Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
JIP4 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
JIP4 The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg- interaction. Homooligomer. Interacts with MAX, MAPK8, MAPK9, MAPK10, MAPK14, MAP3K3, MYC, KNS2 and MAP2K4. Interaction with KNS2 is important in the formation of ternary complex with MAPK8. Interacts with NFKB1. Isoform 3 is increased in systemic sclerosis fibroblasts. Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis. Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients. Belongs to the JIP scaffold family. 6 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Cancer Testis Antigen (CTA); Adaptor/scaffold
Cellular Component: perinuclear region of cytoplasm; cytoplasm; integral to membrane; acrosome; cytosol
Molecular Function: JUN kinase binding; mitogen-activated protein kinase p38 binding; kinesin binding; phosphoinositide 3-kinase binding
Biological Process: muscle cell differentiation; positive regulation of MAPKKK cascade; striated muscle cell differentiation; positive regulation of muscle cell differentiation; spermatogenesis; positive regulation of neuron differentiation; retrograde transport, endosome to Golgi; activation of JNK activity; protein homooligomerization; positive regulation of cell migration
Reference #:  O60271 (UniProtKB)
Alt. Names/Synonyms: c-Jun NH2-terminal kinase-associated leucine zipper protein; C-Jun-amino-terminal kinase-interacting protein 4; Cancer/testis antigen 89; CT89; FLJ13450; FLJ14006; FLJ26141; FLJ34602; HLC-4 protein; HLC-6; HLC4; HSS; Human lung cancer oncogene 6 protein; JIP-4; JIP4; JLP; JNK interacting protein; JNK-associated leucine-zipper protein; JNK-interacting protein 4; JNK/SAPK-associated protein; KIAA0516; lung cancer oncogene 4; MAPK8IP4; Max-binding protein; MGC117291; MGC14967; MGC74461; Mitogen-activated protein kinase 8-interacting protein 4; PHET; PIG6; proliferation-inducing gene 6; Proliferation-inducing protein 6; Protein highly expressed in testis; SPAG9; sperm associated antigen 9; sperm specific protein; Sperm surface protein; Sperm-associated antigen 9; Sperm-specific protein; Sunday driver 1; SYD1
Gene Symbols: SPAG9
Molecular weight: 146,205 Da
Basal Isoelectric point: 5.05  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

JIP4
Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

► Hide Isoforms 		 
