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Protein Page:
NIPA (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
NIPA a nuclear protein that interacts with anaplastic lymphoma kinase (ALK). An essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1. Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting cyclin B1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of cyclin B1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events. Interacts with the NPM-ALK fusion protein in a tyrosine phosphorylation-dependent manner. Three splice-variant isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Cell cycle regulation; Ubiquitin conjugating system
Cellular Component: nuclear membrane; nucleus
Molecular Function: protein binding; zinc ion binding; protein kinase binding
Biological Process: mitosis; protein ubiquitination
Reference #:  Q86WB0 (UniProtKB)
Alt. Names/Synonyms: 1110054L24Rik; hematopoietic stem/progenitor cell protein 216; hNIPA; HSPC216; NIPA; nuclear interacting partner of anaplastic lymphoma kinase (ALK); nuclear interaction partner of ALK; Nuclear-interacting partner of ALK; Nuclear-interacting partner of anaplastic lymphoma kinase; ZC3HC1; Zinc finger C3HC-type protein 1; zinc finger, C3HC type 1; zinc finger, C3HC-type containing 1
Gene Symbols: ZC3HC1
Molecular weight: 55,262 Da
Basal Isoelectric point: 5.44  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NIPA

Protein Structure Not Found.


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Sites Implicated In
apoptosis, altered: S354‑p
cell cycle regulation: S354‑p, S359‑p, S395‑p
molecular association, regulation: S354‑p, S359‑p, S395‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 P4 ____MAAPCEGQAFA
0 25 S24-p NWGAVVRsPEGtPQK
0 22 T28-p VVRsPEGtPQKIRQL
0 1 T52-p GGVDAKDtsATsQsV
0 1 S53-p GVDAKDtsATsQsVN
0 2 S56-p AKDtsATsQsVNGsP
0 7 S58-p DtsATsQsVNGsPQA
0 28 S62-p TsQsVNGsPQAEQPS
0 2 P68 GsPQAEQPSLESTSk
0 1 K75-u PSLESTSkEAFFSRV
1 2 T84-p AFFSRVEtFSsLKWA
0 2 S87-p SRVEtFSsLKWAGKP
0 5 S161-p KFCFWPDsPSPDRFG
0 23 S321-p RLPLVPEsPRRMMTR
0 6 S329-p PRRMMTRsQDAtFsP
0 3 T333-p MTRsQDAtFsPGsEQ
0 24 S335-p RsQDAtFsPGsEQAE
0 14 S338-p DAtFsPGsEQAEKsP
0 5 A341 FsPGsEQAEKsPGPI
0 39 S344-p GsEQAEKsPGPIVsR
0 3 S350-p KsPGPIVsRtRsWDs
0 5 T352-p PGPIVsRtRsWDsss
4 49 S354-p PIVsRtRsWDsssPV
0 6 S357-p sRtRsWDsssPVDRP
0 7 S358-p RtRsWDsssPVDRPE
2 21 S359-p tRsWDsssPVDRPEP
1 32 S370-p RPEPEAAsPttRTRP
0 1 T372-p EPEAAsPttRTRPVT
0 2 T373-p PEAAsPttRTRPVTR
0 3 S381-p RTRPVTRsMGtGDtP
0 3 T384-p PVTRsMGtGDtPGLE
0 11 T387-p RsMGtGDtPGLEVPs
1 15 S394-p tPGLEVPssPLRKAK
3 48 S395-p PGLEVPssPLRKAKR
0 480 S407-p AKRARLCsssssDts
0 51 S408-p KRARLCsssssDtss
0 58 S409-p RARLCsssssDtssR
0 135 S410-p ARLCsssssDtssRS
0 133 S411-p RLCsssssDtssRSF
0 16 T413-p CsssssDtssRSFFD
0 5 S414-p sssssDtssRSFFDP
0 4 S415-p ssssDtssRSFFDPT
0 1 S423 RSFFDPTSQHRDWCP
0 2 S471-p ILLAHKQssQPAEtD
0 2 S472-p LLAHKQssQPAEtDs
0 2 T477-p QssQPAEtDsMsLsE
0 2 S479-p sQPAEtDsMsLsEks
0 1 S481-p PAEtDsMsLsEksRK
0 2 S483-p EtDsMsLsEksRKVF
0 1 K485-u DsMsLsEksRKVFRI
0 1 S486-p sMsLsEksRKVFRIF
  mouse

