFQVATDA
K1674 QVATDALKSREPQVK
S1687 VKPQLDLSIDSLDLS
S1690 QLDLSIDSLDLSLEE
S1694 SIDSLDLSLEEGTPC
T1699 DLSLEEGTPCSVASK
T1710 VASKLPRTQPDGTSV
T1715 PRTQPDGTSVPGEPA
S1716 RTQPDGTSVPGEPAs
S1723-p SVPGEPAsPISQRLP
S1726 GEPAsPISQRLPPKV
K1732 ISQRLPPKVESLESL
K1732 ISQRLPPKVESLESL
S1735 RLPPKVESLESLYFT
S1738 PKVESLESLYFTPIP
Y1740 VESLESLYFTPIPAR
T1742 SLESLYFTPIPARGQ
T1754 RGQAPLETSLDSLGD
S1755 GQAPLETSLDSLGDA
S1758 PLETSLDSLGDAFPD
S1766 LGDAFPDSGRKTRSA
T1770 FPDSGRKTRSARRRt
S1772 DSGRKTRSARRRtTQ
T1777-p TRSARRRtTQIINIT
T1778 RSARRRtTQIINITM
T1784 tTQIINITMTKKLEL
D1795 KLELEEPDSANSSFY
S1796 LELEEPDSANSSFYS
S1799 EEPDSANSSFYSTQS
S1800 EPDSANSSFYSTQSA
Y1802 DSANSSFYSTQSAPA
S1803 SANSSFYSTQSAPAS
T1804 ANSSFYSTQSAPASQ
S1806 SSFYSTQSAPASQAN
S1810 STQSAPASQANLRAT
S1810 STQSAPASQANLRAT
N1813 SAPASQANLRATSST
S1818 QANLRATSSTQSLAR
S1819 ANLRATSSTQSLARL
T1820 NLRATSSTQSLARLG
S1822 RATSSTQSLARLGsP
S1828-p QSLARLGsPDDSNSA
D1831 ARLGsPDDSNSALLS
S1834 GsPDDSNSALLSLPG
S1838 DSNSALLSLPGYRPT
T1845 SLPGYRPTTRSSARR
S1848 GYRPTTRSSARRSQT
S1849 YRPTTRSSARRSQTR
S1853 TRSSARRSQTRTSSG
R1856 SARRSQTRTSSGAPH
S1867 GAPHGRNSFYMGtCQ
T1872-p RNSFYMGtCQDEPEQ
S1908 KTCYPLESRPALSLA
A1911 YPLESRPALSLATIT
S1913 LESRPALSLATITDE
T1916 RPALSLATITDEEMK
T1918 ALSLATITDEEMKTG
S1935-p RETLRRAsMQPAQIA
S1957-p RQQRKRVssETHQGP
S1958-p QQRKRVssETHQGPG
T1960 RKRVssETHQGPGtP
T1966-p ETHQGPGtPESKKAT
S1969 QGPGtPESKKATSCF
K1970 GPGtPESKKATSCFP
T1981 SCFPRPMTPRDRHEG
N1992 RHEGRKQNSTADAQK
S1993 HEGRKQNSTADAQKK
S2013 KQSDRRQSMAFSILN
S2017 RRQSMAFSILNtPKK
T2021-p MAFSILNtPKKLGNS
K2023 FSILNtPKKLGNSLL
K2024 SILNtPKKLGNSLLR
S2028 tPKKLGNSLLRRGTS
K2036 LLRRGTSKKTPAKVs
K2037 LRRGTSKKTPAKVsP
A2040 GTSKKTPAKVsPNPR
S2043-p KKTPAKVsPNPRSGT
P2046 PAKVsPNPRSGTRRS
T2050 sPNPRSGTRRSPRIA
S2053 PRSGTRRSPRIATTT
T2059 RSPRIATTTAGAATA
T2069-p GAATAATtPRAKGKA
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