Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
NuMA-1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
NuMA-1 a coiled-coil nuclear protein that is required to establish and maintain mammalian spindle poles. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. Two human splice-variant isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Cell cycle regulation; Motility/polarity/chemotaxis
Cellular Component: nucleoplasm; spindle pole; nuclear matrix; spindle microtubule; apical part of cell; cytoplasm; nucleolus; spindle; chromosome; nucleus; cytosol
Molecular Function: protein binding; structural molecule activity
Biological Process: establishment of mitotic spindle orientation; cell division; mitotic cell cycle; G2/M transition of mitotic cell cycle; mitotic anaphase; nuclear organization and biogenesis
Reference #:  Q14980 (UniProtKB)
Alt. Names/Synonyms: centrophilin stabilizes mitotic spindle in mitotic cells; Nuclear mitotic apparatus protein 1; NUMA; NuMA protein; NUMA1; SP-H antigen; structural nuclear protein
Gene Symbols: NUMA1
Molecular weight: 238,260 Da
Basal Isoelectric point: 5.63  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NuMA-1
Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  DISEASE  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

 
0 1 T2-p ______MtLHAtRGA
0 2 T6-p __MtLHAtRGAALLS
0 1 T46-p IIDRIHGtEEGQQIL
0 1 K54-u EEGQQILkQPVSERL
0 11 S76-p QKNRKHPssPECLVS
0 48 S77-p KNRKHPssPECLVSA
0 1 S91-p AQKVLEGsELELAKM
0 1 T107-p MLLLYHStMSSKsPR
0 1 S112-p HStMSSKsPRDWEQF
0 20 S169-p STFPEELsPPsHQAK
0 4 S172-p PEELsPPsHQAKREI
0 2 S200-p GNNFLSGsPAsPMGD
0 1 A202 NFLSGsPAsPMGDIL
0 11 S203-p FLSGsPAsPMGDILQ
0 9 T211-p PMGDILQtPQFQMRR
0 1 K247 NRKLLTEKDAQIAMM
0 1 K267-u RLALLNEkQAAsPLE
0 4 S271-p LNEkQAAsPLEPKEL
0 1 R311-m2 TEKSQMDrKINQLSE
0 1 K326-u ENGDLSFkLREFASH
0 1 K358-u ATQEWLEkQAQLEkE
0 1 K364-u EkQAQLEkELSAALQ
0 20 K379-a DKKCLEEkNEILQGk
0 2 K386-u kNEILQGkLsQLEEH
0 6 S388-p EILQGkLsQLEEHLs
0 34 S395-p sQLEEHLsQLQDNPP
0 12 N400 HLsQLQDNPPQEKGE
0 1 E530 GLKQQAKEKQAQLAQ
0 2 K561-u EQLSSSLkQKEQQLK
0 2 S595-p AAEEREAsLRERDAA
0 1 K604-u RERDAALkQLEALEK
0 1 K616-u LEKEKAAkLEILQQQ
