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Protein Page:
CHD-4 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
CHD-4 is an ATP-dependent helicase and a central component of the HDAC-containing nucleosome remodeling and histone deacetylase (NuRD) transcriptional complex. Interacts with MTA1/2, HDAC1, and RbAp46 in the NuRD complex. CHD-4 belongs to the SNF2/RAD54 helicase family of proteins. The NuRD complex, whose subunit composition varies by cell type and in response to physiologic signals, plays central roles in many processes including oncogenesis and metastasis, B cell differentiation, invasive growth of breast cancer, and maintaining the balance between the undifferentiated state and the process of differentiation in embryonic stem cells. Note: This description may include information from UniProtKB.
Protein type: EC 3.6.1.-; EC 3.6.4.12; DNA binding protein; Helicase
Cellular Component: nucleoplasm; centrosome; protein complex; cytoplasm; nuclear chromatin; NuRD complex
Molecular Function: ATP-dependent DNA helicase activity; protein binding; nucleosomal DNA binding; DNA binding; zinc ion binding; ATP binding
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; ATP-dependent chromatin remodeling; DNA duplex unwinding
Reference #:  Q14839 (UniProtKB)
Alt. Names/Synonyms: ATP-dependent helicase CHD4; CHD-4; CHD4; chromodomain helicase DNA binding protein 4; Chromodomain-helicase-DNA-binding protein 4; DKFZp686E06161; Mi-2 autoantigen 218 kDa protein; Mi-2b; Mi2-beta
Gene Symbols: CHD4
Molecular weight: 218,005 Da
Basal Isoelectric point: 5.62  Predict pI for various phosphorylation states
CST Pathways:  Protein Acetylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

CHD-4

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Source  |  InnateDB  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein


Sites Implicated In
intracellular localization: S1349‑p
molecular association, regulation: S1349‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 9 S44-p EDPEEDLsETETPKL
0 1 S85 LCRQLGDSsGEGPEF
0 1 S86-p CRQLGDSsGEGPEFV
0 5 S103-p EEEVALRsDsEGsDy
0 3 S105-p EVALRsDsEGsDyTP
0 5 S108-p LRsDsEGsDyTPGKK
