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Protein Page:
GFAP (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
GFAP a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined. Note: This description may include information from UniProtKB.
Protein type: Cytoskeletal protein
Cellular Component: membrane; cytoplasm; intermediate filament; cytosol
Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding
Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; astrocyte development
Reference #:  P14136 (UniProtKB)
Alt. Names/Synonyms: FLJ45472; GFAP; Glial fibrillary acidic protein
Gene Symbols: GFAP
Molecular weight: 49,880 Da
Basal Isoelectric point: 5.42  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

GFAP

Protein Structure Not Found.


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Sites Implicated In
molecular association, regulation: S8‑p, S13‑p
protein stabilization: T7‑p, S8‑p, S13‑p, S17‑p, S38‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
6 0 T7-p _MERRRItsAARrsy
8 0 S8-p MERRRItsAARrsyV
0 2 R12-m1 RItsAARrsyVSsGE
10 3 S13-p ItsAARrsyVSsGEM
0 3 Y14-p tsAARrsyVSsGEMM
1 1 S17-p ARrsyVSsGEMMVGG
0 4 - gap
0 1 R29 VGGLAPGRRLGPGTR
0 1 R36 RRLGPGTRLsLARMP
6 0 S38-p LGPGTRLsLARMPPP
0 3 P43 RLsLARMPPPLPTRV
0 9 K86 RFASYIEKVRFLEQQ
1 0 K95-a RFLEQQNkALAAELN
0 17 K95 RFLEQQNKALAAELN
0 1 T110 QLRAKEPTKLADVyQ
0 6 K111 LRAKEPTKLADVyQA
0 3 Y116-p PTKLADVyQAELREL
0 2 A145 EVERDNLAQDLAtVR
0 3 T150-p NLAQDLAtVRQkLQD
1 0 K154-a DLAtVRQkLQDETNL
0 6 Y172-p AENNLAAyRQEADEA
1 0 K189-a ARLDLERkIESLEEE
0 1 K189 ARLDLERKIESLEEE
0 11 K202 EEIRFLRKIHEEEVR
1 0 K228-a HVELDVAkPDLTAAL
1 3 Y242-p LKEIRTQyEAMASSN
1 2 K260-a AEEWYRSkFADLTDA
0 1 K260 AEEWYRSKFADLTDA
0 4 S305-p SLRGTNEsLERQMRE
0 1 S323 RHVREAASyQEALAR
0 5 Y324-p HVREAASyQEALARL
1 0 K339-a EEEGQSLkDEMARHL
0 2 K339 EEEGQSLKDEMARHL
0 6 K368 IEIATYRKLLEGEEN
0 1 S385 TIPVQTFSNLQIRET
0 1 K397 RETSLDTKSVSEGHL
0 2 K405 SVSEGHLKRNIVVKT
0 4 K411 LKRNIVVKTVEMRDG
  GFAP iso2  
T7 _MERRRITSAARRSY
S8 MERRRITSAARRSYV
R12 RITSAARRSYVSSGE
S13 ITSAARRSYVSSGEM
Y14 TSAARRSYVSSGEMM
S17 ARRSYVSSGEMMVGG
- gap
R29 VGGLAPGRRLGPGTR
R36 RRLGPGTRLSLARMP
S38 LGPGTRLSLARMPPP
P43 RLSLARMPPPLPTRV
K86 RFASYIEKVRFLEQQ
K95 RFLEQQNKALAAELN
K95 RFLEQQNKALAAELN
T110 QLRAKEPTKLADVYQ
K111 LRAKEPTKLADVYQA
Y116 PTKLADVYQAELREL
A145 EVERDNLAQDLATVR
T150 NLAQDLATVRQKLQD
K154 DLATVRQKLQDETNL
Y172 AENNLAAYRQEADEA
K189 ARLDLERKIESLEEE
K189 ARLDLERKIESLEEE
K202 EEIRFLRKIHEEEVR
K228 HVELDVAKPDLTAAL
Y242 LKEIRTQYEAMASSN
K260 AEEWYRSKFADLTDA
K260 AEEWYRSKFADLTDA
S305 SLRGTNESLERQMRE
S323 RHVREAASYQEALAR
Y324 HVREAASYQEALARL
K339 EEEGQSLKDEMARHL
K339 EEEGQSLKDEMARHL
K368 IEIATYRKLLEGEEN
S385 TIPVQTFSNLQIRGQ
- gap
- gap
- gap
  mouse

