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Protein Page:
PFKP (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
PFKP phosphofructokinase, platelet type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Note: This description may include information from UniProtKB.
Protein type: Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; Carbohydrate Metabolism - galactose; EC 2.7.1.11; Carbohydrate Metabolism - fructose and mannose
Cellular Component: 6-phosphofructokinase complex; cytosol
Molecular Function: metal ion binding; ATP binding; 6-phosphofructokinase activity
Biological Process: fructose 1,6-bisphosphate metabolic process; glycolysis; carbohydrate metabolic process; glucose metabolic process; fructose 6-phosphate metabolic process; protein homotetramerization
Reference #:  Q01813 (UniProtKB)
Alt. Names/Synonyms: 6-phosphofructokinase type C; 6-phosphofructokinase, platelet type; FLJ40226; K6PP; PFK-C; PFKF; PFKP; Phosphofructo-1-kinase isozyme C; Phosphofructokinase 1; phosphofructokinase, platelet; Phosphofructokinase, platelet type; Phosphohexokinase
Gene Symbols: PFKP
Molecular weight: 85,596 Da
Basal Isoelectric point: 7.5  Predict pI for various phosphorylation states
CST Pathways:  Warburg Effect
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PFKP
Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

 
0 2 D2 ______MDADDSRAP
1 1 S12-p DSRAPKGsLRkFLEH
0 2 K15-u APKGsLRkFLEHLsG
0 1 S21-p RkFLEHLsGAGkAIG
0 1 K25-u EHLsGAGkAIGVLTS
0 1 K109-u RTREGRLkAACNLLQ
0 3 K139-u TGANLFRkEWsGLLE
0 2 S142-p NLFRkEWsGLLEELA
0 4 K156-u ARNGQIDkEAVQKYA
0 3 K281 GAIDTQNKPITSEkI
0 3 K287-u NKPITSEkIkELVVT
0 2 K289-u PITSEkIkELVVTQL
0 1 T294 kIkELVVTQLGYDTR
0 2 T313 GHVQRGGTPSAFDRI
0 191 S386-p AVRLRGRsFAGNLNT
0 16 K395-a AGNLNTYkRLAIkLP
0 13 K395-u AGNLNTYkRLAIkLP
0 2 K400-u TYkRLAIkLPDDQIP
0 7 Y447-p GHRMLAIyDGFDGFA
0 1 K455-u DGFDGFAkGQIkEIG
0 2 K459-u GFAkGQIkEIGWTDV
0 16 K480-a GGSILGTkRVLPGky
0 1 K480-u GGSILGTkRVLPGky
0 3 K486-a TkRVLPGkyLEEIAT
0 2 K486-u TkRVLPGkyLEEIAT
0 18 Y487-p kRVLPGkyLEEIATQ
0 4 K574-a KQSASGTkRRVFIIE
0 3 Y586-p IIETMGGyCGYLANM
0 1 K625-u NVEHLTEkMKTTIQR
0 1 Y645-p NESCSENyTTDFIyQ
0 57 Y651-p NyTTDFIyQLySEEG
0 1 Y654-p TDFIyQLySEEGKGV
0 1 K666-u KGVFDCRkNVLGHMQ
0 2 K688-a FDRNFGTkISARAME
0 14 K688-u FDRNFGTkISARAME
0 1 R692 FGTkISARAMEWITA
0 1 K700-a AMEWITAkLKEARGR
0 1 T713 GRGKKFTTDDSICVL
0 13 K736-a FQPVAELkKQTDFEH
0 1 K736-u FQPVAELkKQTDFEH
0 1 K747-a DFEHRIPkEQWWLKL
0 3 K759 LKLRPLMKILAKYkA
0 1 K765-u MKILAKYkASYDVSD
0 1 Q775 YDVSDSGQLEHVQPW
0 1 H778 SDSGQLEHVQPWsV_
0 9 S783-p LEHVQPWsV______
  mouse

 
S2-p ______MsDLDSSSS
A11 LDSSSSSAYPkYLEH
K14-u SSSSAYPkYLEHLSG
S20 PkYLEHLSGDGKAIG
K24 EHLSGDGKAIGVLTS
K108 RSREGRLKAACNLAR
K138-u TGANLFRkEWsGLLE
S141-p NLFRkEWsGLLEELA
N155 ARNGDIDNDTVQKYS
K280 GAIDMQNKPITSEkI
K286-u NKPITSEkIkELVVk
K288-u PITSEkIkELVVkNL
K293-u kIkELVVkNLGFDTR
T312-p GHVQRGGtPSAFDRI
R385 AVKLRGRRFEGNLNT
K394 EGNLNTYKRLAIKLP
K394-u EGNLNTYkRLAIKLP
K399 TYkRLAIKLPDEKIV
Y446 GHKMFAIYDGFEGFA
N454 DGFEGFANGQIKEIG
K458 GFANGQIKEIGWADV
K479 GGSILGTKRTLPGKY
K479 GGSILGTKRTLPGKY
K485 TKRTLPGKYLEKIAE
K485 TKRTLPGKYLEKIAE
Y486 KRTLPGKYLEKIAEQ
K573 KQSASGTKRRVFIIE
Y585 IIETMGGYCGYLANM
K624 NVVHLTEKMKTSIQR
Y644 NESCSVNYTTDFIyQ
Y650-p NYTTDFIyQLYSEEG
Y653 TDFIyQLYSEEGKGV
K665 KGVFDCRKNVLGHMQ
K687 FDRNFGTKISAkAME
K687-u FDRNFGTkISAkAME
K691-u FGTkISAkAMEWISA
K699 AMEWISAKLKGSQGT
S712-p GTGKKFVsDDSICVL
K735 FQPVAELKKVTDFEH
K735 FQPVAELKKVTDFEH
K746 DFEHRIPKEQWWLKL
K758 LKLRPIMKILAKYEA
E764 MKILAKYEASYDMSD
K774-u YDMSDSGkLEsLQHH
S777-p SDSGkLEsLQHHEEL
E782 LEsLQHHEEL_____
  rat