0 3 Y110-p sDsEGsDyTPGKKKK
0 2 T111 DsEGsDyTPGKKKKk
0 2 K118-a TPGKKKKkKLGPkKE
0 3 K123-a KKkKLGPkKEKKSKS
0 3 S277-p ARRKPKGsPRVPDAK
0 2 S303-p IKLGGFGskRKRsss
0 67 K304-a KLGGFGskRKRsssE
0 14 S308-p FGskRKRsssEDDDL
0 15 S309-p GskRKRsssEDDDLD
0 16 S310-p skRKRsssEDDDLDV
0 17 S319-p DDDLDVEsDFDDAsI
0 3 S325-p EsDFDDAsINSySVS
0 1 S328 FDDAsINSySVSDGS
0 1 Y329-p DDAsINSySVSDGST
0 1 S330 DAsINSySVSDGSTS
0 8 T367-p TAVDGYEtDHQDYCE
0 22 S428-p WEAKEDNsEGEEILE
0 1 K504-u ALKGKVQkILIWKWG
0 34 S515-p WKWGQPPsPtPVPRP
0 13 T517-p WGQPPsPtPVPRPPD
1 32 T529-p PPDADPNtPsPKPLE
1 34 S531-p DADPNtPsPKPLEGR
0 1 T703-p KLERPPEtPtVDPTV
0 2 T705-p ERPPEtPtVDPTVKY
0 1 T761 GLGKTVQTAVFLYSL
0 1 Y766 VQTAVFLYSLYKEGH
0 1 S767 QTAVFLYSLYKEGHS
0 1 Y769 AVFLYSLYKEGHSKG
0 2 Y985 LSPMQKKYYKYILTR
0 6 K1016-a LNVVMDLkkCCNHPY
0 1 K1016-u LNVVMDLkkCCNHPY
0 2 K1017-a NVVMDLkkCCNHPYL
0 2 K1057-u GKLLLLQkMLKNLKE
0 50 K1092-u FLEHEGYkYERIDGG
0 1 T1207-p RPGLGSKtGSMSKQE
0 1 S1209 GLGSKtGSMSKQELD
0 2 D1233-ca LFKDEATdGGGDNKE
0 1 K1252-u SVIHYDDkAIERLLD
0 5 Y1276-p ELQGMNEyLSSFKVA
0 1 K1304-u EVEREIIkQEEsVDP
0 6 S1308-p EIIkQEEsVDPDYWE
1 5 S1349-p QVNYNDGsQEDRDWQ
0 3 S1360 RDWQDDQSDNQSDYS
0 5 S1367 SDNQSDYSVAsEEGD
0 7 S1370-p QSDYSVAsEEGDEDF
0 2 S1500-p LTRIGVMsLIRKKVQ
0 27 S1531-p VEENKKMsQPGsPsP
0 48 S1535-p KKMsQPGsPsPKtPt
0 16 S1537-p MsQPGsPsPKtPtPs
0 27 T1540-p PGsPsPKtPtPstPG
0 20 T1542-p sPsPKtPtPstPGDt
0 8 S1544-p sPKtPtPstPGDtQP
0 42 T1545-p PKtPtPstPGDtQPN
0 27 T1549-p tPstPGDtQPNtPAP
0 52 T1553-p PGDtQPNtPAPVPPA
0 4 S1570-p GIKIEENsLkEEEsI
0 2 K1572-u KIEENsLkEEEsIEG
0 3 S1576-p NsLkEEEsIEGEKEV
0 1 K1584-u IEGEKEVkSTAPETA
0 4 S1602-p TQAPAPAsEDEKVVV
0 1 K1643-a KGAADVEkVEEksAI
0 1 K1647-u DVEkVEEksAIDLtP
0 1 S1648-p VEkVEEksAIDLtPI
0 4 T1653-p EksAIDLtPIVVEDK
0 3 T1679-p VMLQNGEtPKDLNDE
0 1 S1839-p HQHLSKEsMAGNKPA
0 1 K1852-u PANAVLHkVLkQLEE
0 1 K1855-u AVLHkVLkQLEELLS
0 4 K1865-u EELLSDMkADVTRLP
0 3 T1904-p ANRAPEPtPQQVAQQ
  CHD-4 iso4  
S44 EDPEEDLSETETPKL
S78 QKKELGDSSGEGPEF
S79 KKELGDSSGEGPEFV
S96 EEEVALRSDsEGsDy
S98-p EVALRSDsEGsDyTP
S101-p LRSDsEGsDyTPGKK
Y103-p SDsEGsDyTPGKKKK
T104 DsEGsDyTPGKKKKK
K111 TPGKKKKKKLGPKKE
K116 KKKKLGPKKEKKSKS
