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Protein Page:
Smad4 (mouse)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
Smad4 transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription. Note: This description may include information from UniProtKB.
Protein type: Transcription, coactivator/corepressor; DNA binding protein
Cellular Component: centrosome; transcription factor complex; protein complex; cytoplasm; intracellular; nucleus
Molecular Function: collagen binding; identical protein binding; protein binding; protein homodimerization activity; transforming growth factor beta receptor, common-partner cytoplasmic mediator activity; DNA binding; sequence-specific DNA binding; metal ion binding; chromatin binding; SMAD binding; filamin binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; developmental growth; axon guidance; positive regulation of transcription, DNA-dependent; sebaceous gland development; gastrulation; palate development; BMP signaling pathway; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; mesoderm development; neural crest cell differentiation; kidney development; positive regulation of BMP signaling pathway; regulation of transforming growth factor-beta2 production; tissue morphogenesis; transcription, DNA-dependent; regulation of binding; in utero embryonic development; positive regulation of transforming growth factor beta receptor signaling pathway; neuron fate commitment; gastrulation with mouth forming second; somite rostral/caudal axis specification; formation of anatomical boundary; SMAD protein complex assembly; endothelial cell activation; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; cell proliferation; ureteric bud branching; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; regulation of hair follicle development; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; negative regulation of protein catabolic process; endoderm development
Reference #:  P97471 (UniProtKB)
Alt. Names/Synonyms: AW743858; D18Wsu70e; Deletion target in pancreatic carcinoma 4 homolog; Dpc4; MAD homolog 4; MAD homolog 4 (Drosophila); Madh4; Mothers against decapentaplegic homolog 4; Mothers against DPP homolog 4; OTTMUSP00000023201; OTTMUSP00000023202; SMAD 4; SMAD family member 4; Smad4
Gene Symbols: Smad4
Molecular weight: 60,342 Da
Basal Isoelectric point: 6.5  Predict pI for various phosphorylation states
CST Pathways:  ESC Pluripotency and Differentiation  |  G1/S Checkpoint  |  SAPK/JNK Signaling Cascades  |  TGF-├č Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Smad4

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  Source  |  UCSD-Nature  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
transcription, induced: T276‑p
intracellular localization: T276‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
1 0 T9 DNMSITNTPTSNDAC
0 1 K37 GESETFAKRAIESLV
0 1 K45 RAIESLVKKLkEKKD
0 1 K45-u RAIESLVkKLkEKKD
0 6 K48-a ESLVkKLkEKKDELD
0 2 K70 TNGAHPSKCVTIQRT
1 0 T77 KCVTIQRTLDGRLQV
0 1 K106 WRWPDLHKNELKHVk
0 3 K113-u KNELKHVkYCQYAFD
4 0 K113 KNELKHVKYCQYAFD
1 0 S138 YHYERVVSPGIDLSG
6 0 K158 NAPSMLVKDEYVHDF
1 0 T276-p GSRTAPYtPNLPHHQ
1 0 S342 GETFKVPSSCPVVTV
0 1 K427 APGDAVHKIYPSAYI
0 1 K506 ILRMSFVKGWGPDYP
1 0 K506 ILRMSFVKGWGPDYP
1 1 K518 DYPRQSIKETPCWIE
  human

 
T9-p DNMSITNtPTSNDAC
K37-a GESETFAkRAIESLV
K45-a RAIESLVkKLkEKKD
K45 RAIESLVKKLkEKKD
K48-a ESLVkKLkEKKDELD
K70-u TNGAHPSkCVTIQRt
T77-p kCVTIQRtLDGRLQV
K106-a WRWPDLHkNELKHVk
K113-u kNELKHVkYCQYAFD
K113-s kNELKHVkYCQYAFD
S138-p YHYERVVsPGIDLSG
K159-s APSSMMVkDEYVHDF
T277 GSRTAPYTPNLPHHQ
S343-p GETFKVPsSCPIVTV
K428-a APGDAVHkIYPSAYI
K507-a ILRMSFVkGWGPDYP
K507-u ILRMSFVkGWGPDYP
K519-u DYPRQSIkETPCWIE
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