0 1 D5-ca ___MELEdGVVyQEE
0 2 Y9-p ELEdGVVyQEEPGGS
0 3 S109-p KFIEFEDsQEQEKKD
0 2 K178-u MEHLERTkLHQLsGs
0 8 S183-p RTkLHQLsGsDQLES
0 7 S185-p kLHQLsGsDQLESTA
0 1 S194-p QLESTAHsRIRKERP
0 1 K198 TAHsRIRKERPIsLG
0 23 S203-p IRKERPIsLGIFPLP
0 58 T217-p PAGDGLLtPDAQKGG
0 1 A220 DGLLtPDAQKGGEtP
0 20 T226-p DAQKGGEtPGsEQWk
0 5 S229-p KGGEtPGsEQWkFQE
0 2 K233-u tPGsEQWkFQELsQP
0 3 S238-p QWkFQELsQPRsHTs
0 3 S242-p QELsQPRsHTsLKVS
0 2 T244 LsQPRsHTsLKVSNs
0 6 S245-p sQPRsHTsLKVSNsP
0 3 S251-p TsLKVSNsPEPQKAV
0 12 S265-p VEQEDELsDVsQGGs
0 20 S268-p EDELsDVsQGGsKAt
0 6 S272-p sDVsQGGsKAtTPAS
0 1 T275-p sQGGsKAtTPASTAN
0 1 S279 sKAtTPASTANSDVA
0 1 S283 TPASTANSDVATIPt
0 1 T287 TANSDVATIPtDtPL
0 2 T290-p SDVATIPtDtPLKEE
0 10 T292-p VATIPtDtPLKEENE
0 1 S311-p VTDAPNKsEISKHIE
0 30 S329-p AQETRNVstGsAENE
0 5 T330-p QETRNVstGsAENEE
0 36 S332-p TRNVstGsAENEEKs
0 1 S339-p sAENEEKsEVQAIIE
0 1 S347-p EVQAIIEstPELDMD
0 3 T348-p VQAIIEstPELDMDK
0 1 S358 LDMDKDLSGYKGsSt
0 1 Y360 MDKDLSGYKGsStPt
0 2 S363-p DLSGYKGsStPtKGI
0 9 T365-p SGYKGsStPtKGIEN
0 1 T367-p YKGsStPtKGIENKA
0 5 T418-p ENLILENtQLLETKN
0 1 R505 AQRKRFTRVEMARVL
0 1 S538-p WTEMIRAsRENPAMQ
0 5 Y578-p EPPVNLKyNAPTSHV
1 3 T586-p NAPTSHVtPsVKKRs
0 1 S588-p PTSHVtPsVKKRsst
0 4 S593-p tPsVKKRsstLsQLP
0 6 S594-p PsVKKRsstLsQLPG
0 12 T595-p sVKKRsstLsQLPGD
0 3 S597-p KKRsstLsQLPGDKS
0 1 S620-p ETEASLAsRREQKRE
0 1 K653-u FGWSLPQkyKQVTNG
0 2 Y654-p GWSLPQkyKQVTNGQ
0 14 S705-p GKTRDGGsVVGASVF
0 1 Y713-p VVGASVFyKDVAGLD
0 6 S728-p TEGSKQRsAsQssLD
0 22 S730-p GSKQRsAsQssLDKL
0 10 S732-p KQRsAsQssLDKLDQ
0 29 S733-p QRsAsQssLDKLDQE
0 1 K742-a DKLDQELkEQQkELk
0 1 K746-a QELkEQQkELkNQEE
0 1 K749-a kEQQkELkNQEELSS
0 1 T804 SVPGARETDYPAGED
0 1 Y806 PGARETDYPAGEDLS
0 1 S815 AGEDLSESGQVDKAS
0 2 Y914-p DISQTGVyTEHVFTD
0 14 S1144-p GTGKLGFsFVRITAL
0 3 K1227-u HGHRDAVkFFVAVPG
0 1 S1238 AVPGQVISPQSSSSG
0 27 T1264-p AQEPGSQtPLKSMLV
0 1 S1290-p MGDEGGEsELLGEDL
0 1 K1305-u PLEPSVTkAERSHLI
  JIP4 iso2  
D5 ___MELEDGVVYQEE
Y9 ELEDGVVYQEEPGGS
S109 KFIEFEDSQEQEKKD
K178 MEHLERTKLHQLSGS
S183 RTKLHQLSGSDQLES
S185 KLHQLSGSDQLESTA
S194 QLESTAHSRIRKERP
K198 TAHSRIRKERPISLG
S203 IRKERPISLGIFPLP
T217-p PAGDGLLtPDAQKGG
A220 DGLLtPDAQKGGETP
T226 DAQKGGETPGSEQWK
S229 KGGETPGSEQWKFQE
K233 TPGSEQWKFQELSQP
S238 QWKFQELSQPRsHts
S242-p QELSQPRsHtsLKDE
T244-p LSQPRsHtsLKDELs
S245-p SQPRsHtsLKDELsD
- gap
S251-p tsLKDELsDVsQGGs
S254-p KDELsDVsQGGsKAT