S270 ARRKPKGSPRVPDAK
S296 IKLGGFGSKRKRSSS
K297 KLGGFGSKRKRSSSE
S301 FGSKRKRSSSEDDDL
S302 GSKRKRSSSEDDDLD
S303 SKRKRSSSEDDDLDV
S312 DDDLDVESDFDDASI
S318 ESDFDDASINSYSVS
S321 FDDASINSYSVSDGS
Y322 DDASINSYSVSDGST
S323 DASINSYSVSDGSTS
T360 TAVDGYETDHQDYCE
S421 WEAKEDNSEGEEILE
K497 ALKGKVQKILIWKWG
S508 WKWGQPPSPTPVPRP
T510 WGQPPSPTPVPRPPD
T522 PPDADPNTPSPKPLE
S524 DADPNTPSPKPLEGR
T696 KLERPPETPTVDPTV
T698 ERPPETPTVDPTVKY
T754 GLGKTVQTAVFLYSL
Y759 VQTAVFLYSLYKEGH
S760 QTAVFLYSLYKEGHS
Y762 AVFLYSLYKEGHSKG
Y978 LSPMQKKYYKYILTR
K1009 LNVVMDLKKCCNHPY
K1009 LNVVMDLKKCCNHPY
K1010 NVVMDLKKCCNHPYL
K1050 GKLLLLQKMLKNLKE
K1085 FLEHEGYKYERIDGG
T1200 RPGLGSKTGSMSKQE
S1202 GLGSKTGSMSKQELD
D1226 LFKDEATDGGGDNKE
K1245 SVIHYDDKAIERLLD
Y1269 ELQGMNEYLSSFKVA
K1297 EVEREIIKQEESVDP
S1301 EIIKQEESVDPDYWE
S1342 QVNYNDGSQEDRDWQ
S1353 RDWQDDQSDNQSDYS
S1360 SDNQSDYSVASEEGD
S1363 QSDYSVASEEGDEDF
S1493 LTRIGVMSLIRKKVQ
S1524 VEENKKMSQPGSPSP
S1528 KKMSQPGSPSPKTPT
S1530 MSQPGSPSPKTPTPS
T1533 PGSPSPKTPTPSTPG
T1535 SPSPKTPTPSTPGDT
S1537 SPKTPTPSTPGDTQP
T1538 PKTPTPSTPGDTQPN
T1542 TPSTPGDTQPNTPAP
T1546 PGDTQPNTPAPVPPA
S1563 GIKIEENSLKEEESI
K1565 KIEENSLKEEESIEG
S1569 NSLKEEESIEGEKEV
K1577 IEGEKEVKSTAPETA
S1595 TQAPAPASEDEKVVV
K1636 KGAADVEKVEEKSAI
K1640 DVEKVEEKSAIDLTP
S1641 VEKVEEKSAIDLTPI
T1646 EKSAIDLTPIVVEDK
T1672 VMLQNGETPKDLNDE
S1832 HQHLSKESMAGNKPA
K1845 PANAVLHKVLKQLEE
K1848 AVLHKVLKQLEELLS
K1858 EELLSDMKADVTRLP
T1897 ANRAPEPTPQQVAQQ
  mouse

► Hide Isoforms
 
S44-p EDPDEDLsEAETPKL
S78-p QKKELGDsSGEGPEF
S79 KKELGDsSGEGPEFV
S96-p EEEVALRsDsEGsDy
S98-p EVALRsDsEGsDytP
S101-p LRsDsEGsDytPGKK
Y103-p sDsEGsDytPGKKKK
T104-p DsEGsDytPGKKKKK
K111 tPGKKKKKKLGPKKE
K116 KKKKLGPKKEKKSKS
S270-p ARRKPKGsPRVPDAK
S296 IKLGGFGSkRKRsss
K297-a KLGGFGSkRKRsssE
S301-p FGSkRKRsssEDDDL
S302-p GSkRKRsssEDDDLD
S303-p SkRKRsssEDDDLDV
S312-p DDDLDVEsDFDDAsI
S318-p EsDFDDAsINsysVS
S321-p FDDAsINsysVSDGS
Y322-p DDAsINsysVSDGST
S323-p DAsINsysVSDGSTS
T360 TAVDGYETDHQDYCE
S421-p WEAKEDNsEGEEILE
K497 ALKGKVQKILIWKWG
S508-p WKWGQPPsPtPVPRP
T510-p WGQPPsPtPVPRPPD
T522-p PPDADPNtPsPKPLE
S524-p DADPNtPsPKPLEGR
T696 KLERPPETPtVDPTV
T698-p ERPPETPtVDPTVKY
T754-p GLGKTVQtAVFLysL
Y759-p VQtAVFLysLyKEGH