► Hide Isoforms
 
T4-p ____MAAtSEGPLFA
S24-p TWGSVVRsPEGtPQK
T28-p VVRsPEGtPQKVREL
T52 GGTEPKDTAATFQsV
A53 GTEPKDTAATFQsVD
F56 PKDTAATFQsVDGsP
S58-p DTAATFQsVDGsPQA
S62-p TFQsVDGsPQAEQsP
S68-p GsPQAEQsPLESTSK
K75 sPLESTSKEAFFHRV
T84 AFFHRVETFSSLKWA
S87 HRVETFSSLKWAGKP
S161 KFCFWPDSPSPDRFG
S320 HFPLVPESPRRMMTR
S328-p PRRMMTRsQDAtVsP
T332-p MTRsQDAtVsPGsEQ
S334-p RsQDAtVsPGsEQsE
S337-p DAtVsPGsEQsEKsP
S340-p VsPGsEQsEKsPGPI
S343-p GsEQsEKsPGPIVsR
S349-p KsPGPIVsRtRsWEs
T351-p PGPIVsRtRsWEsss
S353-p PIVsRtRsWEsssPV
S356-p sRtRsWEsssPVDRP
S357-p RtRsWEsssPVDRPE
S358-p tRsWEsssPVDRPEL
S369-p RPELEAAsPTTRSRP
T371 ELEAAsPTTRSRPVT
T372 LEAAsPTTRSRPVTR
S380 RSRPVTRSMGTGDSA
T383 PVTRSMGTGDSAGVE
S386 RSMGTGDSAGVEVPs
S393-p SAGVEVPssPLRRTK
S394-p AGVEVPssPLRRTKR
S406-p TKRARLCsssssDts
S407-p KRARLCsssssDtsP
S408-p RARLCsssssDtsPR
S409-p ARLCsssssDtsPRS
S410-p RLCsssssDtsPRSF
T412-p CsssssDtsPRSFFD
S413-p sssssDtsPRSFFDP
P414 ssssDtsPRSFFDPT
S422 RSFFDPTSQHRDWCP
S470 ILLAHKRSNQPAETD
N471 LLAHKRSNQPAETDS
T476 RSNQPAETDSMSLSE
S478 NQPAETDSMSLSEKS
S480 PAETDSMSLSEKSRK
S482 ETDSMSLSEKSRKVF
K484 DSMSLSEKSRKVFRI
S485 SMSLSEKSRKVFRIF
  NIPA iso2  
T4 ____MAATSEGPLFA
S24 TWGSVVRSPEGTPQK
T28 VVRSPEGTPQKVREL
T52 GGTEPKDTAATFQSV
A53 GTEPKDTAATFQSVD
F56 PKDTAATFQSVDGSP
S58 DTAATFQSVDGSPQA
S62 TFQSVDGSPQAEQSP
S68 GSPQAEQSPLESTSK
K75 SPLESTSKEAFFHRV
T84 AFFHRVETFSSLKWA
S87 HRVETFSSLKWAGKP
S161 KFCFWPDSPSPDRFG
S320 HFPLVPESPRRMMTR
S328 PRRMMTRSQDATVSP
T332 MTRSQDATVSPGSEQ
S334 RSQDATVSPGSEQSE
S337 DATVSPGSEQSEKSP
S340 VSPGSEQSEKSPGPI
S343 GSEQSEKSPGPIVSR
S349 KSPGPIVSRTRSWES
T351 PGPIVSRTRSWESSS
S353 PIVSRTRSWESSSPV
S356 SRTRSWESSSPVDRP
S357 RTRSWESSSPVDRPE
S358 TRSWESSSPVDRPEL
S369 RPELEAASPTTRSRP
T371 ELEAASPTTRSRPVT
T372 LEAASPTTRSRPVTR
S380 RSRPVTRSMGTGDSA
T383 PVTRSMGTGDSAGVE
S386 RSMGTGDSAGVEVPS
S393 SAGVEVPSSPLRRTK
S394 AGVEVPSSPLRRTKR
S406 TKRARLCSSSSSDTS
S407 KRARLCSSSSSDTSP
S408 RARLCSSSSSDTSPR
S409 ARLCSSSSSDTSPRS
S410 RLCSSSSSDTSPRSF
T412 CSSSSSDTSPRSFFD
S413 SSSSSDTSPRSFFDP
P414 SSSSDTSPRSFFDPT
S422 RSFFDPTSQHRDWCP
S470 ILLAHKRSNQPAETD
N471 LLAHKRSNQPAETDS
T476 RSNQPAETDSMLK__
S478 NQPAETDSMLK____
- gap
- gap
- gap
- gap
  NIPA iso3  
T4 ____MAATSEGPLFA
S24 TWGSVVRSPEGTPQK
T28 VVRSPEGTPQKVREL
T52 GGTEPKDTAATFQSV
A53 GTEPKDTAATFQSVD
F56 PKDTAATFQSVDGSP
S58 DTAATFQSVDGSPQA
S62 TFQSVDGSPQAEQSP
S68 GSPQAEQSPLESTSK
K75 SPLESTSKEAFFHRV
T84 AFFHRVETFSSLKWA
S87 HRVETFSSLKWAGKP
S161 KFCFWPDSPSPDRFG
S320 HFPLVPESPRRMMTR
S328-p PRRMMTRsQDATVSP
T332 MTRsQDATVSPGSEQ
S334 RsQDATVSPGSEQDT
S337 DATVSPGSEQDTSPR
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
T341 SPGSEQDTSPRSFFD
S342 PGSEQDTSPRSFFDP
P343 GSEQDTSPRSFFDPT
S351-p RSFFDPTsQHRDWCP
S399 ILLAHKRSNQPAETD
N400 LLAHKRSNQPAETDS
T405 RSNQPAETDSMSLSE
S407 NQPAETDSMSLSEKS
S409 PAETDSMSLSEKSRK
S411 ETDSMSLSEKSRKVF
K413 DSMSLSEKSRKVFRI
S414 SMSLSEKSRKVFRIF
  rat

