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Protein Page:
CHD-4 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
CHD-4 is an ATP-dependent helicase and a central component of the HDAC-containing nucleosome remodeling and histone deacetylase (NuRD) transcriptional complex. Interacts with MTA1/2, HDAC1, and RbAp46 in the NuRD complex. CHD-4 belongs to the SNF2/RAD54 helicase family of proteins. The NuRD complex, whose subunit composition varies by cell type and in response to physiologic signals, plays central roles in many processes including oncogenesis and metastasis, B cell differentiation, invasive growth of breast cancer, and maintaining the balance between the undifferentiated state and the process of differentiation in embryonic stem cells. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Helicase; EC 3.6.1.-; EC 3.6.4.12
Cellular Component: centrosome; cytoplasm; NuRD complex; nucleus
Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; zinc ion binding; transcription factor binding; ATP binding
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; chromatin modification
Reference #:  Q14839 (UniProtKB)
Alt. Names/Synonyms: ATP-dependent helicase CHD4; CHD-4; CHD4; chromodomain helicase DNA binding protein 4; Chromodomain-helicase-DNA-binding protein 4; DKFZp686E06161; Mi-2 autoantigen 218 kDa protein; Mi-2b; Mi2-beta
Gene Symbols: CHD4
Molecular weight: 218,005 Da
Basal Isoelectric point: 5.62  Predict pI for various phosphorylation states
CST Pathways:  Protein Acetylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

CHD-4
Protein Structure Not Found.


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Sites Implicated In
intracellular localization: S1349‑p
molecular association, regulation: S1349‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

 
0 1 S27-p MDALLNNsLPPPHPE
0 9 S44-p EDPEEDLsEtETPKL
0 1 T46-p PEEDLsEtETPKLKK
0 1 S85 LCRQLGDSsGEGPEF
0 1 S86-p CRQLGDSsGEGPEFV
0 5 S103-p EEEVALRsDsEGsDy
0 3 S105-p EVALRsDsEGsDyTP
0 4 S108-p LRsDsEGsDyTPGKk
0 2 Y110-p sDsEGsDyTPGKkkK