S760-p QtAVFLysLyKEGHS
Y762-p AVFLysLyKEGHSKG
Y978-p LSPMQKKyYKYILTR
K1009-a LNVVMDLkKCCNHPY
K1009 LNVVMDLKKCCNHPY
K1010 NVVMDLkKCCNHPYL
K1050-u GKLLLLQkMLKNLKE
K1085-u FLEHEGYkYERIDGG
T1200-p RPGLGSKtGsMSKQE
S1202-p GLGSKtGsMSKQELD
D1226 LFKDEATDGGGDNKE
K1245 SVIHYDDKAIERLLD
Y1269 ELQGMNEYLSSFKVA
K1297 EVEREIIKQEEsVDP
S1301-p EIIKQEEsVDPDYWE
S1342-p QVNYNDGsQEDRDWQ
S1353-p RDWQDDQsDNQSDYs
S1360-p sDNQSDYsVAsEEGD
S1363-p QSDYsVAsEEGDEDF
S1493 LTRIGVMSLIRKKVQ
S1524-p VEENKKMsQPGsPsP
S1528-p KKMsQPGsPsPKtPt
S1530-p MsQPGsPsPKtPtPs
T1533-p PGsPsPKtPtPstPG
T1535-p sPsPKtPtPstPGDt
S1537-p sPKtPtPstPGDtQP
T1538-p PKtPtPstPGDtQPN
T1542-p tPstPGDtQPNtPAP
T1546-p PGDtQPNtPAPVPPA
S1563-p GIKIEENsLkEEEST
K1565-u KIEENsLkEEESTEG
S1569 NsLkEEESTEGEKEV
K1577 TEGEKEVKSTAPEAT
- gap
K1646 KGTTEVEKVEEKSAV
K1650 EVEKVEEKSAVDLTP
S1651 VEKVEEKSAVDLTPI
T1656 EKSAVDLTPIVVEDK
T1682-p VMLQNGEtPKDLSDE
S1842 HQHLSKESMAGNKPA
K1855 PANAVLHKVLKQLEE
K1858 AVLHKVLKQLEELLS
K1868-u EELLSDMkADVTRLP
P1907 ANRAPEPPPQQVAQQ
  CHD-4 iso3  
S44 EDPDEDLSEAETPKL
S85 LCRQLGDSSGEGPEF
S86 CRQLGDSSGEGPEFV
S103 EEEVALRSDSEGsDy
S105 EVALRSDSEGsDytP
S108-p LRSDSEGsDytPGKK
Y110-p SDSEGsDytPGKKKK
T111-p DSEGsDytPGKKKKK
K118 tPGKKKKKKLGPKKE
K123 KKKKLGPKKEKKSKS
S277 ARRKPKGSPRVPDAK
S303 IKLGGFGSKRKRSSS
K304 KLGGFGSKRKRSSSE
S308 FGSKRKRSSSEDDDL
S309 GSKRKRSSSEDDDLD
S310 SKRKRSSSEDDDLDV
S319 DDDLDVESDFDDASI
S325 ESDFDDASINSYSVS
S328 FDDASINSYSVSDGS
Y329 DDASINSYSVSDGST
S330 DASINSYSVSDGSTS
T367 TAVDGYETDHQDYCE
S428 WEAKEDNSEGEEILE
K504 ALKGKVQKILIWKWG
S515 WKWGQPPSPTPVPRP
T517 WGQPPSPTPVPRPPD
T529 PPDADPNTPSPKPLE
S531 DADPNTPSPKPLEGR
T703 KLERPPETPTVDPTV
T705 ERPPETPTVDPTVKY
T761 GLGKTVQTAVFLYSL
Y766 VQTAVFLYSLYKEGH
S767 QTAVFLYSLYKEGHS
Y769 AVFLYSLYKEGHSKG
Y985 LSPMQKKYYKYILTR
K1016 LNVVMDLKKCCNHPY
K1016 LNVVMDLKKCCNHPY
K1017 NVVMDLKKCCNHPYL
K1057 GKLLLLQKMLKNLKE
K1092 FLEHEGYKYERIDGG
T1207 RPGLGSKTGSMSKQE
S1209 GLGSKTGSMSKQELD
D1233 LFKDEATDGGGDNKE
K1252 SVIHYDDKAIERLLD
Y1276 ELQGMNEYLSSFKVA
K1304 EVEREIIKQEESVDP
S1308 EIIKQEESVDPDYWE
S1349 QVNYNDGSQEDRDWQ
S1360 RDWQDDQSDNQSDYS
S1367 SDNQSDYSVASEEGD
S1370 QSDYSVASEEGDEDF
S1500 LTRIGVMSLIRKKVQ
S1531 VEENKKMSQPGSPSP
S1535 KKMSQPGSPSPKTPT