0 1 T636-p EARDSAQtsVTQAQR
0 1 S637-p ARDSAQtsVTQAQRE
0 3 S820-p WRERYEDsQQEEAQY
0 1 K855-u ELQEAKEkVAGIESH
0 1 K891-a ALQQVQEkEVRAQKL
0 13 K891-u ALQQVQEkEVRAQKL
0 2 K908-u DLSTLQEkMAATSKE
0 2 K939-u TASRELVkEPARAGD
0 1 T963-p QGRQFCStQAALQAM
0 4 S991-p LRAALMEsQGQQQEE
0 1 S1103-p KKEKEHAsGSGAQsE
0 2 S1109-p AsGSGAQsEAAGRTE
0 3 K1121-a RTEPTGPkLEALRAE
0 1 S1145-p KQQEQADsLERSLEA
0 1 S1187-p HSQSALAsAQRELAA
0 1 N1224 RQEAERKNsLIssLE
0 21 S1225-p QEAERKNsLIssLEE
0 1 S1228-p ERKNsLIssLEEEVS
0 1 S1229-p RKNsLIssLEEEVSI
0 3 S1262-p VMAESEKsQKLEERL
0 1 S1278-p LLQAETAsNsARAAE
0 1 S1280-p QAETAsNsARAAERS
0 2 K1330-u EELGQELkAWQEkFF
0 1 K1335-u ELkAWQEkFFQKEQA
0 1 K1430-u AQQLRAEkASYAEQL
0 2 K1475-u ELDQAREkYVQELAA
0 1 T1489-p AVRADAEtRLAEVQR
0 1 K1511-a ELEVMTAkYEGAKVK
0 1 K1511-u ELEVMTAkYEGAKVK
0 3 K1573-u KVQQQKLkAVQAQGG
0 2 S1582-p VQAQGGEsQQEAQRL
0 2 S1601-p NELQAQLsQKEQAAE
0 9 K1624-a AKTHYDAkKQQNQEL
0 1 K1699-u QQLRDLGkFQVATDA
0 4 K1708-u QVATDALkSREPQAK
0 3 S1721-p AKPQLDLsIDsLDLs
0 3 S1724-p QLDLsIDsLDLsCEE
0 2 S1728-p sIDsLDLsCEEGtPL
0 1 T1733-p DLsCEEGtPLSITSK
0 13 T1744-p ITSKLPRtQPDGtsV
0 6 T1749-p PRtQPDGtsVPGEPA
0 3 S1750-p RtQPDGtsVPGEPAs
0 107 S1757-p sVPGEPAsPIsQRLP
0 11 S1760-p GEPAsPIsQRLPPkV
0 1 K1766-a IsQRLPPkVEsLEsL
0 1 K1766-s IsQRLPPkVEsLEsL
0 1 K1766-u IsQRLPPkVEsLEsL
0 31 S1769-p RLPPkVEsLEsLyFt
0 31 S1772-p PkVEsLEsLyFtPIP
1 91 Y1774-p VEsLEsLyFtPIPAR
1 39 T1776-p sLEsLyFtPIPARSQ
0 6 S1788-p RSQAPLEssLDsLGD
0 9 S1789-p SQAPLEssLDsLGDV
0 9 S1792-p PLEssLDsLGDVFLD
0 14 S1800-p LGDVFLDsGRKtRsA
0 2 T1804-p FLDsGRKtRsARRRt
0 4 S1806-p DsGRKtRsARRRttQ
0 192 T1811-p tRsARRRttQIINIt
0 106 T1812-p RsARRRttQIINItM
0 2 T1818-p ttQIINItMtKKLDV
0 1 T1820-p QIINItMtKKLDVEE
0 1 D1829-ca KLDVEEPdsANssFy
0 15 S1830-p LDVEEPdsANssFys
0 3 S1833-p EEPdsANssFystRs
0 16 S1834-p EPdsANssFystRsA
0 41 Y1836-p dsANssFystRsAPA
0 13 S1837-p sANssFystRsAPAs
0 3 T1838-p ANssFystRsAPAsQ
0 17 S1840-p ssFystRsAPAsQAs
0 13 S1844-p stRsAPAsQAsLRAT
0 1 S1844-g stRsAPAsQAsLRAT
0 20 S1847-p sAPAsQAsLRATsst
0 3 S1852-p QAsLRATsstQSLAR
0 11 S1853-p AsLRATsstQSLARL
0 3 T1854-p sLRATsstQSLARLG
0 1 S1856 RATsstQSLARLGsP
0 31 S1862-p QSLARLGsPDyGNSA
