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Protein Page:
GFAP (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
GFAP a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined. Note: This description may include information from UniProtKB.
Protein type: Cytoskeletal protein
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: membrane; cytoplasm; intermediate filament; cytosol
Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding
Biological Process: extracellular matrix organization and biogenesis; Bergmann glial cell differentiation; regulation of neurotransmitter uptake; response to wounding; intermediate filament organization; neurite regeneration; astrocyte development
Reference #:  P14136 (UniProtKB)
Alt. Names/Synonyms: FLJ45472; GFAP; Glial fibrillary acidic protein
Gene Symbols: GFAP
Molecular weight: 49,880 Da
Basal Isoelectric point: 5.42  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

GFAP

Protein Structure Not Found.


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Sites Implicated In
molecular association, regulation: S8‑p, S13‑p
protein stabilization: T7‑p, S8‑p, S13‑p, S17‑p, S38‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
6 0 T7-p _MERRRItsAArrsy
8 0 S8-p MERRRItsAArrsyV
0 1 R11-m1 RRItsAArrsyVSsG
0 1 R11-m2 RRItsAArrsyVSsG
0 2 R12-m1 RItsAArrsyVSsGE
10 3 S13-p ItsAArrsyVSsGEM
0 3 Y14-p tsAArrsyVSsGEMM
1 2 S17-p ArrsyVSsGEMMVGG
0 1 - gap
0 4 - gap
0 1 R29 VGGLAPGRRLGPGTR
0 1 R36 RRLGPGTRLsLARMP
0 1 R36 RRLGPGTRLsLARMP
6 0 S38-p LGPGTRLsLARMPPP
0 3 P43 RLsLARMPPPLPTRV
0 1 K86 RFASYIEKVRFLEQQ
0 9 K86 RFASYIEKVRFLEQQ
1 1 K95-ac RFLEQQNkALAAELN
0 17 K95 RFLEQQNKALAAELN
0 1 K107 ELNQLRAKEPTKLAD
0 1 T110 QLRAKEPTKLADVyQ
0 6 K111 LRAKEPTKLADVyQA
0 3 Y116-p PTKLADVyQAELREL
0 1 T131-p RLRLDQLtANSARLE
0 2 A145 EVERDNLAQDLAtVR
0 3 T150-p NLAQDLAtVRQkLQD
1 1 K154-ac DLAtVRQkLQDETNL
0 5 Y172-p AENNLAAyRQEADEA
1 1 K189-ac ARLDLERkIESLEEE
0 1 K189 ARLDLERKIESLEEE
0 11 K202 EEIRFLRKIHEEEVR
1 1 K228-ac HVELDVAkPDLTAAL
0 1 T240-p AALKEIRtQyEAMAS
1 3 Y242-p LKEIRtQyEAMASSN
1 3 K260-ac AEEWYRSkFADLTDA
0 1 K260 AEEWYRSKFADLTDA
0 2 T293 RRQLQSLTCDLESLR
0 4 S305-p SLRGTNEsLERQMRE
0 1 S323 RHVREAASyQEALAR
0 5 Y324-p HVREAASyQEALARL
1 1 K339-ac EEEGQSLkDEMARHL
0 2 K339 EEEGQSLKDEMARHL
0 6 K368 IEIATYRKLLEGEEN
0 1 S385 TIPVQTFSNLQIRET
0 1 K397 RETSLDTKSVSEGHL
0 1 - gap
0 2 K405 SVSEGHLKRNIVVKT
0 4 K411 LKRNIVVKTVEMRDG
  GFAP iso2  
T7 _MERRRITSAARRSY
S8 MERRRITSAARRSYV
R11 RRITSAARRSYVSSG
R11 RRITSAARRSYVSSG
R12 RITSAARRSYVSSGE
S13 ITSAARRSYVSSGEM
Y14 TSAARRSYVSSGEMM
S17 ARRSYVSSGEMMVGG
- gap
- gap
R29 VGGLAPGRRLGPGTR
R36 RRLGPGTRLSLARMP
R36 RRLGPGTRLSLARMP
S38 LGPGTRLSLARMPPP
P43 RLSLARMPPPLPTRV
K86 RFASYIEKVRFLEQQ
K86 RFASYIEKVRFLEQQ
K95 RFLEQQNKALAAELN
K95 RFLEQQNKALAAELN
K107 ELNQLRAKEPTKLAD
T110 QLRAKEPTKLADVYQ
K111 LRAKEPTKLADVYQA
Y116 PTKLADVYQAELREL
T131 RLRLDQLTANSARLE
A145 EVERDNLAQDLATVR
T150 NLAQDLATVRQKLQD
K154 DLATVRQKLQDETNL
Y172 AENNLAAYRQEADEA
K189 ARLDLERKIESLEEE
K189 ARLDLERKIESLEEE
K202 EEIRFLRKIHEEEVR
K228 HVELDVAKPDLTAAL
T240 AALKEIRTQYEAMAS
Y242 LKEIRTQYEAMASSN
K260 AEEWYRSKFADLTDA
K260 AEEWYRSKFADLTDA
T293 RRQLQSLTCDLESLR
S305 SLRGTNESLERQMRE
S323 RHVREAASYQEALAR
Y324 HVREAASYQEALARL
K339 EEEGQSLKDEMARHL
K339 EEEGQSLKDEMARHL
K368 IEIATYRKLLEGEEN
S385 TIPVQTFSNLQIRGQ
- gap
S397-p RGQYSRAsWEGHWSP
- gap
- gap
  mouse