 
S2 ______MSDQDSSTS
S12 DSSTSSTSFPKYLEH
K15 TSSTSFPKYLEHLSG
S21 PKYLEHLSGDGKAIG
K25 EHLSGDGKAIGVLTS
K109 RSREGRLKAACNLVR
K139 TGANLFRKEWSGLLE
S142 NLFRKEWSGLLEELA
S156 AKNGEIDSDTVKKHA
K281-u GAIDTQNkPITSEkI
K287-u NkPITSEkIKELVVT
K289 PITSEkIKELVVTNL
T294 kIKELVVTNLGFDTR
T313 GHVQRGGTPSAFDRI
S386 AVKLRGRSFEGNLNT
K395 EGNLNTYKRLAIKEP
K395-u EGNLNTYkRLAIKEP
K400 TYkRLAIKEPDDKIP
Y447 GHKMFAIYDGFDGLA
N455 DGFDGLANGQIKEIG
K459 GLANGQIKEIGWGDV
K480-a GGSILGTkRTLPGKY
K480 GGSILGTKRTLPGKY
K486 TkRTLPGKYLEKIAE
K486 TkRTLPGKYLEKIAE
Y487 kRTLPGKYLEKIAEQ
K574 KQSASGTKRRVFIIE
Y586 IIETMGGYCGYLANM
K625 NVMHLTEKMKTSIQR
Y645 NENCSVNYTTDFIYQ
Y651 NYTTDFIYQLYSEEG
Y654 TDFIYQLYSEEGKGV
K666 KGVFDCRKNVLGHMQ
K688 FDRNFGTKISAKAME
K688-u FDRNFGTkISAKAME
K692 FGTkISAKAMEWISA
K700 AMEWISAKLKGSHGT
S713 GTGKKFVSDDSICVL
R736 FKPVAELRKATDFEH
R736 FKPVAELRKATDFEH
K747 DFEHRIPKQQWWLKL
K759-u LKLRPIMkILAKYEA
E765 MkILAKYEASYDMSD
K775 YDMSDVGKLEPVHNH
P778 SDVGKLEPVHNHGEL
G783 LEPVHNHGELSAI__
  rabbit

 
D2 ______MDNKVSASP
S12-p VSASPRGsYRKFLEH
K15 SPRGsYRKFLEHLSG
S21 RKFLEHLSGAGKAIG
K25 EHLSGAGKAIGVLTS
K109 RTREGRLKAACNLIH
M139 TGANIFRMEWSGLLE
S142 NIFRMEWSGLLEELA
N156 AQDGKIDNEAVQKYA
R281 GAIDTLNRPITSEKI
K287 NRPITSEKIKELVVT
K289 PITSEKIKELVVTQL
T294 KIKELVVTQLGYDTR
T313 GHVQRGGTPSAFDRI
S386 AVQLRGRSFENNLNT
K395 ENNLNTYKRLAIKLP
K395 ENNLNTYKRLAIKLP
K400 TYKRLAIKLPDDKIQ
Y447 GHKMFAVYDGFDGFA
K455 DGFDGFAKGQIKEIR
K459 GFAKGQIKEIRWGDV
K480 GGSILGTKRILPGKY
K480 GGSILGTKRILPGKY
K486 TKRILPGKYLEEIAT
K486 TKRILPGKYLEEIAT
Y487 KRILPGKYLEEIATQ
K574 KQSASGTKRRVFIIE
Y586 IIETMGGYCGYLANM
K625 NVEHLTEKMKTTIQR
Y645 NESCSENYTTDFIYQ
Y651 NYTTDFIYQLYSEEG
Y654 TDFIYQLYSEEGRGV
K666 RGVFDCRKNVLGHMQ
K688 FDRNFGTKISARAMQ
K688 FDRNFGTKISARAMQ
R692 FGTKISARAMQWITT
K700 AMQWITTKLKESPGK
S713 GKGKRFVSDDSICVL
K736 FQPVAELKNETDFEH
K736 FQPVAELKNETDFEH
K747 DFEHRIPKEQWWLKL
K759 LKLRPLMKILAKYKT
K765 MKILAKYKTSYDVSD
Q775 YDVSDSGQLVPVRHR
P778 SDSGQLVPVRHRGGP
G783 LVPVRHRGGPEEPAA
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