 
T7 _MERRRITsARrsYA
S8-p MERRRITsARrsYAs
R11-m1 RRITsARrsYAsETV
S12-p RITsARrsYAsETVV
Y13 ITsARrsYAsETVVr
S15-p sARrsYAsETVVrGL
R20-m1 YAsETVVrGLGPSrQ
R26-m1 VrGLGPSrQLGTMPr
R33-m2 rQLGTMPrFSLSRMt
S35 LGTMPrFSLSRMtPP
T40-p rFSLSRMtPPLPARV
K83-u RFASYIEkVRFLEQQ
K92 RFLEQQNKALAAELN
K92-u RFLEQQNkALAAELN
T107-p QLRAKEPtkLADVyQ
K108-u LRAKEPtkLADVyQA
Y113-p PtkLADVyQAELREL
A142 EVERDNFAQDLGtLR
T147-p NFAQDLGtLRQKLQD
K151 DLGtLRQKLQDETNL
Y169 AENNLAAYRQEADEA
K186 ARVDLERKVESLEEE
K186-u ARVDLERkVESLEEE
K199-u EEIQFLRkIYEEEVR
K225 HVEMDVAKPDLTAAL
Y239 LREIRTQYEAVATSN
K257 TEEWYRSKFADLTDA
K257-u TEEWYRSkFADLTDA
S302-p SLRGTNEsLERQMRE
S320-p RHARESAsyQEALAR
Y321-p HARESAsyQEALARL
K336 EEEGQSLKEEMARHL
K336-u EEEGQSLkEEMARHL
K365-u IEIATYRkLLEGEEN
S382-p TIPVQTFsNLQIRET
K394-u RETSLDTkSVSEGHL
K402 SVSEGHLKRNIVVkT
K408-u LKRNIVVkTVEMRDG
  rat

 
T7 _MERRRITSARRSYA
S8 MERRRITSARRSYAS
R11 RRITSARRSYASSET
S12 RITSARRSYASSETM
Y13 ITSARRSYASSETMV
S16 ARRSYASSETMVRGH
R21 ASSETMVRGHGPTRH
R27 VRGHGPTRHLGTIPR
R34 RHLGTIPRLSLSRMT
S36 LGTIPRLSLSRMTPP
T41 RLSLSRMTPPLPARV
K84-u RFASYIEkVRFLEQQ
K93 RFLEQQNKALAAELN
K93-u RFLEQQNkALAAELN
T108 QLRAKEPTKLADVYQ
K109 LRAKEPTKLADVYQA
Y114 PTKLADVYQAELREL
T143-p EVERDNLtQDLGTLR
T148 NLtQDLGTLRQKLQD
K152 DLGTLRQKLQDETNL
Y170-p AENNLAVyRQEADEA
K187 ARVDLERKVESLEEE
K187 ARVDLERKVESLEEE
K200 EEIQFLRKIHEEEVR
K226 HVEMDVAKPDLTAAL
Y240 LREIRTQYEAVATSN
K258-a TEEWYRSkFADLTDV
K258 TEEWYRSKFADLTDV
S303 SLRGTNESLERQMRE
S321 RHARESASyQEALAR
Y322-p HARESASyQEALARL
K337 EEEGQSLKEEMARHL
K337 EEEGQSLKEEMARHL
K366 IEIATYRKLLEGEEN
S383 TIPVQTFSNLQIRET
K395 RETSLDTKSVSEGHL
K403-u SVSEGHLkRNIVVKT
K409 LkRNIVVKTVEMRDG
  pig

 
T7-p _MERRRVtsAARRsY
S8-p MERRRVtsAARRsYV
R12 RVtsAARRsYVSSLE
S13-p VtsAARRsYVSSLET
Y14 tsAARRsYVSSLETV
S17 ARRsYVSSLETVGGG
- gap
R25 LETVGGGRRLGPGPR
R32 RRLGPGPRLsLARMP
S34-p LGPGPRLsLARMPPP
P39 RLsLARMPPPLPARV
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S94 SLRGTNESLERQMRE
S112 RHAREAASYQEALTR
Y113 HAREAASYQEALTRL
K128 EEEGQSLKDEMARHL
K128 EEEGQSLKDEMARHL
K157 IEIATYRKLLEGEEN
S174 TIPVQTFSNLQIRET
K186 RETSLDTKSVSEGHL
K194 SVSEGHLKRNVKTVE
K198 GHLKRNVKTVEMRDG
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