0 1 T111 DsEGsDyTPGKkkKk
0 1 K115-a sDyTPGKkkKkkLGP
0 1 K116-a DyTPGKkkKkkLGPk
0 2 K118-a TPGKkkKkkLGPkKE
0 1 K119-a PGKkkKkkLGPkKEK
0 3 K123-a kKkkLGPkKEKKSKS
0 1 K273-a KGPNARRkPkGsPRV
0 1 K275-a PNARRkPkGsPRVPD
0 2 S277-p ARRkPkGsPRVPDAK
0 1 K285-a PRVPDAKkPKPKKVA
0 1 S303-p IKLGGFGskRKRsss
0 67 K304-a KLGGFGskRKRsssE
0 11 S308-p FGskRKRsssEDDDL
0 12 S309-p GskRKRsssEDDDLD
0 12 S310-p skRKRsssEDDDLDV
0 14 S319-p DDDLDVEsDFDDAsI
0 2 S325-p EsDFDDAsINSYSVS
0 1 S330 DAsINSYSVSDGSTS
0 1 T360-p KKGEEEVtAVDGyEt
0 1 Y365-p EVtAVDGyEtDHQDy
0 8 T367-p tAVDGyEtDHQDyCE
0 1 Y372-p yEtDHQDyCEVCQQG
0 19 S428-p WEAKEDNsEGEEILE
0 1 K504-u ALKGKVQkILIWKWG
0 31 S515-p WKWGQPPsPtPVPRP
0 12 T517-p WGQPPsPtPVPRPPD
1 30 T529-p PPDADPNtPsPKPLE
1 31 S531-p DADPNtPsPKPLEGR
0 2 T705-p ERPPETPtVDPtVKY
0 1 T709-p ETPtVDPtVKYERQP
0 1 K833 NAIRGGKKASRMKKE
0 2 Y985 LSPMQKKYYKYILTR
0 1 Y986 SPMQKKYYKYILTRN
0 6 K1016-a LNVVMDLkkCCNHPY
0 1 K1016-u LNVVMDLkkCCNHPY
0 2 K1017-a NVVMDLkkCCNHPYL
0 2 K1057-u GKLLLLQkMLKNLKE
0 50 K1092-u FLEHEGYkYERIDGG
0 1 S1209 GLGSKTGSMSKQELD
0 2 D1233-ca LFKDEATdGGGDNKE
0 1 K1252-u SVIHYDDkAIERLLD
0 5 Y1276-p ELQGMNEyLSSFKVA
0 1 K1304-u EVEREIIkQEEsVDP
0 5 S1308-p EIIkQEEsVDPDYWE
1 5 S1349-p QVNYNDGsQEDRDWQ
0 3 S1360 RDWQDDQSDNQSDYS
0 1 S1364 DDQSDNQSDYSVAsE
0 5 S1367 SDNQSDYSVAsEEGD
0 7 S1370-p QSDYSVAsEEGDEDF
0 1 K1390-u APRRPSRkGLRNDkD
0 1 K1396-u RkGLRNDkDKPLPPL
0 2 S1500-p LTRIGVMsLIRKKVQ
0 22 S1531-p VEENKKMsQPGsPsP
0 42 S1535-p KKMsQPGsPsPKtPt
0 14 S1537-p MsQPGsPsPKtPtPs
0 27 T1540-p PGsPsPKtPtPstPG
0 20 T1542-p sPsPKtPtPstPGDt
0 7 S1544-p sPKtPtPstPGDtQP
0 42 T1545-p PKtPtPstPGDtQPN
0 25 T1549-p tPstPGDtQPNtPAP
0 50 T1553-p PGDtQPNtPAPVPPA
0 3 S1570-p GIKIEENsLkEEEsI
0 2 K1572-u KIEENsLkEEEsIEG
0 2 S1576-p NsLkEEEsIEGEKEV
0 1 K1584-u IEGEKEVkSTAPETA
0 1 T1595-p PETAIECtQAPAPAs
0 3 S1602-p tQAPAPAsEDEKVVV
0 1 K1643-a KGAADVEkVEEkSAI
0 1 K1647-u DVEkVEEkSAIDLtP
0 3 T1653-p EkSAIDLtPIVVEDK
0 2 T1679-p VMLQNGEtPKDLNDE
0 1 T1722-p EERAATVtKKtYEIW
0 1 T1725-p AATVtKKtYEIWHRR
0 1 Y1804-p EQLRRAAyLNMsEDP
0 1 S1808-p RAAyLNMsEDPSHPS