S258-p sDVsQGGsKATTPAs
T261 sQGGsKATTPAsTAN
S265-p sKATTPAsTANsDVA
S269-p TPAsTANsDVAtIPT
T273-p TANsDVAtIPTDTPL
T276 sDVAtIPTDTPLKEE
T278 VAtIPTDTPLKEENE
S297 VTDAPNKSEISKHIE
S315-p AQETRNVstGsAENE
T316-p QETRNVstGsAENEE
S318-p TRNVstGsAENEEKS
S325 sAENEEKSEVQAIIE
S333 EVQAIIESTPELDMD
T334 VQAIIESTPELDMDK
S344 LDMDKDLSGYKGSSt
Y346 MDKDLSGYKGSStPT
S349 DLSGYKGSStPTKGI
T351-p SGYKGSStPTKGIEN
T353 YKGSStPTKGIENKA
T404 ENLILENTQLLETKN
R491 AQRKRFTRVEMARVL
S524 WTEMIRASRENPAMQ
Y568 EPPVNLKYNAPTSHV
T576 NAPTSHVTPSVKKRS
S578 PTSHVTPSVKKRSST
S583 TPSVKKRSSTLSQLP
S584 PSVKKRSSTLSQLPG
T585 SVKKRSSTLSQLPGD
S587 KKRSSTLSQLPGDKS
S610 ETEASLASRREQKRE
K643 FGWSLPQKYKQVTNG
Y644 GWSLPQKYKQVTNGQ
S695 GKTRDGGSVVGASVF
Y703 VVGASVFYKDVAGLD
S718 TEGSKQRSASQSSLD
S720 GSKQRSASQSSLDKL
S722 KQRSASQSSLDKLDQ
S723 QRSASQSSLDKLDQE
K732 DKLDQELKEQQKELK
K736 QELKEQQKELKNQEE
K739 KEQQKELKNQEELSS
T794 SVPGARETDYPAGED
Y796 PGARETDYPAGEDLS
S805 AGEDLSESGQVDKAS
Y904 DISQTGVYTEHVFTD
S1134 GTGKLGFSFVRITAL
K1217 HGHRDAVKFFVAVPG
S1228 AVPGQVISPQSSSSG
T1254 AQEPGSQTPLKSMLV
S1280 MGDEGGESELLGEDL
K1295 PLEPSVTKAERSHLI
  mouse

► Hide Isoforms 		 
D5 ___MELEDGVVYQEE
Y9 ELEDGVVYQEEPGGS
S109 KFIEFEDSQEQEKKD
K178-u MEHLERTkLHQLsGs
S183-p RTkLHQLsGsDQLEA
S185-p kLHQLsGsDQLEATA
S194 QLEATAHSRIRkERP
K198-u TAHSRIRkERPIsLG
S203-p IRkERPIsLGIFPLP
T217-p PAGDGLLtPDtQKGG
T220-p DGLLtPDtQKGGEtP
T226-p DtQKGGEtPGSEQWk
S229 KGGEtPGSEQWkFQE
K233-u tPGSEQWkFQELSQP
S238 QWkFQELSQPRSHTS
S242 QELSQPRSHTSLKVS
T244 LSQPRSHTSLKVSHS
S245 SQPRSHTSLKVSHSP
S251 TSLKVSHSPEPPKAV
S265 VEQEDELSDISQGGs
S268 EDELSDISQGGsKAT
S272-p SDISQGGsKATTPAS
T275 SQGGsKATTPASTAN
S279 sKATTPASTANSDVS
S283 TPASTANSDVSAIPP
A287 TANSDVSAIPPDtPS
P290 SDVSAIPPDtPSKED
T292-p VSAIPPDtPSKEDNE
S311 GTDTSNKSEISKHIE
S329-p AQETRNVsTEsGENE
T330 QETRNVsTEsGENEE
S332-p TRNVsTEsGENEEKS
S339 sGENEEKSEVQAIIE
S347 EVQAIIEStPELDMD
T348-p VQAIIEStPELDMDK
S358-p LDMDKDLsGyKGsSt
Y360-p MDKDLsGyKGsStPT
S363-p DLsGyKGsStPTKGI
T365-p sGyKGsStPTKGIEN
T367 yKGsStPTKGIENKA
T418 ENLILENTQLLETKN
R505-m1 AQRKRFTrVEMARVL
S538 WTEMIRASRENPAMQ
Y578 EPPVNLKYNAPTSHV
T586 NAPTSHVTPSVKKRs
S588 PTSHVTPSVKKRssT
S593-p TPSVKKRssTLSQLP
S594-p PSVKKRssTLSQLPG
T595 SVKKRssTLSQLPGD
S597 KKRssTLSQLPGDKS
S620 ETEASLASRREQKRE
K653 FGWSLPQKYKQVANG
Y654 GWSLPQKYKQVANGQ
S705-p GKTRDGGsVVGASVF
Y713 VVGASVFYKDIAGLD
S728-p TEGSKQRsAsQssLD
S730-p GSKQRsAsQssLDKL
S732-p KQRsAsQssLDKLDQ
S733-p QRsAsQssLDKLDQE