 
T7 _MERRRITsARrsYA
S8-p MERRRITsARrsYAs
R10 RRRITsARrsYAsEt
R10 RRRITsARrsYAsEt
R11-m1 RRITsARrsYAsEtV
S12-p RITsARrsYAsEtVV
Y13 ITsARrsYAsEtVVr
S15-p sARrsYAsEtVVrGL
T17-p RrsYAsEtVVrGLGP
R20-m1 YAsEtVVrGLGPSrQ
R26-m1 VrGLGPSrQLGTMPr
R33-m1 rQLGTMPrFSLSRMt
R33-m2 rQLGTMPrFSLSRMt
S35 LGTMPrFSLSRMtPP
T40-p rFSLSRMtPPLPARV
K83 RFASYIEKVRFLEQQ
K83-ub RFASYIEkVRFLEQQ
K92 RFLEQQNKALAAELN
K92-ub RFLEQQNkALAAELN
K104-ub ELNQLRAkEPtkLAD
T107-p QLRAkEPtkLADVyQ
K108-ub LRAkEPtkLADVyQA
Y113-p PtkLADVyQAELREL
T128 RLRLDQLTANSARLE
A142 EVERDNFAQDLGtLR
T147-p NFAQDLGtLRQKLQD
K151 DLGtLRQKLQDETNL
Y169 AENNLAAYRQEADEA
K186 ARVDLERKVESLEEE
K186-ub ARVDLERkVESLEEE
K199-ub EEIQFLRkIYEEEVR
K225 HVEMDVAKPDLTAAL
T237 AALREIRTQYEAVAT
Y239 LREIRTQYEAVATSN
K257 TEEWYRSKFADLTDA
K257-ub TEEWYRSkFADLTDA
T290 RRQLQALTCDLESLR
S302-p SLRGTNEsLERQMRE
S320-p RHARESAsyQEALAR
Y321-p HARESAsyQEALARL
K336 EEEGQSLKEEMARHL
K336-ub EEEGQSLkEEMARHL
K365-ub IEIATYRkLLEGEEN
S382-p TIPVQTFsNLQIRET
K394-ub RETSLDTkSVSEGHL
- gap
K402 SVSEGHLKRNIVVkT
K408-ub LKRNIVVkTVEMRDG
  rat

 
T7 _MERRRITSARRsYA
S8 MERRRITSARRsYAS
R10 RRRITSARRsYASSE
R10 RRRITSARRsYASSE
R11 RRITSARRsYASSET
S12-p RITSARRsYASSETM
Y13 ITSARRsYASSETMV
S16 ARRsYASSETMVRGH
T18 RsYASSETMVRGHGP
R21 ASSETMVRGHGPTRH
R27 VRGHGPTRHLGTIPR
R34 RHLGTIPRLsLSRMt
R34 RHLGTIPRLsLSRMt
S36-p LGTIPRLsLSRMtPP
T41-p RLsLSRMtPPLPARV
K84-ac RFASYIEkVRFLEQQ
K84-ub RFASYIEkVRFLEQQ
K93-ac RFLEQQNkALAAELN
K93-ub RFLEQQNkALAAELN
K105 ELNQLRAKEPTKLAD
T108 QLRAKEPTKLADVYQ
K109 LRAKEPTKLADVYQA
Y114 PTKLADVYQAELREL
T129 RLRLDQLTTNSARLE
T143-p EVERDNLtQDLGtLR
T148-p NLtQDLGtLRQkLQD
K152-ac DLGtLRQkLQDETNL
Y170 AENNLAVYRQEADEA
K187-ac ARVDLERkVESLEEE
K187 ARVDLERKVESLEEE
K200 EEIQFLRKIHEEEVR
K226 HVEMDVAKPDLTAAL
T238 AALREIRTQYEAVAT
Y240 LREIRTQYEAVATSN
K258-ac TEEWYRSkFADLTDV
K258 TEEWYRSKFADLTDV
T291-p RRQLQALtCDLESLR
S303-p SLRGTNEsLERQMRE
S321-p RHARESAsyQEALAR
Y322-p HARESAsyQEALARL
K337-ac EEEGQSLkEEMARHL
K337 EEEGQSLKEEMARHL
K366 IEIATYRKLLEGEEN
S383-p TIPVQTFsNLQIRET
K395 RETSLDTKSVSEGHL
- gap
K403-ub SVSEGHLkRNIVVKT
K409 LkRNIVVKTVEMRDG
  pig

 
T7-p _MERRRVtsAARRsY
S8-p MERRRVtsAARRsYV
R11 RRVtsAARRsYVSSL
R11 RRVtsAARRsYVSSL
R12 RVtsAARRsYVSSLE
S13-p VtsAARRsYVSSLET
Y14 tsAARRsYVSSLETV
S17 ARRsYVSSLETVGGG
- gap
- gap
R25 LETVGGGRRLGPGPR
R32 RRLGPGPRLsLARMP
R32 RRLGPGPRLsLARMP
S34-p LGPGPRLsLARMPPP
P39 RLsLARMPPPLPARV
K82 RFASYIEKCEVDSLR
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S94 SLRGTNESLERQMRE
S112 RHAREAASYQEALTR
Y113 HAREAASYQEALTRL
K128 EEEGQSLKDEMARHL
K128 EEEGQSLKDEMARHL
K157 IEIATYRKLLEGEEN
S174 TIPVQTFSNLQIRET
K186 RETSLDTKSVSEGHL
- gap
K194 SVSEGHLKRNVKTVE
K198 GHLKRNVKTVEMRDG
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