0 1 S1839-p HQHLSKEsMAGNKPA
0 1 K1852-u PANAVLHkVLkQLEE
0 1 K1855-u AVLHkVLkQLEELLS
0 4 K1865-u EELLSDMkADVTRLP
0 3 T1904-p ANRAPEPtPQQVAQQ
  mouse

 
S27 MDALLNNSLPPPHPE
S44-p EDPDEDLsEAETPKL
A46 PDEDLsEAETPKLKK
S78-p QKKELGDsSGEGPEF
S79 KKELGDsSGEGPEFV
S96-p EEEVALRsDsEGsDy
S98-p EVALRsDsEGsDytP
S101-p LRsDsEGsDytPGKK
Y103-p sDsEGsDytPGKKKK
T104-p DsEGsDytPGKKKKK
K108 sDytPGKKKKKKLGP
K109 DytPGKKKKKKLGPK
K111 tPGKKKKKKLGPKKE
K112 PGKKKKKKLGPKKEK
K116 KKKKLGPKKEKKSKS
K266 KGPNARRKPKGsPRV
K268 PNARRKPKGsPRVPD
S270-p ARRKPKGsPRVPDAK
K278 PRVPDAKKPKPKKVA
S296 IKLGGFGSkRKRsss
K297-a KLGGFGSkRKRsssE
S301-p FGSkRKRsssEDDDL
S302-p GSkRKRsssEDDDLD
S303-p SkRKRsssEDDDLDV
S312-p DDDLDVEsDFDDAsI
S318-p EsDFDDAsINSYsVS
S323-p DAsINSYsVSDGSTS
T353 KKGEEEVTAVDGYET
Y358 EVTAVDGYETDHQDY
T360 TAVDGYETDHQDYCE
Y365 YETDHQDYCEVCQQG
S421-p WEAKEDNsEGEEILE
K497 ALKGKVQKILIWKWG
S508-p WKWGQPPsPtPVPRP
T510-p WGQPPsPtPVPRPPD
T522-p PPDADPNtPsPKPLE
S524-p DADPNtPsPKPLEGR
T698-p ERPPETPtVDPTVKY
T702 ETPtVDPTVKYERQP
K826-a NAIRGGKkASRMKKE
Y978-p LSPMQKKyYKYILTR
Y979 SPMQKKyYKYILTRN
K1009-a LNVVMDLkKCCNHPY
K1009 LNVVMDLKKCCNHPY
K1010 NVVMDLkKCCNHPYL
K1050-u GKLLLLQkMLKNLKE
K1085-u FLEHEGYkYERIDGG
S1202-p GLGSKTGsMSKQELD
D1226 LFKDEATDGGGDNKE
K1245 SVIHYDDKAIERLLD
Y1269 ELQGMNEYLSSFKVA
K1297 EVEREIIKQEEsVDP
S1301-p EIIKQEEsVDPDYWE
S1342-p QVNYNDGsQEDRDWQ
S1353-p RDWQDDQsDNQsDYs
S1357-p DDQsDNQsDYsVAsE
S1360-p sDNQsDYsVAsEEGD
S1363-p QsDYsVAsEEGDEDF
K1383 APRRPSRKGLRNDKD
K1389 RKGLRNDKDKPLPPL
S1493 LTRIGVMSLIRKKVQ
S1524-p VEENKKMsQPGsPsP
S1528-p KKMsQPGsPsPKtPt
S1530-p MsQPGsPsPKtPtPs
T1533-p PGsPsPKtPtPstPG
T1535-p sPsPKtPtPstPGDt
S1537-p sPKtPtPstPGDtQP
T1538-p PKtPtPstPGDtQPN
T1542-p tPstPGDtQPNtPAP
T1546-p PGDtQPNtPAPVPPA
S1563-p GIKIEENsLkEEEST
K1565-u KIEENsLkEEESTEG
S1569 NsLkEEESTEGEKEV
K1577 TEGEKEVKSTAPEAT
A1588 PEATVECAQPPAPAP
P1595 AQPPAPAPATAPATA
K1646 KGTTEVEKVEEKSAV
K1650 EVEKVEEKSAVDLTP
T1656 EKSAVDLTPIVVEDK
T1682-p VMLQNGEtPKDLSDE
T1725 EERAATVTKKTYEIW
T1728 AATVTKKTYEIWHRR
Y1807 EQLRRAAYLNMSEDP
S1811 RAAYLNMSEDPSHPS
S1842 HQHLSKESMAGNKPA