 
S4 ____MAASCEGQLFA
S24 TWGAVVRSPEGTPQK
T28 VVRSPEGTPQKVRQL
T52 GGTEPKDTSATFQSV
S53 GTEPKDTSATFQSVD
F56 PKDTSATFQSVDGsP
S58 DTSATFQSVDGsPQA
S62-p TFQSVDGsPQAEQPP
P68 GsPQAEQPPLESTSK
K75 PPLESTSKEAFFHRV
T84 AFFHRVETFSSLKWA
S87 HRVETFSSLKWAGKP
S161 KFCFWPDSPSPDRFG
S320 HFPLVPESPRRMMTR
S328 PRRMMTRSQDAAVsP
A332 MTRSQDAAVsPSSEQ
S334-p RSQDAAVsPSSEQsE
S337 DAAVsPSSEQsEKSP
S340-p VsPSSEQsEKSPGPI
S343 SSEQsEKSPGPIVSR
S349 KSPGPIVSRtRsWES
T351-p PGPIVSRtRsWESSS
S353-p PIVSRtRsWESSSPV
S356 SRtRsWESSSPVDRP
S357 RtRsWESSSPVDRPE
S358 tRsWESSSPVDRPEL
S369 RPELEAASPTtRSRP
T371 ELEAASPTtRSRPVT
T372-p LEAASPTtRSRPVTR
S380 RSRPVTRSMGTGDSA
T383 PVTRSMGTGDSAGLE
S386 RSMGTGDSAGLEVPS
S393 SAGLEVPSsPHRRAK
S394-p AGLEVPSsPHRRAKR
S406-p AKRPRLCsSSsSDTS
S407 KRPRLCsSSsSDTSP
S408 RPRLCsSSsSDTSPR
S409-p PRLCsSSsSDTSPRS
S410 RLCsSSsSDTSPRSF
T412 CsSSsSDTSPRSFFD
S413 sSSsSDTSPRSFFDP
P414 SSsSDTSPRSFFDPT
S422 RSFFDPTSQHRDWCP
S470 ILLAHKHSNQPAETD
N471 LLAHKHSNQPAETDS
T476 HSNQPAETDSMSLSE
S478 NQPAETDSMSLSEKS
S480 PAETDSMSLSEKSRK
S482 ETDSMSLSEKSRKVF
K484 DSMSLSEKSRKVFRI
S485 SMSLSEKSRKVFRIF
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