0 4 Y1865-p ARLGsPDyGNSALLs
0 1 S1868 GsPDyGNSALLsLPG
0 3 S1872-p yGNSALLsLPGYRPt
0 2 T1879-p sLPGYRPtTRSSARR
0 11 S1887-p TRSSARRsQAGVsSG
0 5 G1890 SARRsQAGVsSGAPP
0 1 S1892-p RRsQAGVsSGAPPGR
0 2 S1901-p GAPPGRNsFyMGTCQ
0 1 Y1903-p PPGRNsFyMGTCQDE
0 3 S1942-p KTCYPLEsRPsLsLG
0 15 S1945-p YPLEsRPsLsLGtIt
0 7 S1947-p LEsRPsLsLGtItDE
0 6 T1950-p RPsLsLGtItDEEMK
0 12 T1952-p sLsLGtItDEEMKTG
0 42 S1969-p QETLRRAsMQPIQIA
0 1 K1988-a ITTRQQRkRVsLEPH
0 40 S1991-p RQQRkRVsLEPHQGP
0 10 L1992 QQRkRVsLEPHQGPG
0 5 P1994 RkRVsLEPHQGPGtP
0 57 T2000-p EPHQGPGtPEskKAT
0 3 S2003-p QGPGtPEskKATSCF
0 4 K2004-a GPGtPEskKATSCFP
0 33 T2015-p SCFPRPMtPRDRHEG
0 23 S2026-p RHEGRKQsttEAQKK
0 2 T2027-p HEGRKQsttEAQKKA
0 1 T2028-p EGRKQsttEAQKKAA
0 4 S2047-p KQADRRQsMAFsILN
0 1 S2051-p RRQsMAFsILNtPkK
1 23 T2055-p MAFsILNtPkKLGNs
0 2 K2057-a FsILNtPkKLGNsLL
0 1 K2058 sILNtPkKLGNsLLR
0 2 S2062-p tPkKLGNsLLRRGAS
0 2 K2070-a LLRRGASkkALsKAs
0 2 K2071-a LRRGASkkALsKAsP
0 1 S2074-p GASkkALsKAsPNtR
0 25 S2077-p kkALsKAsPNtRSGT
0 1 T2080-p LsKAsPNtRSGTRRS
0 1 T2093 RSPRIATTTASAATA
0 17 T2106-p TAAAIGAtPRAKGKA
3429 : Phospho-NuMA (Ser395) Antibody
  mouse

 
T2 ______MTLHATRAA
T6 __MTLHATRAATLLS
T46 IINTIHDTKEGQQIL
Q54 KEGQQILQQPLPERL
S76 QKNRKHPSSTQCLVS
S77 KNRKHPSSTQCLVSV
S91 VQKVIEGSEMELAKM
T107 MLFLYQSTMSSRNLR
N112 QSTMSSRNLRDWEQF
S167-p STLSEELsPPSHQTK
S170 SEELsPPSHQTKRKI
S198 ENNFLSGSPsSPMGD
S200-p NFLSGSPsSPMGDIL
S201 FLSGSPsSPMGDILQ
T209 PMGDILQTPQFQMRR
K245-u SLKLLTEkDAQIAMM
K265 HLALLNEKQAAsSQE
S269-p LNEKQAAsSQEPSEL
R309 TEKSQMDRKISQLSE
K324 ENGDLSFKVREFANH
K356 ASQEWAEKQARLENE
N362 EKQARLENELSTALQ
K377 DKKCLEEKNEILQGK
K384 KNEILQGKLSQLEDQ
S386 EILQGKLSQLEDQAT
T393 SQLEDQATRLQEsPA
S398-p QATRLQEsPAPEKGE
K524-a SLKEQAKkEQAQMLQ
K555 EQLSSSLKLKEQQLE
S589-p VAEAREAsLRERDTA
Q598 RERDTARQQLETVEK
K610 VEKEKDAKLESLQQQ
T630 DARDNAQTSVTQAQQ
S631 ARDNAQTSVTQAQQE
R814 RQKRFEARQQEEARY
- gap
K878 AIQQVQEKEVRAQKL
K878 AIQQVQEKEVRAQKL
K895 DLSALQEKMAATNKE
K926 TASLELLKEPPRAAN
T950 QGRPFSSTHAAVKAM
S978 LRAALIKSQGQQQEE
S1099 GASGEDASGPGTQSE
S1105 ASGPGTQSETAGKTD
E1117 KTDAPGPELQALRAE
G1141 QQQQQVEGLTHSLKS
S1183 HNQAASASAQRELQA
S1220-p QQEAERKssLIssLE
S1221-p QEAERKssLIssLEE