S1537 MSQPGSPSPKTPTPS
T1540 PGSPSPKTPTPSTPG
T1542 SPSPKTPTPSTPGDT
S1544 SPKTPTPSTPGDTQP
T1545 PKTPTPSTPGDTQPN
T1549 TPSTPGDTQPNTPAP
T1553 PGDTQPNTPAPVPPA
S1570 GIKIEENSLKEEEST
K1572 KIEENSLKEEESTEG
S1576 NSLKEEESTEGEKEV
K1584 TEGEKEVKSTAPEAT
- gap
K1653 KGTTEVEKVEEKSAV
K1657 EVEKVEEKSAVDLTP
S1658 VEKVEEKSAVDLTPI
T1663 EKSAVDLTPIVVEDK
T1689 VMLQNGETPKDLSDE
S1849 HQHLSKESMAGNKPA
K1862 PANAVLHKVLKQLEE
K1865 AVLHKVLKQLEELLS
K1875 EELLSDMKADVTRLP
P1914 ANRAPEPPPQQVAQQ
  rat

 
S44 DDPEEDLSEAETPKL
S78 QKKELGDSSGEGPEF
S79 KKELGDSSGEGPEFV
S96 DEEAALRSDSEGSDY
S98 EAALRSDSEGSDYTP
S101 LRSDSEGSDYTPGKK
Y103 SDSEGSDYTPGKKKK
T104 DSEGSDYTPGKKKKK
K111 TPGKKKKKKLGPKKE
K116 KKKKLGPKKEKKSKS
S270 ARRKPKGSPRVPDAK
S296 IKLGGFGSKRKRSSs
K297 KLGGFGSKRKRSSsE
S301 FGSKRKRSSsEDDDL
S302 GSKRKRSSsEDDDLD
S303-p SKRKRSSsEDDDLDV
S312 DDDLDVESDFDDASI
S318 ESDFDDASINSYSVS
S321 FDDASINSYSVSDGS
Y322 DDASINSYSVSDGST
S323 DASINSYSVSDGSTS
T360 TAVDGYETDHQDYCE
S421-p WEAKEDNsEGEEILE
K497 ALKGKVQKILIWKWG
S508 WKWGQPPSPTPVPRP
T510 WGQPPSPTPVPRPPD
T522 PPDADPNTPSPKPLE
S524 DADPNTPSPKPLEGR
T696 KLERPPETPTVDPTV
T698 ERPPETPTVDPTVKY
T754 GLGKTVQTAVFLYSL
Y759 VQTAVFLYSLYKEGH
S760 QTAVFLYSLYKEGHS
Y762 AVFLYSLYKEGHSKG
Y978-p LSPMQKKyYKYILTR
K1009 LNVVMDLKKCCNHPY
K1009 LNVVMDLKKCCNHPY
K1010 NVVMDLKKCCNHPYL
K1050 GKLLLLQKMLKNLKE
K1085 FLEHEGYKYERIDGG
T1200 RPGLGSKTGSMSKQE
S1202 GLGSKTGSMSKQELD
D1226 LFKDEATDGGGDNKE
K1245 SVIHYDDKAIERLLD
Y1269 ELQGMNEYLSSFKVA
K1297 EVEREIIKQEESVDP
S1301 EIIKQEESVDPDYWE
S1342 QVNYNDGSQEDRDWQ
S1353 RDWQDDQSDNQSDYS
S1360 SDNQSDYSVASEEGD
S1363 QSDYSVASEEGDEDF
S1493 LTRIGVMSLIRKKVQ
S1524-p VEENKKMsQPGsPSP
S1528-p KKMsQPGsPSPKTPT
S1530 MsQPGsPSPKTPTPS
T1533 PGsPSPKTPTPSTPG
T1535 sPSPKTPTPSTPGDT
S1537 SPKTPTPSTPGDTQP
T1538 PKTPTPSTPGDTQPN
T1542 TPSTPGDTQPNTPAP
T1546 PGDTQPNTPAPVPPA
S1563 GIKIEENSLKEEEST
K1565 KIEENSLKEEESTEG
S1569 NSLKEEESTEGEKEV
K1577 TEGEKEVKPTAPEVT
- gap
K1652 KGTTEVEKAEEKSAV
K1656 EVEKAEEKSAVDLTP
S1657 VEKAEEKSAVDLTPI
T1662 EKSAVDLTPIVVEDK
T1688 VLLQNGETPKDLSDE
S1848 HQHLSKESMAGNKPA
K1861 PANAVLHKVLKQLEE
K1864 AVLHKVLKQLEELLS
K1874 EELLSDMKADVTRLP
P1913 ANRAPEPPPQQVAQQ
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