K1855 PANAVLHKVLKQLEE
K1858 AVLHKVLKQLEELLS
K1868-u EELLSDMkADVTRLP
P1907 ANRAPEPPPQQVAQQ
  rat

 
S27 MDALLNNSLPPPHPE
S44 DDPEEDLSEAETPKL
A46 PEEDLSEAETPKLKK
S78 QKKELGDSSGEGPEF
S79 KKELGDSSGEGPEFV
S96 DEEAALRSDSEGSDY
S98 EAALRSDSEGSDYTP
S101 LRSDSEGSDYTPGKK
Y103 SDSEGSDYTPGKKKK
T104 DSEGSDYTPGKKKKK
K108 SDYTPGKKKKKKLGP
K109 DYTPGKKKKKKLGPK
K111 TPGKKKKKKLGPKKE
K112 PGKKKKKKLGPKKEK
K116 KKKKLGPKKEKKSKS
K266 KGPNARRKPKGSPRV
K268 PNARRKPKGSPRVPD
S270 ARRKPKGSPRVPDAK
K278 PRVPDAKKPKPKKVA
S296 IKLGGFGSKRKRSSS
K297 KLGGFGSKRKRSSSE
S301 FGSKRKRSSSEDDDL
S302 GSKRKRSSSEDDDLD
S303 SKRKRSSSEDDDLDV
S312 DDDLDVESDFDDASI
S318 ESDFDDASINSYSVS
S323 DASINSYSVSDGSTS
T353 KKGEEEVTAVDGYET
Y358 EVTAVDGYETDHQDY
T360 TAVDGYETDHQDYCE
Y365 YETDHQDYCEVCQQG
S421 WEAKEDNSEGEEILE
K497 ALKGKVQKILIWKWG
S508 WKWGQPPSPTPVPRP
T510 WGQPPSPTPVPRPPD
T522 PPDADPNTPSPKPLE
S524 DADPNTPSPKPLEGR
T698 ERPPETPTVDPTVKY
T702 ETPTVDPTVKYERQP
K826 NAIRGGKKASRMKKE
Y978-p LSPMQKKyyKYILTR
Y979-p SPMQKKyyKYILTRN
K1009 LNVVMDLKKCCNHPY
K1009 LNVVMDLKKCCNHPY
K1010 NVVMDLKKCCNHPYL
K1050 GKLLLLQKMLKNLKE
K1085 FLEHEGYKYERIDGG
S1202 GLGSKTGSMSKQELD
D1226 LFKDEATDGGGDNKE
K1245 SVIHYDDKAIERLLD
Y1269 ELQGMNEYLSSFKVA
K1297 EVEREIIKQEESVDP
S1301 EIIKQEESVDPDYWE
S1342 QVNYNDGSQEDRDWQ
S1353 RDWQDDQSDNQSDYS
S1357 DDQSDNQSDYSVASE
S1360 SDNQSDYSVASEEGD
S1363 QSDYSVASEEGDEDF
K1383 APRRPSRKGLRNDKD
K1389 RKGLRNDKDKPLPPL
S1493 LTRIGVMSLIRKKVQ
S1524 VEENKKMSQPGsPSP
S1528-p KKMSQPGsPSPKTPT
S1530 MSQPGsPSPKTPTPS
T1533 PGsPSPKTPTPSTPG
T1535 sPSPKTPTPSTPGDT
S1537 SPKTPTPSTPGDTQP
T1538 PKTPTPSTPGDTQPN
T1542 TPSTPGDTQPNTPAP
T1546 PGDTQPNTPAPVPPA
S1563 GIKIEENSLKEEEST
K1565 KIEENSLKEEESTEG
S1569 NSLKEEESTEGEKEV
K1577 TEGEKEVKPTAPEVT
A1588 PEVTVECAQPPAPAP
P1611 TPGPAPAPEDDKAPA
K1652 KGTTEVEKAEEKSAV
K1656 EVEKAEEKSAVDLTP
T1662 EKSAVDLTPIVVEDK
T1688 VLLQNGETPKDLSDE
T1731 EERAATVTKKTYEIW
T1734 AATVTKKTYEIWHRR
Y1813 EQLRRAAYLNMSEDP
S1817 RAAYLNMSEDPSHPS
S1848 HQHLSKESMAGNKPA
K1861 PANAVLHKVLKQLEE
K1864 AVLHKVLKQLEELLS
K1874 EELLSDMKADVTRLP
P1913 ANRAPEPPPQQVAQQ
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