S1224-p ERKssLIssLEEEVS
S1225-p RKssLIssLEEEVSI
S1258 VVAESEKSQKLEERL
S1274 LLQVETASNSARAAE
S1276 QVETASNSARAAERS
K1326 EELGQELKAWQEKFF
K1331 ELKAWQEKFFQKEQA
K1426 AQQLRAEKASYAEQL
K1471-u ELDQAREkYVQELAA
T1485 AVRTDAETHLAEMRQ
K1507 ELEVMTAKYEGAKVK
K1507 ELEVMTAKYEGAKVK
K1555 KVQQQKLKAFQAQRG
S1564 FQAQRGESQQEVQRL
S1583 NELQAQLSQKEQAAE
K1606 AKTHYDAKKQQNQKL
K1681 QQLRDLGKFQVATDA
K1690 QVATDALKSREPQVK
S1703-p VKPQLDLsIDsLDLs
S1706-p QLDLsIDsLDLsLEE
S1710-p sIDsLDLsLEEGTPC
T1715 DLsLEEGTPCSVASK
T1726-p VASKLPRtQPDGTSV
T1731 PRtQPDGTSVPGEPA
S1732 RtQPDGTSVPGEPAs
S1739-p SVPGEPAsPIsQRLP
S1742-p GEPAsPIsQRLPPKV
K1748 IsQRLPPKVEsLEsL
K1748 IsQRLPPKVEsLEsL
K1748 IsQRLPPKVEsLEsL
S1751-p RLPPKVEsLEsLYFt
S1754-p PKVEsLEsLYFtPTP
Y1756 VEsLEsLYFtPTPAR
T1758-p sLEsLYFtPTPARGQ
T1770-p RGQAPLEtsLDsLGD
S1771-p GQAPLEtsLDsLGDA
S1774-p PLEtsLDsLGDAFPD
S1782-p LGDAFPDsGRKTRSA
T1786 FPDsGRKTRSARRRt
S1788 DsGRKTRSARRRttQ
T1793-p TRSARRRttQIINIT
T1794-p RSARRRttQIINITM
T1800 ttQIINITMTKKLEL
T1802 QIINITMTKKLELEE
D1811 KLELEEPDSANSSFY
S1812 LELEEPDSANSSFYS
S1815 EEPDSANSSFYSTQS
S1816 EPDSANSSFYSTQSA
Y1818 DSANSSFYSTQSAPA
S1819 SANSSFYSTQSAPAs
T1820 ANSSFYSTQSAPAsQ
S1822 SSFYSTQSAPAsQAN
S1826-p STQSAPAsQANLRAT
S1826 STQSAPASQANLRAT
N1829 SAPAsQANLRATSsT
S1834 QANLRATSsTQsLAR
S1835-p ANLRATSsTQsLARL
T1836 NLRATSsTQsLARLG
S1838-p RATSsTQsLARLGsP
S1844-p QsLARLGsPDDGNsA
D1847 ARLGsPDDGNsALLS
S1850-p GsPDDGNsALLSLPG
S1854 DGNsALLSLPGYRPT
T1861 SLPGYRPTTRSSARR
S1869 TRSSARRSQArMSSG
R1872-m1 SARRSQArMSSGAPQ
S1874 RRSQArMSSGAPQGR
S1883 GAPQGRNSFYMGTCQ
Y1885 PQGRNSFYMGTCQDE
S1924 KTCYPLESRPTLSLA
T1927 YPLESRPTLSLATIT
S1929 LESRPTLSLATITDE
T1932 RPTLSLATITDEEMK
T1934 TLSLATITDEEMKTG
S1951-p RETLRRAsMQPAQIA
K1970 ITTRQQRKRVssEtH
S1973-p RQQRKRVssEtHQGP
S1974-p QQRKRVssEtHQGPG
T1976-p RKRVssEtHQGPGtP
T1982-p EtHQGPGtPESKKAT
S1985 QGPGtPESKKATSCF
K1986 GPGtPESKKATSCFP
T1997-p SCFPRPMtPRDRHEG
S2008 RHEGRKQSSTADTQK
S2009 HEGRKQSSTADTQKK
T2010 EGRKQSSTADTQKKA
S2029-p KQADRRQsMAFSILN
S2033 RRQsMAFSILNtPKk
T2037-p MAFSILNtPKkLGNs
K2039 FSILNtPKkLGNsLL
K2040-u SILNtPKkLGNsLLR
S2044-p tPKkLGNsLLRRGAS
K2052 LLRRGASKKTPAKVs
K2053 LRRGASKKTPAKVsP
A2056 GASKKTPAKVsPNPR