K742 DKLDQELKEQQKEFK
K746 QELKEQQKEFKNQEE
K749 KEQQKEFKNQEELSS
T804-p SVPGAREtDyPAGEE
Y806-p PGAREtDyPAGEELS
S815-p AGEELSEsGQVDKAS
Y914 DISQPGVYTEHVFTD
S1144 GTGKLGFSFVRITAL
K1227-u HGHRDAVkFFVAVPG
S1238-p AVPGQVIsPQSSSGG
T1264 AQEPSSQTPLKSMLV
S1290 MGDEGGESELLGEDL
K1305 PLEPSVTKAERSHLI
  JIP4 iso2  
D5 ___MELEDGVVYQEE
Y9 ELEDGVVYQEEPGGS
S109 KFIEFEDSQEQEKKD
K178 MEHLERTKLHQLSGS
S183 RTKLHQLSGSDQLEA
S185 KLHQLSGSDQLEATA
S194 QLEATAHSRIRKERP
K198 TAHSRIRKERPISLG
S203 IRKERPISLGIFPLP
T217 PAGDGLLTPDTQKGG
T220 DGLLTPDTQKGGETP
T226 DTQKGGETPGSEQWK
S229 KGGETPGSEQWKFQE
K233 TPGSEQWKFQELSQP
S238 QWKFQELSQPRSHTS
S242 QELSQPRSHTSLKDE
T244 LSQPRSHTSLKDELS
S245 SQPRSHTSLKDELSD
- gap
S251 TSLKDELSDISQGGS
S254 KDELSDISQGGSKAT
S258 SDISQGGSKATTPAS
T261 SQGGSKATTPASTAN
S265 SKATTPASTANSDVS
S269 TPASTANSDVSAIPP
A273 TANSDVSAIPPDTPS
P276 SDVSAIPPDTPSKED
T278 VSAIPPDTPSKEDNE
S297 GTDTSNKSEISKHIE
S315 AQETRNVSTESGENE
T316 QETRNVSTESGENEE
S318 TRNVSTESGENEEKS
S325 SGENEEKSEVQAIIE
S333 EVQAIIESTPELDMD
T334 VQAIIESTPELDMDK
S344 LDMDKDLSGYKGSST
Y346 MDKDLSGYKGSSTPT
S349 DLSGYKGSSTPTKGI
T351 SGYKGSSTPTKGIEN
T353 YKGSSTPTKGIENKA
T404 ENLILENTQLLETKN
R491 AQRKRFTRVEMARVL
S524 WTEMIRASRENPAMQ
Y564 EPPVNLKYNAPTSHV
T572 NAPTSHVTPSVKKRS
S574 PTSHVTPSVKKRSST
S579 TPSVKKRSSTLSQLP
S580 PSVKKRSSTLSQLPG
T581 SVKKRSSTLSQLPGD
S583 KKRSSTLSQLPGDKS
S606 ETEASLASRREQKRE
K639 FGWSLPQKYKQVANG
Y640 GWSLPQKYKQVANGQ
S691 GKTRDGGSVVGASVF
Y699 VVGASVFYKDIAGLD
S714 TEGSKQRSASQSSLD
S716 GSKQRSASQSSLDKL
S718 KQRSASQSSLDKLDQ
S719 QRSASQSSLDKLDQE
K728 DKLDQELKEQQKEFK
K732 QELKEQQKEFKNQEE
K735 KEQQKEFKNQEELSS
T790 SVPGARETDYPAGEE
Y792 PGARETDYPAGEELS
S801 AGEELSESGQVDKAS
Y900 DISQPGVYTEHVFTD
S1130 GTGKLGFSFVRITAL
K1213 HGHRDAVKFFVAVPG
S1224 AVPGQVISPQSSSGG
T1250 AQEPSSQTPLKSMLV
S1276 MGDEGGESELLGEDL
K1291 PLEPSVTKAERSHLI
  JIP4 iso3  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
K55-u NLPRILRkERPISLG
S60 LRkERPISLGIFPLP
T74 PAGDGLLTPDTQKGG
T77 DGLLTPDTQKGGETP
T83 DTQKGGETPGSEQWK
S86 KGGETPGSEQWKFQE
K90 TPGSEQWKFQELSQP
S95 QWKFQELSQPRSHTS
S99 QELSQPRSHTSLKDE
T101 LSQPRSHTSLKDELS
S102 SQPRSHTSLKDELSD
- gap
S108 TSLKDELSDISQGGS
S111 KDELSDISQGGSKAT
S115 SDISQGGSKATTPAS
T118 SQGGSKATTPASTAN
S122 SKATTPASTANSDVS
S126 TPASTANSDVSAIPP
A130 TANSDVSAIPPDTPS
P133 SDVSAIPPDTPSKED
T135 VSAIPPDTPSKEDNE
S154 GTDTSNKSEISKHIE
S172 AQETRNVSTESGENE
T173 QETRNVSTESGENEE
S175 TRNVSTESGENEEKS
S182 SGENEEKSEVQAIIE