S2059-p KKTPAKVsPNPRSGT
P2062 PAKVsPNPRSGTRRS
T2075-p RSPRIATtTTGTATV
T2085-p GTATVATtPRAKGKV
3429 : Phospho-NuMA (Ser395) Antibody
  rat

 
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
K1 _______KYVQELAA
T15 AVRTDAETHLAEMRQ
K37 ELEVMTAKYESAKVK
K37 ELEVMTAKYESAKVK
K99 RVQQQKLKAFQAQGG
S108 FQAQGGESQQEVQCL
S127 SELQAQLSQKEQAAE
K150 AKTHYDAKKQQNQEL
K225 QQLRDLGKFQVATDA
K234 QVATDALKSREPQVK
S247 VKPQLDLSIDSLDLS
S250 QLDLSIDSLDLSLEE
S254 SIDSLDLSLEEGTPC
T259 DLSLEEGTPCSVASK
T270 VASKLPRTQPDGTSV
T275 PRTQPDGTSVPGEPA
S276 RTQPDGTSVPGEPAs
S283-p SVPGEPAsPISQRLP
S286 GEPAsPISQRLPPKV
K292 ISQRLPPKVESLESL
K292 ISQRLPPKVESLESL
K292 ISQRLPPKVESLESL
S295 RLPPKVESLESLYFT
S298 PKVESLESLYFTPIP
Y300 VESLESLYFTPIPAR
T302 SLESLYFTPIPARGQ
T314 RGQAPLETSLDSLGD
S315 GQAPLETSLDSLGDA
S318 PLETSLDSLGDAFPD
S326 LGDAFPDSGRKTRSA
T330 FPDSGRKTRSARRRt
S332 DSGRKTRSARRRtTQ
T337-p TRSARRRtTQIINIT
T338 RSARRRtTQIINITM
T344 tTQIINITMTKKLEL
T346 QIINITMTKKLELEE
D355 KLELEEPDSANSSFY
S356 LELEEPDSANSSFYS
S359 EEPDSANSSFYSTQS
S360 EPDSANSSFYSTQSA
Y362 DSANSSFYSTQSAPA
S363 SANSSFYSTQSAPAS
T364 ANSSFYSTQSAPASQ
S366 SSFYSTQSAPASQAN
S370 STQSAPASQANLRAT
S370 STQSAPASQANLRAT
N373 SAPASQANLRATSST
S378 QANLRATSSTQSLAR
S379 ANLRATSSTQSLARL
T380 NLRATSSTQSLARLG
S382 RATSSTQSLARLGSP
S388 QSLARLGSPDDSNSA
D391 ARLGSPDDSNSALLS
S394 GSPDDSNSALLSLPG
S398 DSNSALLSLPGYRPT
T405 SLPGYRPTTRSSARR
S413 TRSSARRSQTRTSSG
R416 SARRSQTRTSSGAPH
S418 RRSQTRTSSGAPHGR
S427 GAPHGRNSFYMGTCQ
Y429 PHGRNSFYMGTCQDE
S468 KTCYPLESRPALSLA
A471 YPLESRPALSLATIT
S473 LESRPALSLATITDE
T476 RPALSLATITDEEMK
T478 ALSLATITDEEMKTG
S495-p RETLRRAsMQPAQIA
K514 ITTRQQRKRVSSETH
S517 RQQRKRVSSETHQGP
S518 QQRKRVSSETHQGPG
T520 RKRVSSETHQGPGTP
T526 ETHQGPGTPESKKAT
S529 QGPGTPESKKATSCF
K530 GPGTPESKKATSCFP
T541 SCFPRPMTPRDRHEG
N552 RHEGRKQNSTADAQK
S553 HEGRKQNSTADAQKK
T554 EGRKQNSTADAQKKA
S573 KQSDRRQSMAFSILN
S577 RRQSMAFSILNtPKK
T581-p MAFSILNtPKKLGNS
K583 FSILNtPKKLGNSLL
K584 SILNtPKKLGNSLLR
S588 tPKKLGNSLLRRGTS
K596 LLRRGTSKKTPAKVS
K597 LRRGTSKKTPAKVSP
A600 GTSKKTPAKVSPNPR
S603 KKTPAKVSPNPRSGT
P606 PAKVSPNPRSGTRRS
T619 RSPRIATTTAGAATA
T629 GAATAATTPRAKGKA
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.