S190 EVQAIIESTPELDMD
T191 VQAIIESTPELDMDK
S201 LDMDKDLSGYKGSST
Y203 MDKDLSGYKGSSTPT
S206 DLSGYKGSSTPTKGI
T208 SGYKGSSTPTKGIEN
T210 YKGSSTPTKGIENKA
T265 ENLILENTQLLETKN
R352 AQRKRFTRVEMARVL
S385 WTEMIRASRENPAMQ
Y425 EPPVNLKYNAPTSHV
T433 NAPTSHVTPSVKKRS
S435 PTSHVTPSVKKRSST
S440 TPSVKKRSSTLSQLP
S441 PSVKKRSSTLSQLPG
T442 SVKKRSSTLSQLPGD
S444 KKRSSTLSQLPGDKS
S467 ETEASLASRREQKRE
K500 FGWSLPQKYKQVANG
Y501 GWSLPQKYKQVANGQ
S552 GKTRDGGSVVGASVF
Y560 VVGASVFYKDIAGLD
S575 TEGSKQRSASQSSLD
S577 GSKQRSASQSSLDKL
S579 KQRSASQSSLDKLDQ
S580 QRSASQSSLDKLDQE
K589 DKLDQELKEQQKEFK
K593 QELKEQQKEFKNQEE
K596 KEQQKEFKNQEELSS
T651 SVPGARETDYPAGEE
Y653 PGARETDYPAGEELS
S662 AGEELSESGQVDKAS
Y761 DISQPGVYTEHVFTD
S991 GTGKLGFSFVRITAL
K1074 HGHRDAVKFFVAVPG
S1085 AVPGQVISPQSSSGG
T1111 AQEPSSQTPLKSMLV
S1137 MGDEGGESELLGEDL
K1152 PLEPSVTKAERSHLI
  rat

 
D5 ___MELEDGVVYQEE
Y9 ELEDGVVYQEEPGGS
S109 KFIEFEDSQEQEKKD
K178 MEHLERTKLHQLSGS
S183 RTKLHQLSGSEQLEA
S185 KLHQLSGSEQLEATA
S194 QLEATAHSRIRKERP
K198 TAHSRIRKERPISLG
S203 IRKERPISLGIFPLP
T217 PAGDGLLTPDTQKGG
T220 DGLLTPDTQKGGETP
T226 DTQKGGETPGSEQWK
S229 KGGETPGSEQWKFQE
K233 TPGSEQWKFQELSQP
S238 QWKFQELSQPRsHTS
S242-p QELSQPRsHTSLKDE
T244 LSQPRsHTSLKDELS
S245 SQPRsHTSLKDELSD
- gap
S251 TSLKDELSDISQGGS
S254 KDELSDISQGGSKAT
S258 SDISQGGSKATTPAS
T261 SQGGSKATTPASTAN
S265 SKATTPASTANSDVS
S269 TPASTANSDVSAIPP
A273 TANSDVSAIPPDTPS
P276 SDVSAIPPDTPSKED
T278 VSAIPPDTPSKEDNE
A297 GTDTPNKAEISKHIE
S315 AQETRNVSTDSAENE
T316 QETRNVSTDSAENEE
S318 TRNVSTDSAENEEKS
S325 SAENEEKSEVQAIIE
S333 EVQAIIESTPELDMD
T334 VQAIIESTPELDMDK
S344 LDMDKDLSGYKGSST
Y346 MDKDLSGYKGSSTPT
S349 DLSGYKGSSTPTKGI
T351 SGYKGSSTPTKGIEN
T353 YKGSSTPTKGIENKA
T404 ENLILENTQLLETKN
R491 AQRKRFTRVEMARVL
S524 WTEMIRASRENPAMQ
Y564 EPPVNLKYNAPTSHV
T572 NAPTSHVTPSVKKRS
S574 PTSHVTPSVKKRSST
S579 TPSVKKRSSTLSQLP
S580 PSVKKRSSTLSQLPG
T581 SVKKRSSTLSQLPGD
S583 KKRSSTLSQLPGDKS
S606 ETEASLASRREQKRE
K639 FGWSLPQKYKQVANG
Y640 GWSLPQKYKQVANGQ
S691 GKTRDGGSVVGASVF
Y699 VVGASVFYKDVSALE
S714-p SEGSKQRsAsQssLD
S716-p GSKQRsAsQssLDKL
S718-p KQRsAsQssLDKLDQ
S719-p QRsAsQssLDKLDQE
K728 DKLDQELKEQQKELK
K732 QELKEQQKELKNQEE
K735 KEQQKELKNQEELSS
T790 SVPGARETDYPAGEE
Y792 PGARETDYPAGEELS
S801 AGEELSDSGQVDRAS
Y900 DISQPGVYTEHVFTD
S1130 GTGKLGFSFVRITAL
K1226 HGHRDAVKFFVAVPG
S1237 AVPGQVVSPQSSSSG
T1263 AQEPSSQTPLKSMLV
S1289 MGDEGGESELLGEDL
K1304 PLEPSVTKAERSHLI
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.